Role of Interleukin 37 and its polymorphism (rs3811047) in children with type I diabetes.

IF 2.8 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular Biology Reports Pub Date : 2025-07-07 DOI:10.1007/s11033-025-10779-7

Amany M Shabaan, Doaa A Gaber, E L Shaimaa Gomaa Ali, Rehab G Abd El-Hamid, Omayma O Abdelaleem

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引用次数: 0

Abstract

Background: Type 1 diabetes (T1D) is a multifaceted autoimmune condition. Interleukin-37 (IL-37), a cytokine from the IL-1 family, is recognized for its anti-inflammatory effects and its involvement in the progression of various autoimmune diseases (ADs). Despite this, the exact relationships are not fully clear. The objective of this study was to assess serum IL-37 levels and the polymorphism (rs3811047) to investigate its possible role in T1D.

Methods: The study involved 46 individuals with Type 1 Diabetes (T1D) and 45 healthy controls. Serum IL-37 levels were quantified using enzyme-linked immunosorbent assay (ELISA). Genomic DNA was extracted with a specialized DNA extraction kit. The specific single nucleotide polymorphism (SNP) in the IL-37 gene (rs3811047) was then genotyped using real-time polymerase chain reaction (RT-PCR).

Results: The serum concentrations of IL-37 did not show a significant difference between individuals with T1D and the control group, with a P-value of 0.7. However, our results revealed a significant correlation between T1D and the IL-37 polymorphism (rs3811047). Both the AG and GG genotypes were strongly associated with an elevated risk of developing T1D in comparison to the control group. Furthermore, the frequency of the G allele in rs3811047 was notably higher in the T1D group (56.5%) compared to the control group (32.2%).

Conclusions: These findings suggest that the rs3811047 variant in the IL-37 gene is a significant genetic marker linked to an increased susceptibility to T1D. The AG and GG genotypes, along with the G allele, are associated with a higher risk.

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白细胞介素37及其多态性（rs3811047）在儿童1型糖尿病中的作用

背景：1型糖尿病（T1D）是一种多方面的自身免疫性疾病。白细胞介素-37 （IL-37）是IL-1家族的一种细胞因子，因其抗炎作用和参与各种自身免疫性疾病（ADs）的进展而被公认。尽管如此，确切的关系还不完全清楚。本研究的目的是评估血清IL-37水平及其多态性（rs3811047），以探讨其在T1D中的可能作用。方法：研究纳入46例1型糖尿病患者和45例健康对照。采用酶联免疫吸附法（ELISA）定量测定血清IL-37水平。用专门的DNA提取试剂盒提取基因组DNA。利用实时聚合酶链反应（RT-PCR）对IL-37基因（rs3811047）特异性单核苷酸多态性（SNP）进行基因分型。结果：T1D患者与对照组血清IL-37浓度差异无统计学意义，p值为0.7。然而，我们的研究结果揭示了T1D与IL-37多态性（rs3811047）之间的显著相关性。与对照组相比，AG和GG基因型与发生T1D的风险升高密切相关。此外，T1D组rs3811047中G等位基因的频率（56.5%）显著高于对照组（32.2%）。结论：这些发现提示IL-37基因rs3811047变异是与T1D易感性增加相关的重要遗传标记。AG和GG基因型以及G等位基因与较高的风险相关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Biology Reports 生物-生化与分子生物学

CiteScore

5.00

自引率

0.00%

发文量

1048

审稿时长

5.6 months

期刊介绍： Molecular Biology Reports publishes original research papers and review articles that demonstrate novel molecular and cellular findings in both eukaryotes (animals, plants, algae, funghi) and prokaryotes (bacteria and archaea).The journal publishes results of both fundamental and translational research as well as new techniques that advance experimental progress in the field and presents original research papers, short communications and (mini-) reviews.