Development And Evaluation Of The Suitability Of The Method For Determining The Content Of Egg Coccidiostatics Using Ultra Performance Liquid Chromatography - Tandem Mass Spectrometry (UPLC-MS/MS)

IF 0.7 Q4 CHEMISTRY, ANALYTICAL

Methods and Objects of Chemical Analysis Pub Date : 2019-01-01 DOI:10.17721/MOCA.2019.43-51

O. Bayer, O. Kaminska, L. Shevchenko, V. Mykhalska, О.М. Stupak, O. V. Bondarets, Yu.V. Dobrozhan

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引用次数: 1

Abstract

The conducted studies assessed the suitability of the method of ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS) and established the MS/MS detection parameters and determined its validation characteristics for the analysis of residual content of coccidiostatics in food eggs. It has been proved that this method is accurate, practical and universal, which is confirmed by the data of ССα for amprolium – 2.14 μg/kg, diclazuril – 2.37 μg/kg, monensin – 2.34 μg/kg, narazin – 2.28 μg/kg, semduramicin – 2.23 μg/kg, toltrazuril 2.5 – μg/kg, salinomycin – 3.32 μg/kg, halofuginone – 6.18 μg/kg, maduramycin – 13.06 μg/kg, decoquinate – 2.37 μg/kg, robenidine – 26.06 μg/kg, nicarbazine – 316.7 μg/kg, percentage of return is 92.4 – 111 %. The results obtained for assessing the suitability, accuracy and reproducibility of the results meet the requirements of the European Directives.The developed method allows to detect residual amounts of about 12 coccidiostatics that are used in poultry for the prevention of coccidiosis.

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超高效液相色谱-串联质谱(UPLC-MS/MS)测定鸡蛋抑球虫含量方法的建立及适用性评价

本研究对超高效液相色谱-串联质谱法(UPLC-MS/MS)的适用性进行了评价，建立了MS/MS检测参数，并确定了其用于食品鸡蛋中抑球藻残留量分析的验证特性。通过ССα对氨铵2.14 μg/kg、双唑齐2.37 μg/kg、莫能菌素2.34 μg/kg、那嗪2.28 μg/kg、semduramicin 2.23 μg/kg、托曲祖利2.5 μg/kg、盐碱霉素3.32 μg/kg、halofuginone 6.18 μg/kg、马杜洛霉素13.06 μg/kg、脱喹酮2.37 μg/kg、罗比尼定26.06 μg/kg、肼316.7 μg/kg的测定结果证实了该方法的准确性、实用性和通用性，回收率为92.4 ~ 111%。评估结果的适用性、准确性和可重复性所获得的结果符合欧洲指令的要求。所开发的方法允许检测用于家禽预防球虫病的约12种防球虫剂的残留量。

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来源期刊

Methods and Objects of Chemical Analysis CHEMISTRY, ANALYTICAL-

CiteScore

1.00

自引率

14.30%

发文量

期刊介绍： The journal "Methods and objects of chemical analysis" is peer-review journal and publishes original articles of theoretical and experimental analysis on topical issues and bio-analytical chemistry, chemical and pharmaceutical analysis, as well as chemical metrology. Submitted works shall cover the results of completed studies and shall make scientific contributions to the relevant area of expertise. The journal publishes review articles, research articles and articles related to latest developments of analytical instrumentations.