Microbiology Indonesia最新文献_第5页

Antibacterial Activity Test of Indigenous Yeast from Sapodilla Fruit against Staphylococcus aureus and Escherichia coli 皂角果原生酵母对金黄色葡萄球菌和大肠杆菌的抑菌活性试验

Microbiology Indonesia Pub Date : 2020-01-27 DOI: 10.5454/mi.13.4.1

G. L. Utama, Mutiara Nabila, H. R. Arifin, E. Lembong, T. Rialita

{"title":"Antibacterial Activity Test of Indigenous Yeast from Sapodilla Fruit against Staphylococcus aureus and Escherichia coli","authors":"G. L. Utama, Mutiara Nabila, H. R. Arifin, E. Lembong, T. Rialita","doi":"10.5454/mi.13.4.1","DOIUrl":"https://doi.org/10.5454/mi.13.4.1","url":null,"abstract":"The research aimed to identify indigenous yeast antibacterial activity from sapodilla fruit against Escherichia coli and Staphylococcus aureus, which conducted by experimental methods and followed by descriptive analysis. This study was done by the isolation of indigenous yeast, macroscopic and microscopic identification, yeast identification using RapID Yeast Plus System, antibacterial test by measuring the clear zone diameter, testing of pathogenic bacteria viability against indigenous yeast and identification of organic acid produced by yeast. The results of yeast isolation obtained 1 isolate (Saccharomyces cereviseae 1) from fruit and 3 isolates form sapodilla skin (S.cereviseae 2, Candida famata, and Pichia anomala) which had antibacterial activity against E. coli and S. aureus except C. famata isolates. Isolates with the largest antibacterial activity against E. coli and S. aureus based on the clear zone diameter were S. cerevisiae (2) isolates. The results of organic acid analysis by HPLC found that S.cerevisiae (2) isolate produced the highest organic acid namely acetic acid as much as 2.442 mg mL -1.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82420259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Lactic Acid Bacteria from Tempeh and Their Ability to Acidify Soybeans in Tempeh Fermentation 豆豉中的乳酸菌及其在豆豉发酵中酸化大豆的能力

Microbiology Indonesia Pub Date : 2020-01-01 DOI: 10.5454/MI.14.4.4

Tati Barus, Gabriela Giovania, B. Lay

引用次数: 2

Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line 重组2型登革病毒非结构1蛋白在哺乳动物细胞系中的表达

Microbiology Indonesia Pub Date : 2019-12-27 DOI: 10.5454/MI.13.1.%P

I. Rusmana

{"title":"Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line","authors":"I. Rusmana","doi":"10.5454/MI.13.1.%P","DOIUrl":"https://doi.org/10.5454/MI.13.1.%P","url":null,"abstract":"Dengue infection is a global infectious disease with almost 100 million cases occur annually in over more than 100 endemic countries. Dengue virus (DENV), the causative agent of dengue infection, is an 11 kbp RNApositive strand virus which encode 3 structural and 7 non-structural protein within its genome. Non-structural 1 (NS1) protein of DENV is expressed in the earlier stage of infection and having pathogenic role in disease severity. NS1 gene of DENV serotype-2 Indonesian strain was amplified through PCR method using specific designated primers. NS1 amplicon were then cloned into pUMVC4.a and pcDNA3.1 mammalian expression vector which confirmed through colony PCR and sequencing method. Recombinant pUNS1 and pcNS1 plasmids were transfected into CHO-K1 mammalian cell line with lipid based method. Recombinant NS1 protein expression were analyzed through immunostaining using dengue patient sera and rapid NS1 detection kit. Recombinant pUNS1 and pcNS1 plasmids were successfully constructed and recombinant NS1 protein was expressed in CHOK1 mammalian cell line and shown to be reactive against dengue patient sera. Our recombinant NS1 protein also tend to be released outside the transfected CHO-K1 cells as detected in rapid NS1 detection kit. Recombinant dengue NS1 protein was expressed in mammalian cell line in both intra and extracellularly and shown to be immunogenic.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"72 1","pages":"36-42"},"PeriodicalIF":0.0,"publicationDate":"2019-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73292375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Growth Characteristics of Chikungunya Virus Isolate from Indonesia in Various Human Cell Lines in vitro 印度尼西亚基孔肯雅病毒分离株在多种体外人细胞系中的生长特性

