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Taxonomic Specificity of the Sensitivity to the Wickerhamomyces bovis Fungistatic Mycocin. 牛柳杉霉菌抑菌素敏感性的分类特异性。
Mikrobiologiia Pub Date : 2016-07-01
W I Golubev
{"title":"Taxonomic Specificity of the Sensitivity to the Wickerhamomyces bovis Fungistatic Mycocin.","authors":"W I Golubev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Wickerhamomyces bovis type strain was found to secret a mycocin with a fungistatic effect at pH from 3.5 to 6.0. The peak of its activity occurred at pH 5.0 in the presence of 3% NaCl. Yeast species sensitive to this mycocin belonged to phylogenetically related genera Ambrosiozyma, Nakazawaea, Ogataea, and Pet- erozyma within the family Wickerhamomycetaceae.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 4","pages":"415-420"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35309967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Broiler Cecal Microbiocenoses Depending on Mixed Fodder. 混合饲料对肉鸡盲肠微生物群落的影响。
Mikrobiologiia Pub Date : 2016-07-01
V I Fisinin, L A Il'ina, E A Iyldyrym, I N Nikonov, V A Filippova, G Yu Laptev, N I Novikova, A A Grozina, T N Lenkova, V A Manukya, I A Egorov
{"title":"Broiler Cecal Microbiocenoses Depending on Mixed Fodder.","authors":"V I Fisinin,&nbsp;L A Il'ina,&nbsp;E A Iyldyrym,&nbsp;I N Nikonov,&nbsp;V A Filippova,&nbsp;G Yu Laptev,&nbsp;N I Novikova,&nbsp;A A Grozina,&nbsp;T N Lenkova,&nbsp;V A Manukya,&nbsp;I A Egorov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Molecular genetic techniques (NGS sequencing and quantitative PCR) were used to determine the composition of the cecal bacterial community of broiler chickens fed with different mixed fodder. The Cecal microbiome exhibited taxonomic diversity, with both typical inhabitants of avian intestine belonging to the families Clostridiaceae, Eubacteriaceae, and Lactobacillaceae and to the phylum Bacteroidetes, and new un- identified taxa, as well as bacteria of the families Lachnospiraceae and Ruminococcaceae, which were previ- ously considered restricted to the rumen microflora. Contrary to traditional concepts, enterococci and bi- fidobacteria were among the minor components of the community, lactate-fermenting species were absent, and typical avian pathogens of the genus Staphylococcus were detected but seldom. Members of the family Suterellaceae and the genus Gallibacterium, which are responsible for avian respiratory infections, were also detected. Significant fluctuations of abundance and composition of microbial groups within the cecal com- munity and of the parameters of broiler productivity were found to occur depending on the feed allowance. Cellulose content in the feed had the most pronounced effect on the composition aid structure of bacterial communities. Decreased cellulose content resulted in a decrease of bacterial abundance by-aii order of mag- nitude and in increased ratios of members of the phylum Bacteroidetes and the family Clostridiaceae, which possess the enzymes degrading starch polysadcharides. Abundance of the normal inhabitants of avian intes- tine belonging to the genus Ldctobacillus and the order Bacillales decreased, while the share of Escherichia and members of the family Sutterellaceae increased, including some species capable of causing dysbiotic changes in avian intestine. No significant change in abundance of cellulolytics of the families Ruminococca- ceae, Lachnospiraceae, and Eubacteriaceae was observed.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 4","pages":"472-480"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35457341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Modern Methods for Isolation, Purification, and Cultivation of Soil Cyanobacteria. 分离、纯化和培养土壤蓝藻的现代方法。
Mikrobiologiia Pub Date : 2016-07-01
A D Temraleeva, S A Dronova, S V Moskalenko, S V Didovich
{"title":"Modern Methods for Isolation, Purification, and Cultivation of Soil Cyanobacteria.","authors":"A D Temraleeva,&nbsp;S A Dronova,&nbsp;S V Moskalenko,&nbsp;S V Didovich","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Up-to-date methods for isolation of cyanobacteria from soil samples, removal of accompanying microflora, obtaining axenic strains, and -conditions and media for subsequnt cultivation are reviewed. Char acterization of soil as a specific habitat for cyanobacteria is provided. Comparative analysis of pH and ele- mental composition of the liquid phase of most soil types with the media for cultivating cyanobacteria is car- ried out. The functional role of the major components required for the cultivation of cyanobacteria is de- scribed. The problems associated with isolation, purification, and cultivation of soil cyanobacteria, as well as the relevant solutions, are discussed.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 4","pages":"369-380"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35309963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trophic Interactions of Proteolytic Bacteria Proteinivorax tanatarense in an Alkalinphilic Microbial Community. 蛋白水解菌在嗜碱微生物群落中的营养相互作用。
Mikrobiologiia Pub Date : 2016-07-01
