Molecular & Cellular Toxicology最新文献

{"title":"Circ_101692 promotes the proliferation, invasion and glycolysis of colorectal cancer through the regulation of miR-449b-5p/GOLPH3 axis","authors":"Xiang Feng, Qian Zhang","doi":"10.1007/s13273-023-00411-9","DOIUrl":"https://doi.org/10.1007/s13273-023-00411-9","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3><p>Colorectal cancer (CRC) is one of the most common malignancies worldwide and a major threat to human life and health. Circular RNA (circRNA)-microRNA (miRNA)-mRNA mechanism is considered to occur in various cancers. However, the mechanism of circ_101692 in CRC is still unclear.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>Quantitative real-time PCR was used to detect the expression of circ_101692, miR-449b-5p and Golgi phosphoprotein 3 (GOLPH3) in CRC tissues and cell lines. Cell proliferation was determined using cell counting kit-8 (CCK8), colony formation assay and 5-ethynyl-2′-deoxyuridine (EdU). Flow cytometry and transwell were performed to measure apoptosis and cell invasion, respectively. Besides, glucose uptake and lactate production were tested using commercial kits to determine the glycolytic of CRC. Furthermore, dual-luciferase reporter assay and RNA immunoprecipitation assay were employed to verify the relationship between miR-449b-5p and circ_101692 or GOLPH3. And the protein levels were monitored using western blot assay. The xenotransplantation model was established to study the role of circ_101692 in vivo.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>Circ_101692 and GOLPH3 were highly expressed, while miR-449b-5p expression was down-regulated in CRC tissues and cell lines compared to normal tissues and cell lines. Circ_101692 negatively targeted miR-449b-5p, and GOLPH3 was the downstream gene of miR-449b-5p. Silencing circ_101692 suppressed CRC cell proliferation, invasion and glycolysis, as well as promoted apoptosis, while these influences could be reverted by miR-449b-5p inhibitor. Similarly, overexpression of GOLPH3 abolished the influence of miR-449b-5p mimic on CRC cell behavior. In addition, silencing of circ_101692 restricted CRC tumor growth in vivo.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>Our results showed that circ_101692 promoted CRC progression by modulating the miR-449b-5p/GOLPH3 axis, implying that circ_101692 might be a possible target for CRC treatment.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"53 15-18","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heme oxygenase-1 mediates the inhibitory effect of ginseng (Panax ginseng) leaf extract on differentiation in 3T3-L1 adipocytes","authors":"Hyuk Gyoon Lee, Jinwoo Hur, Jun Pil Won, Han Geuk Seo","doi":"10.1007/s13273-023-00408-4","DOIUrl":"https://doi.org/10.1007/s13273-023-00408-4","url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Background</h3><p>Obesity, which is defined as the excess accumulation of body fat, poses metabolic diseases that result in significant health risks. Since conventional anti-obesity medications are known to have significant side effects, we tried a pharmacological approach with a natural product. Ginseng (<i>Panax ginseng</i>) is a traditional Asian medicine that possesses antioxidant, anti-inflammatory, and anti-obesogenic properties. However, the mechanism of the anti-obesity effects of ginseng leaf extract (GLE) is not yet understood.</p><h3 data-test=\"abstract-sub-heading\">Objective</h3><p>We investigated the mechanism by which GLE inhibits the differentiation of 3T3-L1 preadipocytes.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>GLE treatment was administered throughout the 8 days differentiation period or at three stages of adipocyte differentiation (early: days 0–2; intermediate: days 2–4; or late: after day 4). During adipocyte differentiation, GLE treatment significantly inhibited 3T3-L1 preadipocyte differentiation at the early stage, leading to a notable reduction in lipid accumulation and a decrease in the expression of crucial adipogenic transcription factors that regulate adipocyte differentiation. GLE also increased the expression of HO-1 and Wnt/β-catenin signaling in a dose-dependent manner during adipocyte differentiation. To evaluate the role of HO-1 induced by GLE, we used HO-1 inhibitor SnPP and HO-1 siRNA. Attenuation of HO-1 function and expression inhibited the decrease in lipid accumulation and adipogenic transcription factor expression caused by GLE; furthermore, inhibition of HO-1 suppressed Wnt/β-catenin signaling.