Molecular Biology Reports最新文献

{"title":"First microsatellite markers for the Amazonian species Copaifera multijuga Hayne developed using next-generation sequencing.","authors":"Rayssa Gomes Vasconcelos, Sandra Marcela Hernández-Rangel, Izeni Pires Farias, Tomas Hrbek, Paulo de Tarso Barbosa Sampaio, Raquel da Silva Medeiros, Antonio Saulo Cunha-Machado","doi":"10.1007/s11033-024-10192-6","DOIUrl":"https://doi.org/10.1007/s11033-024-10192-6","url":null,"abstract":"<p><strong>Background: </strong>Native to the Amazon region, Copaifera multijuga Hayne is a large tree (≈ 36 m in height) that is heavily exploited for extraction of its oleoresin. Many studies have addressed the phytochemical properties and applications of this raw material; however, there are few initiatives that have focused on the genetic characterization of native populations of this species. To this end, our objective was to develop microsatellite markers for C. multijuga, which were previously unavailable, and apply them to the characterization of a native population located in the Adolpho Ducke Forest Reserve (Manaus, Amazonas, Brazil).</p><p><strong>Methods and results: </strong>Using next-generation sequencing technology on the Ion Torrent PGM™ platform, 19 pairs of microsatellite primers were designed and developed. For the characterization and validation, we used a group of 47 C. multijuga trees. After preliminary tests, amplification conditions were standardized for 14 loci. The CmH05 locus was excluded from the analyses for being monomorphic, and the remaining loci were used to estimate key genetic parameters for the species, such as observed (Ho) and expected heterozygosity (He), total number of alleles (A), fixation index (f), and polymorphic information content (PIC), among others. The population showed levels of genetic diversity that were higher than 0.63 (Ho: 0.67; He: 0.64) and an average number of 5.4 alleles. According to the PIC estimates, all loci were considered highly (9) or moderately informative (3), except for CmH11, which had a value that was below 0.2.</p><p><strong>Conclusions: </strong>The 13 developed microsatellites were efficient in characterizing the genetic diversity of C. multijuga and may be advantageous in future investigations aimed at defining effective conservation strategies for the species.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"92"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing tick diversity among caprine hosts of Kerala, India: a phylogenetic study.","authors":"Sasi Adithya, Andrews Megha, Y Ajith, K S Athira, Anna-Maria Ettel, S Gireesh Sangeetha, Siju Susan Jacob, P V Tresamol","doi":"10.1007/s11033-024-10191-7","DOIUrl":"https://doi.org/10.1007/s11033-024-10191-7","url":null,"abstract":"<p><strong>Background: </strong>Ticks are prominent vectors of numerous pathogens that adversely affect human and animal health. Monitoring tick population dynamics is key in developing ideal tick-borne disease surveillance systems and critical vector control programmes. This study aimed to conduct the morphological and molecular characterization of ticks infesting domesticated goats in Kerala, India.</p><p><strong>Methods and results: </strong>A total of 30 goats presented to the small ruminant unit of the Teaching Veterinary Clinical Complex (TVCC), Mannuthy were randomly screened for tick infestation, with 22 (73.3%) found to be infested. Morphological identification of different life cycle stages and genera was conducted first, utilizing documented external characteristics such as body size, presence of eyes, and other key morphological traits. A total of 153 tick samples were collected from goats, and their identification revealed that they belonged to the genera Haemaphysalis and Rhipicephalus. This was followed by molecular analysis through sequencing a fragment of the cytochrome c oxidase subunit I (COXI) gene, a standard marker for tick identification. The results from molecular and phylogenetic analyses confirmed the tick species as Haemaphysalis bispinosa, H. intermedia, Rhipicephalus haemaphysaloides, and R. sanguineus. The sequenced specimens were deposited in the NCBI GenBank contributing to the global understanding of tick distribution and diversity in goats. The GenBank accession no. (s) of the isolates are PQ433166 (H. bispinosa), PQ433290 (H. intermedia), PQ433525 (R. haemaphysaloides), and PQ433586 (R. sanguineus).</p><p><strong>Conclusions: </strong>The findings of this study contribute to a better understanding of the tick fauna infesting goats in the region and emphasize the importance of developing research and monitoring plans to address the challenges posed by these ectoparasites. It also highlights a critical area for future study, targeting the vector potential of these arthropods in hemoparasitic diseases and zoonotic disease transmission.