Methods and Objects of Chemical Analysis最新文献

筛选
英文 中文
Electrocatalytic Effect of Al2O3 Supported on Clay in Oxidizing of Ibuprofen at Graphite Electrode 粘土负载氧化铝在石墨电极上氧化布洛芬的电催化作用
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2021-01-01 DOI: 10.17721/moca.2021.81-87
Hayat El Ouafy, T. E. Ouafy, M. Oubenali, A. Haimouti, A. Gamouh, M. Mbarki
{"title":"Electrocatalytic Effect of Al2O3 Supported on Clay in Oxidizing of Ibuprofen at Graphite Electrode","authors":"Hayat El Ouafy, T. E. Ouafy, M. Oubenali, A. Haimouti, A. Gamouh, M. Mbarki","doi":"10.17721/moca.2021.81-87","DOIUrl":"https://doi.org/10.17721/moca.2021.81-87","url":null,"abstract":"In this work, the electro-catalytic oxidation of ibuprofen was studied using aluminum oxide supported on clay (Clay/Al2O3). The latter has been successfully prepared by impregnating aluminum particles in the clay by heat treatment. The electro-catalytic performances of Clay/Al2O3 for the oxidation of ibuprofen were studied using cyclic voltammetry (CV), chronoamperometry, and differential pulse voltammetry (DPV) in 0.1 mol L-1 of the phosphate buffer (pH = 7). It has been shown that the proposed catalyst exhibits remarkably an electro-catalytic effect performance vis-a-vis the oxidation of ibuprofen. In addition, the peak oxidation currents depend linearly on the ibuprofen concentration in the wide ranges from 1.0·10-3 mol L-1 to 1.0·10-6 mol L-1 with a detection limit of 1.95·10-8 mol L-1 and response time of 30 second. Possible interferences were evaluated in 1.0·10-5 mol L-1 ibuprofen. The proposed catalyst also indicated suitable repeatability and stability. Besides, the proposed CPE-Clay/Al2O3 has been successfully applied for ibuprofen analysis in human blood with good recoveries.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67436161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Mesoporous Silica With Covalently Immobilized Anthracene As Adsorbent For SPE Recovery Of PAHs Pollutants From Highly Lipidic Solutions 以共价固定化蒽为吸附剂的介孔二氧化硅固相萃取法回收高脂溶液中的多环芳烃
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2020-12-21 DOI: 10.26434/chemrxiv.13394228.v1
Albina Mikhraliieva, R. Gonçalves, V. Zaitsev
{"title":"Mesoporous Silica With Covalently Immobilized Anthracene As Adsorbent For SPE Recovery Of PAHs Pollutants From Highly Lipidic Solutions","authors":"Albina Mikhraliieva, R. Gonçalves, V. Zaitsev","doi":"10.26434/chemrxiv.13394228.v1","DOIUrl":"https://doi.org/10.26434/chemrxiv.13394228.v1","url":null,"abstract":"Two samples of functionalized mesoporous silica containing anchored anthrylmethylamine groups (SiO2-Ant) have been prepared by surface assembling (1) and one step silane immobilization (2). Both adsorbents can be attributed to bimodal balanced hydrophobic-hydrophilic adsorbents with loading of anthracene groups about 15-33 %. The adsorbents have been used for solid-phase extraction (SPE) of anthracene from organic solvents (acetonitrile, acetone and heptane) and model solutions of lipids (myristic acid and vegetable oil). The obtained results were compared with commercial C18 SPE cartridge. While C18 cartridge recovers anthracene from water-containing media (acetonitrile/water, 1/1), SiO2-Ant cartridges much more efficient in extraction of anthracene from non-polar solvent (heptane). Lipids macrocomponents such as myristic acid and vegetable oil do not decrease the dynamic adsorption capacity and recovery of the model polycyclic aromatic hydrocarbon (PAH) on SiO2-Ant. It was demonstrated that π-π stacking interaction with the analyte determine the selectivity of SiO2-Ant towards of anthracene. This makes SiO2-Ant attractive for selective preconcentration of PAHs from high lipid content objects, such as vegetable oils.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2020-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46147220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Oxidative Derivatization Method For The Spectrofluorimetric Determination Of Periciazine In Pharmaceutical Preparations 氧化衍生法测定药物制剂中哌嗪的含量
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2020-01-01 DOI: 10.17721/moca.2020.21-26
M. Blazheyevskiy, Y. Scrypynets, A. Yegorova, V. Antonovich
