MOJ proteomics & bioinformatics最新文献

{"title":"Study of oxidative stress and antioxidant status in ascitic patients with ovarian cancer in comparison to liver cirrhosis patients","authors":"K. Pasha, M. GopalReddy, B. Rameshkumar, Q. Ayesha, M. Srinivasulu, K. Suseela, G. Sharma, L. Alex","doi":"10.15406/MOJPB.2017.06.00186","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.06.00186","url":null,"abstract":"The pathological accumulation of abdominal fluid (ascites) is due to multiple causes often associated either with peritoneal and non-peritoneal diseases. In the process of ascites formation there is increased vascular permeability, decreased lymphatic resorption and gross change in the concentrations of functional proteins and metabolites. Liver cirrhosis is a chronic disorder in which ascites develop in advanced stages of the disease, where as in ovarian cancer, most of the cases develop ascites in the early stages after the onset of disease. Among malignant ascites, ovarian cancers are the most common primary tumors and sixth most common malignant neoplasm in women with highest mortality rate.1 Oxidative stress is caused by free radicals leading to oxidative destruction of poly unsaturated fatty acids constitutive of cellular membrane and give rise to lipid peroxides which are unstable and decompose to form reactive carbonyl compounds. Among them malondialdehyde (MDA) is the most abundant, and it readily combines with several functional groups on proteins and forms DNA-MDA adducts, which are highly mutagenic, cytotoxic, co-carcinogenic and acts as a tumor promoter.2–5 Oxidative stress induces cancer among the biological molecules and plays an important role in the pathogenesis of liver disease and other hepatic alterations.6 Intestinal permeability is increased in patients with cirrhosis and oxygen free radicals play an important role in gut epithelial damage. Hence measurement of MDA is widely used as an indicator of oxidative stress. The antioxidant enzyme superoxide dismutase (SOD) is widely distributed in the cells and present in higher levels in erythrocytes,7 and it plays an important role in scavenging and protects cells against superoxide radical by dismutation of the highly reactive superoxide anion to oxygen and less reactive oxygen species hydrogen peroxide.8 The low levels of SOD may be due to increased utilization of scavenger lipid peroxides. The imbalance between the free radicals and antioxidants results in oxidative stress. Ceruloplasmin is a copper binding protein which is increased in several carcinomas.","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79346354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"B2B predictive analytics: an untapped sector","authors":"R. Boire","doi":"10.15406/MOJPB.2017.06.00185","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.06.00185","url":null,"abstract":"","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89957590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on micro RNAs and its application in psoriasis","authors":"Harishch, er An, Aram, D. An","doi":"10.15406/mojpb.2017.06.00184","DOIUrl":"https://doi.org/10.15406/mojpb.2017.06.00184","url":null,"abstract":"Psoriasis is a chronic and multifactorial autoimmune disorder in skin. In case of Psoriasis there is an abnormal hyper proliferation in keratinocytes, arised due to the activation of T-cells which produces a rich amount of arachidonic acid which leads to generation of various pro inflammatory mediators like PGs, LTs, cytokines and adhesion molecules via MAPK/AP-1, EARK1/2 and protein kinase-C (PKCs) activation pathways.1 In order to treat Psoriasis there is a need for the incorporation of naturally occuring bioactives like, omega (ω)-3 fatty acids (i.e., EPA and DHA) in a dose dependent manner which results in the inhibition of various pro-inflammatory mediators. Metabolization of EPA and DHA leads to the dampening of inflammation and higher resolution of abnormalities in skin. In case of natural treatment the synthesis of ω-3 PUFA-derived lipid mediators, namely resolvins and protectins have been widely used alone or in combination with other drugs in the treatment. Despite of their meritorious visages, the use of these bioactives is associated with several hiccups like higher range of instability and vulnerable to degradation due to lipid per oxidation. This treatment is also resulted in poor and inconsistent bioavilability by oral and topical administration. The potential use of nanomedicines in the delivery of such bio-actives has gained wider attention owing to their promise in enhancing the characteristics of bioavailability, improved stability and better efficacy. Moreover, barriers in the effective delivery of ω-3 fatty acids and how nanomedicines can be fit in the scope of its therapeutic delivery in psoriasis have also been addressed but it still has a long way to go with multidisciplinary aspects like Pharmacogenomics.","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90387863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational analysis of HSP-60 protein with structural insights