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"89"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of novel microsatellite marker sets for 5 common tropical hydrozoan species (Cnidaria: Hydrozoa) from the Southwestern Indian Ocean.","authors":"David Ory, Clara Mouronvalle, Pascale Chabanet, Chloé A-F Bourmaud, Emilie Boissin","doi":"10.1007/s11033-024-10179-3","DOIUrl":"https://doi.org/10.1007/s11033-024-10179-3","url":null,"abstract":"<p><strong>Background: </strong>In the context of global change, coral reefs and their associated biodiversity are under threat. Several conservation strategies using population genetics have been explored to protect them. However, some components of this ecosystem are understudied, such as hydrozoans, an important class of benthic organisms worldwide. A comprehensive study of coral reefs as a whole is needed to develop effective conservation measures. Here we describe the development of 75 new microsatellite markers for 5 hydroid species: Antennella billardi, Lytocarpia phyteuma, Sertularella diaphana, Taxella gracilicaulis and Zygophylax rufa.</p><p><strong>Methods and results: </strong>Markers were tested on 246 specimens from Reunion and Mayotte islands in the southwestern Indian Ocean. Allelic diversity ranged from 1 to 21 for the 5 species, and 9 loci were estimated to have null allele frequencies ranging from 10 to 37%. Observed and expected heterozygosities ranged from 0.03 to 1 and from 0.03 to 0.93, respectively. 12 loci showed data significantly out of Hardy-Weinberg equilibrium. Cross-amplification was performed in 8 species, of which 3 showed high successful amplification rates (53 to 93%).</p><p><strong>Conclusion: </strong>The estimated genetic metrics were similar to those reported for other hydroid and cnidarian marker sets. Cross-amplification showed a contrasting transferability between species, often related to the hydroid phylogeny. These newly developed markers will be relevant to the study of hydroid population genetics and coral reef conservation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"91"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142908867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"MicroRNAs as diagnostic biomarkers in diabetes male infertility: a systematic review.","authors":"Zeinab Latifi, Saba Nikanfar, Rasa Khodavirdilou, Sohrab Minaei Beirami, Lida Khodavirdilou, Amir Fattahi, Farnaz Oghbaei","doi":"10.1007/s11033-024-10197-1","DOIUrl":"https://doi.org/10.1007/s11033-024-10197-1","url":null,"abstract":"<p><p>This study conducts an in-depth review of the correlation between testis tissue changes and circulating microRNAs (miRNA) in diabetes-induced male reproductive complications, drawing upon both animal and clinical studies. The original articles published in English that specifically investigate miRNAs linked to male infertility in humans or animals with either type I or ΙΙ diabetes mellitus were included. The relevant articles were gathered from the PubMed, Google Scholar, Cochrane Library, and ScienceDirect databases. The quality of study was assessed utilizing the Joanna Briggs Institute (JBI) Critical Appraisal Checklist for Prevalence Studies. We collected an overall number of 1989 citations relating to our research subject. Following the elimination of articles based on the criteria, a total of 20 papers were included in the study. Aberrant expression profiles of 25 miRNAs were identified in diabetes associated with male reproductive issues, with 15 miRNAs exhibiting increased expression and 10 miRNAs showing decreased expression. Among the chosen publications, eighteen were identified as low-risk and two were classed as moderate quality. The dysregulated miRNAs were linked to testicular injury, disrupted steroid production, decreased sperm development and quality, and erectile dysfunction. The results demonstrate that the miRNA-mRNA network is linked to the pathological progression of diabetic testicular damage or erectile dysfunction. From a therapeutic perspective, the identification of circulating miRNAs could be beneficial in the timely identification and prevention of diabetes problems, such as diabetes-induced male infertility. Among all signaling pathways influenced by modified miRNAs, the Bax-caspase-3, MAPK, PI3K-Akt, and eNOS-cGMP-PKC were the main deregulated pathways.