{"title":"The Oxidative Derivatization Method For The Spectrofluorimetric Determination Of Periciazine In Pharmaceutical Preparations","authors":"M. Blazheyevskiy, Y. Scrypynets, A. Yegorova, V. Antonovich","doi":"10.17721/moca.2020.21-26","DOIUrl":"https://doi.org/10.17721/moca.2020.21-26","url":null,"abstract":"The oxidative derivatization method for the indirect spectrofluorimetric determination of Periciazine has been presented. Potassium hydrogenperoxymonosulfate (Oxone ®) is proposed as a derivatizing agent for Periciazine, yielding the strongly fluorescent Periciazine sulfoxide. A highly sensitive, simple and rapid method for determination of the Periciazine by fluorescence of its oxidation product with Oxone ® solution in 0.02 M hydrochloric acid solution (λex = 364 nm; λem = 444 nm) has been developed. The calibration plot is linear in concentration range of 0.05 – 4.00 µg mL -1 . LOQ (10S) is 0.05 µg mL -1 . The possibility of quantitative determination of Periciazine in pharmaceutical preparations (Neuleptil ®, 10 mg capsules and Neuleptil ®, a 30 mL 4 % oral (solution) drops) has been shown RSD < 2.2 % (δ < RSD).","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67435405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation Of Carbon Paste Electrode Modified With Heavy Metals For The Analysis Of Paracetamol By Voltammetry And Impedance Spectroscopy 用伏安法和阻抗法评价重金属修饰碳糊电极对乙酰氨基酚的分析
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2020-01-01 DOI: 10.17721/moca.2020.93-99
Hayat E.L.Ouafy, T. E. Ouafy, M. Oubenali, M. Mbarki, M. Echajia
{"title":"Evaluation Of Carbon Paste Electrode Modified With Heavy Metals For The Analysis Of Paracetamol By Voltammetry And Impedance Spectroscopy","authors":"Hayat E.L.Ouafy, T. E. Ouafy, M. Oubenali, M. Mbarki, M. Echajia","doi":"10.17721/moca.2020.93-99","DOIUrl":"https://doi.org/10.17721/moca.2020.93-99","url":null,"abstract":"The present document reports on the electrocatalytic activity of heavy metals (Copper(II), Nickel(II), Manganese(II) and Lead(II)) modified carbon paste electrodes (HM/CPE) for the paracetamol (Pr) oxidation where its results are compared with carbon paste electrode. The voltammetric behavior of Pr is explored where a sensitive anodic peak has appeared at about 0.27 V (vs. Ag/AgCl/3 M KCl) in 0.1 M Na2SO4 (pH 12). This peak results from the irreversible oxidation of Pr at HM/CPE surface. The catalytic effect was evaluated using cyclic and square wave voltammetry. Electrochemical impedance spectroscopy also confirms our experimental results as the HM/CPE shows the least charge transfer resistance. Also, HM/ CPE can be utilized successfully to ameliorate the electroanalysis of Pr at very weak concentration with excellent sensitivity. The calibration curves were linear from 6.0 · 10-5 to 8.0 · 10-4 mol L-1. The detection limits were found approaching 10-9 mol L-1. Then, the proposed method was applied to detect Pr in river water samples with satisfactory results.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67435479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Sorption Preconcentration Of Silver For Atomic Absorption Analysis And Antibacterial Properties Of The Acid-Modified Clinoptilolite – Ag Composite 酸修饰斜沸石-银复合材料的银原子吸收吸附预富集及抗菌性能
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2020-01-01 DOI: 10.17721/moca.2020.73-82
V. Vasylechko, V. Fedorenko, O. Gromyko, G. Gryshchouk, Y. Kalychak, S. Tistechok, I. Us, A. Tupys
{"title":"Sorption Preconcentration Of Silver For Atomic Absorption Analysis And Antibacterial Properties Of The Acid-Modified Clinoptilolite – Ag Composite","authors":"V. Vasylechko, V. Fedorenko, O. Gromyko, G. Gryshchouk, Y. Kalychak, S. Tistechok, I. Us, A. Tupys","doi":"10.17721/moca.2020.73-82","DOIUrl":"https://doi.org/10.17721/moca.2020.73-82","url":null,"abstract":"Sorptive properties of the acid-modified Transcarpathian clinoptilolite towards Ag(I) were studied under dynamic conditions. It was found that the most effective acid-modifier is 1 M HNO3 solution. The sorption capacity value of H-clinoptilolite under the optimal conditions is 6.15 mg of Ag per 1 g of zeolite. The solid-phase extraction procedure with acid-modified clinoptilolite was used to pre-concentrate trace amounts of silver ions in aqueous solutions and then finally to determine them by the atomic absorption method. Due to the acceptable recoveries (≥ 98 %) and RSD values (2.3-4.7 %) for tap and lake water, the developed method can be successfully applied for the determination of trace amounts of silver ions in the presence of major components of water. It was established that the initial form of clinoptilolite has a better antibacterial effect against E. coli than the H-form. However, antagonistic activity against S. aureus was lower than against E. coli in both forms. The combination of different forms of clinoptilolite with Ag(I) increases their antibacterial activity.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67435744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Voltammetric Determination Of Pt(IV) Using 5-Hydroxyimino-4- Imino-1,3-Thiazolidine-2-One 5-羟基亚胺-4-亚胺-1,3-噻唑烷-2- 1伏安法测定铂(IV
