Molecular Biology Reports最新文献_第10页

MicroRNA-451: A multifaceted regulator in cerebral and cardiac ischemia-reperfusion injury - from molecular mechanisms to therapeutic potential. MicroRNA-451：脑和心脏缺血再灌注损伤的多层面调控因子——从分子机制到治疗潜力

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-08 DOI: 10.1007/s11033-025-10756-0

Fei-Xiang Wang, Zu-An Shi, Zi-Hang Yu, Bin Lu, Guo Mu

{"title":"MicroRNA-451: A multifaceted regulator in cerebral and cardiac ischemia-reperfusion injury - from molecular mechanisms to therapeutic potential.","authors":"Fei-Xiang Wang, Zu-An Shi, Zi-Hang Yu, Bin Lu, Guo Mu","doi":"10.1007/s11033-025-10756-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10756-0","url":null,"abstract":"Ischemia-reperfusion injury (IRI) represents a major clinical challenge across various organ systems, with especially severe and often irreversible consequences in cardiac and cerebral tissues. Despite advances in understanding its pathophysiology, effective therapeutic strategies remain limited. MicroRNA-451 (miR-451), a highly conserved non-coding RNA located on human chromosome 17q11.2, has emerged as a critical regulator of key pathophysiological processes underlying IRI, including inflammation, immune cell function, oxidative stress, and programmed cell death. This review comprehensively examines the complex and sometimes paradoxical roles of miR-451 in cardiac and cerebral IRI. We explore miR-451's potential as both a diagnostic biomarker and therapeutic target in IRI management, highlighting innovative approaches such as exosome-mediated miR-451 delivery. Finally, we address critical challenges in translating miR-451-based therapies to clinical practice and outline promising directions for future research. This comprehensive analysis provides a theoretical framework for developing novel miR-451-focused strategies for IRI detection and treatment, with significant implications for improving outcomes in ischemic disorders.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"681"},"PeriodicalIF":2.6,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584363","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rett syndrome: advances in Understanding MeCP2 function, potential gene therapies, and public health implications. Rett综合征：了解MeCP2功能、潜在基因治疗和公共卫生影响的进展

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-08 DOI: 10.1007/s11033-025-10802-x

Nadia E Ali, Nabeela Tariq, Gul Naz, Adil Abalkhail, Tasleem Kausar, Ismail Mazhar, Sana Zia, Amjad I Aqib, Najeeb Ullah Khan

{"title":"Rett syndrome: advances in Understanding MeCP2 function, potential gene therapies, and public health implications.","authors":"Nadia E Ali, Nabeela Tariq, Gul Naz, Adil Abalkhail, Tasleem Kausar, Ismail Mazhar, Sana Zia, Amjad I Aqib, Najeeb Ullah Khan","doi":"10.1007/s11033-025-10802-x","DOIUrl":"10.1007/s11033-025-10802-x","url":null,"abstract":"Rett syndrome (RTT) is a devastating X-linked neurodevelopmental disorder, primarily affecting females, caused by mutations in the MECP2 gene. After a brief period of normal development, affected children experience rapid regression, losing motor and communication skills. Core features include microcephaly, seizures, stereotypic hand movements, and breathing abnormalities. While rooted in neurological dysfunction, growing evidence reveals RTT's widespread impact extends beyond the brain, implicating MECP2 in multisystem disruption. This review provides a comprehensive overview of RTT's genetic and neuropathological basis and highlights the significant advances in gene therapy to restore MECP2 function. Notably, adeno-associated virus (AAV)-based approaches have shown promise in preclinical models by improving survival and motor function in RTT mouse models. Recent advancements in AAV vector design have optimized targeted delivery to neurons and enhanced the regulation of MECP2 expression to prevent overexpression-related toxicity. Additionally, nanoparticle-based delivery systems are being explored as non-viral alternatives, offering the potential for improved targeting and safety. These advancements in gene therapy hold promise for RTT, bringing the possibility of effective targeted treatments closer to clinical application. As research continues to unravel RTT's complex pathophysiology, emerging therapies may offer new hope for improving patient outcomes and quality of life.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"687"},"PeriodicalIF":2.6,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The upregulation of miR-21-5p in atherosclerotic plaque. miR-21-5p在动脉粥样硬化斑块中的上调。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-08 DOI: 10.1007/s11033-025-10790-y

