Molecular Biology Reports最新文献

{"title":"Neuroprotective capacity of Celastrus paniculatus on rotenone-induced parkinsonism in zebrafish model.","authors":"Nivedita Manoharan, Dheepthi Jayamurali, Anitha Sridhar, Sathya Narayanan Govindarajulu","doi":"10.1007/s11033-025-10384-8","DOIUrl":"https://doi.org/10.1007/s11033-025-10384-8","url":null,"abstract":"<p><strong>Introduction: </strong>Parkinson's disease, a neurodegenerative disorder, affects millions globally, with age, genetics, and environmental conditions increasing risk. Global burden could reach 12 million by 2050. To observe the effect of Celastrus paniculatus in rotenone-induced Parkinsonism in zebrafish model.</p><p><strong>Method: </strong>The fishes were divided into four groups and the experiment was carried out for 21days. Group I- Control; Group II- Rotenone induced (5 µg/L) dissolved in 0.1% DMSO; Group III - Aqueous extract of Celastrus paniculatus (CP) (20 µg/L) and Group IV - Rot + CP. After 21 days zebrafish was sacrificed and the brain was isolated for further analysis. The neurobehavioral studies were done using open field test, novel tank test and light and dark test, and the cognitive behavior using T-maze and customized fish maze. The antioxidant, neurotransmitter, mitochondrial assay and mRNA expressions were seen.</p><p><strong>Result: </strong>The rotenone has shown an increased freezing bout, decreased exploration of the tank and average speed has demonstrated motor impairment and also memory impairment was exhibited. There was elevated cortisol and LPO and reduced antioxidant status. The neurotransmitters changes and mitochondrial dysfunction were also observed. The study showed increased in α-synuclein and decreased in DJ1 and LRRK2 expressions. In the present study, the aqueous extract of CP has cognitive dysfunctions and improves memory. CP has also shown amelioration against the production of ROS, mitochondrial dysfunctions and DNA damages caused by rotenone.</p><p><strong>Conclusion: </strong>CP is known for its medicinal and pharmacological properties. CP has also shown to improve the cognitive dysfunction caused by rotenone and have showed an improvement in effect.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"272"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The complex role of glycine N-methyltransferase in metabolism-a review.","authors":"Md Suzauddula, Md Numan Islam, Tanvir Ahmed","doi":"10.1007/s11033-025-10374-w","DOIUrl":"https://doi.org/10.1007/s11033-025-10374-w","url":null,"abstract":"<p><strong>Background: </strong>Glycine N-methyltransferase (GNMT) is an enzyme predominantly found in the liver, playing a crucial role in various metabolic pathways. GNMT is involved in transmethylation, transsulfuration, one-carbon metabolism, energy metabolism, and DNA methylation. Deletion or Knockdown of GNMT influences the expression of several key metabolic enzymes by accumulating S-adenosylmethionine (SAM). Dysregulation of GNMT and these metabolic enzymes can lead to metabolic dysfunction and chronic diseases.</p><p><strong>Objective: </strong>To provide a comprehensive review of the impact of Glycine N-methyltransferase (GNMT) on metabolism, focusing on its epigenetic and genetic mechanisms, its role in metabolic pathways, and its association with chronic diseases.</p><p><strong>Results: </strong>GNMT is highly expressed in the liver and exerts direct and indirect effects on various metabolic pathways, including transmethylation, transsulfuration, one-carbon metabolism, energy metabolism, and global DNA methylation. Current understanding suggests that GNMT operates through both epigenetic and genetic mechanisms, influencing the expression of key metabolic enzymes such as BHMT, NNMT, PEMT, DNMTs, CBS, and MTHFR through the accumulation of S-adenosylmethionine. Dysregulation of these proteins not only affects metabolic function but also contributes to the development of several chronic diseases. Furthermore, the level of GNMT protein has been directly linked to non-alcoholic fatty liver disease, with its function being gender, age, and organ specific. At the same time, GNMT and disease progression correlate, dietary supplementation and pharmacological approaches have shown promise in controlling GNMT levels.</p><p><strong>Conclusion: </strong>GNMT plays a multifaceted role in metabolism, influencing various pathways and contributing to chronic disease development. Understanding its mechanisms and interactions opens avenues for targeted dietary and pharmacological therapies to manage GNMT-related metabolic dysfunction.