Molecular plant pathology最新文献_第8页

Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant. 基于转化的基因沉默和 ISC 效应子的功能表征揭示了白粉病真菌如何干扰水杨酸的生物合成和植物的免疫反应。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70030

Jinyao Yin, Xiao Li, Linpeng Dong, Xuehuan Zhu, Yalong Chen, Wenyuan Zhao, Yuhan Liu, Jiaxin Shan, Wenbo Liu, Chunhua Lin, Weiguo Miao

{"title":"Transformation-based gene silencing and functional characterization of an ISC effector reveal how a powdery mildew fungus disturbs salicylic acid biosynthesis and immune response in the plant.","authors":"Jinyao Yin, Xiao Li, Linpeng Dong, Xuehuan Zhu, Yalong Chen, Wenyuan Zhao, Yuhan Liu, Jiaxin Shan, Wenbo Liu, Chunhua Lin, Weiguo Miao","doi":"10.1111/mpp.70030","DOIUrl":"10.1111/mpp.70030","url":null,"abstract":"Obligate biotrophic powdery mildew fungi infect a wide range of economically important plants. These fungi often deliver effector proteins into the host tissues to suppress plant immunity and sustain infection. The phytohormone salicylic acid (SA) is one of the most important signals that activate plant immunity against pathogens. However, how powdery mildew effectors interact with host SA signalling is poorly understood. Isochorismatase (ISC) effectors from two other filamentous pathogens have been found to inhibit host SA biosynthesis by hydrolysing isochorismate, the main SA precursor in the plant cytosol. Here, we identified an ISC effector, named EqIsc1, from the rubber tree powdery mildew fungus Erysiphe quercicola. In ISC enzyme assays, EqIsc1 displayed ISC activity by transferring isochorismate to 2,3-dihydro-2,3-dihydroxybenzoate in vitro and in transgenic Nicotiana benthamiana plants. In EqIsc1-expressing transgenic Arabidopsis thaliana, SA biosynthesis and SA-mediated immune response were significantly inhibited. In addition, we developed an electroporation-mediated transformation method for the genetic manipulation of E. quercicola. Inoculation of rubber tree leaves with EqIsc1-silenced E. quercicola strain induced SA-mediated immunity. We also detected the translocation of EqIsc1 into the plant cytosol during the interaction between E. quercicola and its host. Taken together, our results suggest that a powdery mildew effector functions as an ISC enzyme to hydrolyse isochorismate in the host cytosol, altering the SA biosynthesis and immune response.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70030"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Naturally Occurring Amino Acid Substitution in the VPg α1-α2 Loop Breaks eIF4E-Mediated Resistance to PRSV by Enabling VPg to Re-Hijack Another eIF4E Isoform eIF(iso)4E in Watermelon. VPg α1-α2环路中天然存在的氨基酸取代使VPg能够重新劫持西瓜中的另一种eIF4E异构体eIF(iso)4E，从而打破了eIF4E介导的对PRSV的抗性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70033

Ling-Xi Zhou, Xiao Yin, Zhi-Yong Yan, Jun Jiang, Yan-Ping Tian, Rui Gao, Chao Geng, Xiang-Dong Li

{"title":"The Naturally Occurring Amino Acid Substitution in the VPg α1-α2 Loop Breaks eIF4E-Mediated Resistance to PRSV by Enabling VPg to Re-Hijack Another eIF4E Isoform eIF(iso)4E in Watermelon.","authors":"Ling-Xi Zhou, Xiao Yin, Zhi-Yong Yan, Jun Jiang, Yan-Ping Tian, Rui Gao, Chao Geng, Xiang-Dong Li","doi":"10.1111/mpp.70033","DOIUrl":"10.1111/mpp.70033","url":null,"abstract":"Plant resistance, which acts as a selective pressure that affects viral population fitness, leads to the emergence of resistance-breaking virus strains. Most recessive resistance to potyviruses is related to the mutation of eukaryotic translation initiation factor 4E (eIF4E) or its isoforms that break their interactions with the viral genome-linked protein (VPg). In this study, we found that the VPg α1-α2 loop, which is essential for binding eIF4E, is the most variable domain of papaya ringspot virus (PRSV) VPg. PRSV VPg with the naturally occurring amino acid substitution of K105Q or E108G in the α1-α2 loop fails to interact with watermelon (Citrullus lanatus) eIF4E but interacts with watermelon eIF(iso)4E instead. Moreover, PRSV carrying these mutations can break the eIF4E-mediated resistance to PRSV in watermelon accession PI 244019. We further revealed that watermelon eIF(iso)4E with the amino acid substitutions of DNQS to GAAA in the cap-binding pocket could not interact with PRSV VPg with natural amino acid substitution of K105Q or E108G. Therefore, our finding provides a precise target for engineering watermelon germplasm resistant to resistance-breaking PRSV isolates.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70033"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flg22-facilitated PGPR colonization in root tips and control of root rot. Flg22 促进 PGPR 在根尖定殖并控制根腐病。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70026

Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu

{"title":"Flg22-facilitated PGPR colonization in root tips and control of root rot.","authors":"Yanan Li, Yafei Li, Yuepeng Wang, Yanqing Yang, Man Qi, Tongfu Su, Rui Li, Dehai Liu, Yuqian Gao, Yuancheng Qi, Liyou Qiu","doi":"10.1111/mpp.70026","DOIUrl":"10.1111/mpp.70026","url":null,"abstract":"Plant root border cells (RBCs) prevent the colonization of plant growth-promoting rhizobacteria (PGPR) at the root tip, rendering the PGPR unable to effectively control pathogens infecting the root tip. In this study, we engineered four strains of Pseudomonas sp. UW4, a typical PGPR strain, each carrying an enhanced green fluorescent protein (EGFP)-expressing plasmid. The UW4E strain harboured only the plasmid, whereas the UW4E-flg22 strain expressed a secreted EGFP-Flg22 fusion protein, the UW4E-Flg(flg22) strain expressed a non-secreted Flg22, and the UW4E-flg22-D strain expressed a secreted Flg22-DNase fusion protein. UW4E-flg22 and UW4E-flg22-D, which secreted Flg22, induced an immune response in wheat RBCs and colonized wheat root tips, whereas the other strains, which did not secrete Flg22, failed to elicit this response and did not colonize wheat root tips. The immune response revealed that wheat RBCs synthesized mucilage, extracellular DNA, and reactive oxygen species. Furthermore, the Flg22-secreting strains showed a 33.8%-93.8% higher colonization of wheat root tips and reduced the root rot incidence caused by Rhizoctonia solani and Fusarium pseudograminearum by 24.6%-35.7% compared to the non-Flg22-secreting strains in pot trials. There was a negative correlation between the incidence of wheat root rot and colonization of wheat root tips by these strains. In contrast, wheat root length and dry weight were positively correlated with the colonization of wheat root tips by these strains. These results demonstrate that engineered secretion of Flg22 by PGPR is an effective strategy for controlling root rot and improving plant growth.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70026"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis. 作为杀菌剂的除草剂：以玉米病原体 Ustilago maydis 的血红素生物合成为目标。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70007

Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad

{"title":"Herbicides as fungicides: Targeting heme biosynthesis in the maize pathogen Ustilago maydis.","authors":"Djihane Damoo, Matthias Kretschmer, Christopher W J Lee, Cornelia Herrfurth, Ivo Feussner, Kai Heimel, James W Kronstad","doi":"10.1111/mpp.70007","DOIUrl":"10.1111/mpp.70007","url":null,"abstract":"Pathogens must efficiently acquire nutrients from host tissue to proliferate, and strategies to block pathogen access therefore hold promise for disease control. In this study, we investigated whether heme biosynthesis is an effective target for ablating the virulence of the phytopathogenic fungus Ustilago maydis on maize plants. We first constructed conditional heme auxotrophs of the fungus by placing the heme biosynthesis gene hem12 encoding uroporphyrinogen decarboxylase (Urod) under the control of nitrogen or carbon source-regulated promoters. These strains were heme auxotrophs under non-permissive conditions and unable to cause disease in maize seedlings, thus demonstrating the inability of the fungus to acquire sufficient heme from host tissue to support proliferation. Subsequent experiments characterized the role of endocytosis in heme uptake, the susceptibility of the fungus to heme toxicity as well as the transcriptional response to exogenous heme. The latter RNA-seq experiments identified a candidate ABC transporter with a role in the response to heme and xenobiotics. Given the importance of heme biosynthesis for U. maydis pathogenesis, we tested the ability of the well-characterized herbicide BroadStar to influence disease. This herbicide contains the active ingredient flumioxazin, an inhibitor of Hem14 in the heme biosynthesis pathway, and we found that it was an effective antifungal agent for blocking disease in maize. Thus, repurposing herbicides for which resistant plants are available may be an effective strategy to control pathogens and achieve crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70007"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11530707/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection. 黄瓜绿斑驳病毒外壳蛋白劫持线粒体 ATPδ 促进病毒感染