Microbiology Indonesia Pub Date : 2019-08-15 DOI: 10.5454/mi.13.1.1

O. N. Turnip, Rahma F. Hayati, Rizka Alawiyah, B. Yohan, Dionisius Denis, A. Bowolaksono, A. Soebandrio, R. T. Sasmono

{"title":"Growth Characteristics of Chikungunya Virus Isolate from Indonesia in Various Human Cell Lines in vitro","authors":"O. N. Turnip, Rahma F. Hayati, Rizka Alawiyah, B. Yohan, Dionisius Denis, A. Bowolaksono, A. Soebandrio, R. T. Sasmono","doi":"10.5454/mi.13.1.1","DOIUrl":"https://doi.org/10.5454/mi.13.1.1","url":null,"abstract":"Chikungunya (CHIK) fever, a febrile illness caused by Chikungunya virus (CHIKV) infection, is one of mosquito-borne viral diseases affecting people living in the tropical and subtropical regions in the world. The pathogenesis of the disease is yet to be completely unraveled, and research on CHIK has been conducted by employing various methods, including using cell lines to investigate the biological characteristics of CHIKV in vitro. To assess the suitability of human cell line model for CHIK study, various human cell lines including A549, Huh7, and HepG2 were infected with CHIKV and assayed for their susceptibility to infection. The MTT and plaque assay methods were performed to measure cell viability and virus growth kinetics, respectively. Fluorescence-activated Cell Sorting (FACS) and immunofluorescence assay were performed to measure the proportion of infected cells in the system and their morphological visualization. Both A549 and Huh7 human cell lines showed stable high cell viability upon infection while CHIKV growth kinetics were significantly lower in these cells compared to Vero-CCL81, a monkey cell line that is routinely used in other arboviruses research. Interestingly, we observed significantly different results in HepG2 human cell line, in which cell viability and CHIKV growth kinetics were significantly higher. FACS and immunofluorescence assay confirm the higher infection rate of CHIKV in HepG2 than A549 human cell line. We concluded herethat human hepatocytes HepG2 cell line was susceptible to Asian Genotype of CHIKV and proposed as an alternative cell for the in vitro CHIKV studies to the commonly used A549 and Vero cells.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90828007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Isolation of a Functional Gene Encoding Homologous Lysophospholipase from Indonesian Indigenous Bacillus halodurans CM1 印尼本土嗜盐芽孢杆菌CM1同源溶血磷脂酶功能基因的分离

Microbiology Indonesia Pub Date : 2019-08-15 DOI: 10.5454/mi.13.1.2

S. Fernanda, A. Abinawanto, I. Helianti

{"title":"Isolation of a Functional Gene Encoding Homologous Lysophospholipase from Indonesian Indigenous Bacillus halodurans CM1","authors":"S. Fernanda, A. Abinawanto, I. Helianti","doi":"10.5454/mi.13.1.2","DOIUrl":"https://doi.org/10.5454/mi.13.1.2","url":null,"abstract":"Lipase is a biocatalyst widely used in industry, for example detergent, pharmaceutical, food, or oil purification. One of the most widely lipase used for oil purification is lysophospholipase. As much as 50% of industrial enzyme needs are supplied from microorganisms. However, enzyme productivity from wild type microbial strain is usually limited and not applicable in industry, so that genetic engineering is necessary. Cloning gene encoding for lysophospholipase from Aspergillus niger and Cryptococcus neoformans have been conducted, but has never been conducted from alkalothermophilic bacteria, such as Bacillus halodurans. Bacillus halodurans CM1 is an alkalothermophilic bacterial strain isolated previously that has many industrially potential enzymes. This study aimed to isolate one of the gene encoding lipase from Bacillus halodurans CM1 and cloned into Escherichia coli DH5α using the pGEM-T easy vector. The gene fragment encoding lysophospholipase obtained with size 783 base pairs and had 100% similarity with gene encoding lysophospholipase from Bacillus halodurans C-125 (No access GenBank: BA000004.3). E. coli harbouring the recombinant plasmid with the gene also showed activity on trybutiryn medium compared to negative control.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89157260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Endophytic Fungi in Paraserianthes falcataria: Production of Indole Acetic Acid 镰形副花内生真菌:吲哚乙酸的生产

