{"title":"Antifungal activity of a methanolic extract of Glycyrrhiza uralensis Fisch. root against Fusarium oxysporum and Phytophthora capsici","authors":"Dang-Minh-Chanh Nguyen, Thi-Hoan Luong, Van-Viet Nguyen, Woo-Jin Jung","doi":"10.31276/vjste.66(1).46-52","DOIUrl":"https://doi.org/10.31276/vjste.66(1).46-52","url":null,"abstract":"In the present study, we investigated the antifungal activity of a methanolic extract of Glycyrrhiza uralensisFisch. root, which inhibited the growth of the plant pathogens Fusarium oxysporum and Phytophthora capsici. The EC50 values of the G. uralensis Fisch. extract three, six, and nine days after treatment were 113.4, 150.6, and 191.4 μg/ml for F. oxysporum and 84.7, 148.6, and 190.1 μg/ml for P. capsici, respectively, and the antifungal activity exhibited a dose-dependent response. The MIC value of G. uralensis Fisch. extract was found to be 100, 200, and 400 μg/ml at three, six, and nine days after treatment for F. oxysporum. In contrast, for P. capsici, the MIC value was 50, 100, and 100 μg/ml at three, six, and nine days after treatment. After three days of incubation with 400 μg/ml G. uralensis Fisch. extract, the hyphae of F. oxysporum exhibited abnormalities and atrophy, leading to mycelial collapse. In contrast, the hyphae of P. capsici displayed swelling with increased branching. These results furnish valuable insights into the antifungal metabolites present in G. uralensis Fisch. extract, contributing to the development of environmentally friendly products aimed at controlling the growth of pathogenic fungi.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"55 S1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140238533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Linh-Thy Le, Khanh-Bang V. Quang, Trieu-Vi Vo, Thanh-Mai T. Nguyen, Thi-Viet-Huong Dao, Xuan-Thanh Bui
{"title":"Environmental and health impacts of air pollution: A mini-review","authors":"Linh-Thy Le, Khanh-Bang V. Quang, Trieu-Vi Vo, Thanh-Mai T. Nguyen, Thi-Viet-Huong Dao, Xuan-Thanh Bui","doi":"10.31276/vjste.66(1).120-128","DOIUrl":"https://doi.org/10.31276/vjste.66(1).120-128","url":null,"abstract":"Air pollution is one of the leading risk factors for death but also a significant contributor to the global disease burden, affecting quality of life. According to a World Health Organisation (WHO) report, seven million people die from air pollution every year, and 9 out of 10 people worldwide breathe polluted air. Any person can be affected by exposure to polluted air, especially the elderly, children, pregnant women, and people with comorbidities. Some studies indicate that the diseases most affected by air pollution are respiratory infections, chronic obstructive pulmonary disease (COPD), lung cancer, and cardiovascular diseases. The degree of effect on the body depends on the pollutant composition, source and dose, level and duration of exposure to polluted air. Particulate matter (PM10 and PM2.5), carbon monoxide (CO), ozone (O3), nitrogen dioxide (NO2), and sulphur dioxide (SO2) could lead to air pollution. Long-term exposure to air pollution can affect every organ in the body and worsen existing health conditions. Short-term exposure to contaminants can include unpleasant sensations such as coughing, wheezing, shortness of breath, eye, nose, and throat irritation, headache, dizziness, and fatigue. Community and individual solutions such as using clean fuel, wearing personal masks, filtering indoor air, and ventilating need to be taken to reduce the impact of air pollution.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"27 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140239970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minh Hieu Vu, Doan Hong Ngoc Tran, Minh-Anh Dang-Trinh, Huynh Chan Khon
{"title":"Development of a lateral flow immunoassay with HRP enhancement for spiked SARS-CoV-2 protein N detection in human saliva","authors":"Minh Hieu Vu, Doan Hong Ngoc Tran, Minh-Anh Dang-Trinh, Huynh Chan Khon","doi":"10.31276/vjste.66(1).111-119","DOIUrl":"https://doi.org/10.31276/vjste.66(1).111-119","url":null,"abstract":"The Coronavirus (SARS-CoV-2) is an enveloped RNA virus that caused a dangerous COVID-19 pandemic following an outbreak in Wuhan, China in 2019. In response to the pandemic, the development of lateral flow assays (LFAs) has been crucial for the detection of viruses, commonly targeting the spike (S) or nucleocapsid (N) protein in nasopharyngeal swab (NS) specimens. COVID-19, caused by the SARS-CoV-2 