Selin Unal, Berk Arapi, Suat Nail Omeroglu, Vahid Rouhi, Mehmet Guven

{"title":"The upregulation of miR-21-5p in atherosclerotic plaque.","authors":"Selin Unal, Berk Arapi, Suat Nail Omeroglu, Vahid Rouhi, Mehmet Guven","doi":"10.1007/s11033-025-10790-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10790-y","url":null,"abstract":"Background: Atherosclerosis refers to a complex arterial condition characterized by inflammation and vascular remodeling leading to plaque formation. It is driven by dysregulated inflammatory pathways, endothelial dysfunction, and abnormal cholesterol metabolism associated with lifestyle risk factors. Because atherosclerosis is a major heart disease risk factor, identifying biomarkers is critical. MicroRNAs regulate gene expression and are implicated in disease pathogenesis. This study investigates the roles of miR-193b-3p, miR-21-5p, and miR-484 in atherosclerotic plaque pathogenesis.Methods & results: A gene expression analysis was performed utilizing the reverse RT-qPCR (transcription-quantitative polymerase chain reaction) technique on samples collected from atherosclerotic plaques in the carotid artery (CAP tissue) and non-atherosclerotic internal mammary arteries (IMA tissue) of 50 patients diagnosed with both coronary artery disease and carotid artery disease. A marked difference was found in the levels of miR-21-5p (p = 0.0001), with CAP tissue showing a 21.96-fold increase in miR-21-5p expression compared to IMA tissue. In contrast, the expression levels of the miR-193b-3p and miR-484 genes did not exhibit any significant differences between the CAP and IMA samples. In CAP tissue, there were strong positive correlations observed between miR-193b-3p and miR-484 (r = 0.99, p < 0.0001), miR-484 and miR-21-5p (r = 0.83, p < 0.0001), and miR-193b-3p and miR-21-5p (r = 0.77, p < 0.0001).Conclusions: Altering miRNA expression in the artery offers a promising approach to impact plaque formation through multiple mechanisms, all by targeting a single molecule. Nevertheless, additional studies are required to validate the pharmacological and diagnostic capabilities of these miRNAs.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"686"},"PeriodicalIF":2.6,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584366","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete Chloroplast genome of Mentha aquatica reveals hypervariable regions and resolves phylogenetic position within the genus Mentha. 水生薄荷叶绿体全基因组揭示了高变区，并解决了薄荷属的系统发育位置。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-07 DOI: 10.1007/s11033-025-10789-5

Aboozar Soorni, Mohammad Mehdi Golchini

引用次数: 0

Exploring protein adenylyltransferase as a therapeutic target for combating ESKAPE pathogens in hospital-acquired infections. 探索蛋白腺苷基转移酶作为对抗ESKAPE病原体在医院获得性感染中的治疗靶点。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-07 DOI: 10.1007/s11033-025-10735-5

Reabetswe Maake, Sarah Otun, Ikechukwu Achilonu

{"title":"Exploring protein adenylyltransferase as a therapeutic target for combating ESKAPE pathogens in hospital-acquired infections.","authors":"Reabetswe Maake, Sarah Otun, Ikechukwu Achilonu","doi":"10.1007/s11033-025-10735-5","DOIUrl":"10.1007/s11033-025-10735-5","url":null,"abstract":"Introduction: In the face of increasing antimicrobial resistance, ESKAPE pathogens-Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species-pose a significant threat to public health, particularly in nosocomial settings.Areas covered: This review explores the potential of targeting protein adenylyltransferase (PrAT) as a therapeutic strategy against these multidrug-resistant bacteria. We discuss the mechanisms of PrAT activity, its involvement in reduction-oxidation (redox) homeostasis, and the rationale for its potential as a drug target against ESKAPE pathogens.Expert opinion: PrAT plays an essential role in sustaining the bacterium's redox homeostasis, a vital aspect of bacterial survival, by interacting with glutaredoxin (Grx). Future research should focus on elucidating the specific role of PrAT in ESKAPE pathogens, with an emphasis on studying the enzyme's function and designing targeted inhibitors. This review underscores the importance of continued investigation into PrAT in ESKAPE pathogens as a critical step in addressing the challenges of antimicrobial resistance in clinical practice.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"680"},"PeriodicalIF":2.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12234641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144584360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Interleukin 37 and its polymorphism (rs3811047) in children with type I diabetes. 白细胞介素37及其多态性（rs3811047）在儿童1型糖尿病中的作用

