Molecular Oral Microbiology最新文献_第10页

Electrochemical sensors for oral biofilm-biomaterials interface characterization: A review. 口腔生物膜-生物材料界面电化学传感器研究进展。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-12-01 Epub Date: 2022-11-15 DOI: 10.1111/omi.12396

Dipankar Koley

{"title":"Electrochemical sensors for oral biofilm-biomaterials interface characterization: A review.","authors":"Dipankar Koley","doi":"10.1111/omi.12396","DOIUrl":"10.1111/omi.12396","url":null,"abstract":"Important processes related to the interaction of the oral microbiome with the tooth surface happen directly at the interface. For example, the chemical microenvironment that exists at the interface of microbial biofilms and the native tooth structure is directly involved in caries development. Consequentially, a critical understanding of this interface and its chemical microenvironment would provide novel avenues in caries prevention, including secondary caries that often occurs at the interface of the dental biofilm, tooth structure, and dental material. Electrochemical sensors are a unique quantitative tool and have the inherent advantages of miniaturization, stability, and selectivity. That makes the electrochemical sensors ideal tools for studying these critical biofilm microenvironments with high precision. This review highlights the development and applications of several novel electrochemical sensors such as pH, Ca2+ , and hydrogen peroxide sensors as scanning electrochemical microscope probes in addition to flexible pH wire sensors for real-time bacterial biofilm-dental surface and dental materials interface studies.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"37 6","pages":"292-298"},"PeriodicalIF":3.7,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9759506/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10729870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover Image, Volume 37, Issue 6 封面图片，第37卷，第6期

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-11-24 DOI: 10.1111/omi.12401

引用次数: 0

The functional oral microbiome: Biofilm environment, polymicrobial interactions, and community dynamics. 功能性口腔微生物群：生物膜环境、多微生物相互作用和群落动态。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-10-01 DOI: 10.1111/omi.12390

Jens Kreth, Hyun Koo, Patricia I Diaz

引用次数: 0

A cell wall-anchored glycoprotein confers resistance to cation stress in Actinomyces oris biofilms. 细胞壁锚定的糖蛋白赋予口放线菌生物膜对阳离子应激的抗性。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-10-01 Epub Date: 2022-03-25 DOI: 10.1111/omi.12365

Abu Amar M Al Mamun, Chenggang Wu, Chungyu Chang, Belkys C Sanchez, Asis Das, Hung Ton-That

{"title":"A cell wall-anchored glycoprotein confers resistance to cation stress in Actinomyces oris biofilms.","authors":"Abu Amar M Al Mamun, Chenggang Wu, Chungyu Chang, Belkys C Sanchez, Asis Das, Hung Ton-That","doi":"10.1111/omi.12365","DOIUrl":"https://doi.org/10.1111/omi.12365","url":null,"abstract":"Actinomyces oris plays an important role in oral biofilm development. Like many gram-positive bacteria, A. oris produces a sizable number of surface proteins that are anchored to bacterial peptidoglycan by a conserved transpeptidase named the housekeeping sortase SrtA; however, the biological role of many A. oris surface proteins in biofilm formation is largely unknown. Here, we report that the glycoprotein GspA-a genetic suppressor of srtA deletion lethality-not only promotes biofilm formation but also maintains cell membrane integrity under cation stress. In comparison to wild-type cells, under elevated concentrations of mono- and divalent cations the formation of mono- and multi-species biofilms by mutant cells devoid of gspA was significantly diminished, although planktonic growth of both cell types in the presence of cations was indistinguishable. Because gspA overexpression is lethal to cells lacking gspA and srtA, we performed a genetic screen to identify GspA determinants involving cell viability. DNA sequencing and biochemical characterizations of viable clones revealed that mutations of two critical cysteine residues and a serine residue severely affected GspA glycosylation and biofilm formation. Furthermore, mutant cells lacking gspA were markedly sensitive to sodium dodecyl sulfate, a detergent that solubilizes the cytoplasmic membranes, suggesting the cell envelope of the gspA mutant was altered. Consistent with this observation, the gspA mutant exhibited increased membrane permeability, independent of GspA glycosylation, compared to the wild-type strain. Altogether, the results support the notion that the cell wall-anchored glycoprotein GspA provides a defense mechanism against cation stress in biofilm development promoted by A. oris.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"37 5","pages":"206-217"},"PeriodicalIF":3.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9474737/pdf/nihms-1788776.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41134859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A reappraisal of microbiome dysbiosis during experimental periodontitis. 实验性牙周炎期间微生物群失调的重新评估。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-10-01 DOI: 10.1111/omi.12382