virus, predominantly manifests as a respiratory tract infection-like illness, characterized by symptoms such as fever, dry cough, upper airway congestion, runny nose, sore throat, myalgia, headache, and exhaustion. This study presents the development of an LFA targeting the N protein to detect the coronavirus in saliva specimens, using a sandwich format. The use of 80 nm gold nanoparticles (AuNPs) has been investigated, and the application of horseradish peroxidase (HRP) and TMB substrate has been employed to enhance the limit of detection (LOD). The results demonstrate a significant 200-fold improvement in LOD, from 10 to 50 pg/ml, for N protein spiked in saliva samples after the application of HRP-TMB. This finding highlights an important advancement towards the utilization of saliva samples in diagnostic applications.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"123 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chinh Nghia Nguyen, Hang Ngan Dinh, Thu Trang Nguyen, Ha Trang Nguyen, Thi Trang Nguyen, Giang Hoang, Ky Son Chu, Thu Trang Vu
{"title":"Factors affecting the coagulation of milk protein during quark cheese processing in Vietnam","authors":"Chinh Nghia Nguyen, Hang Ngan Dinh, Thu Trang Nguyen, Ha Trang Nguyen, Thi Trang Nguyen, Giang Hoang, Ky Son Chu, Thu Trang Vu","doi":"10.31276/vjste.66(1).104-110","DOIUrl":"https://doi.org/10.31276/vjste.66(1).104-110","url":null,"abstract":"In recent years, cheese consumption in Vietnam is markedly rising, leading to the attention of local milk processors on cheese making. Quark-type cheese, a traditional food in Central Europe, is a type of well-known acid-coagulated cheese product. Milk coagulation properties (MCPs) play an important role to the success of the cheese-making process and are influenced by different parameters. In this study, the quality of raw milk for cheese making and the coagulation conditions in quark-type cheese processing were investigated. Raw milk collected from Phu Dong dairy farm met the requirement of Vietnamese standard for cheese making. The appropriate conditions for coagulation were identified: the heat treatment of milk at 60°C for 15 minutes, coagulation temperature at 40oC and pH 5.5, CaCl2 concentration at 0.04g/l. Under these conditions, a short coagulation time (72.33 seconds) and high curd yields (50.70%) were obtained. These results suggested that quark-type cheese using raw milk in Vietnam could be successfully developed at larger scale.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"25 22","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140240263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Analysis of hydraulic drive circuit selection for a carrot collecting system","authors":"Ngoc Danh Dang, Xuan Thiet Nguyen","doi":"10.31276/vjste.66(1).16-23","DOIUrl":"https://doi.org/10.31276/vjste.66(1).16-23","url":null,"abstract":"Compared to conventional mechanical transmissions, hydraulic transmission has the disadvantage of lower energy efficiency. However, it is widely employed in various working machines such as construction machines, off-road vehicles, forest logging machines, and especially agricultural machinery, owing to its flexibility in design, geometric arrangement, and simplicity in operation. In the development project for carrot harvesting machinery conjugate, hydraulic transmission is the optimal solution for the design and implementation of the carrot collecting roller system. This project is oriented towards achieving a practical result where the system structure is simple and effective, utilising hydraulic components available on the market in Vietnam. Given the myriad of hydraulic transmission circuits available, this paper undertakes an analysis based on numerical simulations of the functionality and energy efficiency of potential system design options and proposes a new practical speed control circuit. This suggested circuit uses a pressure-compensated flow control valve arranged parallel to the hydraulic motor. The study is carried out in the Matlab/Simulink simulation environment, where the circuit models are constructed using hydraulic components with practical parameter settings sourced from component suppliers. The analysis results indicate that the proposed circuit strikes a balance between functionality and economic efficiency.