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-07 DOI: 10.1007/s11033-025-10779-7

Amany M Shabaan, Doaa A Gaber, E L Shaimaa Gomaa Ali, Rehab G Abd El-Hamid, Omayma O Abdelaleem

{"title":"Role of Interleukin 37 and its polymorphism (rs3811047) in children with type I diabetes.","authors":"Amany M Shabaan, Doaa A Gaber, E L Shaimaa Gomaa Ali, Rehab G Abd El-Hamid, Omayma O Abdelaleem","doi":"10.1007/s11033-025-10779-7","DOIUrl":"https://doi.org/10.1007/s11033-025-10779-7","url":null,"abstract":"Background: Type 1 diabetes (T1D) is a multifaceted autoimmune condition. Interleukin-37 (IL-37), a cytokine from the IL-1 family, is recognized for its anti-inflammatory effects and its involvement in the progression of various autoimmune diseases (ADs). Despite this, the exact relationships are not fully clear. The objective of this study was to assess serum IL-37 levels and the polymorphism (rs3811047) to investigate its possible role in T1D.Methods: The study involved 46 individuals with Type 1 Diabetes (T1D) and 45 healthy controls. Serum IL-37 levels were quantified using enzyme-linked immunosorbent assay (ELISA). Genomic DNA was extracted with a specialized DNA extraction kit. The specific single nucleotide polymorphism (SNP) in the IL-37 gene (rs3811047) was then genotyped using real-time polymerase chain reaction (RT-PCR).Results: The serum concentrations of IL-37 did not show a significant difference between individuals with T1D and the control group, with a P-value of 0.7. However, our results revealed a significant correlation between T1D and the IL-37 polymorphism (rs3811047). Both the AG and GG genotypes were strongly associated with an elevated risk of developing T1D in comparison to the control group. Furthermore, the frequency of the G allele in rs3811047 was notably higher in the T1D group (56.5%) compared to the control group (32.2%).Conclusions: These findings suggest that the rs3811047 variant in the IL-37 gene is a significant genetic marker linked to an increased susceptibility to T1D. The AG and GG genotypes, along with the G allele, are associated with a higher risk.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"678"},"PeriodicalIF":2.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring genomic signatures of selection for adaptation and resilience in nomadic Belahi cattle in the tropical climate. 探索热带气候下游牧贝拉希牛适应和恢复能力选择的基因组特征。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-07 DOI: 10.1007/s11033-025-10793-9

Shambhuraditya Purushottam Chavan, Kashif Dawood Khan, Ashish Yadav, Rajesh Kumar Gahlyan, Vikas Vohra, Rani Alex, G R Gowane