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"90"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of cataranthine treatment on miRNA34 and miRNA29 levels in HepG2 cells and their association with the expression levels of Bcl-2 and Nrf2.","authors":"Nafiseh Heidari-Kalvani, Fariba Mehdikhani, Yaser Mohammadi, Fereshteh Barjesteh, Elham Bahreini","doi":"10.1007/s11033-024-10176-6","DOIUrl":"https://doi.org/10.1007/s11033-024-10176-6","url":null,"abstract":"<p><strong>Introduction: </strong>Cataranthine is an alkaloid used in the development of anti-cancer drugs. In this study, the effect of cataranthine is assessed by measuring the levels of miR-34 and miRNA-29, which are effective regulators of BCL-2 and NRF-2 gene expression, and their relation to the survival of HCC cells.</p><p><strong>Methods: </strong>This study used cataranthine, and the HepG2 cell line. The MTT test was used to determine the appropriate concentration of cataranthine for treatment (IC50). Oxidative stress status was assessed by evaluating TAC (total antioxidant capacity), TOS (total oxidant status), and MAD (malondialdehyde) levels. Flow cytometry was used to investigate apoptosis. The expression levels of Nrf2, Bcl2, miRNA34, and miRNA29 genes in HepG2 were evaluated by RT-PCR.</p><p><strong>Results: </strong>We observed that cataranthine significantly reduced the levels of oxidative markers (MAD, and TOS) and, conversely, increased the level of antioxidant markers in HepG2 cells. Treatment of HepG2 cells with different doses of cataranthine significantly increased the expression of Nrf2 and Bcl-2 genes, while significantly decreasing the expression of miR29 and miR34 genes.</p><p><strong>Conclusion: </strong>These findings suggest that cataranthine may exert its anticancer effects by reducing oxidative stress and promoting apoptosis, while decrease in miR34 and miR29 as well as increase in Nrf2 and Bcl2 may act as resistance mechanisms in cancer cells. The results highlight the dual potential of cataranthine in regulating cellular responses to oxidative stress and cell death in liver cancer, with dose-dependent modulatory effects.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"88"},"PeriodicalIF":2.6,"publicationDate":"2024-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142903401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic and functional role of the nuclear export receptor 1 (XPO1) in gastrointestinal cancers: a potential novel target?","authors":"Viktorija Sokolova, Rebecca Gruber, Lorenz M Pammer, Florian Kocher, Eckhard Klieser, Arno Amann, Renate Pichler, Michael Günther, Steffen Ormanns, Daniel Neureiter, Andreas Seeber","doi":"10.1007/s11033-024-10169-5","DOIUrl":"10.1007/s11033-024-10169-5","url":null,"abstract":"<p><p>In the last decades the survival of metastatic gastrointestinal (GI) cancer patients could have been significantly extended due to the introduction of targeted- and immunotherapy. However, only the minority of patients will experience long-lasting survival. Hence, novel therapeutics are clearly necessary for GI cancer patients. Molecular high-throughput profiling techniques have revealed potential novel targetable molecular alterations, emphasizing the necessity for tailored therapeutic approaches. Nuclear export proteins, particularly Exportin-1 (XPO1), have emerged as promising targets in cancer therapy due to their crucial role in cellular homeostasis and regulation of key cellular functions. Dysregulation of XPO1-mediated nuclear export leads to the functional loss of tumor suppressors and pro-apoptotic factors, facilitating cancer progression. Selinexor, a XPO1 inhibitor, has shown promising activity in preclinical and clinical studies, particularly in hematological malignancies. However, its efficacy in GI cancers remains underexplored. This review aims to elucidate the functional and pathophysiological role of XPO1 in GI cancers. Despite the potential of XPO1 inhibitors in suppressing cell proliferation and inducing apoptosis, comprehensive molecular landscape data and validation of selective inhibitors in GI cancers are lacking. Targeting XPO1 presents a significant therapeutic potential for the treatment of GI cancer patients. Further research is necessary to fully elucidate the molecular landscape according to XPO1 expression in GI tumors and to validate the efficacy of selective XPO1 inhibitors. These efforts are expected to contribute to the development of more effective and personalized therapeutic strategies for GI cancer patients.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"87"},"PeriodicalIF":2.6,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11680630/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Allium chromosome evolution and DNA sequence localization.","authors":"Rafiq Ahmad, Paulina Tomaszewska, M Kausar Nawaz Shah, Nadeem Khan, Danish Ibrar, Naeem Akhtar, J S Pat Heslop-Harrison","doi":"10.1007/s11033-024-10184-6","DOIUrl":"https://doi.org/10.1007/s11033-024-10184-6","url":null,"abstract":"<p><strong>Background: </strong>Molecular cytogenetics, utilizing DNA probes, serves as a critical tool for mapping genes to the physical structures of chromosomes.