Molecular Biology Reports最新文献

{"title":"Impact of soft tissue homogenization methods on RNA quality.","authors":"Julia Ostapowicz, Bartosz Maćkowiak, Kamila Ostrowska, Barbara Kaczmarek, Natalia Pietras, Dawid Frąckowiak, Magdalena Fundowicz, Katarzyna Kulcenty, Wojciech Golusiński, Wiktoria Suchorska","doi":"10.1007/s11033-025-10508-0","DOIUrl":"https://doi.org/10.1007/s11033-025-10508-0","url":null,"abstract":"<p><strong>Background: </strong>High-quality RNA isolation from tissues is crucial for transcriptomic analysis. The tissue disruption influences the RNA quality. The aim of this study was to compare different methods of tissue homogenization.</p><p><strong>Methods: </strong>Three homogenization methods were used (mortar and pestle, ball mill, and tissue homogenizer) to disrupt head and neck cancerous tissues, healthy tissues from free margin of head and neck cancer patients, and breast skin from breast cancer patients. The comparison of isolated RNA quantity and quality by measuring the concentration and absorbance, RIN, and Ct values of reference genes were performed.</p><p><strong>Results: </strong>RNA isolated after tissue homogenizer usage has the highest 260/230 ratio (p = 0.02) and concentration (p = 0.02) also RIN values tend to be highest across all studied tissues. There are no significant differences between Ct values across all tissues processed with different homogenization methods; however, the Ct values of GAPDH and S18 are negatively correlated with RIN number (p = 0.002, p = 0.003).</p><p><strong>Conclusion: </strong>The tissue homogenizer is the most suitable for obtaining high-quality RNA across all examined tissues, which is essential in various RNA-based analyses. GAPDH and S18 Ct can indicate the RNA quality measured by RIN values.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"425"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12031802/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144031952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High Glucose-induced transcriptomic changes in human trabecular meshwork cells.","authors":"Shivendra Singh, Srimathi Raghavan, Niketa A Patel, Avinash Soundararajan, Padmanabhan P Pattabiraman","doi":"10.1007/s11033-025-10525-z","DOIUrl":"https://doi.org/10.1007/s11033-025-10525-z","url":null,"abstract":"<p><p>Glaucoma is a leading cause of irreversible blindness, often associated with elevated intraocular pressure (IOP) due to trabecular meshwork (TM) dysfunction. Diabetes mellitus (DM) is recognized as a significant risk factor for glaucoma; however, the molecular mechanisms through which hyperglycemia affects TM function remain unclear. This study investigated the impact of high glucose on gene expression in human TM (HTM) cells to uncover pathways that contribute to TM dysfunction and glaucoma pathogenesis under diabetic conditions. Primary HTM cells were cultured under normoglycemic (5.5 mM) and hyperglycemic (30 mM) conditions for seven days, followed by mRNA sequencing (mRNA-seq) to identify differentially expressed genes, with quantitative PCR (qPCR) used for confirmatory analysis. STRING network analysis was performed to predict potential interactions among upregulated and downregulated genes. mRNA-seq analysis revealed 25 significantly differentially expressed genes in high glucose conditions, including upregulated genes associated with oxidative stress, apoptosis, autophagy, immune response, and fibrosis. Notably, TXNIP gene was significantly upregulated, indicating increased oxidative stress and apoptosis in TM cells, while downregulation of autophagy-related genes, such as HSPA6 and LAMP3, suggests compromised protein quality control. Immune response genes, including CCL7 and CHI3L1, were upregulated, suggesting an inflammatory response to oxidative stress. Increased expression of fibrosis-related genes, such as SNAI1, FGF7, and KRT19, and an increase in ECM proteins like Collagen 1 and FN accumulation and fibril formation suggest increased fibrosis of TM in diabetic conditions, potentially elevating IOP. Metabolic changes in diabetic patients could therefore lead to TM dysfunction, impair aqueous humor outflow, and elevate IOP, thereby increasing glaucoma risk. Targeting oxidative stress and fibrosis pathways offers therapeutic strategies to mitigate glaucoma progression in diabetic populations.