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI: 10.17721/moca.2019.130-139
P. Rydchuk, O. Tymoshuk, L. Oleksiv, T. Chaban, V. Matiychuk
{"title":"Voltammetric Determination Of Pt(IV) Using 5-Hydroxyimino-4- Imino-1,3-Thiazolidine-2-One","authors":"P. Rydchuk, O. Tymoshuk, L. Oleksiv, T. Chaban, V. Matiychuk","doi":"10.17721/moca.2019.130-139","DOIUrl":"https://doi.org/10.17721/moca.2019.130-139","url":null,"abstract":"5-Hydroxyimino-4-imino-1,3-thiazolidine-2-one has been suggested as a new organic reagent for the voltammetric determination of platinum. The optimal conditions of Pt(IV) voltammetric determination using 5-hydroxyimino-4- imino-1,3-thiazolidine-2-one on the peak of catalytic hydrogen currents have been determined: the background electrolyte is 0.3 M NaCl, pH of the solution is 2.0, 50-fold concentration excess of organic reagent. The sensitive method of Pt(IV) voltammetric determination was developed (LOD = 4.1·10-9 М). The range of determinable contents covers two concentration orders. The selectivity of the developed methods have been studied towards concomitant metals ions. The accuracy of the developed method has been tested on model solutions using “added-found” method. The method has been approved during the analysis of a real sample, viz. the catalyst for oxidation of exhaust gases of the car “Chevrolet”.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67434243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Monitoring And Distribution Of Organochlorine Pesticides, Polychlorinated Biphenyls And Polycyclic Aromatic Hydrocarbons In Surface River Water And Suspended Particulate Matter 地表水和悬浮颗粒物中有机氯农药、多氯联苯和多环芳烃的监测与分布
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI: 10.17721/moca.2019.117-129
M. Milyukin, M. Gorban, M. M. Skrynnyk
{"title":"Monitoring And Distribution Of Organochlorine Pesticides, Polychlorinated Biphenyls And Polycyclic Aromatic Hydrocarbons In Surface River Water And Suspended Particulate Matter","authors":"M. Milyukin, M. Gorban, M. M. Skrynnyk","doi":"10.17721/moca.2019.117-129","DOIUrl":"https://doi.org/10.17721/moca.2019.117-129","url":null,"abstract":"The monitoring results of organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs) and polycyclic aromatic hydrocarbons (PAHs) in Ukranian and world surface river waters were summarized. Total concentrations of OCPs, PCBs and PAHs in surface waters of Ukrainian rivers (Dnieper and Dniester) were 1.4–17.1; 2.8–57.2; 7.5–378.6 ng/dm3 , respectively. These pollution levels are medium on a global scale. Higher concentrations were recorded in the surface water of China, India and other Asian countries. Distribution of these organic ecotoxicants between water and suspended particulate matter (dispersionphase distribution) was analyzed and it was shown that in the majority of river waters ≥50% of them are associated with suspended particles. By the case of River Dnieper antibate correlations between watersoluble parts of individual OCPs, PCBs and PAHs and coefficient of their hydrophobicity were recorded. These correlations were presented graphically. In addition, relevant correlation coefficients and criterions of probability were calculated. The clearest correlation was established for PCBs.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67434146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Visual Binary Testing Of Hydrogen Sulfide Dissolved In Return Underground Local-Water Of Oil And Gas Condensate Fields 油气凝析田回地下局部水中硫化氢溶解的视觉二元检测
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI: 10.17721/moca.2019.146-152
E. A. Reshetnyak, N. Nemets, O. Chernyshova, A. Panteleimonov, V. M. Ostrovskaya
{"title":"Visual Binary Testing Of Hydrogen Sulfide Dissolved In Return Underground Local-Water Of Oil And Gas Condensate Fields","authors":"E. A. Reshetnyak, N. Nemets, O. Chernyshova, A. Panteleimonov, V. M. Ostrovskaya","doi":"10.17721/moca.2019.146-152","DOIUrl":"https://doi.org/10.17721/moca.2019.146-152","url":null,"abstract":"In order to screen samples of return underground local-water of oil and gas condensate fields for the presence of dissolved hydrogen sulfide, it was proposed to use single comparison sample for visual binary testing of H2S. Two indicator reactions occurring in solutions were selected — the formation of a stabilized suspension of MnS and CdS. Suspensions prepared in the presence of borate buffer (pH 9.18) and gelatin (stabilizer) are stable during the twenty four hours; a difference in the turbidity of suspensions can be observed near the normalized concentration of hydrogen sulfide (сlim = 15 mg L-1). When establishing the concentration of H2S in the comparison samples, a statistical approach was applied; the estimated values of the threshold concentration of H2S (ccomp.) are less than the normalized level on the value which providing the risk of a false-negative test result not more than 5% - 11.6 mg L-1 for MnS and 13.4 mg L-1 for CdS. Preference was given to a test system based on a stabilized suspension of CdS, because for this reaction, a narrower interval of unreliability and the value of ccomp. is nearer to the value of the сlim. The correctness of the visual binary testing of H2S in the samples of the analyzed water was confirmed by spectrophotometric method with p-phenylenediamine.