Molecular and biochemical parasitology最新文献_第6页

Characterisation of TcFUT1, a mitochondrial fucosyltransferase from Trypanosoma cruzi 克鲁兹锥虫线粒体岩藻糖基转移酶TcFUT1的特性研究

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-08-29 DOI: 10.1016/j.molbiopara.2023.111590

Jose Carlos Paredes Franco, Maria Lucia Sampaio Guther, Marta L. Lima, Michael A.J. Ferguson

{"title":"Characterisation of TcFUT1, a mitochondrial fucosyltransferase from Trypanosoma cruzi","authors":"Jose Carlos Paredes Franco, Maria Lucia Sampaio Guther, Marta L. Lima, Michael A.J. Ferguson","doi":"10.1016/j.molbiopara.2023.111590","DOIUrl":"10.1016/j.molbiopara.2023.111590","url":null,"abstract":"<div>Previous work has shown that the TbFUT1 and LmjFUT1 genes encode essential fucosyltransferases located inside the single mitochondria of the protozoan parasites Trypanosoma brucei and Leishmania major, respectively. However, nothing was known about the orthologous gene TcFUT1 or its gene product in Trypanosoma cruzi, aetiological agent of Chagas disease. In this study, we describe the overexpression of TcFUT1 with a C-terminal 6xMyc epitope tag in T. cruzi epimastigote cells. Overexpressed and immunoprecipitated TcFUT1–6xMyc was used to demonstrate enzymatic activity and to explore substrate specificity. This defined TcFUT1 as a GDP-Fuc : βGal α1–2 fucosyltransferase with a strict requirement for acceptor glycans with non-reducing terminal Galβ1–3GlcNAc structures. This differs from the specificity of the T. brucei orthologue TbFUT1, which can also tolerate non-reducing terminal Galβ1–4GlcNAc and Galβ1–4Glc acceptor sites. Immunofluorescence microscopy using α-Myc tag antibodies also showed a mitochondrial location for TcFUT1 in T. cruzi epimastigote cells. Collectively, these results are like those described for TbFUT1 and LmjFUT1 from T. brucei and L. major, suggesting that FUT1 gene products have conserved function for across the trypanosomatids and may share therapeutic target potential.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"256 ","pages":"Article 111590"},"PeriodicalIF":1.5,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10200999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the major surface glycoconjugates of Trypanosoma theileri 泰勒锥虫主要表面糖缀合物的特性

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-08-29 DOI: 10.1016/j.molbiopara.2023.111591

Rupa Nagar , Isobel Hambleton , Michele Tinti , Mark Carrington , Michael A.J. Ferguson

{"title":"Characterization of the major surface glycoconjugates of Trypanosoma theileri","authors":"Rupa Nagar , Isobel Hambleton , Michele Tinti , Mark Carrington , Michael A.J. Ferguson","doi":"10.1016/j.molbiopara.2023.111591","DOIUrl":"10.1016/j.molbiopara.2023.111591","url":null,"abstract":"<div>Trypanosoma theileri maintains a long-term extracellular infection with a low parasitaemia in bovids. The surface of this parasite is predicted to be decorated with several surface molecules including membrane surface proteases (MSPs), trans-sialidases and T. theileri putative surface proteins (TTPSPs). However, there are no experimental data to verify this hypothesis. Here, we have purified and partially characterized the surface glycoconjugates of T. theileri using biochemical and mass spectrometry-based approaches. The glycoconjugates fall into two classes: glycoproteins and glycolipids. Proteomic analysis of the glycoprotein fraction demonstrated the presence of MSPs and abundant mucin-like TTPSPs, with most predicted to be GPI-anchored. Mass spectrometric characterization of the glycolipid fraction showed that these are mannose- and galactose-containing glycoinositolphospholipids (GIPLs) that are larger and more diverse than those of its phylogenetic relative T. cruzi, containing up to 10 hexose residues and carrying either alkylacyl-phosphatidylinositol or inositol-phospho-ceramide (IPC) lipid components.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"256 ","pages":"Article 111591"},"PeriodicalIF":1.5,"publicationDate":"2023-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10140824","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does hydrogen peroxide contribute to the immunity against Malaria induced by whole attenuated plasmodial sporozoites? 过氧化氢是否有助于对整个减毒疟原虫子实体诱导的疟疾免疫？

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-08-19 DOI: 10.1016/j.molbiopara.2023.111589