Yu V Boltyanskaya, V V Kevbrin
{"title":"Trophic Interactions of Proteolytic Bacteria Proteinivorax tanatarense in an Alkalinphilic Microbial Community.","authors":"Yu V Boltyanskaya,&nbsp;V V Kevbrin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Lythic action of an anaerobic proteolytic bacterium Proteinivorax tanatarense on organisms with different cell wall types was studied. In the absence of photosynthetic oxygen release, this proteolytic was able to grow on intact biomass of cyanobacteria belonging to various systematic groups. Itis probably their usual saprotrophic-satellite responsible for the regulation of abundance of primary producers during the dark phase. Growth also occurred on the biomass of a nonphototrophic gram-negative microorganism-Halomonas campisalis, a common component of alkaliphilic. microbial communities: Comparative analysis of the interaction of the proteolytic with.H. campisalis cells at different physiological states revealed the lytic action to be re- stricted to dead and/or weakened cells, rather than the actively dividing ones. Strict specificity of the action of the proteolytic bacterium on gram-negative microorganisms with no effect on gram-positive ones was shown.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 4","pages":"458-465"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35457339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[In process]. (过程中)。
Mikrobiologiia Pub Date : 2016-07-01
{"title":"[In process].","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 4","pages":"490-492"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35457344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Changes in Cell Surface Properties and Biofilm Formation Efficiency in Azospirillum brasilense Sp245 Mutants in the Putative Genes of Lipid Metabolism mmsB1 and fabG1]. [脂质代谢基因mmsB1和fabG1突变体对巴西氮螺旋菌Sp245细胞表面特性和生物膜形成效率的影响]。
Mikrobiologiia Pub Date : 2016-03-01
E Shumilova, A V Shelud'ko, Yu A Filip'echeva, S S Evstigneeva, E G Ponomareva, L P Petrova, E I Katsy
{"title":"[Changes in Cell Surface Properties and Biofilm Formation Efficiency in Azospirillum brasilense Sp245 Mutants in the Putative Genes of Lipid Metabolism mmsB1 and fabG1].","authors":"E Shumilova,&nbsp;A V Shelud'ko,&nbsp;Yu A Filip'echeva,&nbsp;S S Evstigneeva,&nbsp;E G Ponomareva,&nbsp;L P Petrova,&nbsp;E I Katsy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The previously obtained insertion mutants ofAzospirillum brasilense Sp245 in the genes mmsBl and fabG1 (strains SK039 and Sp245.1610, respectively) were characterized by impaired flagellation and motility. The putative products of expression of these genes are 3-hydroxyisobutyrate dehydrogenase and 3-oxoacyl-[acyl-carrier protein] reductase, respectively. In the present work, A. brasilense- Sp245 strains SK039 and Sp245.1610 were found to have differences in the content of 3-hydroxyhexadecanoic, hexadecanoic, 3-hydroxytetradecanoic, hexadecenoic, octadecenoic, and nonadecanoic acids in their lipopolysaccharide prepa- rations, as well as in cell hydrophobicity and hemagglutination activity and dynamics of cell aggregation, in biomass amount, and in the relative content of lipopolysaccharide antigens in mature biofilms formed on hydrophilic or hydrophobic surfaces.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 2","pages":"162-70"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34609449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Amylase- and Protease-Producing Staphylococcus Bacteria in a Geothermal Spring of Makhachkala Deposit (Dagestan)]. [Makhachkala矿床(达吉斯坦)地热泉中产淀粉酶和蛋白酶的葡萄球菌]。
Mikrobiologiia Pub Date : 2016-03-01
E A Khalilova, T P Tourova, S Ts Kotenko, E A Islammagomedova, D A Aliverdieva
{"title":"[Amylase- and Protease-Producing Staphylococcus Bacteria in a Geothermal Spring of Makhachkala Deposit (Dagestan)].","authors":"E A Khalilova,&nbsp;T P Tourova,&nbsp;S Ts Kotenko,&nbsp;E A Islammagomedova,&nbsp;D A Aliverdieva","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 2","pages":"219-22"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34621513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Evolution of Root Nodule Bacteria: Reconstruction of the Speciation Processes Resulting from Genomic Rearrangements in a Symbiotic System]. 根瘤细菌的进化:共生系统中由基因组重排引起的物种形成过程的重建。
Mikrobiologiia Pub Date : 2016-03-01
N A Provorov, E E Andronov