Marion Arce, Natalia Endo, Nicolas Dutzan, Loreto Abusleme

{"title":"A reappraisal of microbiome dysbiosis during experimental periodontitis.","authors":"Marion Arce, Natalia Endo, Nicolas Dutzan, Loreto Abusleme","doi":"10.1111/omi.12382","DOIUrl":"https://doi.org/10.1111/omi.12382","url":null,"abstract":"Periodontitis is a chronic inflammatory disease associated with the presence of dysbiotic microbial communities. Several studies interrogating periodontitis pathogenesis have utilized the murine ligature-induced periodontitis (LIP) model and have further examined the ligature-associated microbiome relying on 16S rRNA-based sequencing techniques. However, it is often very challenging to compare microbial profiles across studies due to important differences in bioinformatic processing and databases used for taxonomic assignment. Thus, our study aim was to reanalyze microbiome sequencing datasets from studies utilizing the LIP model through a standardized bioinformatic analysis pipeline, generating a comprehensive overview of microbial dysbiosis during experimental periodontitis.We conducted a reanalysis of 16S rDNA gene sequencing datasets from nine published studies utilizing the LIP model. Reads were grouped according to the hypervariable region of the 16S rDNA gene amplified (V1-V3 and V4), preprocessed, binned into operational taxonomic units and classified utilizing relevant databases. Alpha- and beta-diversity analyses were conducted, along with relative abundance profiling of microbial communities. Our findings revealed similar microbial richness and diversity across studies and determined shifts in microbial community structure determined by periodontitis induction and study of origin. Clear variations in the relative abundance of bacterial taxa were observed starting on day 5 after ligation and onward, consistent with a distinct microbial composition during health and experimental periodontitis. We also uncovered differentially represented bacterial taxa across studies, dominating periodontal health and LIP-associated communities. Collectively, this reanalysis provides a unified overview of microbial dysbiosis during the LIP model, providing new insights that aim to inform further studies dedicated to unraveling oral host-microbial interactions.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"37 5","pages":"180-195"},"PeriodicalIF":3.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10203226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Oral microbiome diversity: The curious case of Corynebacterium sp. isolation. 口腔微生物组多样性：棒状杆菌分离的奇怪案例。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-10-01 Epub Date: 2022-08-01 DOI: 10.1111/omi.12381

Puthayalai Treerat, Brian McGuire, Elizabeth Palmer, Erin M Dahl, Lisa Karstens, Justin Merritt, Jens Kreth

{"title":"Oral microbiome diversity: The curious case of Corynebacterium sp. isolation.","authors":"Puthayalai Treerat, Brian McGuire, Elizabeth Palmer, Erin M Dahl, Lisa Karstens, Justin Merritt, Jens Kreth","doi":"10.1111/omi.12381","DOIUrl":"10.1111/omi.12381","url":null,"abstract":"Oral microbiome sequencing efforts revealed the presence of hundreds of different microbes. Interindividual differences at strain and species resolution suggest that microbiome diversity could lead to mechanistically distinct gene regulation as well as species-related differences in phenotypes. Commonly, gene regulation and related phenotypes are studied in a few selected strains of a particular species with conclusions that are mostly generalized. The aim of this study was to isolate several species of Corynebacterium using an established protocol that led to the previous isolation of C. durum. Characterization of C. durum interspecies interactions revealed a specific mechanism for chain elongation in Streptococcus sanguinis that was the result of corynebacterial fatty acid production and secretion. While the protocol was successfully applied to isolate what we presumed to be additional Corynebacterium based on several phenotypic traits that seem to be identical to C. durum, genome sequencing of the newly isolated strains placed them closer to Actinomyces. Both Corynebacterium and Actinomyces are suborders of the Actinobacteridae and related species. Our study suggests to take several comprehensive strategies into consideration when taxonomically identifying closely related microorganisms. Furthermore, it seems to be important to test common core phenotypes in bacterial ecology to understand the behavior of specific groups of microbes, rather than simply relying upon genome sequence homology to establish relationships in the microbiome.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"37 5","pages":"167-179"},"PeriodicalIF":3.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578355/pdf/nihms-1825295.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9710686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Fluorescence lectin binding analysis of carbohydrate components in dental biofilms grown in situ in the presence or absence of sucrose. 在存在或不存在蔗糖的情况下原位生长的牙生物膜中碳水化合物成分的荧光凝集素结合分析。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-10-01 DOI: 10.1111/omi.12384

Irene Dige, Pune N Paqué, Yumi Chokyu Del Rey, Marie Braad Lund, Andreas Schramm, Sebastian Schlafer

