In vitro rapid multiplication of two ancient gerbera varieties (Gerbera jamesonii Bolus)

Abstract

Gerbera buds are double sterilised, the clean sample rate is up to 90% (ancient yellow gerbera daisies) and 92% (ancient pink gerbera daisies). With the medium supplemented with 1.5 mg/l BAP and 2 mg/l TDZ, the yellow gerbera daisy variety obtained a regeneration rate of 52.17%, the number of regenerated buds was 5 buds/sample. Ancient pink gerbera daisies have a regeneration rate of 53.85%, the number of regenerated buds was 6 buds/sample. When using 1.5 mg/l IBA combined with 2 mg/l BA, the gerbera daisies obtained a bud formation rate of 44.45% with an average number of 5 buds/sample; ancient pink gerbera daisies achieved a high bud formation rate of 46.15%, with an average number of 6 buds/sample. Media with 0.05 mg/l NAA and 0.3 mg/l IBA produced 6-7 roots, about 2.5 cm in length; the temperature of 20-25oC was most suitable for rooting in 2 varieties of gerbera daisy. After forming roots, the plantlets were planted in a substrate consisting of 50% sand + 30% soil + 20% smoked rice husks. The survival rate after 8 weeks and the height of the ancient yellow gerbera daisies and ancient pink gerbera daisies reached 98.26%, 14.8 cm, 99.05%, and 12.6 cm, respectively.