</p><p><strong>Methods: </strong>In this study, we examined three Allium species: A. cepa L., A. sativum L., and A. fistulosum L., using in situ hybridization to localize 45S rDNA and 5S rDNA genes.</p><p><strong>Results: </strong>We observed variation in both the chromosomal localization and signal intensity of the 45S and 5S rDNA probes across the species. Notably, in A. sativum, additional 5S rDNA signals were detected on chromosome 8, in a heterozygous condition. Additionally, we aimed to explore the feasibility of localizing genes associated with pigment biosynthesis in A. cepa, specifically the PAL and FLS genes. For this, we employed TSA-FISH on both meiotic and mitotic chromosomes. Preliminary results suggested that the PAL gene was localized to meiotic metaphase chromosomes, while the single-copy FLS gene was detected on mitotic chromosomes.</p><p><strong>Conclusion: </strong>The TSA-FISH technique proved neither routine nor robust for consistent localization of these specific probes in plant chromosomes. The findings based on rDNA analysis also offer insights into potential evolutionary implications among the different Allium species studied.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"84"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human umbilical cord mesenchymal stem cells improve the ovarian function through oxidative stress-mediated PERK/eIF-2α/ATF4/CHOP signaling in premature ovarian insufficiency mice.","authors":"Pei-Ling Wu, Shi-Huan Tang, Han-Yue Wang, Hong-Mei Zhang, Lu Peng, Yao Liu, Yuan Yang, Chun-Bing Zheng, Xian-Ping Zhang","doi":"10.1007/s11033-024-10189-1","DOIUrl":"https://doi.org/10.1007/s11033-024-10189-1","url":null,"abstract":"<p><strong>Background: </strong>Premature ovarian insufficiency (POI) is a refractory disease that severely affects female fertility. The PERK/eIF-2α/ATF4/CHOP pathway is one of the classical pathways involved in the unfolded protein response to endoplasmic reticulum stress by regulating protein synthesis and promoting apoptosis. This study aimed to investigate the functional role and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in the POI animal model through the PERK/eIF-2α/ATF4/CHOP pathway.</p><p><strong>Methods and results: </strong>Forty-five sexually mature female C57 mice were divided into a blank control group, POI model group, and hUCMSCs intervention group. To establish the POI model, mice received intraperitoneal injections of cyclophosphamide (CTX) (70 mg/kg) daily for 14 consecutive days, while the control group received saline only. In the hUCMSC intervention group, mice were given hUCMSCs on days 14 and 28, based on CTX modeling in the POI model group. The hUCMSCs were isolated, labeled with 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine iodide (DiR) fluorescent dye, and tail vein-injected, and the distribution of the DiR signal was monitored in the mice using a fluorescence imaging detection method. The ovarian tissues were hematoxylin and eosin stained to observe the ovarian structure, and the number of primordial follicles were counted. An enzyme-linked immunosorbent assay was used to detect the serum levels of estradiol, anti-mullerian hormone, and follicle-stimulating hormone. Terminal deoxynucleotidyl transferase dUTP nick end labeling was used to detect the apoptosis of granulosa cells (GCs). The reactive oxygen species (ROS) content of ovarian tissue was detected by flow cytometry assay. The RNA expression of PERK, eIF-2α, ATF4, and CHOP was determined by quantitative real-time polymerase chain reaction, and protein levels of the targets were determined by western blot and immunohistochemistry. We identified hUCMSCs using surface antigenic markers (CD90, CD44, CD105, and CD73), and osteoblasts and chondroplast differentiation assays. Our studies demonstrated that hUCMSC intervention significantly restored ovarian function by improving the irregular estrous cycle, increasing the number of follicles, decreasing ROS, and inhibiting GC apoptosis in POI mice. Moreover, hUCMSCs suppressed CTX-induced PERK/eIF-2a/ATF4/CHOP pathway activation.</p><p><strong>Conclusions: </strong>HUCMSCs can migrate to the damaged ovaries of POI mice, and improve the ovarian function of POI mice by inhibiting oxidative stress, down-regulating the expression of the PERK/eIF-2α/ATF4/CHOP pathway, and reducing the apoptosis of GCs.