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70034

Xue Yang, Xing-Lin Jiang, Han Fu, Lian-Wei Yu, Niu Ai, Ya-Juan Shi, Yu-Wen Lu, Zi-Hao Xia, Hong-Lian Li, Yan Shi

{"title":"Cucumber Green Mottle Mosaic Virus Coat Protein Hijacks Mitochondrial ATPδ to Promote Viral Infection.","authors":"Xue Yang, Xing-Lin Jiang, Han Fu, Lian-Wei Yu, Niu Ai, Ya-Juan Shi, Yu-Wen Lu, Zi-Hao Xia, Hong-Lian Li, Yan Shi","doi":"10.1111/mpp.70034","DOIUrl":"10.1111/mpp.70034","url":null,"abstract":"The production and scavenging of reactive oxygen species (ROS) are critical for plants to adapt to biotic and abiotic stresses. In this study, we investigated the interaction between the coat protein (CP) of cucumber green mottle mosaic virus (CGMMV) and ATP synthase subunit δ (ATPδ) in mitochondria. Silencing of ATPδ by tobacco rattle virus-based virus-induced gene silencing impeded CGMMV accumulation in Nicotiana benthamiana leaves. Both the overexpression of ATPδ in transgenic plants and transient expression promoted CGMMV infection. Nitro blue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB) staining revealed that ATPδ inhibited O2 - production but not H2O2 production. The treatment of CGMMV-infected leaves with the ROS inhibitor diphenylene iodonium (DPI) induced a ROS burst that inhibited CGMMV infection. Reverse transcription-quantitative PCR and superoxide dismutase (SOD) activity assays showed that ATPδ, CGMMV infection, and CP expression specifically induced NbFeSOD3/4 expression and SOD activity, and silencing NbFeSOD3/4 inhibited CGMMV infection. We speculate that CGMMV CP interacts with ATPδ and hijacks it, thereby enhancing O2 - quenching by upregulating NbFeSOD expression and, in turn, SOD activity.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70034"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11588859/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142716570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TOR signalling pathway in fungal phytopathogens: A target for plant disease control. 真菌植物病原体中的 TOR 信号通路：植物病害控制的目标

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70024

Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren

{"title":"The TOR signalling pathway in fungal phytopathogens: A target for plant disease control.","authors":"Yun Song, Yaru Wang, Huafang Zhang, Muhammad Abu Bakar Saddique, Xiumei Luo, Maozhi Ren","doi":"10.1111/mpp.70024","DOIUrl":"10.1111/mpp.70024","url":null,"abstract":"Plant diseases caused by fungal phytopathogens have led to significant economic losses in agriculture worldwide. The management of fungal diseases is mainly dependent on the application of fungicides, which are not suitable for sustainable agriculture, human health, and environmental safety. Thus, it is necessary to develop novel targets and green strategies to mitigate the losses caused by these pathogens. The target of rapamycin (TOR) complexes and key components of the TOR signalling pathway are evolutionally conserved in pathogens and closely related to the vegetative growth and pathogenicity. As indicated in recent systems, chemical, genetic, and genomic studies on the TOR signalling pathway, phytopathogens with TOR dysfunctions show severe growth defects and nonpathogenicity, which makes the TOR signalling pathway to be developed into an ideal candidate target for controlling plant disease. In this review, we comprehensively discuss the current knowledge on components of the TOR signalling pathway in microorganisms and the diverse roles of various plant TOR in response to plant pathogens. Furthermore, we analyse a range of disease management strategies that rely on the TOR signalling pathway, including genetic modification technologies and chemical controls. In the future, disease control strategies based on the TOR signalling network are expected to become a highly effective weapon for crop protection.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70024"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541241/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance. 一种来自 Ciboria shiraiana 的新型蛋白诱导剂（Cs08297）可增强植物的抗病性。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70023

Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao

{"title":"A novel protein elicitor (Cs08297) from Ciboria shiraiana enhances plant disease resistance.","authors":"Shuai Zhang, Ruolan Li, Wei Fan, Xuefei Chen, Caiquan Tao, Shuman Liu, Panpan Zhu, Shuchang Wang, Aichun Zhao","doi":"10.1111/mpp.70023","DOIUrl":"10.1111/mpp.70023","url":null,"abstract":"Ciboria shiraiana is a necrotrophic fungus that causes mulberry sclerotinia disease resulting in huge economic losses in agriculture. During infection, the fungus uses immunity elicitors to induce plant tissue necrosis that could facilitate its colonization on plants. However, the key elicitors and immune mechanisms remain unclear in C. shiraiana. Herein, a novel elicitor Cs08297 secreted by C. shiraiana was identified, and it was found to target the apoplast in plants to induce cell death. Cs08297 is a cysteine-rich protein unique to C. shiraiana, and cysteine residues in Cs08297 were crucial for its ability to induce cell death. Cs08297 induced a series of defence responses in Nicotiana benthamiana, including the burst of reactive oxygen species (ROS), callose deposition, and activation of defence-related genes. Cs08297 induced-cell death was mediated by leucine-rich repeat (LRR) receptor-like kinases BAK1 and SOBIR1. Purified His-tagged Cs08297-thioredoxin fusion protein triggered cell death in different plants and enhanced plant resistance to diseases. Cs08297 was necessary for sclerotial development, oxidative-stress adaptation, and cell wall integrity but negatively regulated virulence of C. shiraiana. In conclusion, our results revealed that Cs08297 is a novel fungal elicitor in fungi inducing plant immunity. Furthermore, its potential to enhance plant resistance provides a new target to control agricultural diseases biologically.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70023"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11534627/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142575675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Ny_tbr. 单个可磷酸化氨基酸残基对于马铃薯 Nytbr 识别多种壶状病毒 HCPro 效应体至关重要。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70027

Bryce G Alex, Zong-Ying Zhang, Danny Lasky, Hernan Garcia-Ruiz, Ronnie Dewberry, Caitilyn Allen, Dennis Halterman, Aurélie M Rakotondrafara

{"title":"A single phosphorylatable amino acid residue is essential for the recognition of multiple potyviral HCPro effectors by potato Nytbr.","authors":"Bryce G Alex, Zong-Ying Zhang, Danny Lasky, Hernan Garcia-Ruiz, Ronnie Dewberry, Caitilyn Allen, Dennis Halterman, Aurélie M Rakotondrafara","doi":"10.1111/mpp.70027","DOIUrl":"10.1111/mpp.70027","url":null,"abstract":"Potato virus Y (PVY, Potyviridae) is among the most important viral pathogens of potato. The potato resistance gene Nytbr confers hypersensitive resistance to the ordinary strain of PVY (PVYO), but not the necrotic strain (PVYN). Here, we unveil that residue 247 of PVY helper component proteinase (HCPro) acts as a central player controlling Nytbr strain-specific activation. We found that substituting the serine at 247 in the HCPro of PVYO (HCProO) with an alanine as in PVYN HCPro (HCProN) disrupts Nytbr recognition. Conversely, an HCProN mutant carrying a serine at position 247 triggers defence. Moreover, we demonstrate that plant defences are induced against HCProO mutants with a phosphomimetic or another phosphorylatable residue at 247, but not with a phosphoablative residue, suggesting that phosphorylation could modulate Nytbr resistance. Extending beyond PVY, we establish that the same response elicited by the PVYO HCPro is also induced by HCPro proteins from other members of the Potyviridae family that have a serine at position 247, but not by those with an alanine. Together, our results provide further insights in the strain-specific PVY resistance in potato and infer a broad-spectrum detection mechanism of plant potyvirus effectors contingent on a single amino acid residue.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70027"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11541239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142591341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of zju-miR156c-mediated network in regulating witches' broom symptom of Chinese jujube. zju-miR156c介导的网络调控大枣 "巫婆帚 "症状的机制

