Characterization of the major surface glycoconjugates of Trypanosoma theileri

IF 1.4 4区医学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Molecular and biochemical parasitology Pub Date : 2023-08-29 DOI:10.1016/j.molbiopara.2023.111591

Rupa Nagar , Isobel Hambleton , Michele Tinti , Mark Carrington , Michael A.J. Ferguson

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Abstract

Trypanosoma theileri maintains a long-term extracellular infection with a low parasitaemia in bovids. The surface of this parasite is predicted to be decorated with several surface molecules including membrane surface proteases (MSPs), trans-sialidases and T. theileri putative surface proteins (TTPSPs). However, there are no experimental data to verify this hypothesis. Here, we have purified and partially characterized the surface glycoconjugates of T. theileri using biochemical and mass spectrometry-based approaches. The glycoconjugates fall into two classes: glycoproteins and glycolipids. Proteomic analysis of the glycoprotein fraction demonstrated the presence of MSPs and abundant mucin-like TTPSPs, with most predicted to be GPI-anchored. Mass spectrometric characterization of the glycolipid fraction showed that these are mannose- and galactose-containing glycoinositolphospholipids (GIPLs) that are larger and more diverse than those of its phylogenetic relative T. cruzi, containing up to 10 hexose residues and carrying either alkylacyl-phosphatidylinositol or inositol-phospho-ceramide (IPC) lipid components.

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泰勒锥虫主要表面糖缀合物的特性

泰勒锥虫在牛体内维持长期的细胞外感染，并伴有低寄生虫血症。据预测，这种寄生虫的表面会被几种表面分子修饰，包括膜表面蛋白酶（MSPs）、反式唾液酸酶和T.teileri假定的表面蛋白（TTPSP）。然而，没有实验数据来验证这一假设。在这里，我们已经使用基于生物化学和质谱的方法纯化并部分表征了T.theileri的表面糖缀合物。糖缀合物分为两类：糖蛋白和糖脂。糖蛋白组分的蛋白质组学分析表明存在MSP和丰富的粘蛋白样TTPSP，其中大多数预测为GPI锚定的。糖脂部分的质谱表征表明，这些是含有甘露糖和半乳糖的糖肌醇磷脂（GIPL），比其系统发育亲缘T.cruzi的更大、更多样，含有多达10个己糖残基，并携带烷基酰基磷脂酰肌醇或肌醇磷酸神经酰胺（IPC）脂质成分。

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来源期刊

Molecular and biochemical parasitology 医学-寄生虫学

CiteScore

2.90

自引率

0.00%

发文量

审稿时长

63 days

期刊介绍： The journal provides a medium for rapid publication of investigations of the molecular biology and biochemistry of parasitic protozoa and helminths and their interactions with both the definitive and intermediate host. The main subject areas covered are: • the structure, biosynthesis, degradation, properties and function of DNA, RNA, proteins, lipids, carbohydrates and small molecular-weight substances • intermediary metabolism and bioenergetics • drug target characterization and the mode of action of antiparasitic drugs • molecular and biochemical aspects of membrane structure and function • host-parasite relationships that focus on the parasite, particularly as related to specific parasite molecules. • analysis of genes and genome structure, function and expression • analysis of variation in parasite populations relevant to genetic exchange, pathogenesis, drug and vaccine target characterization, and drug resistance. • parasite protein trafficking, organelle biogenesis, and cellular structure especially with reference to the roles of specific molecules • parasite programmed cell death, development, and cell division at the molecular level.