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"427"},"PeriodicalIF":2.6,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12031768/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic syndrome and effect of gene polymorphisms: ADIPOQ, AGT, AGTR1, AGTR2, ApoC-III, NR3C1 and GNB3 gene polymorphisms.","authors":"Sacide Pehlivan, Naci Senkal, Yasemin Oyaci, Murat Kose, Mustafa Pehlivan, Sevde Hasanoglu Sayin, Alpay Medetalibeyoglu, Istemi Serin, Tufan Tukek","doi":"10.1007/s11033-025-10518-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10518-y","url":null,"abstract":"<p><strong>Background: </strong>Metabolic syndrome (MetS) is defined as a metabolic dysfunction characterized by the co-occurrence of multiple risk factors. Our study aimed to elucidate the effects of ADIPOQ rs266729, rs17300539, and rs2241766, AGT rs699 and rs4762, AGTR1 rs5186, AGTR2 rs11091046, ApoC-III rs2854116 and rs2854117, NR3C1 rs10052957 and rs41423247, and GNB3 rs5443 gene polymorphisms on MetS.</p><p><strong>Methods and results: </strong>A total of 200 patients who met the diagnostic criteria for MetS at the Internal Medicine Outpatient Clinic of Istanbul University, Faculty of Medicine, between 01.08.2022 and 20.05.2023, and 100 healthy controls were included in our study. ADIPOQ rs17300539 GA, AGT rs699 TT, AGTR1 rs5186 AC, and AGTR2 rs11091046 CC genotypes were more frequently observed in patients diagnosed with MetS compared to healthy controls (p < 0.05 for all). MetS patients with AGT rs699 TT genotype had a higher incidence of hypertension compared to individuals with CC/CT genotype of the same polymorphism (p = 0.047). Individuals with ADIPOQ rs17300539 AA/AG and NR3C1 rs41423247 CC genotypes were found to have higher mean waist circumferences compared to others (p < 0.05 for all). Individuals with ADIPOQ rs17300539 AA/AG, NR3C1 rs10052957 GG, and AGT rs4762 GA/AA genotypes exhibited significantly higher incidence of nephropathy. Individuals with ADIPOQ rs17300539 AA/AG and NR3C1 rs10052957 GG genotypes had a significantly higher incidence of retinopathy compared to others.</p><p><strong>Conclusions: </strong>The study demonstrated that the ADIPOQ rs17300539, AGT rs699, AGTR1 rs5186, and AGTR2 rs11091046 polymorphisms are associated with an increased susceptibility to MetS. Significant results concerning clinical features and complications were also observed. Further research is necessary to elucidate the relationship between susceptibility to MetS, clinical and laboratory characteristics, and epigenetic modifications.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"422"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143982359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosome-based cancer vaccine: a cell-free approach.","authors":"Swarup Sonar, Asmit Das, Ketki Kalele, Vetriselvan Subramaniyan","doi":"10.1007/s11033-025-10519-x","DOIUrl":"https://doi.org/10.1007/s11033-025-10519-x","url":null,"abstract":"<p><p>Despite advancements in medical research, cancer remains a significant and persistent cause of death globally. Cancer vaccine, a novel approach, holds immense promise in development of potentially effective cancer treatment. While the concept of developing cancer vaccines has been explored for decades, significant challenges have hindered their clinical translation. Recent researchers have introduced exosomes as the key element for novel cell-free approach of cancer vaccines. Exosomes are a type of extracellular vesicle (EVs) secreted by various cells. These tiny structures can transport and deliver important biomolecules, such as DNA, RNA, proteins, lipids, and immune-stimulatory molecules, to stimulate the body's anti-tumor immune response. Their biocompatibility, targeting ability, immunogenicity, and a notable capacity to cross biological barriers nominate them as promising candidates for cancer vaccine development by addressing current challenges in cancer therapy. This review explores the current state of knowledge on the efficacy of exosomes from various sources for personalized cancer vaccine development, preclinical and clinical evaluations, along with the strategies to optimize immunogenicity and antigen presentation. We also discuss the challenges and potential solutions for overcoming tumor microenvironment-related hurdles, highlighting the promise of exosome-based approaches for cancer immunotherapy by developing a novel cell-free cancer vaccine in future.