Molecular Biology Reports最新文献_第9页

The role of calcium ions and the transient receptor potential vanilloid (TRPV) channel in bone remodelling and orthodontic tooth movement.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-10 DOI: 10.1007/s11033-025-10399-1

Haoyu Li, Ruofang Zhang

{"title":"The role of calcium ions and the transient receptor potential vanilloid (TRPV) channel in bone remodelling and orthodontic tooth movement.","authors":"Haoyu Li, Ruofang Zhang","doi":"10.1007/s11033-025-10399-1","DOIUrl":"https://doi.org/10.1007/s11033-025-10399-1","url":null,"abstract":"During orthodontic treatment, the application of orthodontic forces to the periodontal tissues leads to the activation of osteoblasts and osteoclasts, which in turn induces bone remodelling and tooth movement. Calcium is a biologically essential element that exists in the internal environment and cells as calcium ions(Ca2+). The concentration of extracellular Ca2+ can affect the activity and function of osteoblasts and osteoclasts, as well as regulate bone remodelling. In the cell, calcium ions play a crucial role in cell signal transduction, acting as a second messenger. The orthodontic force increases intracellular Ca2+ concentration through a series of cascade reactions that affect the differentiation and apoptosis of osteoblasts and osteoclasts. Calcium channels on the cell membrane are crucial for intracellular and extracellular calcium transport. Transient Receptor Potential Vanilloid (TRPV) is a calcium ion permeable and mechanosensitive receptor comprising six calcium channel subtypes, TRPV1-6. This review will focus on the crucial role of Ca2+ in bone metabolism and provide a comprehensive description of the function and mechanism of each specific TRPV channel subtype in orthodontic tooth movement and bone remodelling.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"297"},"PeriodicalIF":2.6,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of XRCC1 (rs1799782) and XPD (rs13181) gene polymorphisms with renal failure risk in a sample of Iraqi population: a case-control study.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-08 DOI: 10.1007/s11033-025-10408-3

Fahad D F Abo-Ghneim, Dhafer A F Al-Koofee, Hussain Jasem Mohammed

{"title":"Association of XRCC1 (rs1799782) and XPD (rs13181) gene polymorphisms with renal failure risk in a sample of Iraqi population: a case-control study.","authors":"Fahad D F Abo-Ghneim, Dhafer A F Al-Koofee, Hussain Jasem Mohammed","doi":"10.1007/s11033-025-10408-3","DOIUrl":"10.1007/s11033-025-10408-3","url":null,"abstract":"Background: End-stage chronic kidney disease (CKD) can lead to life-threatening complications and is caused primarily by CKD and cardiovascular issues. CKD is characterized by the inability of the kidneys to filter waste and excess fluids from the blood. This study investigated the associations of the genetic variants XRCC1 rs1799782 (C194T) and ERCC2/XPD rs25487 (Q399R) with CKD susceptibility in Iraqi patients and related biochemical changes.Methods: The research was performed from 25/01/2023 to 30/06/2023, we analyzed the genetic associations of two SNPs of DNA repair genes (XRCC1 and ERCC2/XPD) in a case‒control study involving 219 CKD patients diagnosed by a nephrologist and 246 healthy controls. Data and blood samples were collected, and the genotype distribution frequency was determined via the PCR-based high-resolution melting (PCR-HRM) technique.Results: This study included 465 participants, with 219 CKD patients and 246 healthy controls. XRCC1 and ERCC2/XPD gene polymorphisms were significantly associated with CKD susceptibility in Iraqi patients (p = 0.025 and p = 0.0001, respectively). Multivariate linear regression confirmed the associations of rs1799782 G/A and rs13181T/G with CKD, adjusting for sex, age, and BMI. Moderate and statistically significant linkage disequilibrium (0.43) between the two SNPs was observed, indicating nonrandom associations.Conclusion: XRCC1 (rs1799782) and ERCC2/XPD (rs13181) polymorphisms are associated with an increased risk of CKD. The AG haplotype model is particularly related to increased CKD susceptibility in Iraqi patients, suggesting the importance of these DNA repair gene polymorphisms in CKD risk assessment.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"294"},"PeriodicalIF":2.6,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-transgenic RNAi strategies for sustainable plant viral disease control: a review.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-08 DOI: 10.1007/s11033-025-10376-8

Greeshma Varghese, Puja Dey, Munmi Borah

引用次数: 0

Dead-End protein expression, function, and mutation in cancer: a systematic review.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-07 DOI: 10.1007/s11033-025-10325-5

