Molecular Biology Reports最新文献

{"title":"Alterations of Alpl and bFGF levels in peripheral tissues after mild traumatic brain injury: implications on sexual differences.","authors":"Su Mercan Gülsu, Tuba Dilay Kökenek Ünal, Zeynep Yılmaz Şükranlı, Ecmel Mehmetbeyoğlu Duman, Samed Yora, Mehmet Memiş, Serpil Taheri","doi":"10.1007/s11033-025-10599-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10599-9","url":null,"abstract":"<p><strong>Background: </strong>Mild traumatic brain injury (mTBI) is a significant health issue affecting people of all ages and socioeconomic groups. Its clinical spectrum alters from transient mild symptoms to disabling problems. The molecular mechanisms that affect the vital functions and treatment after mTBI have yet to be fully understood. This study aims to investigate the expression levels of Alkaline Phosphatase (Alpl) and Fibroblast Growth Factor (bFGF) in peripheral organs after mTBI in traumatic mice models and observe the differences between acute and chronic phases.</p><p><strong>Methods and results: </strong>A total of 30 animals were randomly divided into three groups, with equal numbers of males and females in each group. mTBI was induced in mice utilizing the Marmarau trauma model. Alpl and bFGF expression levels in the acute and chronic phases were determined via Real-Time PCR in liver, kidney, bone, and muscle tissues. Alpl and bFGF gene expressions in the acute phase after mTBI increased significantly. In the kidney, Alpl and bFGF levels increased in the acute phase in females, but bFGF significantly decreased in males. In muscle tissue bFGF levels significantly increased in males in the chronic phase. Our study showed significant differences between sexes in response to mTBI.</p><p><strong>Conclusions: </strong>Our study investigated the role of Alpl and bFGF genes in peripheral tissues in acute and chronic phases after mTBI for the first time in the literature. The data obtained will guide understanding the secondary events and the consequences of the disease in mTBI and taking the necessary treatment and measures.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"505"},"PeriodicalIF":2.6,"publicationDate":"2025-05-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morpho-physio-biochemical and gene expression profiling of drought-tolerant sugarcane genotypes (Saccharum hybrids).","authors":"Ashgan E Abou Gabal, Ahmed E Khalid, Eid M E Mehareb, Asmaa M M Elfaramawy, Asmaa S M Haraz","doi":"10.1007/s11033-025-10592-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10592-2","url":null,"abstract":"<p><strong>Background: </strong>In this study, we assess how well 10 types of sugarcane (Saccharum spp. L.) can handle drought and produce sugar based on water loss from the crop to help with future breeding programs. The experiment entails subjecting the plants at 120 days after planting them in the greenhouse for two seasons to water treatments, using three levels of irrigation stress for the plants, and some morphological, physiological, and biochemical parameters were studied, and finally, we employed gene expression techniques such as quantitative real-time polymerase chain reaction (Q-RT-PCR).</p><p><strong>Results: </strong>Ten sugarcane genotypes were exposed to three stressed water treatments in the current investigation based on crop evapotranspiration. The results demonstrated that morphological measurements revealed highly significant variations in the majority of genotypes under the most severe water deficit stress, which was 62.5% of ET₀. Significant variations are evident in physiological parameters (relative water content and chlorophyll), with the majority of genotypes exhibiting high values for the parameters under investigation (G.2000-3 and G.2004-27). For biochemical measurements, the genotypes with the highest excellent values were G.2003-49 and G.2000-3 for peroxidase and G.84-47 for proline concentration and total phenolic compounds. However, at the molecular level, the genotypes K.81113 and G.2004-27 outperformed the others in sustaining an increase in sucrose synthase and proline oxidase (POX). Regarding sugarcane production and yield components, the genotype that was most tolerant under the maximum stressed irrigation level of 62.5% of ET₀ was G.99-103. This genotype exhibited good values for the examined parameters under all stressed conditions, including 1 (ScSuSy1) gene. Genotypes G.2009-11, G.2003-47, G.2003-49, and M.35-15 contributed the most to principal component analysis, accounting for 62.5% of ET₀.