Molecular plant pathology最新文献_第8页

Cotton RLP6 Interacts With NDR1/HIN6 to Enhance Verticillium Wilt Resistance via Altering ROS and SA. 棉花RLP6与NDR1/HIN6互作通过改变ROS和SA增强黄萎病抗性

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2025-01-01 DOI: 10.1111/mpp.70052

Dongmei Zhang, Yan Wang, Qishen Gu, Lixia Liu, Zhicheng Wang, Jin Zhang, Chengsheng Meng, Jun Yang, Zixu Zhang, Zhiying Ma, Xingfen Wang, Yan Zhang

{"title":"Cotton RLP6 Interacts With NDR1/HIN6 to Enhance Verticillium Wilt Resistance via Altering ROS and SA.","authors":"Dongmei Zhang, Yan Wang, Qishen Gu, Lixia Liu, Zhicheng Wang, Jin Zhang, Chengsheng Meng, Jun Yang, Zixu Zhang, Zhiying Ma, Xingfen Wang, Yan Zhang","doi":"10.1111/mpp.70052","DOIUrl":"10.1111/mpp.70052","url":null,"abstract":"Cotton Verticillium wilt (VW) is often a destructive disease that results in significant fibre yield and quality losses in Gossypium hirsutum. Transferring the resistance trait of Gossypium barbadense to G. hirsutum is optional but challenging in traditional breeding due to limited molecular dissections of resistance genes. Here, we discovered a species-diversified structural variation (SV) in the promoter of receptor-like protein 6 (RLP6) that caused distinctly higher expression level of RLP6 in G. barbadense with the SV than G. hirsutum without the SV. Functional experiments showed that RLP6 is an important regulator in mediating VW resistance. Overexpressing RLP6 significantly enhanced resistance and root growth, whereas the opposite phenotype appeared in RLP6-silenced cotton. A series of experiments indicated that RLP6 regulated reactive oxygen species (ROS) and salicylic acid (SA) signalling, which induced diversified defence-related gene expression with pathogenesis-related (PR) proteins and cell wall proteins enrichments for resistance improvement. These findings could be valuable for the transfer of the G. barbadense SV locus to improve G. hirsutum VW resistance in future crop disease resistance breeding.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 1","pages":"e70052"},"PeriodicalIF":4.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11753439/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143024053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ClBeclin1 Positively Regulates Citrus Defence Against Citrus Yellow Vein Clearing Virus Through Mediating Autophagy-Dependent Degradation of ClAPX1. ClBeclin1通过介导ClAPX1自噬依赖性降解正向调节柑橘对柑橘黄脉清除病毒的防御

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70041

Jiajun Wang, Ling Yu, Jinfa Zhao, Shimin Fu, Yalin Mei, Binghai Lou, Yan Zhou

{"title":"ClBeclin1 Positively Regulates Citrus Defence Against Citrus Yellow Vein Clearing Virus Through Mediating Autophagy-Dependent Degradation of ClAPX1.","authors":"Jiajun Wang, Ling Yu, Jinfa Zhao, Shimin Fu, Yalin Mei, Binghai Lou, Yan Zhou","doi":"10.1111/mpp.70041","DOIUrl":"10.1111/mpp.70041","url":null,"abstract":"Autophagy, one of the most widespread and highly conserved protein degradation systems in eukaryotic cells, plays an important role in plant growth, development and stress response. Beclin 1 is a core component of the phosphatidylinositol 3-kinase (PI3K) autophagy complex and positively regulates plant immunity against viruses. The upregulation of Eureka lemon ClBeclin1 was observed in response to citrus yellow vein clearing virus (CYVCV) infection. However, the function of ClBeclin1 and the underlying mechanism during CYVCV colonisation remain unclear. Here, the resistance evaluation of the overexpression and silencing of ClBeclin1 in Eureka lemon hairy roots revealed it as a positive regulator of citrus immunity against CYVCV. Transcriptomic profiling and metabolic analyses along with genetic evidence implied that the overexpression of ClBeclin1 positively triggered reactive oxygen species (ROS)- and jasmonic acid (JA)-mediated immunity in citrus. The accumulation of ROS and JA contents was attributed to the autophagic degradation of the ROS scavenger ClAPX1 via ClBeclin1 overexpression. Exogenous application of either H2O2 or JA significantly reduced CYVCV colonisation and vein-clearing symptoms on the host. Collectively, our findings indicate that ClBeclin1 activation contributes to citrus immunity against CYVCV through triggering ROS- and JA-mediated defence responses, and the accumulation of ROS and JA resulted from the autophagic degradation of ClAPX1 by ClBeclin1.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70041"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631719/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Genomics Reveals Sources of Genetic Variability in the Asexual Fungal Plant Pathogen Colletotrichum lupini. 比较基因组学揭示了无性真菌植物病原体 Colletotrichum lupini 的遗传变异来源。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70039

