Molecular Oral Microbiology最新文献_第7页

miR-27a-5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten. miR-27a-5p通过靶向10号染色体上缺失的磷酸酶和紧张素同源物来缓解牙周炎症。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-08-01 DOI: 10.1111/omi.12416

Li Deng, Peng-Cheng Huo, Mei-Ting Feng, Rui-Ling Wang, Rui Jing, Li-Jun Luo

{"title":"miR-27a-5p alleviates periodontal inflammation by targeting phosphatase and tensin homolog deleted on chromosome ten.","authors":"Li Deng, Peng-Cheng Huo, Mei-Ting Feng, Rui-Ling Wang, Rui Jing, Li-Jun Luo","doi":"10.1111/omi.12416","DOIUrl":"https://doi.org/10.1111/omi.12416","url":null,"abstract":"Introduction: MicroRNAs (miRNAs), a type of non-coding RNA, have been demonstrated to be essential posttranscriptional modulators in oral diseases and inflammatory responses. However, the specific role of miR-27a-5p in periodontitis requires further investigation. In this study, we used both cellular and animal models to determine how miR-27a-5p affects the pathogenesis of periodontitis and its associated biological functions.Methods: Quantitative real-time polymerase chain reaction and western blotting were used to analyze the expression of cytokines, phosphatase and tensin homolog deleted on chromosome ten (PTEN), and miR-27a-5p transcription. Investigation of alveolar bone resorption and inflammation of the periodontium in ligature-induced periodontitis in mice was performed using micro-computed tomography (micro-CT), hematoxylin-eosin (HE) staining, and tartrate-resistant acid phosphatase (TRAP) staining. The binding of miR-27a-5p and PTEN was predicted using the TargetScan database and experimentally confirmed using dual luciferase reporter gene assays.Results: The inflamed gingiva showed lower levels of miR-27a-5p. Macrophages from miR-27a-5p-/- mice produced much higher quantities of pro-inflammatory cytokines owing to the stimulation of Porphyromonas gingivalis lipopolysaccharide, and miR-27a-5p-/- mice with ligature-induced periodontitis also exhibited more severe alveolar bone resorption and damage to the periodontium. Target validation assays identified PTEN as a direct target of bona. Blocking PTEN expression partially reduced inflammation, both in vitro and in vivo.Conclusions: miR-27a-5p alleviated the inflammatory response in periodontitis by targeting PTEN.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 4","pages":"309-320"},"PeriodicalIF":3.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10152302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human papillomavirus and Epstein-Barr virus co-infection in oral and oropharyngeal squamous cell carcinomas: A systematic review and meta-analysis. 人乳头瘤病毒和eb病毒在口腔和口咽鳞状细胞癌中的联合感染:一项系统综述和荟萃分析

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-08-01 DOI: 10.1111/omi.12412

Rifat Rahman, Mushfiq H Shaikh, Divya Gopinath, Adi Idris, Newell W Johnson

{"title":"Human papillomavirus and Epstein-Barr virus co-infection in oral and oropharyngeal squamous cell carcinomas: A systematic review and meta-analysis.","authors":"Rifat Rahman, Mushfiq H Shaikh, Divya Gopinath, Adi Idris, Newell W Johnson","doi":"10.1111/omi.12412","DOIUrl":"https://doi.org/10.1111/omi.12412","url":null,"abstract":"Squamous cell carcinoma of the oral cavity (OSCC) is the most common head-and-neck malignancy. Importantly, we are experiencing an alarming rise in the incidence of oropharyngeal squamous cell carcinoma (OPSCC) globally. Oncogenic viruses, human papillomavirus (HPV) and Epstein-Barr virus (EBV), are known to be co-associated with OSCC and OPSCC cases. However, the reported incidence of HPV and EBV co-infection in OSCCs and OPSCCs globally is unknown. To address this, we performed a formal meta-analysis and systematic review on published studies that report the detection of both EBV and HPV in OSCCs and OPSCCs. Our analysis revealed 18 relevant studies out of a total of 1820 cases (1181 from the oral cavity and 639 from the oropharynx). Overall, HPV and EBV co-infection was found in 11.9% of OSCC and OPSCC cases combined (95% CI: 8%-14.1%). Based on anatomical subsite, dual positivity estimates were 10.5% (95% CI: 6.7%-15.1%) for OSCC and 14.2% (95% CI: 9.1%-21.3%) for OPSCC. The highest dual positivity rates described were in European countries: for OSCC 34.7% (95% CI: 25.9%-44.6%) in Sweden and for OPSCC, 23.4% (95% CI: 16.9%-31.5%) in Poland. Given these substantive prevalence rates, the value of detecting dual infection in the diagnosis and prognosis of these cancers deserves careful longitudinal studies, as do implications for cancer prevention and therapy. We further proposed molecular mechanisms that could explain how HPV and EBV could co-contribute to the aetiology of OSCCs and OPSCCs.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 4","pages":"259-274"},"PeriodicalIF":3.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9787107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The involvement of CdhR in Porphyromonas gingivalis during nitric oxide stress. 一氧化氮应激期间牙龈卟啉单胞菌中 CdhR 的参与。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-08-01 Epub Date: 2023-05-03 DOI: 10.1111/omi.12414