Homa Faraji, Farnaz Banakar, Arash Sadri, Azadeh Ebrahim-Habibi

{"title":"Dead-End protein expression, function, and mutation in cancer: a systematic review.","authors":"Homa Faraji, Farnaz Banakar, Arash Sadri, Azadeh Ebrahim-Habibi","doi":"10.1007/s11033-025-10325-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10325-5","url":null,"abstract":"Cancer incidence is rising globally, particularly in aging populations. Understanding the genetic, cellular, and molecular mechanisms underlying cancer is crucial for developing effective interventions. Dead-end protein 1 (DND1), an RNA-binding protein, plays a pivotal role in germ cell regulation and tumorigenesis. This systematic review investigates DND1's multifaceted roles in cancer progression and evaluates its interactions and potential as a therapeutic target. A systematic search of four databases (Web of Science, MEDLINE via PubMed, Scopus, and Embase) yielded 436 unique records. After screening, 38 studies were included for data extraction. STRING-based network analysis identified key interactors-including NANOS1-3, TDRD7, DAZL, and EIF2S2- and pathways associated with RNA binding, translational regulation, and apoptosis. DND1 demonstrates dual, context-dependent roles as both a tumor suppressor and promoter. Its regulation of miRNAs and interaction with germ cell-specific proteins emerged as critical mechanisms in tumor suppression and progression. This study highlights DND1's central role in cancer biology, with significant implications for diagnostics and therapeutics. The findings provide a robust foundation for experimental validation of key interactions and further exploration of DND1's molecular mechanisms. The dual functionality of DND1 as a tumor suppressor and promoter underscores its potential as a target for novel cancer therapies, particularly for germ cell tumors and other cancers.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"291"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial DNA variation and intervertebral disc degeneration: a genotypic analysis in a South African cohort.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-07 DOI: 10.1007/s11033-025-10394-6

Megan Collins, Brendon Pearce

{"title":"Mitochondrial DNA variation and intervertebral disc degeneration: a genotypic analysis in a South African cohort.","authors":"Megan Collins, Brendon Pearce","doi":"10.1007/s11033-025-10394-6","DOIUrl":"10.1007/s11033-025-10394-6","url":null,"abstract":"Background: Non-communicable diseases are multifactorial in that they can be caused by genetic factors, age, sex and poor lifestyle choices. They are estimated to account for 71% of deaths globally with 80% of these deaths occurring in low- and middle-income countries. This is particularly true for Intervertebral Disc Degeneration associated with mitochondrial dysfunction. Interestingly, mitochondrial dysfunction can arise from mutations in both the nuclear and the mitochondrial genomes. The present study, therefore, aimed to determine if there is an association between mitochondrial DNA mutations associated with mitochondrial dysfunction and disc degeneration in a South African cohort, and in addition, generate genetic data for understudied mutations in African populations.Methods and results: Mutations were selected using a systematic literature review. DNA was collected using buccal swabs and extracted using a standard salt-lysis protocol. Mass-array genotyping was done for previously reported as well as novel mutations. GenAlEx (version 6.5), RStudio and SHEsis were used for statistical analyses. Although no significant associations were found, the identified polymorphic mutations C16223T, A10398G and A8536G were found to have higher mutant allele frequencies in case individuals indicating that had a larger cohort been used, significance may have been observed.Conclusions: This study was able to generate genotypic information for a South African cohort for both reported and understudied mutations. Furthermore, the identification of higher mutant allele frequencies for C16223T, A10398G and A8536G highlights the importance of considering these mutations in future studies using a larger cohort.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"288"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11889028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FTO-mediated Nrf2 demethylation alleviates high glucose-induced oxidative stress and apoptosis in retinal pigment epithelial cells.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-07 DOI: 10.1007/s11033-025-10400-x

Quan Cheng, Liqiong Zhou, Xinyu Fan, Minjun Ma, Chunhui Zhang, Xu Zha, Yuanping Zhang