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"421"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144024393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative study of various populations of Aporrectodea rosea (Savigny, 1826) based on morphological and molecular analyses (Oligochaeta: Lumbricidae).","authors":"Robabeh Latif, Atabak Roohi Aminjan, Masoumeh Malek, Sergei V Shekhovtsov, Tatiana V Poluboyarova, Maria J I Briones","doi":"10.1007/s11033-025-10511-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10511-5","url":null,"abstract":"<p><strong>Background: </strong>Aporrectodea rosea (Savigny, 1826) is a common earthworm species found in the temperate regions of the Holarctic region. This species exhibits significant intraspecific variation, and several cryptic genetic lineages having been described. The high genetic diversity is likely due to its ecological plasticity and adaptability to different environmental conditions. Integrative approaches that combine morphological, ecological, and molecular data have proven to be useful in resolving taxonomic uncertainties in earthworm species. In this study, we investigated the genetic and morphological diversity of several populations of A. rosea from Iran. By combining molecular analyses with morphological and ecological data, we evaluated whether the observed diversity can be linked to phylogeographic origins (Eurosiberian vs. Mediterranean regions). This integrative approach provides new insights into the taxonomy and evolutionary history of A. rosea, contributing to a better understanding of earthworm biodiversity in the Holarctic region.</p><p><strong>Methods and results: </strong>We evaluated the molecular variation within A. rosea by analyzing both nuclear and mitochondrial markers. The phylogenetic results were combined with morphological data, ecological information, and genetic distances using the COI K2P method to assess species delimitations. Cluster analysis revealed the existence of two distinct morphotypes: a reddish morphotype associated with organic-rich, woodland habitats, and a pale morphotype found in drier grassland environments. Our molecular analyses involving sequencing the mitochondrial COI gene and the nuclear histone H3 gene confirmed the existence of two lineages within A. rosea: a Eurosiberian lineage and a Mediterranean lineage.</p><p><strong>Conclusions: </strong>Our analyses confirmed that nearly all specimens collected from Iran fall within the two established lineages: Mediterranean and Eurosiberian. The observed genetic differences between the two morphotype samples can be related to their phylogeographic origins (Eurosiberian/Mediterranean).</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"423"},"PeriodicalIF":2.6,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144027287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Upregulation of antioxidant genes in antimony-resistant Leishmania tropica clinical isolates.","authors":"Maryam Ahmadpour, Hakimeh Torkian Valashani, Saied Reza Naddaf, Mehdi Mohebali, Homa Hajjaran, Behnaz Ghazineghad, Romana Khosravi, Parisa Mousavi, Elham Kazemirad","doi":"10.1007/s11033-025-10464-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10464-9","url":null,"abstract":"<p><strong>Background: </strong>Increasing resistance to antimonial drugs has become a significant challenge in effective treatment in endemic regions of leishmaniasis. Antioxidant defense plays a crucial role in antimony resistance by combating antimonial-induced oxidative stress. Accordingly, we investigated the transcript levels of some antioxidant genes in antimony-sensitive and resistant clinical Leishmania tropica isolates.</p><p><strong>Methods: </strong>In the current study, 22 Leishmania tropica isolates from ACL patients who presented responsive or unresponsive to antimony were examined. The susceptibility of parasites against SbV and hydrogen peroxide (H₂O₂) was analyzed. We evaluated the transcript levels of five genes, including cytosolic and mitochondrial tryparedoxin (cTXN, mTXN), cytosolic and mitochondrial tryparedoxin peroxidase (cTXNPx, mTXNPx), and ascorbate peroxidase (APX) in antimony-sensitive and resistant L. tropica clinical isolates.</p><p><strong>Results: </strong>The in-vitro susceptibility to SbV in intracellular amastigotes revealed 3.82 times higher IC₅₀ in resistant isolates compared to sensitive ones. The IC₅₀ toward H₂O₂ in resistant isolates was 1.6 times higher than in sensitive ones, positively correlated with SbV IC₅₀ values. The average transcript expression level of cTXNPx, mTXNPx, cTXN, and APX genes significantly increased in resistant isolates by 2.51, 1.69, 2.41, and 2.12-fold compared to sensitive ones. The highest correlation coefficient between the gene expression and SbV IC₅₀ values belonged to the cTXNPx, cTXN, APX, mTXN, and mTXNPx genes, respectively. The average transcript expression level of cytosolic TXN and TXNPx in resistant L. tropica isolates was higher than its mitochondrial counterpart.