</p><h3 data-test=\"abstract-sub-heading\">Conclusions</h3><p>Overall, our results suggest that GLE inhibits the differentiation of 3T3-L1 preadipocytes by regulating HO-1 expression and Wnt/β-catenin signaling. Therefore, GLE could have preventive uses as a natural product for the treatment of obesity.</p>","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"15 1","pages":""},"PeriodicalIF":1.7,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138513842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neuregulin-1 suppresses anti-apoptotic effect of Der p 38 on neutrophils by inhibition of cytokine secretion","authors":"Yujin Hong, Ji Hyeon Choi, Min Hwa Hong, Geunyeong Kim, Ji-Sook Lee, Ran-Sook Woo, Eun Ju Yang, In Sik Kim","doi":"10.1007/s13273-023-00375-w","DOIUrl":"https://doi.org/10.1007/s13273-023-00375-w","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"33 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134954636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Claudin-5 overexpression correlates with proliferation and migration in gastric cancer","authors":"Sandang Li, Taizhe Zhang, Fuchen Xie, Zhaohui Du, Jie Du, Zhenjie Wang","doi":"10.1007/s13273-023-00407-5","DOIUrl":"https://doi.org/10.1007/s13273-023-00407-5","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"19 23","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135043267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"HDAC3 deacetylates H3K27ac and H3K9ac on the TrkC promoter to exacerbate sevoflurane-induced neurotoxicity","authors":"Jiegang Zhou, Xinwei Feng, Dan Wang","doi":"10.1007/s13273-023-00394-7","DOIUrl":"https://doi.org/10.1007/s13273-023-00394-7","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":" 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135240781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alveolar damage and development of emphysema in rats with carbon monoxide poisoning","authors":"Seok Jeong Lee, Taeyeong Kim, Yong Sung Cha, Min Kim, Ji Yong Lee, Won-Yeon Lee","doi":"10.1007/s13273-023-00405-7","DOIUrl":"https://doi.org/10.1007/s13273-023-00405-7","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135773321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of CREB1 dysregulation in calcium oxalate monohydrate crystals-induced tubular epithelial cell injury","authors":"Anqing Yang, Jianian Zhang, Tao Huang, Zhongwei Yu, Chenhui Zhao, Yongzhi Xu, Binbin Ma","doi":"10.1007/s13273-023-00404-8","DOIUrl":"https://doi.org/10.1007/s13273-023-00404-8","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"107 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135933581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carcinogenicity assessment of industrial chemicals 1,2-dichlorobenzene, 2-methoxyethanol and anisol via Bhas 42 cell transformation assay","authors":"Cheol Hong Lim, Dong Seok Seo","doi":"10.1007/s13273-023-00402-w","DOIUrl":"https://doi.org/10.1007/s13273-023-00402-w","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135992945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scallop-derived plasmalogen attenuates amyloid beta-induced inflammation and apoptosis in SH-SY5Y cells","authors":"Jin-Young Han, Miey Park, Hae-Jeung Lee","doi":"10.1007/s13273-023-00399-2","DOIUrl":"https://doi.org/10.1007/s13273-023-00399-2","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fucoidan ameliorates amyloid-β 42 oligomer-induced neuronal apoptosis by activating the PI3K/Akt signaling pathway and MAPK cascades in human neuroblastoma SH-SY5Y cells","authors":"Chae-Won Kang, Miey Park, Hae-Jeung Lee","doi":"10.1007/s13273-023-00401-x","DOIUrl":"https://doi.org/10.1007/s13273-023-00401-x","url":null,"abstract":"","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136184553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}