{"title":"Fluorescence lectin binding analysis of carbohydrate components in dental biofilms grown in situ in the presence or absence of sucrose.","authors":"Irene Dige, Pune N Paqué, Yumi Chokyu Del Rey, Marie Braad Lund, Andreas Schramm, Sebastian Schlafer","doi":"10.1111/omi.12384","DOIUrl":"https://doi.org/10.1111/omi.12384","url":null,"abstract":"Carbohydrate components, such as glycoconjugates and polysaccharides, are constituents of the dental biofilm matrix that play an important role in biofilm stability and virulence. Exopolysaccharides in Streptococcus mutans biofilms have been characterized extensively, but comparably little is known about the matrix carbohydrates in complex, in situ-grown dental biofilms. The present study employed fluorescence lectin binding analysis (FLBA) to investigate the abundance and spatial distribution of glycoconjugates/polysaccharides in biofilms (n = 306) from 10 participants, grown in situ with (SUC) and without (H2O) exposure to sucrose. Biofilms were stained with 10 fluorescently labeled lectins with different carbohydrate specificities (AAL, ABA, ASA, HPA, LEA, MNA-G, MPA, PSA, VGA and WGA) and analyzed by confocal microscopy and digital image analysis. Microbial composition was determined by 16S rRNA gene sequencing. With the exception of ABA, all lectins targeted considerable matrix biovolumes, ranging from 19.3% to 194.0% of the microbial biovolume in the biofilms, which illustrates a remarkable variety of carbohydrate compounds in in situ-grown dental biofilms. MNA-G, AAL, and ASA, specific for galactose, fucose, and mannose, respectively, stained the largest biovolumes. AAL and ASA biovolumes were increased in SUC biofilms, but the difference was not significant due to considerable biological variation. SUC biofilms were enriched in streptococci and showed reduced abundances of Neisseria and Haemophilus spp., but no significant correlations between lectin-stained biovolumes and bacterial abundance were observed. In conclusion, FLBA demonstrates the presence of a voluminous biofilm matrix comprising a variety of different carbohydrate components in complex, in situ-grown dental biofilms.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"37 5","pages":"196-205"},"PeriodicalIF":3.7,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/31/79/OMI-37-196.PMC9804345.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10464295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cover Image, Volume 37, Issue 5 封面图片，第37卷，第5期

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-09-28 DOI: 10.1111/omi.12392

引用次数: 0

Cover Image, Volume 37, Issue 5 封面图片，第37卷，第5期

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-09-28 DOI: 10.1111/omi.12393

Marion Arce, Natalia Endo, Nicolas Dutzan, Loreto Abusleme

引用次数: 0

A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1. 一种新型甘露糖唾液蛋白粘附素参与白色念珠菌细胞与DMBT1的结合。

IF 3.7 3区医学

Molecular Oral Microbiology Pub Date : 2022-06-08 DOI: 10.1111/omi.12374

D. Setoguchi, E. Nagata, T. Oho

{"title":"A novel mannose-containing sialoprotein adhesin involved in the binding of Candida albicans cells to DMBT1.","authors":"D. Setoguchi, E. Nagata, T. Oho","doi":"10.1111/omi.12374","DOIUrl":"https://doi.org/10.1111/omi.12374","url":null,"abstract":"Candida albicans colonizes the oral cavity and causes oral candidiasis and early childhood caries synergistically with cariogenic Streptococcus mutans. Colonization of oral tissues with C. albicans is an essential step in the initiation of these infectious diseases. DMBT1 (deleted in malignant brain tumors 1), also known as salivary agglutinin or gp-340, belongs to the scavenger receptor cysteine-rich (SRCR) superfamily and has important functions in innate immunity. In the oral cavity, DMBT1 causes microbial adherence to tooth enamel and oral mucosa surfaces, but the adherence of C. albicans to DMBT1 has not been examined. In this study, we investigated the binding of C. albicans to DMBT1 and isolated the fungal components responsible for the binding. C. albicans specifically bound to DMBT1 and strongly bound to the peptide domain SRCRP2. Binding to SRCRP2 was inhibited by N-acetylneuraminic acid and mannose and by lectins recognizing these sugars. The isolated component had a molecular mass of 25 kDa, contained sialic acid and mannose residues, and inhibited C. albicans binding to SRCRP2. The localization of the 25-kDa protein on the surface of C. albicans cell walls was confirmed by immunostaining and a cell ELISA using an antiserum to the protein, and Western blotting revealed the presence of the 25-kDa protein in the cell wall fraction of C. albicans. These results suggest that the isolated adhesin is localized on the surface of C. albicans cell walls and that sialic acid and mannose residues in the adhesin play a significant role in the binding reaction. This article is protected by copyright. All rights reserved.","PeriodicalId":18815,"journal":{"name":"Molecular Oral Microbiology","volume":"1 1","pages":""},"PeriodicalIF":3.7,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43086684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1