{"title":"长基因间非蛋白编码 RNA 1436 通过 microRNA-513a-5p/CUG 三重重复结合蛋白 1 轴激活磷脂酰肌醇 3- 激酶/Akt 信号通路，加速 MUM-2B 细胞系的细胞增殖、迁移和粘附特性","authors":"XinCheng Sun, ZhaoDong Chu, XiaoYan Zhao, MengJiao Wang, YaNan Kong, GuoHua Lu","doi":"10.1007/s13273-024-00466-2","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Objective</h3><p>Long intergenic non-protein coding RNA 1436 (LINC01436) has been reported as a critical regulator of tumor occurrence, but the association between LINC01436 and choroidal melanoma (CM) has hardly been explored. The study was to uncover the precise association of LINC01436 with CM.</p><h3 data-test=\"abstract-sub-heading\">Methods</h3><p>CM tissues and their corresponding adjacent normal tissues were collected, normal chorocytes UM96 and CM cell lines (C918, MUM-2B, OCM-1, MUM-2C) were cultured. LINC01436, microRNA (miR)-513a-5p, and CUG Triplet repeat-binding protein 1 (CELF1) expression in CM tissues and cells were detected. The screened MUM-2B cell line was transfected with LINC01436, miR-513a-5p or CELF1-related sequences or plasmids to explore their biological effects on cells with the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. The expressions of PI3K/Akt pathway-related protein were measured after transfection, same as the proliferation, cell cycle distribution, apoptosis, adhesion, migration, and invasion of MUM-2B cells. The binding of LINC01436 with miR-513a-5p and the targeting of miR-513a-5p with CELF1 were verified.</p><h3 data-test=\"abstract-sub-heading\">Results</h3><p>LINC01436 and CELF1 were upregulated in CM tissues and cells, while miR-513a-5p was reduced. LINC01436 adsorbed miR-513a-5p as a sponge, which targeted CELF1. Silenced LINC01436 suppressed the activation of PI3K/Akt pathway in MUM-2B cells as well as cell proliferation, adhesion, and metastasis. Meanwhile, elevated LINC01436 showed the opposite effect on MUM-2B cells. Restoring miR-513a-5p reversed the influence of augmented LINC01436 on MUM-2B cells. MiR-513a-5p restrained the activation of PI3K/Akt pathway and malignant behaviors of MUM-2B cells via targeting CELF1.</p><h3 data-test=\"abstract-sub-heading\">Conclusion</h3><p>LINC01436 promotes cell proliferation, migration, and adhesion properties of the MUM-2B cell line via miR-513a-5p/CELF1 axis, which may be related to the PI3K/Akt pathway.</p>","PeriodicalId":18683,"journal":{"name":"Molecular & Cellular Toxicology","volume":"63 1","pages":""},"PeriodicalIF":1.1000,"publicationDate":"2024-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Long intergenic non-protein coding RNA 1436 accelerates cell proliferation, migration and adhesion properties of the MUM-2B cell line via microRNA-513a-5p/CUG Triplet repeat-binding protein 1 axis to activate the phosphatidylinositol 3-kinase/Akt signaling pathway\",\"authors\":\"XinCheng Sun, ZhaoDong Chu, XiaoYan Zhao, MengJiao Wang, YaNan Kong, GuoHua Lu\",\"doi\":\"10.1007/s13273-024-00466-2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<h3 data-test=\\\"abstract-sub-heading\\\">Objective</h3><p>Long intergenic non-protein coding RNA 1436 (LINC01436) has been reported as a critical regulator of tumor occurrence, but the association between LINC01436 and choroidal melanoma (CM) has hardly been explored. The study was to uncover the precise association of LINC01436 with CM.</p><h3 data-test=\\\"abstract-sub-heading\\\">Methods</h3><p>CM tissues and their corresponding adjacent normal tissues were collected, normal chorocytes UM96 and CM cell lines (C918, MUM-2B, OCM-1, MUM-2C) were cultured. LINC01436, microRNA (miR)-513a-5p, and CUG Triplet repeat-binding protein 1 (CELF1) expression in CM tissues and cells were detected. The screened MUM-2B cell line was transfected with LINC01436, miR-513a-5p or CELF1-related sequences or plasmids to explore their biological effects on cells with the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. The expressions of PI3K/Akt pathway-related protein were measured after transfection, same as the proliferation, cell cycle distribution, apoptosis, adhesion, migration, and invasion of MUM-2B cells. The binding of LINC01436 with miR-513a-5p and the targeting of miR-513a-5p with CELF1 were verified.