Microbiology Indonesia Pub Date : 2019-08-15 DOI: 10.5454/mi.13.1.3

R. S. Wulandari, R. Suryantini

{"title":"Endophytic Fungi in Paraserianthes falcataria: Production of Indole Acetic Acid","authors":"R. S. Wulandari, R. Suryantini","doi":"10.5454/mi.13.1.3","DOIUrl":"https://doi.org/10.5454/mi.13.1.3","url":null,"abstract":"Identification of endophytic fungi in Paraserianthes falcatria is the effort of the potential of endophytic fungi as phytohormone producer. Phytohormone is needed to spur shoot and root initiation. This study in P. falcatariais necessary when woody of P. falcataria decreases every year. The aimed of the study were to identify endophytic fungi from leaves, twigs, and roots of P. falcataria, and determine IAA content from endophytic fungi. Isolates that were grown from leaves, twigs and roots cuttings on PDA, were identified based on micro- and macromorphology. Determining of IAA content was counted with spectrophotometer vis based on a calibration curve from the standard solution. The results were obtained 10 of isolates fungi from leaves, twigs, and roots. But from 10, only nine isolates that could be identified. They were Aspergillus sp., Acremonium sp., Cladosporium sp., Trichoderma sp. 1, Phytium sp., Rhizoctonia sp., Trichoderma sp. 2, Hormiscium sp. 1 and Hormiscium sp. 2. Production of indole acetic acid (IAA) from Cladosporium sp. had the highest content than others (311 ppm). The lowest IAA content (51.97 ppm) was produced by the Rhizoctonia sp. The study can be continued to find out their abilities as PGPF agents and biopesticides of P. falcataria seedlings.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83146491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Induced Defense Related Enzyme Activities of Tomato Plant by Indigenous Endophytic Bacteria and Challenged by Ralstonia Syzigii Subsp. Indonesiensis 本地内生细菌对番茄植株防御相关酶活性的诱导及syziralstonia亚种的攻毒Indonesiensis

Microbiology Indonesia Pub Date : 2019-08-15 DOI: 10.5454/MI.13.1.4

Y. Yanti, W. Warnita, R. Reflin

{"title":"Induced Defense Related Enzyme Activities of Tomato Plant by Indigenous Endophytic Bacteria and Challenged by Ralstonia Syzigii Subsp. Indonesiensis","authors":"Y. Yanti, W. Warnita, R. Reflin","doi":"10.5454/MI.13.1.4","DOIUrl":"https://doi.org/10.5454/MI.13.1.4","url":null,"abstract":"Our previous research had screened 9 best indigenous endophytic isolates for their ability to control Ralstonia syzigii subsp. indonesiensis, the causal agents of bacterial wilt disease in tomato ( Lycopersicon esculentum ) in green house condition. Those 9 strains were B acillus cereus EPL1.1.3 , B. cereus TLE2.3, B. toyonensis EPL1.1.4 , Serratia nematodiphila TLE1.1, B. anthracis SNE2.2, B. cereus E1.AB1.2, B. cereus E1AB2.1, Enterobacter cloacae subsp. dissolvens TLE2.2 and S. marcescens KLE3.3. The purposed of this study is to test the ability of the endophytic bacteria strains in increasing defense related enzyme activities of tomato. Bacterial strains were tested for its ability to induce the defense-related enzymes which were phenylalanine ammonia lyase (PAL), peroxidase (PO) and polyphenol oxidase (PPO) in roots and leaves of tomato plants. R. syzigii subsp. indonesiensis inoculated to host plants 7 days after the endophyte bacteria strains inoculation. Enzyme activities were recorded at 0, 1, 3, 5, 7, 9, 12 and 15 days after pathogen inoculation (dpi). It was observed that PAL, PO and PPO activities were significantly increased in all of the endophytic bacteria inoculated treatments compared to control plant. Activities of PAL in the leaves was fast similar to the roots; but PO activities was higher in the roots compared to that in the leaves, whereas PPO activities was higher in the leaves than in the roots. PAL and PO reached the maximum level at different time in the leaves (3 dpi and 15 dpi), in the roots (5 dpi and 12 dpi), whereas PPO in the leaves at 12 dpi and in the roots at 9 dpi.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"34 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81037547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro 重组2型登革病毒非结构1蛋白体外诱导外周血单核细胞TNF-α水平的研究

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.2.%p

V. Kurnianda, S. Faradilla, S. Karina, S. Agustina, M. Ulfah, C. Octavina, F. Syahliza, M. R. Ramadhan, S. Purnawan, M. Musman

{"title":"Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro","authors":"V. Kurnianda, S. Faradilla, S. Karina, S. Agustina, M. Ulfah, C. Octavina, F. Syahliza, M. R. Ramadhan, S. Purnawan, M. Musman","doi":"10.5454/mi.13.2.%p","DOIUrl":"https://doi.org/10.5454/mi.13.2.%p","url":null,"abstract":"Dengue infection is a global health problem with an increasing incidence every year and now endemic in more than 100 WHO countries. Dengue infection is caused by dengue virus (DENV) which is an RNA virus with positive single strand, with ±11kb genome size encoding 3 structural proteins, 7 non-structural proteins, and two Untranslated Region (UTR). NS1 protein is known to have a very important role in the development of severe DENV infection, by the direct effect causing host cells damage and indirect effect by activating immune response to induce the secretion of excess cytokines. Transfected CHO-K1 cells with recombinant pcNS1 plasmid was co-cultured with PBMC from healthy donor. 48 hours post co-cultured, cell supernatant was collected and TNF-α levels and NS1 recombinant were measured by ELISA. Recombinant NS1 protein was expressed in CHO-K1 mammalian cell line and able to induce TNF-α with higher levels compared to control. Recombinant NS1 protein expression was able to induce secretion of TNF-α with higher levels compared to control.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"55 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74035179","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant and Antibacterial Activities Enhancement of Solid-state Fermented Candlenut Kernels by Aspergillus oryzae 米曲霉增强固态发酵木糖仁的抗氧化和抗菌活性