Marie-Claire Boutrin, Arunima Mishra, Charles Wang, Yuetan Dou, Hansel M Fletcher

{"title":"The involvement of CdhR in Porphyromonas gingivalis during nitric oxide stress.","authors":"Marie-Claire Boutrin, Arunima Mishra, Charles Wang, Yuetan Dou, Hansel M Fletcher","doi":"10.1111/omi.12414","DOIUrl":"10.1111/omi.12414","url":null,"abstract":"Porphyromonas gingivalis, the causative agent of adult periodontitis, must gain resistance to frequent oxidative and nitric oxide (NO) stress attacks from immune cells in the periodontal pocket to survive. Previously, we found that, in the wild-type and under NO stress, the expression of PG1237 (CdhR), the gene encoding for a putative LuxR transcriptional regulator previously called community development and hemin regulator (CdhR), was upregulated 7.7-fold, and its adjacent gene PG1236 11.9-fold. Isogenic mutants P. gingivalis FLL457 (ΔCdhR::ermF), FLL458 (ΔPG1236::ermF), and FLL459 (ΔPG1236-CdhR::ermF) were made by allelic exchange mutagenesis to determine the involvement of these genes in P. gingivalis W83 NO stress resistance. The mutants were black pigmented and β hemolytic and their gingipain activities varied with strains. FLL457 and FLL459 mutants were more sensitive to NO compared to the wild type, and complementation restored NO sensitivity to that of the wild type. DNA microarray analysis of FLL457 showed that approximately 2% of the genes were upregulated and over 1% of the genes downregulated under NO stress conditions compared to the wild type. Transcriptome analysis of FLL458 and FLL459 under NO stress showed differences in their modulation patterns. Some similarities were also noticed between all mutants. The PG1236-CdhR gene cluster revealed increased expression under NO stress and may be part of the same transcriptional unit. Recombinant CdhR showed binding activity to the predicted promoter regions of PG1459 and PG0495. Taken together, the data indicate that CdhR may play a role in NO stress resistance and be involved in a regulatory network in P. gingivalis.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 4","pages":"289-308"},"PeriodicalIF":3.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11018363/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9792413","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gingipains are the important virulence factors of Porphyromonas gingivalis downregulating B10 cells. 牙龈痛是下调B10细胞的牙龈卟啉单胞菌的重要毒力因子。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-08-01 DOI: 10.1111/omi.12413