{"title":"[Evolution of Root Nodule Bacteria: Reconstruction of the Speciation Processes Resulting from Genomic Rearrangements in a Symbiotic System].","authors":"N A Provorov,&nbsp;E E Andronov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The processes of speciation and macroevolution of root nodule bacteria (rhizobia), based on deep rearrangements of their genomes and occurring in the N₂-fixing symbiotic system, are reconstructed. At the first stage of rhizobial evolution, transformation of free-living diazotrophs (related to Rhodopseudomonas) to symbiotic N₂-fixers (Bradyrhizobium) occurred due to the acquisition of the fix gene system, which is responsible for providing nitrogenase with electrons and reducing equivalents, as well as for oxygen-dependent regulation of nitrogenase synthesis in planta, and then of the nod genes responsible for the synthesis of the lipo- chito-oligosaccharide Nod factors, which induce root nodule development. The subsequent rearrangements of bacterial genomes included: (1) increased volume of hereditary information supported by species, genera (pan-genome), and individual strains; (2) transition from the unitary genome to a multicomponent one; and (3) enhanced levels of bacterial genetic plasticity and horizontal gene transfer, resulting in formation of new genera, of which Mesorhizobium, Rhizobium, and Sinorhizobium are the largest, and of over 100 species. Rhizobial evolution caused by development and diversification of the Nod factor synthesizing systems may result in both increased host specificity range (transition of Bradyrhizobium from autotrophic to symbiotrophic carbon metabolism in interaction with a broad spectrum of legumes) and to its contraction (transition of Rhizobium and Sinorhizobium to \"altruistic\" interaction with legumes of the galegoid clade). Reconstruction of the evolutionary pathway from symbiotic N₂-fixers to their free-living ancestors makes it possible to initiate the studies based on up-to-date genome screening technologies and aimed at the issues of genetic integration of organisms into supracpecies complexes, ratios of the macro- and microevolutionary mechanisms, and developmetn of cooperative adaptations based on altruistic relationship between the symbiotic partners.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 2","pages":"115-25"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34609446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Role of Anammox Bacteria in Removal of Nitrogen Compounds from Wastewater]. 厌氧氨氧化菌在去除废水中氮化合物中的作用
Mikrobiologiia Pub Date : 2016-03-01
A Yu Kallistovaa, A G Dorofeev, Yu A Nikolaev, M N Kozlov, M V Kevbrina, N V Pimenov
{"title":"[Role of Anammox Bacteria in Removal of Nitrogen Compounds from Wastewater].","authors":"A Yu Kallistovaa,&nbsp;A G Dorofeev,&nbsp;Yu A Nikolaev,&nbsp;M N Kozlov,&nbsp;M V Kevbrina,&nbsp;N V Pimenov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The review deals with the unique microbial group responsible for anaerobic ammonium oxidation with nitrite (anammox), and with the role of this process in development of the biotechnology for removal of nitrogen compounds from wastewater. The history of the study of this process is briefly related. Up-to date knowledge on the intracellular organization, energy metabolism, growth stoichiometry, and physiology of anammox bacteria is described, and the main methods for cultivation of these microorganisms are characterized. Special attention is paid to the problems associated with practical application of anammox bacteria, which result from their extremely slow growth, the absence of pure cultures, and the interaction with other microbial groups.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 2","pages":"126-44"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34609448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Metagenomic Analysis of Microbial Communities of the Sediments of the Kara Sea Shelf and the Yenisei Bay]. [卡拉海陆架和叶尼塞湾沉积物微生物群落的宏基因组分析]。
Mikrobiologiia Pub Date : 2016-03-01
E V Mamaeva, Yu P Galach'yants, K V Khabudaev, D P Petrova, T V Pogodaeva, T B Khodzher, T I Zemskaya
{"title":"[Metagenomic Analysis of Microbial Communities of the Sediments of the Kara Sea Shelf and the Yenisei Bay].","authors":"E V Mamaeva,&nbsp;Yu P Galach'yants,&nbsp;K V Khabudaev,&nbsp;D P Petrova,&nbsp;T V Pogodaeva,&nbsp;T B Khodzher,&nbsp;T I Zemskaya","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Microbial diversity in the sediments of the Kara Sea shelf and the southern Yenisei Bay, differing in pore water mineralization, was studied using massive parallel pyrosequencing according to the 454 (Roche) technology. Members of the same phyla (Cyanobacteria, Verrucomicrobia, Actinobacteria, Proteobacteria, and Bacteroidetes) predominated in bacterial communities of the sediments, while their ratio and taxonomic composition varied within the phyla and depended on pore water mineralization. Increasing salinity gradient was found to coincide with increased share of the γ-Proteobacteria and decreased abundance of α- and β-Proteo- bacteria, as well as of the phyla Verrucomicrobia, Chloroflexi, Chlorobi, and Acidobacteria. Archaeal diversity was lower, with Thaumarchaeota predominant in the sediments with high and low mineralization, while Crenarchaeota predominated in moderately mineralized sediments. Microbial communities of the Kara Sea shelf and Yenisei Gulf sediments were found to contain the organisms capable of utilization of a broad spectrum of carbon sources, including gaseous and petroleum hydrocarbons.</p>","PeriodicalId":18732,"journal":{"name":"Mikrobiologiia","volume":"85 2","pages":"187-98"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34621504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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