into chaperonin containing TCP-1 subunit 5 in Bos taurus","authors":"Ravinder Singh, Vikash Kumar, C. Rajesh, A. Gurao, Anurag Kulshrestha, Manika Sehgal, A. Kaushik, Priyanka Sharma, S. Mishra, R. Kataria","doi":"10.15406/MOJPB.2017.06.00183","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.06.00183","url":null,"abstract":"Heat shock protein 60 kDa (HSP60) is a crucial chaperone that guides appropriate folding of denatured proteins under heat stress conditions. HSP60 provides assistance in correct folding of a multitude of denatured proteins. The Group II eukaryotic chaperonin TCP-1 ring complex is (TRiC or CCT) important cytosolic chaperonin which plays important role in folding of essential subset of cytosolic proteins and is believed to be highly conserved among different eukaryotic species. In this study, an extensive in silico analysis has been performed ranging from sequence comparison among species to homology modeling of Bos taurus Cct5 protein and determination of Hsp60 interacting partners. The comparative analysis of the protein revealed certain significant variations in Bos taurus. The experimental protein structure for Cct5 in Bos taurus is unknown till date, therefore the putative protein structure was determined using homology modeling. The stereo chemical properties of protein were assessed by utilizing several scrutiny tools such as PROCHECK, VERIFY3D and RAMPAGE to ensure model accuracy. The mode of interaction between HSF1 and Cct5 protein was evaluated by molecular docking studies.","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80136552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An attempt to find changes of post-translational modifications on serum proteins between embryonic 15.5 fetuses and newborn rats","authors":"Lilong Wei, Zhigang Wang, Lisi Zhu, Youhe Gao","doi":"10.15406/mojpb.2017.06.00182","DOIUrl":"https://doi.org/10.15406/mojpb.2017.06.00182","url":null,"abstract":"Blood is one of the most complex body fluids and plays important functions such as metabolic nutrient transport, signal conduction and immuno-modulatory. Proteins, the most important component of plasma, are considered the main executors of the physiological function of blood [1]. Posttranslational modifications (PTMs) of proteins can regulate protein functions by changing the physicochemical properties [2]. For example, phosphorylation or dephosphorylation may turn the kinase cascades in signaling on or off [3], and is often associated with tumorigenesis. So far, more than 500 types of PTMs or biological and chemical modifications have been identified and documented in the PSI-MOD [4], RESID [5], UniProt [6] and UniMod [7] databases. These translational modifications may determine the diversity of protein functions [8]. Research on post-translational modifications of plasma proteins with different physiological states could help to better understand and evaluate the function of plasma.","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81204482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review on diagnostic proteomics of depression","authors":"N. Samad, Sadia Jabeen, Z. Javeed, Nafeesa Jamil, S. Bibi, Zaitoon Ghani, Uzma Amin","doi":"10.15406/MOJPB.2017.06.00187","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.06.00187","url":null,"abstract":"Depression is a common and severe mental disorder, which can cause a considerable degree of disability to the individual and society [1]. The molecular mechanisms of depression are poorly understood even though many studies have been attempted. Moreover, there are currently no distinctive biological markers to aid in the diagnosis and treatment of depression [2]. The diagnosis, evaluation of medication effects, and prediction of relapse are carried out completely by clinical judgments based on the phenomenology of the disease [1]. The first step requires identifying the molecular pathway for depression. Genomic approaches have attempted to find the underlying molecular pathways, but have not successfully identified the major genes for depression [3]. This may be because there are multiple genes working together or interacting with the environment in a very complex way [1-3].","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"688 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87309728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Maximum likelihood estimation of the evolutionary distance of complete genomes of mitochondrial DNA between human and 16 animals","authors":"Omar Esmaill Hamadd, I. Akyol, M. S. Ekinci, Emin Özköse","doi":"10.15406/MOJPB.2017.05.00180","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.05.00180","url":null,"abstract":"Mitochondrial DNA (mtDNA) is a masterpiece of polynucleotide intelligence provided as a double stranded circular DNA, to be the spirit and manager of molecular activities in eukaryotic cells. The nucleic DNA activity and regulation depend on the signals and the levels of the tRNA and rRNA which mtDNA produced in the cell. The foremost attention-grabbing issue, mtDNA has the ability to adapt with each individual cell by