Joris A Alkemade, Pierre Hohmann, Monika M Messmer, Timothy G Barraclough

{"title":"Comparative Genomics Reveals Sources of Genetic Variability in the Asexual Fungal Plant Pathogen Colletotrichum lupini.","authors":"Joris A Alkemade, Pierre Hohmann, Monika M Messmer, Timothy G Barraclough","doi":"10.1111/mpp.70039","DOIUrl":"10.1111/mpp.70039","url":null,"abstract":"Fungal plant pathogens cause major crop losses worldwide, with many featuring compartmentalised genomes that include both core and accessory regions, which are believed to drive adaptation. The highly host-specific fungus Colletotrichum lupini greatly impacts lupin (Lupinus spp.) cultivation. This pathogen is part of clade 1 of the C. acutatum species complex and comprises four genetically uniform, presumably clonal, lineages (I-IV). Despite this, variation in virulence and morphology has been observed within these lineages. To investigate the potential sources of genetic variability in this asexual fungus, we compared the genomes of 16 C. lupini strains and 17 related Colletotrichum species. Phylogenomics confirmed the presence of four distinct lineages, but further examination based on genome size, gene content, transposable elements (TEs), and deletions revealed that lineage II could be split into two groups, II-A and II-B. TE content varied between lineages and correlated strongly with genome size variation, supporting a role for TEs in genome expansion in this species. Pangenome analysis revealed a highly variable accessory genome, including a minichromosome present in lineages II, III, and IV, but absent in lineage I. Accessory genes and effectors appeared to cluster in proximity to TEs. Presence/absence variation of putative effectors was lineage-specific, suggesting that these genes play a crucial role in determining host range. Notably, no effectors were found on the TE-rich minichromosome. Our findings shed light on the potential mechanisms generating genetic diversity in this asexual fungal pathogen that could aid future disease management.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70039"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane. 含FFAT基序的黄单胞菌III型效应蛋白XopM抑制植物质膜上的MTI反应。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70038

Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke

{"title":"XopM, An FFAT Motif-Containing Type III Effector Protein From Xanthomonas, Suppresses MTI Responses at the Plant Plasma Membrane.","authors":"Charlotte Brinkmann, Jennifer Bortlik, Margot Raffeiner, Manuel González-Fuente, Linus F Börnke, Suayib Üstün, Frederik Börnke","doi":"10.1111/mpp.70038","DOIUrl":"10.1111/mpp.70038","url":null,"abstract":"Many gram-negative pathogenic bacteria use type III effector proteins (T3Es) as essential virulence factors to suppress host immunity and to cause disease. However, in many cases the molecular function of T3Es remains unknown. The plant pathogen Xanthomonas campestris pv. vesicatoria (Xcv) is the causal agent of bacterial spot disease on tomato and pepper plants and is known to translocate around 36 T3Es into its host cell, which collectively suppress plant defence and promote infection. XopM is an Xcv core T3E with unknown function that has no similarity to any other known protein. We found that XopM interacts with vesicle-associated membrane protein (VAMP)-associated proteins (VAPs) in an isoform-specific manner. The endoplasmic reticulum (ER) integral membrane protein VAP is a common component of membrane contact sites involved in both tethering and lipid transfer by binding directly to proteins containing an FFAT (two phenylalanines [FF] in an acidic tract [AT]) motif. Sequence analyses revealed that XopM displays two FFAT motifs that cooperatively mediated the interaction of XopM with VAP. When expressed in plants, XopM supported growth of a nonpathogenic bacterial strain and dampened the production of reactive oxygen species, indicating its ability to suppress plant immunity. Further analyses revealed that the interaction with VAP and the ability to suppress microbe-associated molecular pattern-triggered immunity (MTI) are structurally and functionally separable, although XopM requires localisation to the host membrane system for full MTI suppression activity. We discuss a working model in which XopM uses FFAT motifs to target the membrane to interfere with early MTI responses.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70038"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631713/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ralstonia solanacearum Alters Root Developmental Programmes in Auxin-Dependent and -Independent Manners. 植物生长素依赖性和非依赖性改变根系发育规律。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70043