{"title":"FTO-mediated Nrf2 demethylation alleviates high glucose-induced oxidative stress and apoptosis in retinal pigment epithelial cells.","authors":"Quan Cheng, Liqiong Zhou, Xinyu Fan, Minjun Ma, Chunhui Zhang, Xu Zha, Yuanping Zhang","doi":"10.1007/s11033-025-10400-x","DOIUrl":"https://doi.org/10.1007/s11033-025-10400-x","url":null,"abstract":"Background: N6-methyladenosine (m6A) modification contributes to the development of diabetic retinopathy (DR). This study aimed to reveal the role and downstream regulatory signaling of an m6A demethylase fat mass and obesity-associated gene (FTO) in high glucose-induced damage of retinal pigment epithelial cells.Methods and results: By stimulating ARPE-19 cells with different concentrations of glucose (0 mM-50 mM), we observed that FTO expression was significantly downregulated, while m6A modification level was upregulated in a glucose concentration-dependent manner in ARPE-19 cells. Then, ARPE-19 cells were transfected with FTO knockdown or overexpression vector, and administrated with high glucose (25mM) to perform functional verification experiments. FTO overexpression recovered cell viability, inhibited cell apoptosis, elevated GSH/GSSG ratio, but reduced MDA and ROS levels in high glucose-induced cells, while FTO knockdown further exacerbated high glucose-triggered oxidative stress and apoptotic cell death. Additionally, FTO overexpression upregulated the expression of NF-E2-related factor 2 (Nrf2) and activated the antioxidant heme oxygenase 1 (HO-1) signal through m6A demethylation on Nrf2 in high glucose-treated ARPE-19 cells. Finally, we proved that knockdown of Nrf2 or HO-1 reversed the protective effects of FTO overexpression on high glucose-treated ARPE-19 cells.Conclusion: Altogether, the study demonstrated that FTO ameliorates high glucose-triggered oxidative stress and cell apoptosis through activating the Nrf2/HO-1 signaling pathway in an m6A-dependent manner.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"289"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of 5-azacytidine and N6-methyladenosine combination on apoptosis and stemness in human breast cancer stem cells.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-07 DOI: 10.1007/s11033-025-10398-2

Gunel Mukhtarova, Mesude Angin, Ayse Caner, Cumhur Gunduz

{"title":"Effects of 5-azacytidine and N6-methyladenosine combination on apoptosis and stemness in human breast cancer stem cells.","authors":"Gunel Mukhtarova, Mesude Angin, Ayse Caner, Cumhur Gunduz","doi":"10.1007/s11033-025-10398-2","DOIUrl":"https://doi.org/10.1007/s11033-025-10398-2","url":null,"abstract":"Background: This study investigates the combined effects of the epigenetic anticancer drug 5-azacytidine (5-Aza) and N6-methyladenosine (m6A) on breast cancer stem cells (CSCs) and normal breast epithelial cells. CSCs are characterized by their ability to self-renew, their resistance to conventional therapies, and their role in metastasis, presenting a significant challenge in breast cancer treatment.Methods and results: The study utilized flow cytometry to isolate CD44 + /CD24low CSCs from MCF-7 breast cancer cells and evaluated these cells through spheroid formation assays. The results demonstrated that both 5-Aza and m6A, both individually and in combination, exert cytotoxic effects on CSCs, induce apoptosis, and reduce their migratory capacity. Importantly, these treatments did not produce similar effects on normal breast epithelial cells (MCF-10A), indicating selective action on CSCs. Gene expression analysis revealed that treatment with 5-Aza, m6A, and their combination altered the expression of key stem cell-related genes, including OCT4, NANOG, SOX2, and c-MYC, which are associated with CSC self-renewal and malignancy.Conclusions: These findings suggest that epigenetic modulation through 5-Aza and m6A could effectively target CSCs, disrupting their ability to drive tumor progression and metastasis, particularly in aggressive breast cancer subtypes. This study highlights the potential of 5-Aza and m6A as a combinatorial therapeutic approach, offering a promising avenue for improving treatment outcomes in breast cancer patients, especially those with therapy-resistant disease. Further clinical investigation is needed to validate these findings and explore their therapeutic implications.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"292"},"PeriodicalIF":2.6,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143586329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancement insights in cancer vaccines: mechanisms, types, and clinical applications.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-07 DOI: 10.1007/s11033-025-10370-0

Gellan Alaa Mohamed Kamel, Rasha A Attia, Hifaa G Al-Noman, Lamiaa A Salama

引用次数: 0

Discovering the pathogenesis of a VUS variant in CDH23 associated with sensorineural hearing loss in an Iranian family.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-06 DOI: 10.1007/s11033-025-10401-w

Maryam Naghinejad, Sima Mansoori Derakhshan, Sepideh Parvizpour, Akbar Amirfiroozy