Bruno Douradinha

引用次数: 0

Novel anti-Acanthamoebic properties of raloxifene sulfonate/sulfamate derivatives 雷洛昔芬磺酸酯/氨基磺酸盐衍生物的抗棘阿米巴新特性

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-08-09 DOI: 10.1016/j.molbiopara.2023.111582

Ruqaiyyah Siddiqui , Mohammed I. El-Gamal , Sreedevi Sajeev , Seyed-Omar Zaraei , Naveed Ahmed Khan

{"title":"Novel anti-Acanthamoebic properties of raloxifene sulfonate/sulfamate derivatives","authors":"Ruqaiyyah Siddiqui , Mohammed I. El-Gamal , Sreedevi Sajeev , Seyed-Omar Zaraei , Naveed Ahmed Khan","doi":"10.1016/j.molbiopara.2023.111582","DOIUrl":"10.1016/j.molbiopara.2023.111582","url":null,"abstract":"<div>Acanthamoeba are known to cause a vision threatening eye infection typically due to contact lens wear, and an infection of the central nervous system. The ability of these amoebae to switch phenotypes, from an active trophozoite to a resistant cyst form is not well understood; the cyst stage is often resistant to chemotherapy, which is of concern given the rise of contact lens use and the ineffective disinfectants available, versus the cyst stage. Herein, for the first time, a range of raloxifene sulfonate/sulfamate derivatives which target nucleotide pyrophosphatase/phosphodiesterase enzymes, were assessed using amoebicidal and excystation tests versus the trophozoite and cyst stage of Acanthamoeba. Moreover, the potential for cytopathogenicity inhibition in amoebae was assessed. Each of the derivatives showed considerable anti-amoebic activity as well as the ability to suppress phenotypic switching (except for compound 1a). Selected raloxifene derivatives reduced Acanthamoeba-mediated host cell damage using lactate dehydrogenase assay. These findings suggest that pyrophosphatase/phosphodiesterase enzymes may be valuable targets against Acanthamoeba infections.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"256 ","pages":"Article 111582"},"PeriodicalIF":1.5,"publicationDate":"2023-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10559305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Omega-class glutathione transferases in helminths of the Taeniidae family 带绦虫科蠕虫中Omega类谷胱甘肽转移酶的鉴定

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111559

Sebastián Miles, Gustavo Mourglia-Ettlin, Verónica Fernández

{"title":"Identification of Omega-class glutathione transferases in helminths of the Taeniidae family","authors":"Sebastián Miles, Gustavo Mourglia-Ettlin, Verónica Fernández","doi":"10.1016/j.molbiopara.2023.111559","DOIUrl":"10.1016/j.molbiopara.2023.111559","url":null,"abstract":"<div>Glutathione transferase enzymes (GSTs) are believed to be a major detoxification system in helminth parasites and have been associated with immunomodulation of the host response. Echinococcus granulosus sensu lato (s.l.) is a cestode parasite known to express at least five different GSTs, but no Omega-class enzymes have been reported in this parasite or in any other cestode. Herein we report the identification of a new member of the GST superfamily in E. granulosus s.l., which is phylogenetically related to the Omega-class: EgrGSTO. Through mass spectrometry, we showed that the 237 amino acids protein EgrGSTO is expressed by the parasite. Moreover, we identified homologues of EgrGSTO in other eight members of the Taeniidae family, including E. canadensis, E. multilocularis, E. oligarthrus, Hydatigera taeniaeformis, Taenia asiatica, T. multiceps, T. saginata and T. solium. A manual sequence inspection and rational modification yielded eight Taeniidae’s GSTO sequences, each one encoding for a 237 aa polypeptide showing 80.2% overall identity. To the best of our knowledge, this is the first description of genes encoding for Omega-class GSTs in worms belonging to the Taeniidae family -that at least in E. granulosus s.l. is expressed as a protein- suggesting the gene encodes for a functional protein.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"254 ","pages":"Article 111559"},"PeriodicalIF":1.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9436359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics of Th9 cells in Schistosoma japonicum-infected C57BL/6 mouse mesenteric lymph node 日本血吸虫感染C57BL/6小鼠肠系膜淋巴结的Th9细胞特性

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111561

Huaina Qiu , Ruohan Wang , Junmin Xing , Lu Li , Zhiyan Gao , Jiajie Li , Chao Fang , Feihu Shi , Feng Mo , Lin Liu , Yi Zhao , Hongyan Xie , Shan Zhao , Jun Huang