</p><h3 data-test=\\\"abstract-sub-heading\\\">Results</h3><p>LINC01436 and CELF1 were upregulated in CM tissues and cells, while miR-513a-5p was reduced. LINC01436 adsorbed miR-513a-5p as a sponge, which targeted CELF1. Silenced LINC01436 suppressed the activation of PI3K/Akt pathway in MUM-2B cells as well as cell proliferation, adhesion, and metastasis. Meanwhile, elevated LINC01436 showed the opposite effect on MUM-2B cells. Restoring miR-513a-5p reversed the influence of augmented LINC01436 on MUM-2B cells. MiR-513a-5p restrained the activation of PI3K/Akt pathway and malignant behaviors of MUM-2B cells via targeting CELF1.</p><h3 data-test=\\\"abstract-sub-heading\\\">Conclusion</h3><p>LINC01436 promotes cell proliferation, migration, and adhesion properties of the MUM-2B cell line via miR-513a-5p/CELF1 axis, which may be related to the PI3K/Akt pathway.</p>\",\"PeriodicalId\":18683,\"journal\":{\"name\":\"Molecular & Cellular Toxicology\",\"volume\":\"63 1\",\"pages\":\"\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2024-06-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecular & Cellular Toxicology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1007/s13273-024-00466-2\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"TOXICOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular & Cellular Toxicology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s13273-024-00466-2","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"TOXICOLOGY","Score":null,"Total":0}
Long intergenic non-protein coding RNA 1436 accelerates cell proliferation, migration and adhesion properties of the MUM-2B cell line via microRNA-513a-5p/CUG Triplet repeat-binding protein 1 axis to activate the phosphatidylinositol 3-kinase/Akt signaling pathway
Objective
Long intergenic non-protein coding RNA 1436 (LINC01436) has been reported as a critical regulator of tumor occurrence, but the association between LINC01436 and choroidal melanoma (CM) has hardly been explored. The study was to uncover the precise association of LINC01436 with CM.
Methods
CM tissues and their corresponding adjacent normal tissues were collected, normal chorocytes UM96 and CM cell lines (C918, MUM-2B, OCM-1, MUM-2C) were cultured. LINC01436, microRNA (miR)-513a-5p, and CUG Triplet repeat-binding protein 1 (CELF1) expression in CM tissues and cells were detected. The screened MUM-2B cell line was transfected with LINC01436, miR-513a-5p or CELF1-related sequences or plasmids to explore their biological effects on cells with the involvement of the phosphatidylinositol 3-kinase (PI3K)/Akt pathway. The expressions of PI3K/Akt pathway-related protein were measured after transfection, same as the proliferation, cell cycle distribution, apoptosis, adhesion, migration, and invasion of MUM-2B cells. The binding of LINC01436 with miR-513a-5p and the targeting of miR-513a-5p with CELF1 were verified.
Results
LINC01436 and CELF1 were upregulated in CM tissues and cells, while miR-513a-5p was reduced. LINC01436 adsorbed miR-513a-5p as a sponge, which targeted CELF1. Silenced LINC01436 suppressed the activation of PI3K/Akt pathway in MUM-2B cells as well as cell proliferation, adhesion, and metastasis. Meanwhile, elevated LINC01436 showed the opposite effect on MUM-2B cells. Restoring miR-513a-5p reversed the influence of augmented LINC01436 on MUM-2B cells. MiR-513a-5p restrained the activation of PI3K/Akt pathway and malignant behaviors of MUM-2B cells via targeting CELF1.
Conclusion
LINC01436 promotes cell proliferation, migration, and adhesion properties of the MUM-2B cell line via miR-513a-5p/CELF1 axis, which may be related to the PI3K/Akt pathway.
期刊介绍：
Molecular & Cellular Toxicology publishes original research and reviews in all areas of the complex interaction between the cell´s genome (the sum of all genes within the chromosome), chemicals in the environment, and disease. Acceptable manuscripts are the ones that deal with some topics of environmental contaminants, including those that lie in the domains of analytical chemistry, biochemistry, pharmacology and toxicology with the aspects of molecular and cellular levels. Emphasis will be placed on toxic effects observed at relevant genomics and proteomics, which have direct impact on drug development, environment health, food safety, preventive medicine, and forensic medicine. The journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.
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