Molecular Biology Reports最新文献

{"title":"A chromosome-anchored reference assembly for the gray snapper, Lutjanus griseus.","authors":"Tami Hildahl, Eric Saillant","doi":"10.1007/s11033-024-10195-3","DOIUrl":"https://doi.org/10.1007/s11033-024-10195-3","url":null,"abstract":"<p><strong>Background: </strong>The gray snapper (Lutjanus griseus) is a marine reef fish commonly found in coastal and shelf waters of the tropical and subtropical western Atlantic Ocean. In this work, a draft reference genome was developed to support population genomic studies of gray snapper needed to assist with conservation and fisheries management efforts.</p><p><strong>Methods and results: </strong>Hybrid assembly of PacBio and Illumina sequencing reads yielded a 1,003,098,032 bp reference across 2039 scaffolds with N50 and L50 values of 1,691,591 bp and 163 scaffolds, respectively. A linkage map was generated by genotyping parents and 286 offspring of a single pair cross using the double digest Restriction Associated DNA (ddRAD) protocol. The map featured 10,965 informative markers that were assigned to 24 linkage groups and used to scaffold the assembly. The anchored assembly spanned 962,844,722 bp (N50 = 41,865,368 bp, L50 = 11 scaffolds) in 24 pseudo chromosomes and yielded a BUSCO score of 95.2%. Annotation of the final assembly in Augustus revealed 39,070 candidate genes. Gene ontology annotation was obtained for 48.8% of the predicted genes. Analysis of shared syntenic regions revealed that each gray snapper chromosome matched a unique Japanese medaka, Oryzias latipes, counterpart and the reference showed a high degree of synteny with the closely related Lutjanus erythropterus assembly.</p><p><strong>Conclusions: </strong>This resource will greatly enhance genomic studies of conservation and management of natural populations as well as efforts to develop breeding programs for this species and other lutjanids.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"99"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mass-spectrometry-based quantitative proteomic analysis reveals that methylglyoxal and carnosine influence oxidative stress and RNA-processing associated proteins in renal proximal tubule epithelial cells.","authors":"Lei Liu, Shiqi Zhang, Juan Xu, Yadi Cao, Di Cui, Chao Liu, Bing Shen, Yonggui Wu, Qiu Zhang","doi":"10.1007/s11033-024-10190-8","DOIUrl":"https://doi.org/10.1007/s11033-024-10190-8","url":null,"abstract":"<p><strong>Background: </strong>Tubular injury triggered by hyperglycemia is an important pathological characteristic in diabetic nephropathy (DN). Accumulated advanced glycation end products and their precursor methylglyoxal (MGO), contribute to the development of DN. Carnosine has been shown to prevent the development of DN but the underlying mechanism still needs to be studied in depth. In this study, we explored the potential proteins influenced by MGO and carnosine in tubule epithelial cells.</p><p><strong>Methods and results: </strong>HK-2 cells were treated with MGO, carnosine, or a combination. Differentially expressed proteins (DEPs) between different groups were identified by isobaric tag for relative and absolute quantitation-based mass spectrometry. In the comparison between MGO and control, 29 DEPs were found to be associated with antioxidation and RNA methylation. In the comparison between carnosine and control, 10 DEPs were associated with ubiquitin protein ligase activity and RNA metabolism. In the comparison between MGO + carnosine and MGO, carnosine-induced DEPs in the presence of MGO were mainly related to RNA splicing and mRNA processing. MGO effects on OSTC expression was inversely correlated with that of carnosine. Some DEPs (OSTC, PRDX5, NEDD4L, NOP2, TRMT6, and GEMIN2) were validated by Western blotting. Additional experiments showed the 28 kD particle of Smith antigen was also influenced by MGO and carnosine.</p><p><strong>Conclusions: </strong>Carnosine can influence RNA processing and spliceosome-related proteins, and change MGO's effect on HK-2 cells. This study helps to understand the mechanism by which MGO contributes to the development of DN and promotes further identification of carnosine downstream proteins as therapeutic targets for DN.