Ministry of Science and Technology, Vietnam最新文献_第4页

Building a model to evaluate hypertensive crisis in mice 建立评估小鼠高血压危象的模型

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).20-25

Xuan Phuc Nguyen, Van Vien Doan, Manh Hung Tran

引用次数: 0

Identification of genetic relationships of Acacia auriculiformis clones using ISSR markers 利用 ISSR 标记鉴定金合欢克隆的遗传关系

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).55-59

Van Doan Duong, Thi Thu Ha Tran, Tri-Thuc Bui, Manh Tuan Nguyen, Tien Dung Nguyen

引用次数: 0

Expression and purification of truncated recombinant human angiotensin converting enzyme 2 (trhACE2) capable of binding spike protein of SARS-CoV-2 表达和纯化能与 SARS-CoV-2 的尖峰蛋白结合的截短重组人血管紧张素转换酶 2 (trhACE2)

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).08-13

Thi Le Thuy Nguyen, Minh Tuan Nguyen, Thi Kim Tram Pham, Dang Quan Nguyen

{"title":"Expression and purification of truncated recombinant human angiotensin converting enzyme 2 (trhACE2) capable of binding spike protein of SARS-CoV-2","authors":"Thi Le Thuy Nguyen, Minh Tuan Nguyen, Thi Kim Tram Pham, Dang Quan Nguyen","doi":"10.31276/vjst.66(2).08-13","DOIUrl":"https://doi.org/10.31276/vjst.66(2).08-13","url":null,"abstract":"SARS-CoV-2 infects the host through the interaction be-tween spike protein and the ACE2 (Angiotensin convert-ing enzyme 2) receptor on the host cell surface. There-fore, ACE2 is considered as a key target in drug devel-opment against COVID-19. In this study, we generated the truncated recombinant human ACE2 (trhACE2) expressed on Escherichia coli and evaluated its binding activity to spike protein of SARS-CoV-2. The sequence of ACE2 (18-119 aa) was cloned into pET28a(+) plasmid and transformed into E. coli DH5α strain and finally was transferred to E. coli BL21 (DE3) cells for protein expression. Protein trhACE2 was purified by affinity chromatography using a HisTrap HP column. Protein purity was above 95% and production yield was 75 mg/l. The purified protein was refolded by dialysis method. trhACE2 was evaluated for its ability to bind the spike protein by sandwich ELISA assay and surface plasmon resonance (SPR). The results showed that trhACE2 has the ability to bind spike protein of SARS-CoV-2 with the equilibrium dissociation constant Kd=15.7 nM. With a relatively simple and inexpensive expression and purifi-cation process on E. coli system, there is the potential to develop trhACE2 for preventive and supportive thera-pies against COVID-19.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"14 75","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of collagen membrane from amniotic membrane as a substrate for adherent cell culturing in tissue engineering 将羊膜胶原蛋白膜作为粘附细胞培养基质在组织工程中的应用

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).26-31

Thanh Binh Nguyen, Thi Thu Hien Nguyen, Phuong Thanh Duong, Thi Thanh Thuy Tran, Ngoc Hien Nhan, Khanh Hoa Nguyen, KC Huong Hoang, Tuan Kiet Nguyen, Minh Can Nguyen, T. Q. Ngo, Duy Thao Huynh