Hang Zou, Niu Zhou, Xiao Cheng, Yi Qiu, Wenhong Hou, Jianbo Sun

{"title":"Gingipains are the important virulence factors of Porphyromonas gingivalis downregulating B10 cells.","authors":"Hang Zou, Niu Zhou, Xiao Cheng, Yi Qiu, Wenhong Hou, Jianbo Sun","doi":"10.1111/omi.12413","DOIUrl":"https://doi.org/10.1111/omi.12413","url":null,"abstract":"Porphyromonas gingivalis is a keystone pathogen in periodontitis. Our previous study indicated that periodontitis induced by P. gingivalis increased the percentage of CD19+ B cells but decreased the ratio of IL-10-producing regulatory B cells (B10) in collagen-induced arthritis (CIA) mice. It is still unclear which virulence factors of P. gingivalis are involved in these processes. Here, we compared the effects of different components of P. gingivalis on the biogenesis of B10 cells and found that the decreased proportion of B10 cells mainly resulted from the undenatured proteins other than the DNA, RNA, or lipopolysaccharides of P. gingivalis. As gingipains are enzymes and virulence factors that play a vital role in the progression in periodontitis through affecting the innate and adaptive immune system, we then compared the influence of the wild-type (WT) strain of P. gingivalis (ATCC 33277) and its isogenic gingipain-null mutant (∆K∆RAB) on the differentiation of splenic B cells into B10 cells. Interestingly, compared to WT strain, ∆K∆RAB treatment increased the frequency of B10 cells as well as the expression of IL-6 in B cells. Furthermore, the acute peritonitis, an ideal model for the quick evaluation of immune effects of agents, induced by ∆K∆RAB, showed the higher IL-6 production and proportion of B10 cells compared with WT. Finally, we performed transcriptomic analysis to better understand the effects and possible mechanisms of gingipains on B cells. Compared with WT, ∆K∆RAB upregulated the PI3K-Akt pathway of B cells, which is important for IL-10 production and B10 cell biogenesis, and more activated Jak-STAT pathway, which is a classical signaling pathway mediated by IL-6. Cumulatively, this study preliminarily revealed that gingipains of P. gingivalis are vital virulence factors downregulating B10 cells and altering immune responses.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 4","pages":"275-288"},"PeriodicalIF":3.7,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9780467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

vicR overexpression in Streptococcus mutans causes aggregation and affects interspecies competition. 变形链球菌中vicR的过表达引起聚集并影响种间竞争。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 DOI: 10.1111/omi.12407

Jiangchuan Yan, Tao Gong, Qizhao Ma, Ting Zheng, Jiamin Chen, Jing Li, Meiling Jing, Yongwang Lin, Xiaowan Wang, Lei Lei, Shida Wang, Jumei Zeng, Yuqing Li

{"title":"vicR overexpression in Streptococcus mutans causes aggregation and affects interspecies competition.","authors":"Jiangchuan Yan, Tao Gong, Qizhao Ma, Ting Zheng, Jiamin Chen, Jing Li, Meiling Jing, Yongwang Lin, Xiaowan Wang, Lei Lei, Shida Wang, Jumei Zeng, Yuqing Li","doi":"10.1111/omi.12407","DOIUrl":"https://doi.org/10.1111/omi.12407","url":null,"abstract":"Streptococcus mutans is considered to be a major causative agent of dental caries. VicRK is a two-component signal transduction system (TCSTS) of S. mutans, which can regulate the virulence of S. mutans, such as biofilm formation, exopolysaccharide production, acid production, and acid resistance. Meanwhile, it can also regulate the production of mutacins (nlmC) through the TCSTS ComDE. In this study, we found that the vicR-overexpressing strain was more likely to aggregate to form cell clusters, leading to the formation of abnormal biofilm; the overexpression of vicR increased the length of the chain of S. mutans. Furthermore, the expression of the mutacins in the vicR overexpression strain was increased under aerobic conditions. Compared with the control strain and the parental strain, the vicR overexpression strain was more competitive against Streptococcus gordonii. But there was no significant difference against Streptococcus sanguinis. In clinical strains, the expression level of vicR was positively correlated with their competitive ability against S. gordonii. Transcriptional profiling revealed 24 significantly upregulated genes in the vicR-overexpressing strain, including nlmA, nlmB, nlmC, and nlmD encoding mutacins. Electrophoretic mobility shift assays and DNase I footprinting assays confirmed that VicR can directly bind to the promoter sequence of nlmD. Taken together, our findings further demonstrate that VicRK, an important TCSTS of S. mutans, is involved in S. mutans cell morphology and biofilm formation. VicRK regulates the production of more mutacins in S. mutans in response to oxygen stimulation. VicR can bind to the promoter sequence of nlmD, thereby directly regulating the production of mutacins NlmD.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 3","pages":"224-236"},"PeriodicalIF":3.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455941","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Regulation of adhesin synthesis in Aggregatibacter actinomycetemcomitans. 放线菌中粘附素合成的调节。

IF 2.8 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 Epub Date: 2023-03-15 DOI: 10.1111/omi.12410