</p><p><strong>Conclusions: </strong>The data presented here revealed a phenotypic heterogeneity in antioxidant gene expression among L. tropica clinical isolates. Overall, the upregulation of genes involved in antioxidant defense could probably contribute to natural antimony resistance in L. tropica clinical isolates.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"418"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144017739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"4-fluorophenylacetamide acetyl coumarin induces pro-inflammatory M1 macrophage polarization and suppresses the immunosuppressive M2 phenotype through PI3k/AKT/NF-κB modulation.","authors":"Anjali Singh, Lakshmi Pillai, Dhanush Danes, Shweta Umar, Suresh Balakrishnan","doi":"10.1007/s11033-025-10517-z","DOIUrl":"https://doi.org/10.1007/s11033-025-10517-z","url":null,"abstract":"<p><strong>Background: </strong>The tumor microenvironment plays a critical role in cancer progression, with tumor-associated macrophages regulating immune responses. These macrophages can adopt a pro-inflammatory M1 phenotype that suppresses tumor growth or an anti-inflammatory M2 phenotype that promotes progression. Reprogramming macrophages toward the M1 phenotype is a therapeutic strategy. Previous studies showed that 4-Fluorophenylacetamide-acetyl coumarin (4-FPAC), a synthetic coumarin derivative, exhibits cytostatic activity in A549 lung carcinoma cells by modulating reactive oxygen species (ROS), nitric oxide synthase, and signaling pathways, including PI3K/AKT/NF-κB. This study evaluates the impact of 4-FPAC on macrophage polarization.</p><p><strong>Hypothesis: </strong>We hypothesized that 4-FPAC promotes M1 macrophage polarization while suppressing M2 markers through modulation of signaling pathways, thus serving as an immunomodulatory agent.</p><p><strong>Results: </strong>Treatment with 4-FPAC induced M1 polarization in THP1-derived macrophages, evident from morphological elongation, elevated ROS and NO production, and increased IL-12 levels. IL-10 levels and M2 markers (CD163, STAT3, AKT1) were downregulated, while M1 markers (CD80, STAT1, AKT2) were upregulated. Gene expression and western blot analyses revealed activation of P38 and NF-κB pathways and reduced phosphorylated AKT1 levels. In silico docking showed strong interactions of 4-FPAC with regulatory proteins like P38, NF-κB, and AKT1, suggesting pathway modulation.</p><p><strong>Conclusion: </strong>4-FPAC facilitates M1 macrophage polarization and inhibits M2 signaling, demonstrating its potential as an immunomodulatory agent. Coupled with its cytostatic effects on A549 cells, these findings position 4-FPAC as a promising candidate for cancer therapy. Further in vivo studies are warranted to validate its therapeutic potential and explore applications in immunotherapy and inflammation-associated diseases.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"415"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143990386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the relationship between the IL-17 rs2275913, IL-17 rs763780, and the IL-6 rs1800795 genotypes in HIV-positive patients with COVID-19.","authors":"Maryam Nejabat, Mohammad Motamedifar, Ava Hashempour, Mohammadreza Heydari, Zohre Foroozanfar, Mohammad Ali Davarpanah, Gholamreza Daryabor","doi":"10.1007/s11033-025-10502-6","DOIUrl":"https://doi.org/10.1007/s11033-025-10502-6","url":null,"abstract":"<p><strong>Introduction: </strong>The people living with HIV with abnormal immune responses have been identified as a population that is particularly susceptibility to contracting COVID-19. We explored the correlation between gene polymorphisms of IL-17A, IL-17F, and IL-6, and the susceptibility to COVID-19 in individuals with HIV infection.</p><p><strong>Methods: </strong>In this cross-sectional study, 337 HIV-positive patients were included. Serological and molecular tests were done using ELISA and PCR-RFLP methods. Allelic frequency, haplotype analyses, linkage disequilibrium were calculated. A linear regression model was used to analyze the interleukin SNP genotypes in HIV patients with and without COVID-19.</p><p><strong>Results: </strong>A total of 337 PLWH were recruited for this study, with 170 having COVID-19 and 167 not having it. The mean age and laboratory indicators showed no significant differences between the two groups (P > 0.05). The allele frequency analysis found no significant difference in the IL-17A rs2275913 polymorphism between case and control groups. However, the IL-17F rs763780 and IL-6 rs1800795 had significantly greater frequencies of specific alleles in the case group compared to the control group. The A-A haplotype of IL-17 in SNPs-rs 2,275,913 and rs763780 rising the risk of COVID-19 infection in PLWH by up to 2.398 times compared with the other haplotypes, and the A-G and G-A haplotypes have a protective role against the incidence of COVID-19 infection.