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"37 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"RNA isolation by two different methods in analysing adipose-biomarkers of mouse adipose tissues","authors":"D. Chu, Hue Vu Thi, Ngoc Hoan Le","doi":"10.31276/vjste.66(1).84-89","DOIUrl":"https://doi.org/10.31276/vjste.66(1).84-89","url":null,"abstract":"The RNeasy Mini Kit and the TRI Reagent protocol are commonly employed techniques for extracting total RNA and are widely utilised in research pertaining to the expression of adipose biomarkers. This study was undertaken to establish and determine an appropriate method for isolating RNA from frozen adipose tissue, with the objective of analysing adipose biomarkers. Total RNA was extracted from frozen white adipose tissues of mice (-80oC) using both the RNeasy Mini Kit and the TRI Reagent protocol. The concentration and purity of the RNA were assessed using NanoDrop. One-step RT-qPCR was employed to quantify the mRNA expression levels of three adipose biomarkers: MEST, SFRP5, and SCD1. The results of this investigation indicated that the RNeasy Mini Kit yielded a higher RNA concentration when compared to the TRI Reagent method. Furthermore, the RNeasy Mini Kit provided RNA samples with superior purity, as evidenced by a higher 260/230 ratio. Although RNA extracted using the TRI Reagent exhibited slightly elevated mRNA expression levels for the three biomarkers, these differences did not reach statistical significance. In summary, both methods are suitable for total RNA extraction, but the RNeasy Mini Kit is recommended for obtaining higher RNA concentration and purity. The choice of method should be contingent upon the intended downstream application of the RNA.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"116 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Long Hoang, Mai T. T. Nguyen, D. N. Nguyen, Kim Hoang, Clair Hershey, R. Howeler
{"title":"Vietnamese cassava varieties progression across 50 years","authors":"Long Hoang, Mai T. T. Nguyen, D. N. Nguyen, Kim Hoang, Clair Hershey, R. Howeler","doi":"10.31276/vjste.66(1).59-76","DOIUrl":"https://doi.org/10.31276/vjste.66(1).59-76","url":null,"abstract":"Vietnamese cassava varieties constitute the fundamental and pivotal element in the sustainable development programme for cassava. This article aims to encapsulate the advancements made over nearly five decades in breeding and enhancing Vietnamese cassava varieties. It delineates the suitable cassava variety structures for each period and ecological region. The selection of cassava varieties exhibiting high starch yield and disease resistance, coupled with the establishment of a suitable and efficient cassava cultivation model, exemplified by 10T for Vietnamese cassava varieties KM568, KM539, KM537, KM569, and KM94, stands as a cornerstone for sustaining cassava development over the years. Presently, we advocate for farmers to cultivate promising cassava varieties such as KM568 or KM539 (an enhanced version of the International Center for Tropical Agriculture (CIAT) cassava variety C39, refined through multiple breeding cycles from 2004 onwards), KM537, KM569, or HN1 (originally known as TMEB419), alongside popular cassava varieties: KM440, KM419, KM94, KM7, STB1, KM414, KM98-7, KM140, KM98-5, KM98-1. We have conducted Distinctness, Uniformity, and Stability (DUS) and Value for Cultivation and Use (VCU) tests, showcasing outstanding cassava varieties in large-scale farming, thereby providing compelling evidence for the prudent conservation and sustainable development of cassava. Vietnamese cassava progression (1975 to date) has traversed six stages, with five waves of restructuring cassava varieties, aligning with target orientations, farming conditions, and market demands, culminating in 16 popular cassava varieties and four promising cassava varieties KM568, KM539, KM537, and KM569.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"15 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140237473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Thi Thanh Nhan Giang, Thi Phuong Mai Pham, Van Ba Nguyen, Thi Thu Thuy Tran, Thi Quynh Chau Nguyen, Thi Hau Tran, T. A. Hoang, Khanh Van Nguyen, Q. M. Luu, Doan Lan Pham