{"title":"Application of collagen membrane from amniotic membrane as a substrate for adherent cell culturing in tissue engineering","authors":"Thanh Binh Nguyen, Thi Thu Hien Nguyen, Phuong Thanh Duong, Thi Thanh Thuy Tran, Ngoc Hien Nhan, Khanh Hoa Nguyen, KC Huong Hoang, Tuan Kiet Nguyen, Minh Can Nguyen, T. Q. Ngo, Duy Thao Huynh","doi":"10.31276/vjst.66(2).26-31","DOIUrl":"https://doi.org/10.31276/vjst.66(2).26-31","url":null,"abstract":"The human amniotic membrane has been widely used in clinical practice to treat all kinds of lesions related to the epithelial surface, corneal epithelial reconstruction, and orthopaedic trauma. Currently, another application aspect of the amniotic membrane of great interest is its use as an in vitro adherent cell culture medium. In this study, the human amniotic membranes were collected under sterile conditions in the operating room and tested negative for HIV, HBV, HCV, and VDRL. The amniotic membranes were then processed to remove the epithelial layer, to obtain a collagen membrane that can be used as a substrate for cell culture adhesion. Following that, the collagen membrane was structurally evaluated by Hematoxylin and eosin (H&E) staining, SEM. Next, we tested the use of collagen membranes as a culture medium for human fibroblasts and evaluated cell adhesion, growth, and development by histological images over time of cell culture. The image results obtained before and after processing showed that the collagen membrane has an acellular structure, maintaining the basement membrane’s structure. Cell culture results exhibited that fibroblasts adhered and developed well on this collagen membrane.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genotype identification and genetic diversity of the ompA gene among Chlamydia trachomatis 沙眼衣原体中 ompA 基因的基因型鉴定和遗传多样性

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).01-07

Hoa Nguyen, Van Du Vu, Thi Nhu Quynh Nguyen, Le Van Nguyen, Ngoc Anh Do

{"title":"Genotype identification and genetic diversity of the ompA gene among Chlamydia trachomatis","authors":"Hoa Nguyen, Van Du Vu, Thi Nhu Quynh Nguyen, Le Van Nguyen, Ngoc Anh Do","doi":"10.31276/vjst.66(2).01-07","DOIUrl":"https://doi.org/10.31276/vjst.66(2).01-07","url":null,"abstract":"This study aimed to identify the distribution and analysis of genetic diversity of Chlamydia trachomatisgenotypes in infertile Vietnamese women. A total of 119 endocervical swabs were isolated from infertile women at the National Hospital of Obstetrics and Gynecology, Vietnam, during the period from January 2020 to December 2021, and was diagnosed with Chlamydiatrachomatis infection. 81 sequences of the ompA gene with a size of approximately 1,100 bp were used to determine the Chlamydia trachomatis genotypes by the currently reference sequences from GenBank. Sequence analysis of the ompA gene from the 81 positive patients showed nine genotypes, including E, D/Da, F, G/Ga, J, H, K, B/Ba, and I/Ia. In that, E, D/Da, and F were most prevalent (61.74%). Genotypes E, F, and G/Ga were highly conserved, showing 100% similarity to the alternative reference sequences, whereas ompA sequences of genotypes B/Ba, D/Da, H, I/Ia, J, and K displayed from one to ten nucleotide substitutions. In total, nineteen nucleotide changes were detected, seven of which led to amino-acid substitutions. The findings of the current study showed high prevalence of the genotypes E, D/Da, and F of C. trachomatis in infertile Vietnamese women.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"25 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pollen fertility at different flowering times and its effect on seed setting of some bitter gourd (Momordica charantia L.) varieties in Ho Chi Minh city 胡志明市一些苦瓜（Momordica charantia L.）品种不同花期的花粉育性及其对结籽的影响