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"271"},"PeriodicalIF":2.6,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143536937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering the anticancer potential of thymoquinone: in-depth exploration of the potent flavonoid from Nigella sativa.","authors":"Bunty Sharma, Himanshu Shekhar, Anidrisha Sahu, Shafiul Haque, Damandeep Kaur, Hardeep Singh Tuli, Ujjawal Sharma","doi":"10.1007/s11033-025-10375-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10375-9","url":null,"abstract":"<p><p>Since its first written description around 3000 BC until the present day, cancer has stood as a leading global cause of death, claiming the lives of 1 in 6 individuals. Due to its widespread impact and lethality, it remains one of the most explored yet most challenging disease for the global scientific community. Throughout history, various plant extracts have been used in treating numerous diseases, including cancer. These natural extracts are regaining attention due to their therapeutic benefits and lesser side effects. Thymoquinone, chemically 2-isopropyl-5-methylbenzo-1,4-quinone, constitutes the primary bioactive component of the plant Nigella sativa. Extensive research across in vivo, in vitro models, and clinical trials has revealed Thymoquinone's noteworthy therapeutic potential against cancer. Thymoquinone has shown promising anti-cancer activity in various cancers including breast cancer, lung cancer, prostate cancer, colorectal cancer, cervical cancer, pancreatic cancer, gastric cancer and blood cancers. However, there are challenges such as limited clinical trials, low bioavailability, and the need for more research to understand its long-term safety and effectiveness. This article provides a comprehensive and thorough review of thymoquinone, covering its effectiveness across various malignancies, the molecular signalling pathways it influences, and its role in triggering apoptosis and inhibiting inflammation, angiogenesis, and metastasis. Additionally, the review includes a thorough examination of thymoquinone's pharmacokinetics and safety, making it the first of its kind in its comprehensiveness.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"268"},"PeriodicalIF":2.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pharmacological approaches to enhance mitochondrial biogenesis: focus on PGC-1Α, AMPK, and SIRT1 in cellular health.","authors":"Ahmet Alperen Palabiyik, Esra Palabiyik","doi":"10.1007/s11033-025-10368-8","DOIUrl":"https://doi.org/10.1007/s11033-025-10368-8","url":null,"abstract":"<p><strong>Background: </strong>Mitochondrial biogenesis is essential for cellular energy balance and metabolic stability. Its dysregulation is linked to various metabolic and neurodegenerative diseases, making it a significant therapeutic target. Pharmacological approaches aimed at enhancing mitochondrial function have gained attention for their potential to restore cellular metabolism.</p><p><strong>Objectives: </strong>This review examines recent advancements in pharmacological strategies targeting mitochondrial biogenesis, focusing on the roles of PGC-1α, AMPK, and SIRT1, alongside novel therapeutic agents and drug delivery systems.</p><p><strong>Methods: </strong>A systematic review of studies published between 2018 and 2023 was conducted using databases such as PubMed, Web of Science, and Elsevier. Keywords related to mitochondrial biogenesis and pharmacological modulation were used to identify relevant literature.</p><p><strong>Results: </strong>Various pharmacological agents, including resveratrol, curcumin, and metformin, activate mitochondrial biogenesis through different pathways. SIRT1 activators and AMPK agonists have shown promise in improving mitochondrial function. Advances in mitochondria-targeted drug delivery systems enhance therapeutic efficacy, yet challenges remain in clinical translation due to the complexity of mitochondrial regulation.</p><p><strong>Conclusion: </strong>Pharmacological modulation of mitochondrial biogenesis holds therapeutic potential for metabolic and neurodegenerative diseases. While preclinical studies are promising, further research is needed to optimize drug efficacy, delivery methods, and personalized treatment strategies.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"270"},"PeriodicalIF":2.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"mir-188-5p emerges as an oncomir to promote chronic myeloid leukemia via upregulation of BUB3 and SUMO2.","authors":"Osman Akidan, Nina Petrovic, Sema Misir","doi":"10.1007/s11033-025-10359-9","DOIUrl":"https://doi.org/10.1007/s11033-025-10359-9","url":null,"abstract":"<p><strong>Background: </strong>Chronic myeloid leukemia (CML) is an aggressive malignancy originating from hematopoietic stem cells. miRNAs play a role in physiological and developmental processes, including cellular proliferation, apoptosis, angiogenesis, and differentiation, and in CML's prognosis, diagnosis, and treatment. This study aimed to investigate the function and possible mechanisms of action of miR-188-5p in the development and progression of chronic myeloid leukemia.