Lu Zhang, Gang Yu, Hao Xue, Meng Li, Rosa Lozano-Durán, Alberto P Macho

{"title":"Ralstonia solanacearum Alters Root Developmental Programmes in Auxin-Dependent and -Independent Manners.","authors":"Lu Zhang, Gang Yu, Hao Xue, Meng Li, Rosa Lozano-Durán, Alberto P Macho","doi":"10.1111/mpp.70043","DOIUrl":"10.1111/mpp.70043","url":null,"abstract":"Microbial pathogens and other parasites can modify the development of their hosts, either as a target or a side effect of their virulence activities. The plant-pathogenic bacterium Ralstonia solanacearum, causal agent of the devastating bacterial wilt disease, is a soilborne microbe that invades host plants through their roots and later proliferates in xylem vessels. In this work, we studied the early stages of R. solanacearum infection in the model plant Arabidopsis thaliana, using an in vitro infection system. In addition to the previously reported inhibition of primary root length and increase in root hair formation at the root tip, we observed an earlier xylem differentiation during R. solanacearum infection that occurs in a HrpG-dependent manner, suggesting that the pathogen actively promotes the development of the vascular system upon invasion of the root. Moreover, we found that the phytohormone auxin, of which the accumulation is promoted by the bacterial infection, is required for the R. solanacearum-triggered induction of root hair formation but not earlier xylem differentiation. Altogether, our results shed light on the capacity of R. solanacearum to induce alterations of root developmental pathways and on the role of auxin in this process.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70043"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11662138/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142872704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae. 大豆疫霉卵菌侵染植物器官的特异性转录调控。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70042

Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang

{"title":"Specific Transcriptional Regulation Controls Plant Organ-Specific Infection by the Oomycete Pathogen Phytophthora sojae.","authors":"Long Lin, Yang Wang, Hui Qian, Jiawei Wu, Yachun Lin, Yeqiang Xia, Suomeng Dong, Wenwu Ye, Yuanchao Wang","doi":"10.1111/mpp.70042","DOIUrl":"10.1111/mpp.70042","url":null,"abstract":"The organs of a plant species vary in cell structure, metabolism and defence responses. However, the mechanisms that enable a single pathogen to colonise different plant organs remain unclear. Here we compared the transcriptome of the oomycete pathogen Phytophthora sojae during infection of roots versus leaves of soybeans. We found differences in the transcript levels of hundreds of pathogenicity-related genes, particularly genes encoding carbohydrate-active enzymes, secreted (effector) proteins, oxidoreductase-related proteins and transporters. To identify the key regulator for root-specific infection, we knocked out root-specific transcription factors (TFs) and found the mutants of PsBZPc29, which encodes a member of an oomycete-specific class of basic leucine zipper (bZIP) TFs, displayed reduced virulence on soybean roots but not on leaves. More than 60% of the root-specific genes showed reduced expression in the mutants during root infection. The results suggest that transcriptional regulation underlies the organ-specific infection by P. sojae, and that a bZIP TF plays a key role in root-specific transcriptional regulation.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70042"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11645254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142822127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. 更正：新的持久性植物RNA病毒携带突变以削弱抗病毒RNA干扰的病毒抑制。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70035

{"title":"Correction to: New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference.","authors":"","doi":"10.1111/mpp.70035","DOIUrl":"10.1111/mpp.70035","url":null,"abstract":"Zhu, L.-J., Zhu, Y., Zou, C., Su, L.-Y., Zhang, C.-T., Wang, C. et al. (2024) New persistent plant RNA virus carries mutations to weaken viral suppression of antiviral RNA interference. Molecular Plant Pathology, 25, e70020. Available from: https://doi.org/10.1111/mpp.70020 The following errors have been identified in the above published article: The co-first authors and co-corresponding authors are not indicated. The order of the funds appearing in the Funding information of the article are not consistent with the order in the Acknowledgements. The authors would like to correct these errors as follows: Li-Juan Zhu and Yu Zhu contributed equally to this work. Jian-Guo Wu and Yan-Hong Han are co-corresponding authors. Jiang-Guo Wu: wujianguo81@126.com; Yan-Hong Han: yan-hong@fafu.edu The correct order of funds is: National Natural Science Foundation of China, Grant/Award Number 32025031 and 31,900,153, National Key Research and Development Program of China, Grant/Award Number 2023YFF1000500 and Special Fund Project for Science and Technology Innovation of FAFU, Grant/Award Number KFB23013. We apologise for these errors.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70035"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11631711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate. DNA连接酶I在生物分子凝聚物中使马铃薯纺锤形块茎类病毒RNA环状化。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70047