{"title":"Exploring genomic signatures of selection for adaptation and resilience in nomadic Belahi cattle in the tropical climate.","authors":"Shambhuraditya Purushottam Chavan, Kashif Dawood Khan, Ashish Yadav, Rajesh Kumar Gahlyan, Vikas Vohra, Rani Alex, G R Gowane","doi":"10.1007/s11033-025-10793-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10793-9","url":null,"abstract":"Background: Indigenous cattle in India are known for their resilience to diseases, parasites, and heat stress. Belahi is a newly registered indigenous breed reared by pastoralist communities in the North Himalayan foothills (Shivalik range). It has been naturally selected for adaptability to nomadic grazing, disease resistance, and milk production. This study aimed to identify genomic selection signatures in Belahi cattle.Methods and results: Genome-wide SNP data were analyzed using three intra-population statistics-Tajima's D, Integrated Haplotype Score (iHS), and Nucleotide Diversity-which were combined into De-correlated Composite of Multiple Signals (DCMS). Additionally, Runs of Homozygosity (ROH) were used to detect regions under putative selection. DCMS identified 290 significant SNPs, while ROH revealed 8 overlapping regions. A total of 822 and 339 protein-coding genes were identified from DCMS and ROH, respectively, with 15 genes overlapping. Key genes included IL2 and IL21 (immune response) on BTA 17, and DNAJB13 (stress-related protein-folding) on BTA 15. QTL analysis revealed associations with tick resistance, susceptibility to respiratory and mycobacterial diseases, and pigmentation traits. Significant pathways included interleukin-2 receptor binding, leukocyte-mediated immunity, and peptidyl-tyrosine phosphorylation.Conclusions: The results suggest that Belahi cattle have undergone natural selection for immunity and environmental stress tolerance due to their nomadic lifestyle. These genomic insights support the breed's potential use in improving disease resistance and climate adaptability in cattle breeding programs.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"676"},"PeriodicalIF":2.6,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanism of hydroxychloroquine in the treatment of obstetric antiphospholipid syndrome by inhibiting NETs-induced trophoblast pyroptosis. 羟氯喹通过抑制nets诱导的滋养细胞焦亡治疗产科抗磷脂综合征的机制。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-07 DOI: 10.1007/s11033-025-10765-z

Jiayue Liu, Mingyang Xu, Di Shen, Xietong Wang

引用次数: 0

Characterization of two glycosyltransferases that modulate withanolide biosynthesis and defense in Withania somnifera. 两种糖基转移酶调控苦参草内酯生物合成和防御的特性研究。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-05 DOI: 10.1007/s11033-025-10743-5

P Anjali, Ananth Krishna Narayanan, Durgesh Parihar, Anusha Patil, Dinesh A Nagegowda

{"title":"Characterization of two glycosyltransferases that modulate withanolide biosynthesis and defense in Withania somnifera.","authors":"P Anjali, Ananth Krishna Narayanan, Durgesh Parihar, Anusha Patil, Dinesh A Nagegowda","doi":"10.1007/s11033-025-10743-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10743-5","url":null,"abstract":"Background: The medicinal properties of Ashwagandha (Withania somnifera) are attributed to the presence of triterpenoid withanolides and their glycosylated forms, withanosides. Withanosides are proposed to be formed from withanolides by the action of glycosyltransferases (GTs).Methods and results: Two genes encoding GTs (WsGT4 and WsGT6) from W. somnifera were identified and characterized using Escherichia coli expressed recombinant proteins and in-planta studies. Biochemical assays with recombinant proteins showed that WsGT4 catalyzed product formation using withanolide A, withanolide B, withanone, and 12-deoxywithastramonolide as substrates and UDP-glucose serving as the glucose donor. While, WsGT6 catalyzed the product formation only with withaferin A as a substrate employing either UDP-glucose or UDP-galactose as sugar donors. Quantitative real-time analysis showed that transcripts of WsGT4 and WsGT6 were induced in response to methyl jasmonate treatment and prominent in leaves as compared to other tissues. Modulating the expression of WsGT4 and WsGT6 by virus-induced gene silencing (VIGS) and transient overexpression significantly affected the levels of withanolides and withanosides in the leaves. Furthermore, silencing either WsGT4 or WsGT6 in W. somnifera reduced the tolerance to Pseudomonas syringae DC3000 growth, while their overexpression enhanced the tolerance to the bacterium.Conclusions: Our results indicate the role of WsGT4 and WsGT6 in withanoside biosynthesis and in defense against a bacterial pathogen in W. somnifera. These GTs could be utilized for targeted modulation of withanolides in cell cultures or at the whole-plant level and for enhancing tolerance against bacterial pathogens and improving the yield of withanosides.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"675"},"PeriodicalIF":2.6,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review on current theories and potential therapies for prion diseases. 朊病毒疾病的最新理论和潜在治疗方法综述。

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-07-04 DOI: 10.1007/s11033-025-10754-2

Saranya Udayakumar, Agnishwar Girigoswami, Koyeli Girigoswami

引用次数: 0