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"85"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First evidence of predation on the native endangered Iberian desman (Galemys pyrenaicus) and Iberian water shrews (Neomys anomalus) by the invasive species American mink using eDNA tools in Extremadura (Spain).","authors":"Adriana Ripa, María Jesús Palacios-Gonzalez, José A Díaz-Caballero, Antonio Espinosa, Francisco Javier Zalba, Juan Luis García-Zapata, José Luis Fernádez-García","doi":"10.1007/s11033-024-10160-0","DOIUrl":"10.1007/s11033-024-10160-0","url":null,"abstract":"<p><strong>Background: </strong>Wildlife conservation and management aims to restore population declines, it is the vulnerable or endangered populations who require the greatest conservation efforts. In this context, non-invasive sampling has been evaluated as an option for reporting prey/predator impact. Galemys pyrenaicus is currently threatened throughout its range, and cohabits with Nemys anomalus, in Extremadura (Spain). Predation by American mink and otter has been suggested, but the first one is considered a serious conservation problem. This study has focused on the use of molecular methods based on RT-PCR and DNA sequencing, as it can inform on how invasive predators are harming the desman or the Iberian water shrew, and how these genetic tools can be used to detect predation.</p><p><strong>Methods and results: </strong>Five samples (faecal and stomach contents) were received and RT-PCR assays were performed using TaqMan probes simultaneously targeting the cytochrome b (cytb) genes of G. pyrenaicus and N. anomalus, and the presence of both micromammals as prey was assessed. The predator was identified by Sanger sequencing using the nuclear IRBP gene. The assays provide a powerful tool for identification of invasive species, as in this case, but further confirmation by comparative sequence alignment by BLAST search was also necessary.</p><p><strong>Conclusions: </strong>This study contributes to highlight simultaneously monitor and discriminate predation on specific micromammals with faecal samples of predators. Also supports the use of highly sensitive DNA analysis from samples obtained from predators as an additional methodology to monitor their effects on prey populations.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"82"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elevated serum neprilysin levels in patients with chronic hepatitis C and metabolic dysfunction-associated steatotic liver disease: hepatic oxidative stress as an underlying mechanism.","authors":"Kensuke Kitsugi, Takeshi Chida, Tomohiko Hanaoka, Masahiro Umemura, Maho Yamashita, Jun Ito, Kazuyoshi Ohta, Hidenao Noritake, Takafumi Suda, Kazuhito Kawata","doi":"10.1007/s11033-024-10152-0","DOIUrl":"https://doi.org/10.1007/s11033-024-10152-0","url":null,"abstract":"<p><strong>Background: </strong>Neprilysin (NEP) is a metalloprotease that has become a therapeutic target for the treatment of heart failure and hypertension. However, the significance of NEP in chronic liver diseases has rarely been investigated. In this study, we investigated the serum NEP levels in patients with chronic liver disease and their relationship with clinical parameters.</p><p><strong>Methods and results: </strong>Thirty-seven patients with chronic hepatitis C (CHC) who achieved sustained virologic response (SVR) after antiviral treatment and 73 patients with metabolic dysfunction-associated steatotic liver disease (MASLD) were enrolled. Serum neprilysin levels were measured using an enzyme-linked immunosorbent assay. The median NEP levels were 2.2 ng/mL in CHC and 4.1 ng/mL in MASLD, with the latter being significantly higher. Notably, in patients with MASLD, a significant correlation was observed between NEP and gamma-glutamyltransferase (GGT) levels at baseline. In contrast, there was no significant correlation between NEP levels and progression of liver fibrosis in either group. In the MASLD group, obesity and lifestyle diseases were significantly more prevalent, and the patients exhibited significantly higher NEP levels. In patients with CHC, NEP levels significantly decreased after SVR. NEP mRNA expression in liver tissues was significantly downregulated following SVR. Furthermore, a significant correlation was observed between the degree of NEP and GGT improvement.</p><p><strong>Conclusions: </strong>Elevated NEP levels were observed in both CHC and MASLD groups. Considering the association between NEP levels and obesity, lifestyle diseases, and GGT levels, this suggests that oxidative stress may be involved in the elevation of NEP levels in patients with CHC and MASLD.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"81"},"PeriodicalIF":2.6,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142896171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}