{"title":"Discovering the pathogenesis of a VUS variant in CDH23 associated with sensorineural hearing loss in an Iranian family.","authors":"Maryam Naghinejad, Sima Mansoori Derakhshan, Sepideh Parvizpour, Akbar Amirfiroozy","doi":"10.1007/s11033-025-10401-w","DOIUrl":"https://doi.org/10.1007/s11033-025-10401-w","url":null,"abstract":"Background: Hearing loss (HL) is a highly heterogeneous condition with concerning statistics, particularly given the significant occurrence of consanguineous marriages in Iran. Despite the presence of variants of uncertain significance (VUS), high-yield prenatal screening remains unattainable. This article aims to discuss a VUS mutation as a potential cause of HL in a sibling, with the goal of reclassifying this variant.Methods: This research examined a sibling with congenital HL. Their parents were first cousins, and no non-genetic factors for the disease were identified. Whole exome sequencing (WES) was conducted to ascertain the genetic etiology of the disease, subsequently validated by Sanger sequencing. The stability and interactions of the mutated protein were then evaluated.Results: The homozygous variant CDH23 (NM_022124.6) c.5149T > C; p.C1717R has been suggested as the probable causative mutation. This variant was identified as a homozygous mutant in both patients and a heterozygous variant in healthy individuals. The mutation enhances the stability of this protein, whereas the interaction with the protein PCDH15, which plays a role in hemophilic stereocilia attachment, is entirely abolished in the areas surrounding the mutation. This protein interaction site is completely altered post-mutation, significantly reducing complex stability.Conclusion: A homozygous variant in the CDH23 gene was identified in two patients from the same pedigree as a consequence of this investigation. Consequently, in light of the findings of this investigation, it is advised that additional in silico studies, including molecular dynamics simulations and in vitro studies, be conducted to assist in the reclassification of this variant, thereby enabling more precise genetic counseling.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"284"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

DNA barcoding of geographical indication tagged Byadagi chilli and its cultivars using ITS2, matK and rbcL coding sequences.

IF 2.6 4区生物学

Molecular Biology Reports Pub Date : 2025-03-06 DOI: 10.1007/s11033-025-10379-5

B N Meghana, S V Reshma

{"title":"DNA barcoding of geographical indication tagged Byadagi chilli and its cultivars using ITS2, matK and rbcL coding sequences.","authors":"B N Meghana, S V Reshma","doi":"10.1007/s11033-025-10379-5","DOIUrl":"https://doi.org/10.1007/s11033-025-10379-5","url":null,"abstract":"Background: Byadagi chilli, a Geographical Indication (GI)-tagged chilli variety known for its special aroma and bright red colour, was accorded the GI tag in February 2011 with the GI number 129. The two traditional varieties of Byadagi chilli, namely: Dabbi and Kaddi, are the GI-tagged varieties. In this study, GI-tagged Byadagi Dabbi, Byadagi Kaddi and other cultivars of Byadagi chilli, such as Byadagi Lali (BL), Byadagi HPH 2043 (B2), Byadagi BSS 355 (B3) and another popular GI-tagged chilli variety, Guntur Sannam, were analysed to assess their inter-relationships. Due to the high market value and demand, it is important to identify and differentiate the original variety of Byadagi chilli and its associated cultivars from the other chilli cultivars, which are sold under the name of Byadagi chilli. In this study, molecular assessment by DNA barcoding was performed to establish the identity of authentic Byadagi chilli varieties.Methods and results: Five samples of Byadagi chilli and another GI-tagged chilli variety, Guntur Sannam, were analysed using three DNA barcodes: ITS2, matK and rbcL. The PCR products were sequenced, nucleotide BLAST was performed and ITS2 showed 97.7% identity, matK 99.1%, and rbcL 99.19% with Capsicum annuum at the genus and species levels. Phylogenetic analysis of the DNA sequences of all the six chilli samples was performed using ClustalW multiple sequence alignment in MEGA11. The genetic distance between the six samples was calculated using the maximum likelihood approach.Conclusions: This study distinctly demonstrates that the chloroplast DNA barcodes matK and rbcL, along with the nuclear DNA barcode, ITS2, can be used for accurate identification of Byadagi chilli cultivars. This study offers significant molecular identification and establishes a robust barcoding foundation for Byadagi chilli. The phylogenetic trees generated from the barcode sequences clearly indicated the relationships among the selected cultivars.","PeriodicalId":18755,"journal":{"name":"Molecular Biology Reports","volume":"52 1","pages":"286"},"PeriodicalIF":2.6,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0