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).39-43

Dang Thai Phuong Phan, Thuy Duong Nguyen, Van Ba Vu

{"title":"Pollen fertility at different flowering times and its effect on seed setting of some bitter gourd (Momordica charantia L.) varieties in Ho Chi Minh city","authors":"Dang Thai Phuong Phan, Thuy Duong Nguyen, Van Ba Vu","doi":"10.31276/vjst.66(2).39-43","DOIUrl":"https://doi.org/10.31276/vjst.66(2).39-43","url":null,"abstract":"In the hybridisation process, pollen grains directly participate in the process through flower pollination. Pollen grain quality determines fruit formation and seed setting. In this study, the pollen grains of the local bitter gourd variety (BD2) and F1 commercial variety were collected at 8 different flowering times (8-11h30) for fertility evaluation in the 2020 Spring-Winter crop season in Ho Chi Minh city. Self-pollinate the 2 varieties over 8-9 hours to evaluate their ability to set seeds. The obtained results indicated that the local variety (BD2) had the highest fertility pollen (61.26%) at 08:30 am and the lowest (46.22%) at 9:00 am. Meanwhile, the F1 commercial variety showed the highest fertility pollen (94.05%) at 11:30 am and the lowest (79.58%) at 8:00 am. The fertility of variety BD2 is 60.21%, resulting in 33.50 seeds per fruit in which the percentage of mature seed is 71.72%. Similarly, the commercial variety found the pollen fertility is 85.55%, resulting in 11.25 seeds per fruit in which the percentage of mature seeds is 84.90%. This information is useful for bitter gourd hybridisation.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"35 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation of Phyllanthus amarus under Clinostat 2D condition 在 Clinostat 2D 条件下，白花蛇舌草的体外形态发生、抗氧化酶活性和次生化合物积累

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).49-54

The Bien Le, T. Hoang, Thi Nhu Mai Nguyen, Dac Khai Hoang, Manh Cuong Do, Hoai Phong Truong, Quoc-Luan Vu, Van The Vinh Bui, T. Duong

{"title":"In vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation of Phyllanthus amarus under Clinostat 2D condition","authors":"The Bien Le, T. Hoang, Thi Nhu Mai Nguyen, Dac Khai Hoang, Manh Cuong Do, Hoai Phong Truong, Quoc-Luan Vu, Van The Vinh Bui, T. Duong","doi":"10.31276/vjst.66(2).49-54","DOIUrl":"https://doi.org/10.31276/vjst.66(2).49-54","url":null,"abstract":"In this study, 4-week-old internodes of in vitroPhyllanthus amarus with 1 cm in length were cut in a half longitudinal section and cultured under 2D clinostat and control conditions to evaluate in vitro morphogenesis, antioxidant enzyme activity and secondary compound accumulation. The results showed that 34.33% of explants induced callus and 65.67% of explants formed adventitious root under 2D clinostat compared with 100% explants induced callus under control after 4 weeks of culture. In addition, the fresh and dry weights of callus clusters (792.00 and 79.17 mg, respectively) under 2D clinostat were higher than those under control (313.33 and 30.07 mg, respectively). Superoxide dismutase (SOD), catalase (CAT), and phenolic under 2D clinostat were higher than those of control. For hypophyllanthin and phyllanthin, the concentrations in the callus under 2D clinostat condition were higher than control. The hypophyllanthin content in the callus (29.06 μg/g fresh weight) was higher than in the adventitious root (9.03 μg/g fresh weight) under 2D clinostat, while the phyllanthin only obtained in the callus that was absent in the adventitious root.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"28 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140431961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Initial evaluation of the application of autologous protein gel in the treatment of extensive skin loss wounds 应用自体蛋白凝胶治疗大面积皮肤缺损伤口的初步评估

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).32-38

Quang Tri Le, H. Phan, Thi Thanh Tam Vu, Gia Huy Truong, Nguyen Nhu Hieu Tran, Thi Dieu Thuan Hoang, Le Bao Ha Tran, Minh Quan To, Thi Ngoc My Nguyen, Nguyen Vu Doan

引用次数: 0

Evaluation of the results of the modified Blumgartpancreatic-intestinal anastomosis technique according to Satoi in pancreaticoduodenectomy at Bach Mai Hospital 对巴赫迈医院在胰十二指肠切除术中采用里氏改良布隆加特胰肠吻合技术的效果评估

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).14-19

Pisey Chantha, Thanh Khiem Nguyen, Ham Hoi Nguyen, N. H. Nguyen, Hieu Hoc Tran, Tuan Hiep Luong, Van Minh Do, Hai Dang Do, The Duy Nguyen, Hong Quang Pham