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"100"},"PeriodicalIF":2.6,"publicationDate":"2025-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling exogenous potential of phytohormones as sustainable arsenals against plant pathogens: molecular signaling and crosstalk insights.","authors":"Lucky Duhan, Ritu Pasrija","doi":"10.1007/s11033-024-10206-3","DOIUrl":"https://doi.org/10.1007/s11033-024-10206-3","url":null,"abstract":"<p><p>Plants frequently confront pathogens that disrupt physiological and molecular functions, ultimately reducing agricultural yields. To counter these challenges, plants activate sophisticated defense mechanisms to recognize stress signals while optimizing growth. Phytohormones signaling pathways and their crosstalk are central to regulating plant growth, development and defense. Numerous proteins associated with phytohormone signaling pathways have been identified, including receptors for several vital hormones. Previous studies indicate that defense phytohormones, like salicylic acid (SA), jasmonic acid (JA) and ethylene (ET), are crucial to pathogen defense. SA specifically mediates systemic acquired resistance against biotrophic pathogens, while induced systemic resistance relies on JA and ET signaling in response to necrotrophic pathogens. Other hormones, typically associated with growth and development, such as ethylene, abscisic acid, brassinosteroids, melatonin, gibberellins, auxin, and cytokinin, also interact in a complex network of synergistic and antagonistic relationships with defense phytohormones. Moreover, they can achieve effects that surpass conventional pathogen control methods, suggesting their potential as exogenous biocontrol agents. During the past decade, our knowledge of hormone signaling and stress response has become immense. Thus, this review is an attempt to summarize some of the advances in plant signaling and crosstalk mechanisms as well as their potential to be a future arsenal in biotic stress mitigation strategies. Ultimately, this work emphasizes using exogenous phytohormones as a viable alternative for controlling pathogens to enhance crop productivity in pathogen-affected regions.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"98"},"PeriodicalIF":2.6,"publicationDate":"2025-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142922097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artemisinin's molecular symphony: illuminating pathways for cancer therapy.","authors":"Sandeep Kumar Jhade, Karthik Kalidoss, Poonam Kumari Pathak, Rahul Shrivastava","doi":"10.1007/s11033-024-10202-7","DOIUrl":"https://doi.org/10.1007/s11033-024-10202-7","url":null,"abstract":"<p><p>Artemisinin (ART), a sesquiterpene lactone derived from the sweet wormwood plant (Artemisia annua), exhibits potent anti-malarial and anti-microbial properties, with emerging evidence suggesting its anticancer potential. This review delves into the molecular intricacies underlying ART's anticancer effects, elucidating its modulation of cell signaling pathways, induction of apoptosis and autophagy, and inhibition of angiogenesis crucial for cancer progression. Additionally, the review highlights ART's impact on oxidative stress and DNA damage within cancer cells, along with its potential synergistic effects with conventional cancer drugs to mitigate side effects. Despite notable strides, further elucidation of ART's mechanisms and clinical validation across diverse cancer types are necessary. Conclusively, this review provides a brief overview of the molecular foundation that makes ART a promising candidate for future cancer therapeutic strategies and emphasises the need for further research to fully comprehend the molecular complexity of ART-mediated cancer therapies.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"95"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142910047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sirtuin 1 mediates the pro-survival effects of vitamin D in angiotensin II-induced hypertrophy of H9c2 cardiomyoblasts.","authors":"Akram Astani, Abdulbaset Maroofi, Seyedhossein Hekmatimoghaddam, Mohammadtaghi Sarebanhassanabadi, Fatemeh Safari","doi":"10.1007/s11033-024-10168-6","DOIUrl":"https://doi.org/10.1007/s11033-024-10168-6","url":null,"abstract":"<p><strong>Background: </strong>The role of 1,25-dihydroxyvitamin-D3 (VitD) and sirtuin-1 (SIRT1) in mitigating pathological cardiac remodeling is well recognized. However, the potential for SIRT1 to mediate the inhibitory effects of VitD on angiotensin II (Ang II) -induced hypertrophy in H9c2 cardiomyoblasts remains unclear.