{"title":"Characteristics of Th9 cells in Schistosoma japonicum-infected C57BL/6 mouse mesenteric lymph node","authors":"Huaina Qiu , Ruohan Wang , Junmin Xing , Lu Li , Zhiyan Gao , Jiajie Li , Chao Fang , Feihu Shi , Feng Mo , Lin Liu , Yi Zhao , Hongyan Xie , Shan Zhao , Jun Huang","doi":"10.1016/j.molbiopara.2023.111561","DOIUrl":"10.1016/j.molbiopara.2023.111561","url":null,"abstract":"<div>Interleukin 9 (IL-9) is an effective cytokine secreted by newly defined Th9 cells, which is involved in allergic and infectious diseases. In this study, lymphocytes were isolated from mesenteric lymph node (MLN), spleen, liver, lung, and Peyer’s patches (PP) of C57BL/6 mice 5–6 weeks after S. japonicum infection, intracellular cytokine staining was done to detect the percentage of IL-9-producing CD4+ T cells. The qPCR and ELISA were used to verify the content of IL-9 in MLN. The population of IL-9-producing lymphocyte subset was identified by FACS. In addition, the dynamic changes and cytokine profiles of Th9 cells in the MLN of infected mice were detected by FACS. ELISA was used to detect IL-9 induced by soluble egg antigen (SEA) from isolated lymphocytes in mouse MLN. The results showed that the percentage of IL-9-secreting Th9 cells in the MLN of the infected mouse was higher than that in the spleen, liver, lung, or PP. Though CD8+ Tc cells, NKT cells, and γδT cells could secrete IL-9, CD4+ Th cells were the main source of IL-9 in S. japonicum-infected C57BL/6 mice (P < 0.05). The percentage of Th9 cells in MLN of infected mouse increased from week 3–4, and reached a peak at week 5–6, then began to decrease from week 7–8 (P < 0.05). Moreover, Th9 cells could also secrete a small amount of IL-4, IFN-γ, IL-5, and IL-10. Our results suggested a higher percentage of Th9 cells was induced in the MLN of S. japonicum-infected mice, which might play an important role in the early stage of S. japonicum-induced disease.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"254 ","pages":"Article 111561"},"PeriodicalIF":1.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9438648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples 从滤纸血样中提取恶性疟原虫DNA的简易碱裂解法

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111557

Zheng Xiang , Danlin Li , Siqi Wang , Ting Shen , Wen He , Mier Li , Weilin Zeng , Xi Chen , Yanrui Wu , Liwang Cui , Zhaoqing Yang

{"title":"A simple alkali lysis method for Plasmodium falciparum DNA extraction from filter paper blood samples","authors":"Zheng Xiang , Danlin Li , Siqi Wang , Ting Shen , Wen He , Mier Li , Weilin Zeng , Xi Chen , Yanrui Wu , Liwang Cui , Zhaoqing Yang","doi":"10.1016/j.molbiopara.2023.111557","DOIUrl":"10.1016/j.molbiopara.2023.111557","url":null,"abstract":"<div>A fast, simple, easy, efficient, and inexpensive method for DNA extraction from malaria parasites collected on filter paper would be very useful for molecular surveillance. The quality and quantity of DNA are critical to molecular diagnosis and analysis. Here, we developed a simple alkali lysis method for DNA extraction from blood samples on filter paper. The results showed that 10–50 mM NaOH and deionized water all effectively isolated parasite DNA at higher parasitemia, as witnessed by successful PCR amplification, while at a parasitemia of 0.01%, the 10 mM NaOH lysis condition generated the best results. Furthermore, DNA extracted by this method was successfully used to amplify a fragment of > 2000 bp. This method successfully extracted DNA from 1 µl of blood at a parasitemia as low as 0.0001% (equivalent to 5 parasites /µl). The DNA isolated by the 10 mM NaOH lysis method was stable to yield PCR products after storage at 4 °C or − 20 °C for 12 months. These results indicate that this alkali lysis method is simple, effective, sensitive, and inexpensive for isolating stable Plasmodium DNA from dried blood spots on filter paper.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"254 ","pages":"Article 111557"},"PeriodicalIF":1.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10214771/pdf/nihms-1898997.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9913867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression 绵羊外周血单个核细胞对细粒棘球蚴microRNA-71过度表达的反应分析