</p><p><strong>Conclusion: </strong>The most promising genotypes for drought tolerance, increased capacity for sucrose accumulation, and potential application in breeding programs are G.2004-27, K.81113, G.2000-3, G.99-103, and G.2009-11, based on all the data obtained at all the parameters evaluated.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"504"},"PeriodicalIF":2.6,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144143045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Nobiletin potentially reduce lipid accumulation by up-regulating the SIRT1-AMPK signaling pathway in HepG2 hepatocarcinoma cells.","authors":"Hajar Shokri-Afra, Elham Yousefi Abdolmaleki, Elnaz Sadat Mousavi Sadr Jadidi, Ziaeddin Oladi, Hemen Moradi-Sardareh, Mohsen Nabi Afjadi, Davod Ilbeigi, Haleh Barmaki","doi":"10.1007/s11033-025-10587-z","DOIUrl":"https://doi.org/10.1007/s11033-025-10587-z","url":null,"abstract":"<p><strong>Background: </strong>The prevalence of nonalcoholic fatty liver disease (NAFLD) is rising at an alarming rate, making it a major global public health problem. The main pathophysiology of NAFLD is elevated de novo lipogenesis (DNL) in hepatocytes which leads to lipid accumulation. Because of their function in controlling DNL, sirtuin 1 (SIRT1) and AMP-activated protein kinase (AMPK) have been considered viable therapy targets for reduce lipid accumulation.</p><p><strong>Methods and results: </strong>We examined the impact of the citrus flavonoid nobiletin (NOB) on the SIRT1-AMPK signaling pathway. This study involved incubating HepG2 cells with varying concentrations of NOB, measuring SIRT1 gene expression using qRT-PCR, assessing SIRT1 enzyme activity using a fluorometric assay, determining SIRT1 protein and AMPK phosphorylation levels by Western blotting, and measuring the lipid profile using semi- and quantitative assays. The results demonstrated that NOB significantly induced SIRT1 mRNA, protein expression, and activity similar to resveratrol (RSV) (as positive controls); additionally, NOB increased the phosphorylation of AMPK. EX-527 (negative control) significantly reversed the stimulatory effect of NOB on SIRT1 and AMPK. On the other hand, NOB decreased total lipid accumulation in cells exposed to oleic acid (OA) and reduced TG content to a normal level. However, the observed results on lipid profile were counteracted in the presence of EX-527.</p><p><strong>Conclusions: </strong>NOB might be a new therapeutic approach for lipid accumulation management due to inducing the SIRT1-AMPK signaling pathway, however, it requires further investigations.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"503"},"PeriodicalIF":2.6,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144142975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antidepressant-like and neuroprotective effect of hydroalcoholic extract of Aloysia citriodora in animals subjected to childhood stress.","authors":"Amanda Gollo Bertollo, Silvio José Batista Soares, Severina Silva Amaral, Jesiel de Medeiros, Ana Olivia Albino Nicolleti, Hélio Jungkenn Ibrahim, Kelli Maria Kreuz, Laysa Anacleto Schuh, Brunna Varela da Silva, Maiqueli Eduarda Dama Mingoti, Walter Antônio Roman Junior, Lilian Caroline Bohnen, Gilnei Bruno da Silva, Daiane Manica, Margarete Dulce Bagatini, Zuleide Maria Ignácio","doi":"10.1007/s11033-025-10576-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10576-2","url":null,"abstract":"<p><strong>Background: </strong>Stress in childhood can harm brain development, leading to biological and systemic dysfunctions that, along with genetic factors, may contribute to major depressive disorder (MDD) in adulthood. MDD treatment is complex, with current drugs taking time to show effects and not working for many patients. As a result, medicinal plants, like Aloysia citriodora (A. citriodora), are being studied as treatments for resistant depression. The main objective of this proposal was to evaluate the treatment with the hydroalcoholic extract of the medicinal species A. citriodora Palau on depressive-like behaviors in rats subjected to chronic stress in childhood and on mechanisms involved in neuroprotection through the evaluation of neuroinflammation and oxidative stress.</p><p><strong>Methods: </strong>The maternal deprivation (MD) protocol was used during the first ten days of life. At the beginning of adulthood, the animals were treated for fourteen days. At the end of the treatments, the animals underwent the forced swimming behavioral test and were then euthanized for analysis of oxidative stress and inflammation markers.