</p><p><strong>Methods: </strong>H9c2 cardiomyoblasts were exposed to Ang II or a combination of VitD and Ang II, both in the absence and presence of SIRT1-specific siRNA. In each cell group, cell viability, hypertrophy, and redox state were evaluated using relevant techniques.</p><p><strong>Results: </strong>In H9c2 cells transfected with SIRT1 siRNA, VitD failed to significantly counteract the Ang II-induced perturbations, which included a reduction in cell viability, decreased CAT and SOD activity/mRNA levels, diminished MnSOD mRNA levels, and increased MDA content. Conversely, VitD significantly inhibited Ang II-induced hypertrophy in H9c2 cells by reducing cell size and lowering ANP and BNP mRNA levels, regardless of SIRT1 status. Notably, neither Ang II nor VitD altered the expression of SIRT1 mRNA or protein in H9c2 cells.</p><p><strong>Conclusion: </strong>SIRT1 serves as an important regulator of pro-survival, but not anti-hypertrophic functions of VitD in hypertrophied cardiomyoblasts. Indeed, the absence of SIRT1 jeopardizes the capabilities of VitD to confer its pro-survival activity in H9c2 cells. Therefore, SIRT1-centered activating compounds may augment the protective effects of VitD, providing a promising therapeutic strategy to reduce the risk of cardiac hypertrophy and heart failure.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"96"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909923","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bio-consortia of Bacillus spp. induces defense gene expression and suppresses Orthotospovirus arachinecrosis in tomato.","authors":"S Harish, M Nivedha, C Sangeetha, P S Saranya, K Saravanakumari, K Anandhi","doi":"10.1007/s11033-024-10185-5","DOIUrl":"https://doi.org/10.1007/s11033-024-10185-5","url":null,"abstract":"<p><strong>Background: </strong>Tomato (Solanum lycopersicum L) is affected by various diseases among which Orthotospovirus arachinecrosis cause huge economical loss to the farmers. Management of viral diseases using systemic insecticides will target the beneficial microflora and fauna besides polluting the environment and cause health hazards. In this context, inducing systemic resistance (ISR) through Bacillus spp. is a promising alternative method which is natural, eco-friendly and safe, offering resistance against the disease.</p><p><strong>Methods and results: </strong>In the present study, eight Bacillus spp. has been tested for their anti-viral efficacy against O. arachinecrosis in tomato. Pre-inoculation spray of the consortium of Bacillus subtilis BST8, Bacillus subtilis EBPBS-4 and Bacillus subtilis Bbv57 as seed treatment (10 ml/kg), soil drenching (1%) and foliar application (0.5%) exhibited less disease severity index of 12.92 compared to inoculated control (85.98) in tomato in pot culture. Field experiments revealed that, sequential application of Bacillus spp. consortia (Bbv57 + BST8 + EBPBS4) formulation as seed treatment, (10 ml/kg), soil application (2 lit/acre) along with foliar spray (1 lit/acre) at 30 DAT, 45 DAT and 60 DAT recorded the lowest disease incidence over control. Defense gene expression studies through real-time PCR analysis revealed that there is differential expression of defense genes viz., PR1, PR2, PR3, NPR1, MAPKK and PAL during the tripartite interaction of Bacillus spp. with O. arachinecrosis in tomato.</p><p><strong>Conclusion: </strong>It is concluded that, consortia of Bacillus spp. induce systemic resistance in the plant besides promoting plant growth which can be utilized for the management of O. arachinecrosis infecting tomato.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"94"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of a biallelic MMUT variant (p.Thr230Arg) and its global perspective on clinical management.","authors":"Sumreena Mansoor, Qamar Ali, Sabeen Abid Khan, Munir Iqbal Malik, Muhammad Imran, Raheel Qamar, Maleeha Azam","doi":"10.1007/s11033-024-10194-4","DOIUrl":"https://doi.org/10.1007/s11033-024-10194-4","url":null,"abstract":"<p><strong>Background: </strong>Methylmalonic acidemia (MMA), type mut (0) is a rare type of genetic inborn error of metabolism (IEM) that is caused by aberrant malonyl-CoA mutase activity. Diagnosing IEM can be challenging due to its inherited onset and varying degrees of severity.