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.2.2

Grace DOLOROSA LIMBONG, Levy NATHANAEL NABABAN, A. Manurung, Merry MERYAM MARTGRITA

{"title":"Antioxidant and Antibacterial Activities Enhancement of Solid-state Fermented Candlenut Kernels by Aspergillus oryzae","authors":"Grace DOLOROSA LIMBONG, Levy NATHANAEL NABABAN, A. Manurung, Merry MERYAM MARTGRITA","doi":"10.5454/mi.13.2.2","DOIUrl":"https://doi.org/10.5454/mi.13.2.2","url":null,"abstract":"According to several studies, solid state fermentation (SSF) can enhance antioxidant and antibacterial activity of natural sources, and microorganism that is widely used in this kind of research is Aspergillus oryzae . Therefore, this study employed SSF by A. oryzae to enhance antioxidant and antibacterial activity of candlenut kernel. Candlenut kernel powder, that has been moistened with 60% water, was inoculated with 10% (w/w) of 5-day-culture of A. oryzae, and was fermented for 9 days (until exponential phase; sample-1) and 12 days (until stationary phase; sample-2). The fermented candlenut kernels was extracted by ethanol and concentrated using rotary evaporator. Total phenolic content of control (unfermented extract), sample-1, and sample-2 are 0.183 mg -1 -1 -1 GAE g , 2.761 mg GAE g , and 4.194 mg GAE g , respectively. This results supported the IC value determined 50 -1 -1 -1 by DPPH (2,2-diphenyl-1-picrylhydrazyl) method, those are 617.11 μ g mL , 260.23 μ g mL , and 45.29 μ g mL . -1 These results revealed a very strong antioxidant activity (< 50 μ g mL ) in the sample fermented until stationary phase. Antibacterial assay against Staphylococcus aureus resulted diameter of inhibition zone 7.17 mm, 13.51 mm, and 18.51 mm, respectively; whereas against Pseudomonas aeruginosa resulted diameter of inhibition zone 6.52 mm, 11.786 mm, and 15.269 mm, respectively. From this result, SSF until stationary phase enhanced higher antioxidant and antibacterial activity compared the other treatments.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85066428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Utilization of ArbuscularMycorrhizal Fungi For Planting Agarwood (Aquilariaspp) Seedling In Open Land 丛枝菌根真菌在露地种植沉香苗中的应用

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/MI.13.3.%P

A. Muin, Fachrizal Fachrizal

{"title":"The Utilization of ArbuscularMycorrhizal Fungi For Planting Agarwood (Aquilariaspp) Seedling In Open Land","authors":"A. Muin, Fachrizal Fachrizal","doi":"10.5454/MI.13.3.%P","DOIUrl":"https://doi.org/10.5454/MI.13.3.%P","url":null,"abstract":"Agarwood is a type of semitoleran plant, so that for planting the seedlings should be grown under the shade. For planting in open land, it requires treatment in which one of them is using seedlings inoculated with arbuscularmycorrhizal fungi. The Aim of the research were: (1) to obtain information on agarwood growth that has been inoculated with fungi mycorrhizalarbuscular when planted in the open land, and (2) ability to grow between agarwood seedlings inoculated mycorrhizal that was planted in the shade and in the open area. Split Plot randomized block design was applied with treatments : the first plot consisting of plant had been inoculated with mycorrhizae and without mycorrhizal inoculation, and the sub plot was the types of shading that consists of : open land, paranet 60 % intensity and natural vegetation. To reduce variabilty of site topographical differences were separated as bloks. Variables measured were :plant height (cm), stem diameter (mm), number of leaves and survival percentage of plant. The results show that the height and diameter growth of seedlings innoculated with mycorrhizae were higher than non innoculated. The seedlings innoculated with mycorrhizal fungi were planted in the paranet shading grew better and significantly different compared to the vegetation shading. Seedlings innoculatedmycorrhizal that were planted in open land grew better and significantly different compared to vegetation shading. This study results indicate that planting agarwood in the open land can be done using seedlings inoculated arbuscularmycorrhizal fungi.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91257955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0