</p><p><strong>Methods and results: </strong>miRNA expression profiles were obtained from the GSE90773 dataset in the Gene Expression Omnibus (GEO). GEO2R was used to identify differentially expressed miRNAs. miRNET, miRDB, CancerSEA, GeneMANIA, and BioGRID databases were applied to assess the biological function of miRNA and target molecules in CML. RT-PCR performed validation analyses of miRNA and target molecules in CML. To determine the power of miR-188-5p expression levels to distinguish patients with CML from control, the ROC analysis was performed. miR-188-5p is significantly increased in K-562 cells, and overexpression of miR-188-5p was associated with clinicopathological features. miR-188-5p showed significantly higher AUC values (AUC = 1.0, p = 0.0001). The cut-off of miR-188-5p was 6.74. miRDB and mirNET predicted BUB3 and SUMO2 as a potential target gene of miR-188-5p. Additionally, increased expression of BUB3 and SUMO2 was observed in the K-562 cell. Bub3 is implicated in apoptosis and the cell cycle, whereas Sumo2 protein sumoylation and DNA binding are believed to contribute to catabolic processes.</p><p><strong>Conclusions: </strong>Our results suggest that miR-188-5p acts as an oncomiRNA in CML pathogenesis and may be a promising therapeutic target for CML.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"269"},"PeriodicalIF":2.6,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143523780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide identification and expression analysis of the lipoxygenase gene family in sesame reveals regulatory networks in response to abiotic stress.","authors":"Tulsi, Ishwar Patidar, Dinakara Rao Ampasala","doi":"10.1007/s11033-025-10371-z","DOIUrl":"10.1007/s11033-025-10371-z","url":null,"abstract":"<p><strong>Background: </strong>Plant lipoxygenase (Lox) genes, catalyze polyunsaturated fatty acids and play essential roles in plant growth, development, and stress responses. It is extensively studied under various stresses, their role in abiotic stress responses remains unexplored in sesame.</p><p><strong>Methods and results: </strong>This study identified seven Lox genes in sesame divided into two subfamilies: 9-Lox (Silox1, Silox2 and Silox3) are likely involved in pathogen defence and signalling and 13-Lox (Type-I: Silox4 and Type-II: Silox5, Silox6 and Silox7) play crucial roles in jasmonic acid biosynthesis and abiotic stress responses. Silox genes have undergone purifying selection, promoting the stability of gene function and prefer codons with A or T in the third position. The chromosomal distribution, sequence similarity, and subcellular localization, with conserved lipoxygenase domains and motifs were analysed. Promoter regions contained 34 cis-acting regulatory elements (e.g. WRKY, ERF, and bHLH) and 35 transcription factors binding sites (TFBS) linked to light, stress (e.g. MYC, W-box, ERE and STRE), phytohormones, and growth. Differential Gene Expression (DGE) analysis showed Lox1 was upregulated in Drought sensitive (DS) and in Drought tolerant (DT) the Lox1 & Lox3 were upregulated when compared to control. In addition, weighted gene co-expression network analysis (WGCNA) of Lox, showed that blue module is positively correlated with drought tolerance. Fourteen hub genes related to stress were identified, which closely associated with Lox1. Gene ontology and KEGG pathway analyses showed that these genes were linked to linoleic acid metabolism, lipid metabolism, and stress response. Quantitative Real-Time PCR (qRT-PCR) analysis confirmed that Silox genes showed time-varying differential expression under drought, salt and a combined drought-salt stress treatments.</p><p><strong>Conclusion: </strong>This research lays the groundwork for future studies on the role of Lox genes in sesame's growth and stress adaptation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"266"},"PeriodicalIF":2.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprospecting of 101 facultative rumen bacterial isolates through comprehensive genome analysis.","authors":"Minal Bhure, Kaksha Savaliya, Sonal Patil, Chitra Nehra, Ramesh Pandit, Tejas Shah, Niteen V Patil, Ashutosh K Patel, Subhash Kachhawaha, Ram N Kumawat, Madhvi Joshi, Chaitanya G Joshi","doi":"10.1007/s11033-025-10291-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10291-y","url":null,"abstract":"<p><strong>Background: </strong>Microbes within the rumen play a pivotal role in the digestion of feed ingested by the ruminants. Researchers have been investigating microbes within rumen to assess its genetic capabilities, which hold immense potential across various fields including agro-industrial advantages. Since rumen is preliminary an anaerobic sac, numerous anaerobic bacteria and fungi have been isolated and characterized, however facultative anaerobic bacteria yet not fully investigated.