</p><p><strong>Methods and results: </strong>In the present study, a consanguineous Pakistani family suspected of IEM was genetically analyzed using whole exome sequencing. A biallelic variant c.689 C > G (p.Thr230Arg) in MMUT was identified to be the causative factor of the disease, which helped in establishing the accurate diagnosis in the family to be MMA mut(0) type. On the basis of the genetic findings, the patient's condition was appropriately managed through a supportive nutrition plan and administration of oral L-carnitine.</p><p><strong>Conclusions: </strong>Identification of MMUT mutation through whole exome sequencing was helpful in solving the family and devising targeted management strategies. This study highlights the utility of genetic analysis in diagnosing and treating metabolic disorders like MMA in Pakistani inbred population.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"97"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Environmental influence on single methylation variation sites (SMVs) in the large yellow croaker (Larimichthys crocea): identification and correlation analysis.","authors":"Lihua Jiang, Yongqi Guo, Tianyu Li, Weihua Song, Shun Chen, Yifan Liu, Jialang Zheng","doi":"10.1007/s11033-024-10154-y","DOIUrl":"https://doi.org/10.1007/s11033-024-10154-y","url":null,"abstract":"<p><strong>Background: </strong>Larimichthys crocea is an important aquaculture species along the southeastern coast of China, with diverse environment and farming practices since artificial breeding, these different aquatic habitats are subject to significant variations in environmental factors that may involve modulation of gene expression through epigenetic mechanisms to enable species to survive and reproduce.</p><p><strong>Methods and results: </strong>This study aimed to identify methylation variation sites (SMVs) in different sequence contexts (CG, CHG, and CHH) within populations of L. crocea in different habitats. All SMV sites were subjected to linear regression with environmental factors to identify candidate genes involved environmental stress. The results indicate a significant correlation between SMV sites and various environmental factors. For the wild populations in Jinmen and Zhanjiang, the primary environmental pressures for adapting are temperature and salinity. In contrast, for the domesticated populations in Zhoushan and farmed population in Xiangshan, the main environmental pressures are nitrate and dissolved oxygen. Furthermore, genes related to temperature adaptation in different aquatic environments were identified, including nr3c2, igf1, hsp70, trpm3, and fgf1. The gene rasa3 was found to be associated with pH adaptation, while genes such as atp6ap1lb, slc15a4, and gpr39 were linked to salinity, ammonia nitrogen, and dissolved oxygen. Research on the association between single methylation variation sites (SMVs) and environmental factors in aquatic organisms is scarce.</p><p><strong>Conclusions: </strong>These results suggest that selection pressures can influence a significant proportion of methylation sites in this species, indirectly implying that epigenetic variation is not solely attributed to patterns of genetic variation, but is also closely linked to environmental differences. These results highlight the complex interactions between epigenetic regulation and environmental influences. Hence, this study provides preliminary evidence for a new perspective on the role of methylation patterns in L. crocea in environmental adaptation.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"93"},"PeriodicalIF":2.6,"publicationDate":"2024-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First microsatellite markers for the Amazonian species Copaifera multijuga Hayne developed using next-generation sequencing.","authors":"Rayssa Gomes Vasconcelos, Sandra Marcela Hernández-Rangel, Izeni Pires Farias, Tomas Hrbek, Paulo de Tarso Barbosa Sampaio, Raquel da Silva Medeiros, Antonio Saulo Cunha-Machado","doi":"10.1007/s11033-024-10192-6","DOIUrl":"https://doi.org/10.1007/s11033-024-10192-6","url":null,"abstract":"<p><strong>Background: </strong>Native to the Amazon region, Copaifera multijuga Hayne is a large tree (≈ 36 m in height) that is heavily exploited for extraction of its oleoresin. Many studies have addressed the phytochemical properties and applications of this raw material; however, there are few initiatives that have focused on the genetic characterization of native populations of this species. To this end, our objective was to develop microsatellite markers for C. multijuga, which were previously unavailable, and apply them to the characterization of a native population located in the Adolpho Ducke Forest Reserve (Manaus, Amazonas, Brazil).