</p><p><strong>Results: </strong>The stress caused by the MD protocol in rats culminated in a significant increase in depressive-like behaviors in adulthood, an increase in the expression of inflammatory substances IL-1 β and IL-6 in the animals' hippocampus, and an increase in the activity of oxidative stress parameters, such as myeloperoxidase (MPO) and thiobarbituric acid reactive substances (TBARS), in serum and hippocampus. The treatment with A. citriodora reduced depressive-like behaviors similar to the group treated with escitalopram. Regarding markers of inflammation in nervous tissue, it was observed that animals treated with A. citriodora had lower levels than those treated with escitalopram and the control group, and even more significantly when compared to the MD group treated with saline. The treatments reduced MPO activity in the serum of animals that underwent the MD protocol. TBARS was decreased in the serum and ultimately reversed in the hippocampus. Moreover, molecular docking studies have demonstrated that baicalein and quercetin-7-O-glucoside interact efficiently with IL-1β and IL-6.</p><p><strong>Conclusion: </strong>PM stress increased depressive-like behavior and increased markers of oxidative stress and inflammation. Treatment with the Aloysia citriodora extracts significantly reduced depressive-like behavior and markers of oxidative stress and inflammation, suggesting an antidepressant, antioxidant, and anti-inflammatory effect.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"498"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic modification of mesenchymal stem cells (MSCs): novel strategy to expand their naïve applications in critical illness.","authors":"Hajar Nasiri, Tahereh Manoochehrabadi, Fatemeh Eskandari, Jila Majidi, Mazaher Gholipourmalekabadi","doi":"10.1007/s11033-025-10570-8","DOIUrl":"https://doi.org/10.1007/s11033-025-10570-8","url":null,"abstract":"<p><p>Mesenchymal stem cells (MSCs) have emerged as a promising option for gene and cell therapy due to their unique biological properties. MSC-based cell therapies have garnered significant attention for various clinical applications; however, repeated administrations are often necessary to achieve sustained therapeutic effects. Genetic modification techniques have enhanced MSCs' intrinsic capabilities, improving their therapeutic efficacy in both experimental and clinical settings. Key functional properties anti-inflammatory, anti-fibrotic, survival, and migratory capacities have become central targets for genetic enhancement. Numerous studies have explored the genetic modification of MSCs to address overcoming the transient nature of their therapeutic effects. Notably, the safety of genetically engineered MSCs remains a critical concern in preclinical and clinical investigations.In this review, we summarize current strategies for the genetic modification of MSCs and discuss recent findings on their application in animal disease models.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"501"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative morphometric and phylogenetic insights into Eastern Ghats channids (Teleostei: Channidae) unveil the transboundary dispersal of the Dwarf Snakehead (Channa kelaartii) across India and Sri Lanka.","authors":"Boni Amin Laskar, Harikumar Adimalla, Dhriti Banerjee, Se Hyun Song, Hye-Eun Kang, Hyun-Woo Kim, Shantanu Kundu","doi":"10.1007/s11033-025-10610-3","DOIUrl":"https://doi.org/10.1007/s11033-025-10610-3","url":null,"abstract":"<p><strong>Background: </strong>The biogeography of Eastern Ghats channids, including the Dwarf Snakehead Channa kelaartii, poses a complex challenge, especially with recent evidence confirming its presence in both India and Sri Lanka. While the Sri Lankan population is well- documented through integrative approach, the Indian population remains unexplored, requiring further research to elucidate its genetic structure and phylogenetic relationships.</p><p><strong>Methods: </strong>This study examines the morphology and mtCOI-based genetic diversity of 10 channid species, including seven from the Gachua complex. An integrative approach is also applied to assess population-level variation in C. kelaartii, aiming to clarify its genetic diversity with geographically isolated Sri Lankan populations.</p><p><strong>Results: </strong>Morphological analysis distinctly identified all Channa species and indicated a close resemblance between Indian and Sri Lankan C. kelaartii populations. Genetic analysis revealed considerable divergence between C. kelaartii and other members of the Gachua group, ranging from 6.15 to 21.31%. Bayesian phylogenetic reconstruction robustly resolved all species, including C. kelaartii. Combined mitochondrial data from India and Sri Lanka revealed 13 haplotypes, with mean intra-regional genetic distances of 0.5% (Sri Lanka) and 0.7% (India), and maximum divergences of 1.24% and 2.29%, respectively. A haplotype from the Western Ghats exhibited only 0.17% divergence from Sri Lankan populations, indicating potential historical gene flow between the two regions.