</p><p><strong>Methods and results: </strong>In present study, we isolated, characterized and performed whole genome analysis of 101 facultative anaerobic bacteria from rumen, offering a unique perspective compared to metagenomic approaches. All assembled genomes were of high quality, i.e. completeness 100% (only seven were between 92 and 99.5%) and only two had contamination > 5%. We identified 9,542 sequences of Carbohydrate-Active Enzymes (CAZymes). Over 8,136 of these CAZymes were full-length sequences, with 2,048 harbouring signal peptides also. Xylan (n = 634), pectin (n = 604), and starch (n = 312) degrading enzyme sequences were dominant. Several isolates also harbour secondary metabolite biosynthesis gene clusters for various metabolites, including fengycin, lichenysin, bacillibactins, bacilysin etc. All the isolates have metabolic versatility, encompassing pathways such as carbohydrate, amino acid, lipid, and vitamin and cofactor metabolism. Intriguingly, lipoic acid metabolism was absent in most of these facultative bacterial isolates.</p><p><strong>Conclusion: </strong>This comprehensive study sheds light on the genetic potential of culturable facultative rumen bacteria, emphasizing their pivotal roles in carbohydrate degradation, secondary metabolite production, and metabolic diversity. These findings hold promise for enhancing ruminant nutrition, advancing eco-friendly biomass conversion, and bolstering bioprospecting of industrially important biocules and enzymes biofuel production.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"265"},"PeriodicalIF":2.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular mechanisms of Hippo pathway in tumorigenesis: therapeutic implications.","authors":"Mohamed J Saadh, Hanan Hassan Ahmed, Radhwan Abdul Kareem, Ashok Kumar Bishoyi, R Roopashree, Debasish Shit, Renu Arya, Abhishek Sharma, Kakhramon Khaitov, Hayder Naji Sameer, Ahmed Yaseen, Zainab H Athab, Mohaned Adil","doi":"10.1007/s11033-025-10372-y","DOIUrl":"https://doi.org/10.1007/s11033-025-10372-y","url":null,"abstract":"<p><p>The Hippo signaling pathway is a pivotal regulator of tissue homeostasis, organ size, and cell proliferation. Its dysregulation is profoundly implicated in various forms of cancer, making it a highly promising target for therapeutic intervention. This review extensively evaluates the mechanisms underlying the dysregulation of the Hippo pathway in cancer cells and the molecular processes linking these alterations to tumorigenesis. Under normal physiological conditions, the Hippo pathway is a guardian, ensuring controlled cellular proliferation and programmed cell death. However, numerous mutations and epigenetic modifications can disrupt this equilibrium in cancer cells, leading to unchecked cell proliferation, enhanced survival, and metastatic capabilities. The pathway's interaction with other critical signaling networks, including Wnt/β-catenin, PI3K/Akt, TGF-β/SMAD, and EGFR pathways, further amplifies its oncogenic potential. Central to these disruptions is the activation of YAP and TAZ transcriptional coactivators, which drive the expression of genes that promote oncogenesis. This review delves into the molecular mechanisms responsible for the dysregulation of the Hippo pathway in cancer, elucidating how these disruptions contribute to tumorigenesis. We also explore potential therapeutic strategies, including inhibitors targeting YAP/TAZ activity and modulators of upstream signaling components. Despite significant advancements in understanding the Hippo pathway's role in cancer, numerous questions remain unresolved. Continued research is imperative to unravel the complex interactions within this pathway and to develop innovative and effective therapies for clinical application. In conclusion, the comprehensive understanding of the Hippo pathway's regulatory mechanisms offers significant potential for advancing cancer therapies, regenerative medicine, and treatments for chronic diseases. The translation of these insights into clinical practice will necessitate collaborative efforts from researchers, clinicians, and pharmaceutical developers to bring novel and effective therapies to patients, ultimately improving clinical outcomes and advancing the field of oncology.