</p><p><strong>Methods and results: </strong>Using next-generation sequencing technology on the Ion Torrent PGM™ platform, 19 pairs of microsatellite primers were designed and developed. For the characterization and validation, we used a group of 47 C. multijuga trees. After preliminary tests, amplification conditions were standardized for 14 loci. The CmH05 locus was excluded from the analyses for being monomorphic, and the remaining loci were used to estimate key genetic parameters for the species, such as observed (Ho) and expected heterozygosity (He), total number of alleles (A), fixation index (f), and polymorphic information content (PIC), among others. The population showed levels of genetic diversity that were higher than 0.63 (Ho: 0.67; He: 0.64) and an average number of 5.4 alleles. According to the PIC estimates, all loci were considered highly (9) or moderately informative (3), except for CmH11, which had a value that was below 0.2.</p><p><strong>Conclusions: </strong>The 13 developed microsatellites were efficient in characterizing the genetic diversity of C. multijuga and may be advantageous in future investigations aimed at defining effective conservation strategies for the species.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"92"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterizing tick diversity among caprine hosts of Kerala, India: a phylogenetic study.","authors":"Sasi Adithya, Andrews Megha, Y Ajith, K S Athira, Anna-Maria Ettel, S Gireesh Sangeetha, Siju Susan Jacob, P V Tresamol","doi":"10.1007/s11033-024-10191-7","DOIUrl":"https://doi.org/10.1007/s11033-024-10191-7","url":null,"abstract":"<p><strong>Background: </strong>Ticks are prominent vectors of numerous pathogens that adversely affect human and animal health. Monitoring tick population dynamics is key in developing ideal tick-borne disease surveillance systems and critical vector control programmes. This study aimed to conduct the morphological and molecular characterization of ticks infesting domesticated goats in Kerala, India.</p><p><strong>Methods and results: </strong>A total of 30 goats presented to the small ruminant unit of the Teaching Veterinary Clinical Complex (TVCC), Mannuthy were randomly screened for tick infestation, with 22 (73.3%) found to be infested. Morphological identification of different life cycle stages and genera was conducted first, utilizing documented external characteristics such as body size, presence of eyes, and other key morphological traits. A total of 153 tick samples were collected from goats, and their identification revealed that they belonged to the genera Haemaphysalis and Rhipicephalus. This was followed by molecular analysis through sequencing a fragment of the cytochrome c oxidase subunit I (COXI) gene, a standard marker for tick identification. The results from molecular and phylogenetic analyses confirmed the tick species as Haemaphysalis bispinosa, H. intermedia, Rhipicephalus haemaphysaloides, and R. sanguineus. The sequenced specimens were deposited in the NCBI GenBank contributing to the global understanding of tick distribution and diversity in goats. The GenBank accession no. (s) of the isolates are PQ433166 (H. bispinosa), PQ433290 (H. intermedia), PQ433525 (R. haemaphysaloides), and PQ433586 (R. sanguineus).</p><p><strong>Conclusions: </strong>The findings of this study contribute to a better understanding of the tick fauna infesting goats in the region and emphasize the importance of developing research and monitoring plans to address the challenges posed by these ectoparasites. It also highlights a critical area for future study, targeting the vector potential of these arthropods in hemoparasitic diseases and zoonotic disease transmission.</p>","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"89"},"PeriodicalIF":2.6,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142909988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}