</p><p><strong>Conclusions: </strong>Overall, this study provides a comprehensive assessment of Channa diversity in the Eastern Ghats and confirms the presence of C. kelaartii in both peninsular India and Sri Lanka, likely shaped by historical land connections and freshwater dispersal across the Palk Isthmus during the Plio-Pleistocene.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"499"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genistein as a renoprotective agent: mechanistic insights into antioxidant, anti-inflammatory, and fibrosis-regulating pathways.","authors":"Ayush Kattna, Lovedeep Singh","doi":"10.1007/s11033-025-10603-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10603-2","url":null,"abstract":"<p><p>Kidney diseases refer to a group of disorders that affect the structure and function of the kidneys, impairing their ability to filter waste products, excess fluids, and toxins from the blood. These diseases can be acute and chronic, and if left untreated, can lead to kidney failure. Their progression is closely associated with inflammation and oxidative stress. Key signaling cascades, such as TLR-4/MAPK and TLR-4/NF-κB, are instrumental in fostering renal inflammation. Excessive ROS production worsens kidney damage, whereas activation of the Nrf-2/ARE pathway mitigates this by enhancing antioxidant defense. Moreover, the TGF-β/Smad pathway is heavily implicated in driving renal fibrosis, a major factor in disease progression. Additionally, elevated uric acid levels exacerbate inflammatory signaling, thereby worsening renal injury and dysfunction. Current treatments for kidney diseases have several concerns, including the need for routine monitoring, side effects, and long-term regimens. Several natural compounds have shown promise in supporting kidney health by modulating these key molecular targets. Genistein is a naturally occurring isoflavone predominantly found in soybeans and soy-based products, such as tofu, soy milk, and tempeh. It has demonstrated beneficial effects in various renal disorders, including both acute and chronic conditions, by regulating key molecular mediators involved in tissue injury, fibrosis, and cellular defense mechanisms. These mediators include TLR-4, MAPK, NF-κB, TGF-β, Smads, ACE, angiotensin, SIRT1, Nrf-2, ROS, SERBP, JAK/STAT and cytokines, among others. Considering the potential of genistein in modulating these mediators, the current review investigates the mechanistic interactions among these mediators in mediating its renoprotective effects.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"500"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theranostic potential of extracellular vesicles in reproductive tracts: implications for recurrent implantation failure.","authors":"Fatemeh Ziyafati Kafi, Nasim Eslami, Faezeh Shekari, Masood Bazrgar","doi":"10.1007/s11033-025-10619-8","DOIUrl":"https://doi.org/10.1007/s11033-025-10619-8","url":null,"abstract":"<p><p>Embryo implantation is a critical step at the beginning of pregnancy, occurring during a specific and limited period known as the \"implantation window\". Successful implantation involves various signaling pathways and molecular interactions. Recent studies have highlighted the importance of extracellular vesicles (EVs) in mediating these complex interactions. Different cell types release EVs to transfer signals to other cells or tissues. Additionally, emerging evidence suggests that EVs regulate signaling between the developing embryo and endometrium. In this review, we summarize current findings that highlight the role of EVs in the reproductive tract, gamete production, and their potential roles in embryo development and implantation. We then examine studies emphasizing the role of EVs in embryo-maternal interactions and implantation. Finally, we will explore the theranostic potential of EVs in various aspects of assisted reproductive technology (ART), including modulation of embryo-maternal interactions, enhancement of embryo quality, and improvement of endometrial receptivity. A more comprehensive understanding of EVs in the pathology of recurrent implantation failure could support the development of personalized treatments.