</p><p><strong>Conclusion: </strong>This study is the first to show a significant correlation between the prevalence of COVID-19 and variety polymorphism at IL-17 and IL-6, which suggests that genetic changes in interleukin genes may relate to COVID-19 distribution.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"420"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143972244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioral cancer effects of ginger derivative 3-HDM exert oxidative stress-associated apoptosis and DNA damage.","authors":"Kuan-Liang Chen, Hsin-I Lu, Ching-Yu Yen, Chung-Yi Chen, Tsu-Ming Chien, Jiiang-Huei Jeng, Bing-Hung Chen, Hsueh-Wei Chang","doi":"10.1007/s11033-025-10514-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10514-2","url":null,"abstract":"<p><strong>Background: </strong>3-Hydroxy-1-(3',5'-dimethoxy-4'-hydroxy-phenyl)-hexan-5-one (3-HDM), a novel ginger Zingiber officinale-derived compound, lacks anti-cancer investigation, especially for oral cancer. This study addresses the antioral function and mechanism of 3-HDM against oral cancer cells (Ca9-22 and CAL 27).</p><p><strong>Method: </strong>MTS, flow cytometry, and western blotting were used to determine cell viability and antioral function and mechanism.</p><p><strong>Results: </strong>3-HDM inhibits oral cancer cell viability without normal cell (S-G) toxicity. This selective antiproliferation relies on oxidative stress validated by N-acetylcysteine (NAC), a reactive oxygen species (ROS) remover. 3-HDM upregulates subG1 and annexin V proportions, enhances caspases 3 and 8 activation to a greater extent in oral cancer than in normal cells, reverted by NAC. This process demonstrates the ROS-dependent selective apoptotic character of 3-HDM. 3-HDM also upregulates more ROS and mitochondrial superoxide and downregulates the mitochondrial membrane potential and glutathione in oral cancer than in normal cells in a ROS-dependent manner. Moreover, 3-HDM suppresses antioxidant signaling mRNA expressions such as NFE2L2, NQO1, and TXN and inhibits NFE2L2 phosphorylation in oral cancer cells compared to normal cells. NAC also downregulates the 3-HDM-induced γH2AX and 8-hydroxy-2-deoxyguanosine DNA damage markers.</p><p><strong>Conclusion: </strong>3-HDM shows selective antioral cancer effects and mechanisms without toxicity to normal cells via oxidative stress regulation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"414"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144044404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting hypoxia-related pathobiology in Alzheimer's disease: strategies for prevention and treatment.","authors":"Veerta Sharma, Reet Verma, Thakur Gurjeet Singh","doi":"10.1007/s11033-025-10520-4","DOIUrl":"https://doi.org/10.1007/s11033-025-10520-4","url":null,"abstract":"<p><strong>Introduction: </strong>Alzheimer's Disease (AD) is a neurodegenerative condition characterised by cognitive decline and memory impairment. Recent research highlights the important role of hypoxia, a state of insufficient oxygen availability, in exacerbating AD pathogenesis.</p><p><strong>Materials and methods: </strong>Through the use of a number of different search engines like Scopus, PubMed, Bentham, and Elsevier databases, a literature review was carried out for investigating the role of hypoxia mediated pathobiology in AD. Only peerreviewed articles published in reputable journals in English language were included. Conversely, non-peer-reviewed articles, conference abstracts, and editorials were excluded, along with studies lacking experimental or clinical relevance or those unavailable in full text.</p><p><strong>Conclusion: </strong>Hypoxia exacerbates core pathological features such as oxidative stress, neuroinflammation, mitochondrial dysfunction, amyloid-beta (Aβ) dysregulation, and hyperphosphorylation of tau protein. These interlinked mechanisms establish a self-perpetuating cycle of neuronal damage, accelerating disease progression. Addressing hypoxia as a modifiable risk factor offers potential for both prevention and treatment of AD. Exploring hypoxia and the HIF signalling pathway may help counteract the neuropathological and symptomatic effects of neurodegeneration.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"416"},"PeriodicalIF":2.6,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}