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67434775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Development And Evaluation Of The Suitability Of The Method For Determining The Content Of Egg Coccidiostatics Using Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry (UPLC-MS/MS) 超高效液相色谱-串联质谱(UPLC-MS/MS)测定鸡蛋抑球虫含量方法的建立及适用性评价
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI: 10.17721/MOCA.2019.43-51
O. Bayer, O. Kaminska, L. Shevchenko, V. Mykhalska, О.М. Stupak, O. V. Bondarets, Yu.V. Dobrozhan
{"title":"Development And Evaluation Of The Suitability Of The Method For Determining The Content Of Egg Coccidiostatics Using Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry (UPLC-MS/MS)","authors":"O. Bayer, O. Kaminska, L. Shevchenko, V. Mykhalska, О.М. Stupak, O. V. Bondarets, Yu.V. Dobrozhan","doi":"10.17721/MOCA.2019.43-51","DOIUrl":"https://doi.org/10.17721/MOCA.2019.43-51","url":null,"abstract":"The conducted studies assessed the suitability of the method of ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) and established the MS/MS detection parameters and determined its validation characteristics for the analysis of residual content of coccidiostatics in food eggs. It has been proved that this method is accurate, practical and universal, which is confirmed by the data of ССα for amprolium – 2.14 μg/kg, diclazuril – 2.37 μg/kg, monensin – 2.34 μg/kg, narazin – 2.28 μg/kg, semduramicin – 2.23 μg/kg, toltrazuril 2.5 – μg/kg, salinomycin – 3.32 μg/kg, halofuginone – 6.18 μg/kg, maduramycin – 13.06 μg/kg, decoquinate – 2.37 μg/kg, robenidine – 26.06 μg/kg, nicarbazine – 316.7 μg/kg, percentage of return is 92.4 – 111 %. The results obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directives.The developed method allows to detect residual amounts of about 12 coccidiostatics that are used in poultry for the prevention of coccidiosis.","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"1 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67435017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Development Of ELISA Kit For Detection Of Glyphosate-Resistant Genetically Modified Soybean 抗草甘膦转基因大豆酶联免疫吸附测定试剂盒的研制
IF 0.7
Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI: 10.17721/MOCA.2019.21-29
Y. Khomenko, L. Ishchenko, V. Ishchenko, S. Midyk, D. Y. Rybalchenko, V. Ushkalov, V. Spyrydonov
{"title":"Development Of ELISA Kit For Detection Of Glyphosate-Resistant Genetically Modified Soybean","authors":"Y. Khomenko, L. Ishchenko, V. Ishchenko, S. Midyk, D. Y. Rybalchenko, V. Ushkalov, V. Spyrydonov","doi":"10.17721/MOCA.2019.21-29","DOIUrl":"https://doi.org/10.17721/MOCA.2019.21-29","url":null,"abstract":"The goal of research was development of competitive ELISA kit for detection of genetically modified plants resistant to glyphosate. As the source for gene isolation we have used leaves of GM soybean RoundUp Ready, line 40-3-2 (Monsanto). Using polymerase chain reaction the gene encoding the enzyme 5-enolpiruvilshykimat-3-phosphate synthase (CP4 EPSPS) was isolated, which provides resistance to the herbicide glyphosate. Isolated gene has been cloned into plasmid vector pET24(a), creating genetic construct pET-CP4EPSPS. E.coli (BL 21(DE3)) has been transformed by genetic construct and over expressed after induction by 0.5 mM IPTG. Recombinant enzyme CP4 EPSPS with molecular weight 45 kD was purified as inclusion bodies and used as antigen for 96-well polystyrene plates immobilization. Monoclonal antibodies CP4 EPSPS mAb3 (Artron BioResearch Inc., Canada) was used for synthesis of horseradish peroxidase conjugate and receiving diagnostic reagent. We have shown that in the competitive ELISA we can detect at least 0.1% of GM plants resistant to glyphosate, on the example of soybean (line RR 40-3-2), moreover we can also detect rapeseed (line GT73) and sugar beet (line H7-1).","PeriodicalId":18626,"journal":{"name":"Methods and Objects of Chemical Analysis","volume":"25 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67435029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信