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"267"},"PeriodicalIF":2.6,"publicationDate":"2025-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143516188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tracking genetic diversity in amur tigers: a long-term study using microsatellites in Southwest Primorye, Russia.","authors":"Jangmi Lee, Taisiia Marchenkova, Dina Matiukhina, Anya Lim, Yung Kun Kim, Daecheol Jeong, Jee Yun Hyun, Sujoo Cho, Dong Youn Kim, Ying Li, Yury Darman, Mi-Sook Min, Je-Yeol Cho, Victor Bardyuk, Younghee Lee, Puneet Pandey, Hang Lee","doi":"10.1007/s11033-025-10339-z","DOIUrl":"10.1007/s11033-025-10339-z","url":null,"abstract":"<p><strong>Background: </strong>The tiger population in Southwest Primorye is small and predominantly isolated from the main Sikhote-Alin population, which constitutes approximately 90% of the wild Amur tiger population. By 1996, this population declined to fewer than 10 individuals, but it has since grown and expanded into nearby habitats, now numbering over 50 individuals. Therefore, the regular genetic monitoring of this population is essential, as it has grown from a few founding members and remained geographically isolated.</p><p><strong>Methods and results: </strong>Genetic diversity was assessed using nine heterologous microsatellite markers amplified from non-invasively collected samples of 20 individual tigers. The Southwest Primorye tiger population exhibited moderate genetic diversity, with allelic richness (Na) at 3.67 and observed heterozygosity (Ho) at 0.63. Additionally, we detected a slight tendency toward heterozygosity excess at several loci, with an overall negative FIS, which may be influenced by recent genetic admixture or subtle population structuring. comparative assessment between our study and Sugimoto et al. (2012) revealed a marginal increase in genetic diversity over time, suggesting improved genetic health of the population, potentially due to genetic exchange with other populations.</p><p><strong>Conclusions: </strong>The significant growth and expansion of the Southwest Primorye tiger population into adjacent areas of Northeast China over the past two decades suggest a positive population trajectory. This modest increase in genetic diversity indicates a potentially favorable population condition. However, continuous genetic monitoring remains essential to track genetic trends, detect potential risks, and inform conservation strategies. This study highlights the need for ongoing evaluations to ensure the long-term survival of the Amur tiger population in Southwest Primorye.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"264"},"PeriodicalIF":2.6,"publicationDate":"2025-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11865129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143502539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"XadA-like adhesin XADA2 regulates biofilm formation in X. fastidiosa subsp. fastidiosa putatively by engaging oleic-acid derived oxylipins.","authors":"Valeria Scala, Manuel Salustri, Marcus Vinicius Merfa, Marzia Beccaccioli, Leonardo Lascala, Leonardo De La Fuente, Massimo Reverberi","doi":"10.1007/s11033-025-10259-y","DOIUrl":"10.1007/s11033-025-10259-y","url":null,"abstract":"<p><strong>Background: </strong>The oxylipins 10-HpOME and 7,10-DiHoME derive from oleic acid and have been extensively studied for their ability to regulate contractions, microcolony formation and biofilm formation in the model organism Pseudomonas aeruginosa.</p><p><strong>Methods and results: </strong>Xylella fastidiosa subsp. pauca strain de Donno has been reported to produce 10-HpOME and 7,10-DiHOME in vivo when inoculated in the model plant Nicotiana tabacum or in naturally occurring infected olive trees. In this study, we deciphered the relationship among cell adhesion and oxylipins in Xylella fastidiosa subsp. fastidiosa (Temecula1 strain) and subsp. multiplex (AlmaEM3 strain). The role of the PD0744 gene, encoding for XadA2, a non-fimbrial adhesin belonging to the trimeric autotransporter family, probably involved in the surface attachment required in the initial phase of biofilm formation was investigated. PD0744 deletion mutants in two X. fastidiosa strains were generated, through homologous recombination, and the impact of its deletion on bacterial lifestyle was assessed. In vitro assays were performed to characterize the mutant phenotype, particularly in twitching motility and its capability to grow and form biofilm. Mutants showed a reduced twitching motility and biofilm formation compared to wild type strains. HPLC-MS/MS analysis revealed a decrease in 7,10-DiHOME production together with an increase of its precursor 10-HpOME in the mutants.</p><p><strong>Conclusions: </strong>7,10-DiHOME could be a crucial signaling molecule to promote biofilm formation and twitching motility, whose synthesis likely depends on a signal transduction requiring the presence of the adhesin XadA2 and thus not working if this protein is depleted. These results help understanding the complex regulation of biofilm formation in this devastating pathogen.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"263"},"PeriodicalIF":2.6,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11861136/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143492916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}