Yunhan Wang, Junfei Ma, Jie Hao, Bin Liu, Ying Wang

{"title":"DNA Ligase I Circularises Potato Spindle Tuber Viroid RNA in a Biomolecular Condensate.","authors":"Yunhan Wang, Junfei Ma, Jie Hao, Bin Liu, Ying Wang","doi":"10.1111/mpp.70047","DOIUrl":"10.1111/mpp.70047","url":null,"abstract":"Viroids are single-stranded circular noncoding RNAs that mainly infect crops. Upon infection, nuclear-replicating viroids engage host DNA-dependent RNA polymerase II for RNA-templated transcription, which is facilitated by a host protein TFIIIA-7ZF. The sense-strand and minus-strand RNA intermediates are differentially localised to the nucleolus and nucleoplasm regions, respectively. The factors and function underlying the differential localisation of viroid RNAs have not been fully elucidated. The sense-strand RNA intermediates are cleaved into linear monomers by a yet-to-be-identified RNase III-type enzyme and ligated to form circular RNA progeny by DNA ligase I (LIG1). The subcellular compartment for the ligation reaction has not been characterised. Here, we show that LIG1 and potato spindle tuber viroid (PSTVd) colocalise near the nucleolar region in Nicotiana benthamiana protoplasts. The colocalised region is also the highly condensed region of sense-strand PSTVd RNA, indicating that PSTVd RNA and LIG1 form a biomolecular condensate for RNA processing. This finding expands the function of biomolecular condensates to the infection of subviral pathogens. In addition, this knowledge of viroid biogenesis will contribute to exploring thousands of viroid-like RNAs that have been recently identified.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70047"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11665776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142882447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to 'Heat Shock Transcription Factor 3 Regulates Plant Immune Response Through Modulation of Salicylic Acid Accumulation and Signalling in Cassava'. 更正“热休克转录因子3通过调节木薯水杨酸积累和信号传导调节植物免疫反应”。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70032

引用次数: 0

Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies. Lrp家族调节因子SCAB_Lrp2响应前体色氨酸并抑制疥疮链霉菌的Thaxtomin生物合成。

IF 4.8 1区农林科学

Molecular plant pathology Pub Date : 2024-12-01 DOI: 10.1111/mpp.70036

Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu

{"title":"Lrp Family Regulator SCAB_Lrp2 Responds to the Precursor Tryptophan and Represses the Thaxtomin Biosynthesis in Streptomyces scabies.","authors":"Haoyang He, Lijuan Tang, Mingrui Song, Hui Chen, Youquan Zou, Xueyan Li, Endong Yang, Hang Wu, Buchang Zhang, Jing Liu","doi":"10.1111/mpp.70036","DOIUrl":"https://doi.org/10.1111/mpp.70036","url":null,"abstract":"Streptomyces scabies is a well-researched plant pathogen belonging to the genus Streptomyces. Its virulence is linked to the production of the secondary metabolite thaxtomin A, which is tightly regulated at the transcriptional level. The leucine-responsive regulatory protein (Lrp) family is conserved in prokaryotes and is involved in various crucial biological processes. However, the regulatory interaction between Lrp protein and pathogenic Streptomyces species remains poorly understood. This study aims to explore the role of SCAB_Lrp2 in regulating thaxtomin biosynthesis and pathogenicity, and to analyse the shared and unique features of Lrp homologues in S. scabies. We observed that SCAB_Lrp2 (SCAB_75421) showed significant homology with SCAB_Lrp, a recognised activator of thaxtomin A production in S. scabies. Our results revealed a regulatory interaction between SCAB_Lrp2 and SCAB_Lrp in terms of their targets, although SCAB_Lrp2 does not respond to the amino acid-effectors of SCAB_Lrp. In contrast to SCAB_Lrp, deletion of SCAB_Lrp2 resulted in a notable increase in thaxtomin A production with the emergence of a hypervirulent phenotype in S. scabies. Further analysis revealed that SCAB_Lrp2 represses the transcription of the thaxtomin biosynthetic gene cluster by directly regulating the cluster-situated regulator (CSR) gene txtR. Moreover, the precursor of thaxtomin, tryptophan, acts as an effector of SCAB_Lrp2, strengthening the repressive effect on thaxtomin biosynthesis through txtR. These findings provide new insights into the functional conservation and diversity of Lrp homologues involved in the biosynthesis of thaxtomin phytotoxins in pathogenic Streptomyces species.","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"25 12","pages":"e70036"},"PeriodicalIF":4.8,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11609053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142770581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0