Jake Tristano, David R Danforth, Matthew J Wargo, Keith P Mintz

{"title":"Regulation of adhesin synthesis in Aggregatibacter actinomycetemcomitans.","authors":"Jake Tristano, David R Danforth, Matthew J Wargo, Keith P Mintz","doi":"10.1111/omi.12410","DOIUrl":"10.1111/omi.12410","url":null,"abstract":"Aggregatibacter actinomycetemcomitans is a gram-negative bacterium associated with periodontal disease and a variety of disseminated extra-oral infections. Tissue colonization is mediated by fimbriae and non-fimbriae adhesins resulting in the formation of a sessile bacterial community or biofilm, which confers enhanced resistance to antibiotics and mechanical removal. The environmental changes experienced by A. actinomycetemcomitans during infection are detected and processed by undefined signaling pathways that alter gene expression. In this study, we have characterized the promoter region of the extracellular matrix protein adhesin A (EmaA), which is an important surface adhesin in biofilm biogenesis and disease initiation using a series of deletion constructs consisting of the emaA intergenic region and a promotor-less lacZ sequence. Two regions of the promoter sequence were found to regulate gene transcription and in silico analysis indicated the presence of multiple transcriptional regulatory binding sequences. Analysis of four regulatory elements, CpxR, ArcA, OxyR, and DeoR, was undertaken in this study. Inactivation of arcA, the regulator moiety of the ArcAB two-component signaling pathway involved in redox homeostasis, resulted in a decrease in EmaA synthesis and biofilm formation. Analysis of the promoter sequences of other adhesins identified binding sequences for the same regulatory proteins, which suggests that these proteins are involved in the coordinate regulation of adhesins required for colonization and pathogenesis.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 3","pages":"237-250"},"PeriodicalIF":2.8,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175207/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy of Cetylpyridinium Chloride mouthwash against SARS-CoV-2: A systematic review of randomized controlled trials. 氯化十六烷基吡啶漱口水对SARS-CoV-2的疗效:随机对照试验的系统评价。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 DOI: 10.1111/omi.12408

Filippo D'Amico, Matteo Moro, Marco Saracino, Marilena Marmiere, Maria Bernadette Cilona, Graham Lloyd-Jones, Alberto Zangrillo

{"title":"Efficacy of Cetylpyridinium Chloride mouthwash against SARS-CoV-2: A systematic review of randomized controlled trials.","authors":"Filippo D'Amico, Matteo Moro, Marco Saracino, Marilena Marmiere, Maria Bernadette Cilona, Graham Lloyd-Jones, Alberto Zangrillo","doi":"10.1111/omi.12408","DOIUrl":"https://doi.org/10.1111/omi.12408","url":null,"abstract":"Introduction: COVID-19 is a transmissible respiratory and multisystem disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Viral transmission occurs mainly through the spread of salivary droplets or aerosol from an infected subject. Studies suggest that salivary viral load is correlated with disease severity and probability of transmission. Cetylpyridinium chloride mouthwash has been found to be effective in reducing salivary viral load. The aim of this systematic review of randomized controlled trials is to evaluate the efficacy of the mouthwash ingredient cetylpyridinium chloride on salivary viral load in SARS-CoV-2 infection.Methods: Randomized controlled trials comparing cetylpyridinium chloride mouthwash with placebo and other mouthwash ingredients in SARS-CoV-2 positive individuals were identified and evaluated.Results: Six studies with a total of 301 patients that met the inclusion criteria were included. The studies reported the efficacy of cetylpyridinium chloride mouthwashes in reduction on SARS-CoV-2 salivary viral load compared to placebo and other mouthwash ingredients.Conclusion: Mouthwashes containing cetylpyridinium chloride are effective against salivary viral load of SARS-CoV-2 in vivo. There is also the possibility that the use of mouthwash containing cetylpyridinium chloride in SARS-CoV-2 positive subjects could reduce transmissibility and severity of COVID-19.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 3","pages":"171-180"},"PeriodicalIF":3.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9449182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Metabolism of periodontal pathobionts: Their regulatory roles in the dysbiotic microbiota. 牙周病原体的代谢:它们在益生菌群中的调节作用。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 DOI: 10.1111/omi.12409

Jing Ding, Chuanjiang Zhao, Li Gao

引用次数: 1

Dental calculus microbiome correlates with dietary intake. 牙石菌群与饮食摄入相关。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 DOI: 10.1111/omi.12404