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"502"},"PeriodicalIF":2.6,"publicationDate":"2025-05-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rewriting the urinary tract paradigm: the urobiome as a gatekeeper of host defense.","authors":"Agnieszka Pastuszka, Szymon Tobor, Igor Łoniewski, Anna Wierzbicka-Woś, Katarzyna Sielatycka, Daniel Styburski, Danuta Cembrowska-Lech, Tomasz Koszutski, Marek Kurowicz, Klaudia Korlacka, Albert Podkówka, Artur Lemiński, Andrzej Brodkiewicz, Lidia Hyla-Klekot, Karolina Skonieczna-Żydecka","doi":"10.1007/s11033-025-10609-w","DOIUrl":"10.1007/s11033-025-10609-w","url":null,"abstract":"<p><p>The urobiome, or urinary tract microbiome, has emerged as a crucial component in maintaining urinary health and defending against infections. Recent advances in next-generation sequencing (NGS) have debunked the long-held belief that the urinary tract is sterile, revealing a unique ecosystem of microorganisms. The urobiome interacts with the urothelium and mucosa-associated lymphoid tissue (MALT) to support local immunity, playing an integral role in defending the urinary tract against pathogens. Through complex communication processes like quorum sensing, the urobiome regulates microbial behavior and controls interactions with host tissues, helping to prevent pathogen colonization and infection. However, dysbiosis in the urobiome can disrupt this balance, making the urinary tract more susceptible to infections, including urinary tract infections (UTIs). Studies have highlighted specific microbial compositions associated with both healthy and disease states, suggesting that shifts in the urobiome may correlate with various urological diseases. Furthermore, microbial diversity within the urinary tract differs by factors such as age and gender, reflecting the dynamic nature of the urobiome. Future research focusing on the interplay between the urobiome, host immune defenses, and pathogenic mechanisms may lead to innovative diagnostic and therapeutic approaches. Understanding how microbial composition changes during disease states could enable targeted treatments, potentially reducing reliance on antibiotics and minimizing resistance issues. The urobiome thus represents a promising frontier in urology, with implications for enhancing urinary health and treating infections more effectively.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"497"},"PeriodicalIF":2.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12102141/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A miR-770-5p/XBP1-driven pathway controls ESR1 expression and tamoxifen response in luminal breast cancer.","authors":"Senem Noyan","doi":"10.1007/s11033-025-10598-w","DOIUrl":"https://doi.org/10.1007/s11033-025-10598-w","url":null,"abstract":"<p><strong>Background: </strong>Cancer cells employ various adaptive mechanisms to withstand stressors, with one notable pathway being the unfolded protein response (UPR), crucial in fostering endocrine resistance within ER-positive breast cancer. Investigating miRNAs within tumors holds promise for identifying key miRNA-gene interactions pivotal for tumor characteristics like proliferation and resistance to treatment. Notably, XBP1 emerges as a pivotal player in UPR within the endoplasmic reticulum, particularly through the inositol-requiring enzyme 1 (IRE1α) - the X-box-binding protein 1 (XBP1) pathway, presenting a compelling target for clinical intervention. In this study, I explore the regulatory role of miR-770-5p in modulating XBP1 expression and its potential as a therapeutic target in luminal breast cancer.</p><p><strong>Methods and results: </strong>The role of XBP1 in cancer cell pathogenesis has been investigated using bioinformatics tools to analyze its expression in breast cancer samples and its association with clinical outcomes. The relationship between miR-770-5p and XBP1 was further explored through bioinformatics analysis and ROC plot assessment. qRT-PCR and bioinformatics data confirmed the negative correlation between miR-770-5p and XBP1, particularly in Luminal A subtype with wild-type p53. My results demonstrated that miR-770-5p targets XBP1, inhibiting its spliced form and reducing its downstream transcriptional activity. Moreover, I found that miR-770-5p can modulate tamoxifen sensitivity by influencing the XBP1/ESR1 axis in breast cancer cells. Overexpression of miR-770-5p led to decreased ESR1 levels, enhancing tamoxifen efficacy and suggesting a potential novel therapeutic approach for luminal subtype breast cancer.</p><p><strong>Conclusions: </strong>miR-770-5p has a growth-inhibitory function in luminal breast cancer, where its suppression of XBP1 contributes to the increased sensitivity to tamoxifen.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"496"},"PeriodicalIF":2.6,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144128352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}