modifying the sequence by slightly the initiation and termination points, likewise, the direction of transcription 5’=>3’ or 3’=>5’.1 Mitochondria generate most of the cellular energy within the form of adenosine triphosphate ATP, regulate cellular oxidation-reduction state and integrate several of the signals for initiating necrobiosis. By means of retrograde signaling, mitochondrial communicate of these events to the nucleus and thus modulate nuclear organic phenomenon and cell cycle. In human, mitochondrial pathology leads to a massive array of pathologies, and many diseases result from various defects of mitochondrial biogenesis and maintenance, metabolism chain complexes or individual mitochondrial proteins.2","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"50 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86732416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Review on Application of Biomarkers in the Field of Bioinformatics & Nanotechnology for Individualized Cancer Treatment","authors":"Harishch, er An, Aram","doi":"10.15406/MOJPB.2017.05.00179","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.05.00179","url":null,"abstract":"In the Current era of post genomics, there is an exponential growth in the area of genomics and proteomics data and hence there was a major advancement in the field of understanding the molecular mechanisms of complex diseases in humans. There raised an increase in pace to develop new technologies to diagnose the molecular signatures of complex diseases to initiate the therapy with respect to the principle of personalized medicine and hence the new era of molecular medicine laid the path for detection of disease, diagnosis of complex disorders and performing treatment with respect to the molecular profile of each and every individual [1-4]. This revolution had laid the foundation for the availability and application of new and novel biomarkers to predict the behavior of disease using advanced technologies to perform rapid diagnosis and detection. The evolutions of modern therapies to target cells were based on the principles of biocomputing. In order to address the molecular profiling and diagnostics, a major challenge was raised in characterization by histologic lesions in complex disorders because those lesions are heterogeneous at the cellular and molecular levels. In cancerous tumors, malignant cells are intermixed with blood vessels, stroma, and inflammatory cells [5-8]. Current technologies like Gene microarrays and Real-Time Polymerase Chain Reactions (RT-PCR) were not designed to handle the heterogeneic nature of cancer lesions and hence the development of nanotechnology provided a new opportunity to integrate morphological profile of diseased contion and molecular signatures and it is also used for correlating the observed cellular and molecular changes with respect to the behavior of pathological condition in complex disorders [9-11]. In particular, the application of bio conjugated quantum dots (QDs) [12-15] quantifies the presence of multiple biomarkers in intact tissue specimens and cancer cells and it allows us to conduct a comparative test between traditional histopathology V.S. molecular signatures of the same tissue [16-20]. Nanotechnology is being used in the fields of molecular imaging and therapy and it can be also be used to improve the toxicity and efficacy profiles of chemotherapeutic agents because these agents can be covalently attached or encapsulated [21-23].","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76907949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clear cell carcinoma of the breast in a developing community: what if the kidney?","authors":"W. Onuigbo","doi":"10.15406/mojpb.2017.05.00178","DOIUrl":"https://doi.org/10.15406/mojpb.2017.05.00178","url":null,"abstract":"The author in 2003 published the book, “Primary Pathology.” Among the different diagnoses was the “clear cell type” that characteristically showed clear staining of the cytoplasm. Five cases of it were found among the Ibo/Igbos of South-eastern Nigeria on using the method of histopathology data pool that facilitates epidemiological analysis. These were then documented. Thereafter, it was theorized that, since the kidney was another seat of clear cell cancer, it was recognized as being researchable if the patients had been investigated from that direction! This side of the research will merit the bioinformatics approach when the opportunity arises.","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84307359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular docking and pharmacokinetic of highly specific novel pan-mtor inhibitors against solid tumors","authors":"M. Naveed, Safia Zia, M. Akhtar, Fatima Ashraf, A. Afroz","doi":"10.15406/MOJPB.2017.05.00177","DOIUrl":"https://doi.org/10.15406/MOJPB.2017.05.00177","url":null,"abstract":"","PeriodicalId":18585,"journal":{"name":"MOJ proteomics & bioinformatics","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2017-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82213596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}