{"title":"Evaluation of the results of the modified Blumgartpancreatic-intestinal anastomosis technique according to Satoi in pancreaticoduodenectomy at Bach Mai Hospital","authors":"Pisey Chantha, Thanh Khiem Nguyen, Ham Hoi Nguyen, N. H. Nguyen, Hieu Hoc Tran, Tuan Hiep Luong, Van Minh Do, Hai Dang Do, The Duy Nguyen, Hong Quang Pham","doi":"10.31276/vjst.66(2).14-19","DOIUrl":"https://doi.org/10.31276/vjst.66(2).14-19","url":null,"abstract":"Objectives: This study aims to research on the safety and effectiveness of the modified Blumgart pancreaticojejunostomy anastomosis by Satoi in Bach Mai Hospital. Research subjects: Seventy-three patients underwent modified Blumgart pancreaticojejunostomy anastomosis by Satoi after pancreaticoduodenectomy at Gastrointestinal and Hepato-Biliary-Pancreatic Surgery Department, Bach Mai Hospital between October 2020 and April 2023. Method: Cross-sectional study combining retrospective and prospective studies. The Blumgart anastomosis was modified to simplify with 2 U-shaped sutures. Results: The average age of the study subjects was 58.49±12.69 years; the age group over 60 accounted for over 50%; male/female ratio was ~1. The number of patients with complications was 22/73; the total number of complications was 33, including 3 grade-III-or-above complications, according to Clavien-Dindo: 1 case had complications of grade C pancreatic fistula causing late hemorrhage, from the common hepatic artery wall, needs re-surgery, 1 case needs hemostasis under intervention radiology, 1 case needs to be drained under ultrasound guidance, and no patient deaths. Conclusion: The modified Blumgart pancreaticojejunostomy by Satoi is a safe and effective technique.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"43 19","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro rapid multiplication of two ancient gerbera varieties (Gerbera jamesonii Bolus) 两个古老非洲菊品种（Gerbera jamesonii Bolus）的体外快速繁殖

Ministry of Science and Technology, Vietnam Pub Date : 2024-02-25 DOI: 10.31276/vjst.66(2).66-74

Thi Thu Hien Phan, Thi Kim Anh Nguyen, T. Le, Thi Thuy Hang Vu

{"title":"In vitro rapid multiplication of two ancient gerbera varieties (Gerbera jamesonii Bolus)","authors":"Thi Thu Hien Phan, Thi Kim Anh Nguyen, T. Le, Thi Thuy Hang Vu","doi":"10.31276/vjst.66(2).66-74","DOIUrl":"https://doi.org/10.31276/vjst.66(2).66-74","url":null,"abstract":"Gerbera buds are double sterilised, the clean sample rate is up to 90% (ancient yellow gerbera daisies) and 92% (ancient pink gerbera daisies). With the medium supplemented with 1.5 mg/l BAP and 2 mg/l TDZ, the yellow gerbera daisy variety obtained a regeneration rate of 52.17%, the number of regenerated buds was 5 buds/sample. Ancient pink gerbera daisies have a regeneration rate of 53.85%, the number of regenerated buds was 6 buds/sample. When using 1.5 mg/l IBA combined with 2 mg/l BA, the gerbera daisies obtained a bud formation rate of 44.45% with an average number of 5 buds/sample; ancient pink gerbera daisies achieved a high bud formation rate of 46.15%, with an average number of 6 buds/sample. Media with 0.05 mg/l NAA and 0.3 mg/l IBA produced 6-7 roots, about 2.5 cm in length; the temperature of 20-25oC was most suitable for rooting in 2 varieties of gerbera daisy. After forming roots, the plantlets were planted in a substrate consisting of 50% sand + 30% soil + 20% smoked rice husks. The survival rate after 8 weeks and the height of the ancient yellow gerbera daisies and ancient pink gerbera daisies reached 98.26%, 14.8 cm, 99.05%, and 12.6 cm, respectively.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"19 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0