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111556

Yating Li , Lujun Yan , Duojie Ci , Rui Li , Wanjing Li , Tianqi Xia , Hengzhi Shi , Mazhar Ayaz , Yadong Zheng , Pu Wang

{"title":"Analysis of sheep peripheral blood mononuclear cells in response to Echinococcus granulosus microRNA-71 overexpression","authors":"Yating Li , Lujun Yan , Duojie Ci , Rui Li , Wanjing Li , Tianqi Xia , Hengzhi Shi , Mazhar Ayaz , Yadong Zheng , Pu Wang","doi":"10.1016/j.molbiopara.2023.111556","DOIUrl":"10.1016/j.molbiopara.2023.111556","url":null,"abstract":"<div>Cyst echinococcosis, caused by Echinococcus granulosus, remains a zoonotic disease posing a great threat to public health and meat production industry. Sheep infected with E. granulosus show relatively high abundance of egr-miR-71 in the sera, but its role is unknown. Using bioinformatics and cell migration and Transwell assays, we comparatively analyzed the proteomes and cell invasion of sheep PBMCs in response to egr-miR-71 overexpression. The results showed that the egr-miR-71 induced a total of 157 proteins being differentially expressed and mainly involved in immune responses. In sheep PBMCs, egr-miRNA-71 overexpression induced significant downregulation of macrophage migration inhibitory factor (MIF) and accordingly promoted cell migration and invasion compared with the control. The results will provide a clue for further investigation of a role of circulating egr-miR-71 in immune responses during E. granulosus infection.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"254 ","pages":"Article 111556"},"PeriodicalIF":1.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9435289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro and in vivo evaluation of efficacy of berberine chloride: Phyto-alternative approach against Trypanosoma evansi infection 盐酸黄连素对伊氏锥虫感染的体内外疗效评价：植物替代方法

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111562

Snehil Gupta , Sukhdeep Vohra , Khushboo Sethi , Ruma Rani , Surbhi Gupta , Sanjay Kumar , Rajender Kumar

{"title":"In vitro and in vivo evaluation of efficacy of berberine chloride: Phyto-alternative approach against Trypanosoma evansi infection","authors":"Snehil Gupta , Sukhdeep Vohra , Khushboo Sethi , Ruma Rani , Surbhi Gupta , Sanjay Kumar , Rajender Kumar","doi":"10.1016/j.molbiopara.2023.111562","DOIUrl":"10.1016/j.molbiopara.2023.111562","url":null,"abstract":"<div>Current chemotherapy against the Surra organism, Trypanosoma evansi has several limitations in terms of efficacy, toxicity, availability and emerging resistance. These reasons make the search of new chemo-preventive and chemo-therapeutic agent with high potency and low toxicity. Alkaloid phyto-molecules, berberine has shown promising anti-kinetoplastid activity against T. cruzi, T. congolense, T. brucei, Leishmania donovani and L. tropica. However, till date, there is no investigation of therapeutic efficacy of berberine chloride (BC) against T. evansi. The IC50 value of BC for growth inhibition of T. evansi at 24 h of culture was calculated as 12.15 µM. The specific selectivity index (SSI) of BC was calculated as 19.01 and 10.43 against Vero cell line and Equine PBMC’s, respectively. Thirteen drug target genes affecting various metabolic pathways were studied to investigate the mode of trypanocidal action of BC. In transcript analysis, the mRNA expression of arginine kinase 1 remained refractory to exposure with BC, which provides metabolic plasticity in adverse environmental conditions. In contrary, rest all the drug target gene were down-regulated, which indicates that drug severely affect DNA replication, cell proliferation, energy homeostasis, redox homeostasis and calcium homeostasis of T. evansi, leading to the death of parasite in low concentrations. It is the first attempt to investigate in vitro anti-trypanosomal activity of BC against T. evansi. These data imply that phytochemicals as alternative strategies can be explored in the future as an alternative treatment for Surra in animal.</div>","PeriodicalId":18721,"journal":{"name":"Molecular and biochemical parasitology","volume":"254 ","pages":"Article 111562"},"PeriodicalIF":1.5,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9491885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of 21 microsatellite loci for the precocious, grass-shrimp trematode Alloglossidium renale 早熟草虾异舌兰吸虫21个微卫星位点的鉴定

IF 1.5 4区医学

Molecular and biochemical parasitology Pub Date : 2023-06-01 DOI: 10.1016/j.molbiopara.2023.111563

Jenna M. Hulke, Charles D. Criscione

引用次数: 0