{"title":"Analysis of the mitochondrial genome and phylogenetic relationships of Mong chicken","authors":"Thi Thanh Nhan Giang, Thi Phuong Mai Pham, Van Ba Nguyen, Thi Thu Thuy Tran, Thi Quynh Chau Nguyen, Thi Hau Tran, T. A. Hoang, Khanh Van Nguyen, Q. M. Luu, Doan Lan Pham","doi":"10.31276/vjst.66(2).44-48","DOIUrl":"https://doi.org/10.31276/vjst.66(2).44-48","url":null,"abstract":"In this study, the complete nucleotide sequence of the mitochondrial genome of the Mong chicken and an indigenous chicken breed of Vietnam, was determined by the Sanger sequencing method, structurally analysed and used to evaluate the phylogenetic relationship with different chicken breeds. The complete nucleotide sequence data of the Mong chicken’ mitochondrial genome was registered on GenBank with the accession number OR643716. The Mong chicken’ mitochondrial genome has a size of 16785 base pair (bp) and includes 22 transfer RNA genes, two ribosomal RNA genes, 13 protein-coding genes, and one non-coding control region (D-loop). A, T, G, and C nucleotide proportions were 30.28, 23.76, 13.48, and 32.48%, respectively. Results of the analysis of phylogenetic relationships based on 33 complete mitochondrial genome sequences showed that the Mong chicken has evolutional origin from the red junglefowl subspecies Gallus gallus spadiceus. The Mong chicken is in close relationship with the Dong Tao chicken and Cao Son dwarf chicken in Vietnam, Rugao yellow chicken and Xiaoxiang chicken in China.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"24 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Phuoc Thien Hoang Truong, Thi Thao Nhu Le, Hoang Phuc Le, Trong Nghia Tran, Uyen Tran Da Dao
{"title":"A survey on the composition and occurrence frequency of arbuscular mycorrhizal fungi in rhizospheric soils and roots of vegetables grown in Ho Chi Minh city","authors":"Phuoc Thien Hoang Truong, Thi Thao Nhu Le, Hoang Phuc Le, Trong Nghia Tran, Uyen Tran Da Dao","doi":"10.31276/vjst.66(2).60-65","DOIUrl":"https://doi.org/10.31276/vjst.66(2).60-65","url":null,"abstract":"The study aimed to determine the presence and composition of arbuscular mycorrhizal fungi (AMF) in the rhizospheric soils and roots of vegetable plants (14 species of leafy vegetables) grown in Hoc Mon, Cu Chi, and Binh Chanh districts, Ho Chi Minh city. The results of the isolation of AMF spores based on the wet sieving method showed the presence of AMF in the rhizospheric soils of vegetables with an average spore density of 87.6 spores/50 g of soil. For the symbiotic ratio of AMF to the root tissues, there was no symbiosis in 4 vegetable crops, including amaranth, mustard green, leaf mustard, and bok choy. Still, there was symbiosis in the remaining 10 plants, with an average symbiotic rate of 6.6%. Identification of AMF spores based on the morphological characteristics recorded in all the soil samples appeared spore phenotypes of 5 fungal genera including Glomus, Acaulospora, Gigaspora, Scutellospora, and Sclerocystis. However, 3 morphological types were not yet identified. From the obtained results, two genera, Glomus and Acaulospora, have the highest frequency at 43.9 and 39.6%, respectively.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"30 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140433119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minh Tuong Le, Hong Nhieu Le, Quoc Viet Le, Quang Dung Nguyen, Van Tap Nguyen
{"title":"Antagonistic potential of actinomycete strains against Colletotrichum sp. causes anthracnose disease in durian","authors":"Minh Tuong Le, Hong Nhieu Le, Quoc Viet Le, Quang Dung Nguyen, Van Tap Nguyen","doi":"10.31276/vjst.66(2).75-80","DOIUrl":"https://doi.org/10.31276/vjst.66(2).75-80","url":null,"abstract":"The research was carried out at Can Tho University with the aim of investigating the ability of actinomycetes isolates to antagonise Colletotrichum sp. fungus causing anthracnose disease in durian. The results showed that among the 28 actinomycete strains evaluated, 6 actinomycetes including BT19, BL10, TG19, BT16, ĐT15, and VL9 had stronger antagonism with a radius of inhibition zone reaches 12.4, 9.4, 11.1, 9.8, 9.7, and 9.2 mm, respectively and antagonistic efficacy of 63.22, 58.62, 55.86, 53.56, 52.64, and 53.79%, respectively at 7 days after co-inoculation. The ability to degrade β-glucan of 6 strains BT19, BL10, TG19, BT16, DT15, and VL9 performed under laboratory conditions showed that the BT19 isolate had the highest β-glucanolytic activity with the β-glucan lyse halo radius of 8.53 mm and secreted β-glucanase content reached 0.678 IU/ml at 14 days after testing. The results of this study will be the basis for further research to contribute to finding effective methods to control anthracnose disease in durian.","PeriodicalId":18650,"journal":{"name":"Ministry of Science and Technology, Vietnam","volume":"23 12","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140432104","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}