Gabriel Innocenti, Maria Elena Martino, Edoardo Stellini, Adolfo Di Fiore, Andrea Quagliariello

{"title":"Dental calculus microbiome correlates with dietary intake.","authors":"Gabriel Innocenti, Maria Elena Martino, Edoardo Stellini, Adolfo Di Fiore, Andrea Quagliariello","doi":"10.1111/omi.12404","DOIUrl":"https://doi.org/10.1111/omi.12404","url":null,"abstract":"Background: Dental calculus is the result of dental plaque mineralization, originating from the tooth-associated bacterial biofilm. Recent evidence revealed that the dental calculus microbiome has a more complex composition than previously considered, including an unstructured mix of both aerobes and anaerobes bacteria. Actually, we lack information about the influence of host lifestyle factors, such as diet and health on this highly biodiverse ecosystem. Here, we provide a pilot study investigating dental calculus microbial biodiversity and its relation with the host diet.Methods: We collected 40 dental calculus samples during routine dental inspection; deoxyribonucleic acid was extracted and analyzed through 16S amplicon sequencing, while dietary information was retrieved through a questionnaire. Associations between diet and oral bacteria taxonomy and functional pathways were statistically tested.Results: Overall, microbiome composition was dominated by 10 phyla and 39 bacterial genera, which were differently distributed among samples. Cluster analysis revealed four main groups based on the taxonomic profile and two groups based on functional pathways. Each taxonomic cluster was dominated by different microbial biomarkers: Streptococcus, Rothia, Tannerella, Lautropia, and Fusobacterium. Bacteria genera and pathways were also associated with specific dietary elements, especially vegetable and fruit intake suggesting an overall effect of diet on dental calculus microbiome.Conclusions: The present study demonstrates that there exists an inter-variability in the microbial composition of dental calculus among individuals of a rather homogeneous population. Furthermore, the observed biodiversity and microbial functions can find an association with specific dietary habits, such as a high-fiber diet or a protein-rich diet.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 3","pages":"189-197"},"PeriodicalIF":3.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9808917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Localization and pathogenic role of the cysteine protease dentipain in Treponema denticola. 半胱氨酸蛋白酶牙痛在密螺旋体中的定位和致病作用。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2023-06-01 Epub Date: 2023-01-28 DOI: 10.1111/omi.12406

Yuri Miyai-Murai, Kazuko Okamoto-Shibayama, Toru Sato, Yuichiro Kikuchi, Eitoyo Kokubu, Jan Potempa, Kazuyuki Ishihara

{"title":"Localization and pathogenic role of the cysteine protease dentipain in Treponema denticola.","authors":"Yuri Miyai-Murai, Kazuko Okamoto-Shibayama, Toru Sato, Yuichiro Kikuchi, Eitoyo Kokubu, Jan Potempa, Kazuyuki Ishihara","doi":"10.1111/omi.12406","DOIUrl":"10.1111/omi.12406","url":null,"abstract":"The Msp protein complex and the serine protease dentilisin are the best-characterized virulence factors in Treponema denticola, the major etiological agent of chronic periodontitis. In addition to these outer sheath factors, the cysteine protease dentipain contributes to pathogenicity, but its secretion, processing, cellular localization, and role in T. denticola virulence are not fully understood. In this study, we found that full-sized dentipain (74-kDa) and the 52-kDa truncated form of the enzyme are located, respectively, in the outer sheath derived from T. denticola dentilisin- and the Msp-deficient mutants. Furthermore, dentipain was barely detected in the wild-type strain. These results suggest that dentilisin and Msp, the major outer sheath proteins, are involved in the secretion and maturation of dentipain. Inactivation of the dentipain gene slowed the growth of T. denticola, and the effect was more profound in serum-free medium than in serum-containing medium. Several genes, including those encoding transporters and methyl-accepting chemotaxis proteins, were differentially expressed in the dentipain-deficient mutant. Furthermore, the mutant strain was more hydrophobic than the wild-type strain. Finally, the mutant showed less autoaggregation activity and adhesion to IgG in a serum-free medium than the wild-type strain. These findings suggest that dentipain contributes to the virulence of T. denticola by facilitating adhesion and acquisition of nutrients essential for colonization and proliferation in the gingival crevice under serum-rich conditions.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"38 3","pages":"212-223"},"PeriodicalIF":3.7,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10175099/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9455424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0