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-11-01 DOI: 10.1111/mpp.70031

Yunjie Wang, Zhi Luo, Xuan Zhao, Hongqiang Sun, Jiaxin Liu, Dongfeng Zhang, Haonan Cao, Changfeng Ai, Lihu Wang, Li Dai, Mengjun Liu, Lixin Wang, Zhiguo Liu

{"title":"Mechanism of zju-miR156c-mediated network in regulating witches' broom symptom of Chinese jujube.","authors":"Yunjie Wang, Zhi Luo, Xuan Zhao, Hongqiang Sun, Jiaxin Liu, Dongfeng Zhang, Haonan Cao, Changfeng Ai, Lihu Wang, Li Dai, Mengjun Liu, Lixin Wang, Zhiguo Liu","doi":"10.1111/mpp.70031","DOIUrl":"10.1111/mpp.70031","url":null,"abstract":"Jujube witches' broom, caused by phytoplasma, is a destructive disease of Chinese jujube. Studies have shown that zju-miR156s play an important role in phytoplasma infection in jujube, but the regulatory mechanism between zju-miR156c and witches' broom remains unexplored. In the current study, miRNA-seq and gene expression analysis showed that zju-miR156c was more highly induced in infected jujube plants than the other miRNAs and its target gene was ZjSPL3. In addition, the expression levels of thymidylate kinase gene (TMKJWB) and secreted jujube protein (SJP1JWB) in diseased materials were higher than those in healthy controls. The expression level of zju-miR156c was significantly upregulated, while ZjSPL3 was sharply downregulated and the content of cytokinin (CTK) significantly increased. Overexpression of zju-miR156c in Arabidopsis significantly reduced the expression of AtSPL10 (homologous gene of ZjSPL3) but increased the content of CTK, and the transgenic plants exhibited witches' broom symptoms. In addition, yeast two-hybrid and co-immunoprecipitation assays confirmed that SJP1JWB interacted with ZjERF18. Yeast one-hybrid analysis showed that ZjERF18 could interact with the promoter of zju-MIR156c. In conclusion, our results demonstrated a novel pathogenic module of ZjERF18-zju-miR156c-ZjSPL3-CTK has an important function in the formation of witches' broom caused by SJP1JWB.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 11","pages":"e70031"},"PeriodicalIF":4.8,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11573724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142667823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing RNA interference for the control of Fusarium species: A critical review. 利用 RNA 干扰来控制镰刀菌：重要综述。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-10-01 DOI: 10.1111/mpp.70011

Caihong Liu, Karl-Heinz Kogel, Maria Ladera-Carmona

{"title":"Harnessing RNA interference for the control of Fusarium species: A critical review.","authors":"Caihong Liu, Karl-Heinz Kogel, Maria Ladera-Carmona","doi":"10.1111/mpp.70011","DOIUrl":"10.1111/mpp.70011","url":null,"abstract":"Fusarium fungi are a pervasive threat to global agricultural productivity. They cause a spectrum of plant diseases that result in significant yield losses and threaten food safety by producing mycotoxins that are harmful to human and animal health. In recent years, the exploitation of the RNA interference (RNAi) mechanism has emerged as a promising avenue for the control of Fusarium-induced diseases, providing both a mechanistic understanding of Fusarium gene function and a potential strategy for environmentally sustainable disease management. However, despite significant progress in elucidating the presence and function of the RNAi pathway in different Fusarium species, a comprehensive understanding of its individual protein components and underlying silencing mechanisms remains elusive. Accordingly, while a considerable number of RNAi-based approaches to Fusarium control have been developed and many reports of RNAi applications in Fusarium control under laboratory conditions have been published, the applicability of this knowledge in agronomic settings remains an open question, and few convincing data on RNAi-based disease control under field conditions have been published. This review aims to consolidate the current knowledge on the role of RNAi in Fusarium disease control by evaluating current research and highlighting important avenues for future investigation.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 10","pages":"e70011"},"PeriodicalIF":4.8,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11450251/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142372250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0