Microbiology Indonesia最新文献_第6页

Chemotactic Motility and Growth of Pseudomonas fluorescens Towards Glucose Concentration 荧光假单胞菌对葡萄糖浓度的趋化运动和生长

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.2.1

Endah Rosa, U. M. Batubara, S. Suparjo

{"title":"Chemotactic Motility and Growth of Pseudomonas fluorescens Towards Glucose Concentration","authors":"Endah Rosa, U. M. Batubara, S. Suparjo","doi":"10.5454/mi.13.2.1","DOIUrl":"https://doi.org/10.5454/mi.13.2.1","url":null,"abstract":"Pseudomonas fluorescens is plant growth promoting rhizobacteria (PGPR) often inoculated on plants as natural biocontrol agent capable of protecting the plants from soil-borne pathogens. Chemotactic motility allows populations of P. fluorescens to rapidly search for nutrients and is an important factor determining their competitive success to colonize plant root. Therefore, we investigated various glucose concentrations from 0% to 1% (w/v) to enhance chemotactic motility and growth of this rhizobia. Chemotactic motility was evaluated using swim plate assay and bacterial growth was measured using UV-Vis Spectrophotometer in LB and M9 medium. Glucose with low concentration (0.05%) showed to have optimum response in P. fluorescens chemotactic motility with colony diameter 38.3 mm in LB medium and 12.8 mm in M9 medium. Highest growth of P. fluorescens was 7 -1 seen in control condition of LB medium reaching a peak at 0.0246 OD (~±1.44x10 CFU mL ) while growth in 600 7 -1 M9 medium supplemented with 1% glucose was just slightly lower with 0.0227 OD (~±1.32x10 CFU mL ). 600 Glucose in high concentration showed to repress chemotactic motility and first growth phase of P. fluorescens in LB medium due to catabolite repression.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82438080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Levels of TNF-α in PBMC (Peripheral Blood Mononuclear Cells) Induced by Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in vitro 重组2型登革病毒非结构1蛋白体外诱导外周血单核细胞TNF-α水平的研究

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.2.4

Fithriyah Sjatha, Oktivia CHANDRA MUSTIKA, Beti Ernawati Dewi, Tjahjani MIRAWATI SUDIRO

引用次数: 0

Antimicrobial Bioactive Compounds of Snail Seromucoid as Biological Response Modifier Immunostimulator 蜗牛类血清粘液抗菌活性化合物作为生物反应调节剂免疫刺激剂的研究

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.2.3

Agnes Sri Harti, N. Puspawati, Rahajeng Putriningrum

{"title":"Antimicrobial Bioactive Compounds of Snail Seromucoid as Biological Response Modifier Immunostimulator","authors":"Agnes Sri Harti, N. Puspawati, Rahajeng Putriningrum","doi":"10.5454/mi.13.2.3","DOIUrl":"https://doi.org/10.5454/mi.13.2.3","url":null,"abstract":"Anti-microbial bioactive compounds from snail ( Achatina fullica Ferussac) contained in snail seromucoid. It contains bioactive compounds such as glycans, peptides, glycopeptides, and chondroitin sulfate which can function as biological response modifiers (BRM) immunostimulators. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, and macrophages and releasing interferons and interleukin. Immunostimulators are compounds that can increase cellular immune responses in various ways, namely increasing the number and activity of T cells, NK cells, macrophages and releasing interferons and interleukins.The purpose of this study was to analyze antimicrobial bioactive seromucoid compound of snail ( Achatina fullica Ferrusac) as biological response modifiers (BRM) immunostimulators. The research methods based on experimental laboratory results with research stages including snail seromucoid isolation; antimicrobial activity; characterization physicochemical and profile of snail seromucoid proteins. The results of antimicrobial activity showed that 100% seromucoid concentrations had MIC (Minimal Inhibition Concentration) in Staphylococcus aureus, Candida albicans, and Pseudomonas aeruginosa . The physicochemical examination results showed specific gravity of 1.010; pH 8, -1 -1 -1 glucose 16 mg dL ; 9 mg dL cholesterol; protein 2.8 mg dL and negative heavy metals (Pb, Cu, Hg, Al). The results of the analysis of protein profiles showed that there were 3 subunits of proteins, range from 55 to 72 kDa and 1 specific protein sub unit of 43 kDa which was thought to be antimicrobial and biological response modifiers (BRM) immunostimulators.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86122176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Expression of Recombinant Non Structural 1 Protein of Dengue Virus Serotype-2 in Mammalian Cell Line 重组2型登革病毒非结构1蛋白在哺乳动物细胞系中的表达

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/mi.13.1.5

Fithriyah Sjatha, Oktivia CHANDRA MUSTIKA, Angky Budianti, Tjahjani MIRAWATI SUDIRO

引用次数: 1

The Antifungal Activity of Artesunate toward Candida albicans: Two Opposite Activities 青蒿琥酯对白色念珠菌的抗真菌活性:两种相反的活性

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/MI.13.3.5

Mohammad Rizki Fadhil Pratama, D. H. Tjahjono, T. Gusdinar

引用次数: 0

Antibacterial Potential of Radish Extract (Raphanus sativus L.) Against Fish Spoilage Bacteria 萝卜提取物的抑菌潜力防止鱼类腐坏细菌

Microbiology Indonesia Pub Date : 2019-01-01 DOI: 10.5454/MI.13.3.3

Eveline Eveline, Chikita Wini Tanumihardja

{"title":"Antibacterial Potential of Radish Extract (Raphanus sativus L.) Against Fish Spoilage Bacteria","authors":"Eveline Eveline, Chikita Wini Tanumihardja","doi":"10.5454/MI.13.3.3","DOIUrl":"https://doi.org/10.5454/MI.13.3.3","url":null,"abstract":"Radish ( Raphanus sativus L.) root is commonly used as flavor enhancing additive or side dish. Previous research revealed the presence of active compound in which could inhibit bacterial growth. Thus, a research concerning natural antibacterial for fish products that are categorized as high-risk food being contaminated by spoilage bacteria ( Pseudomonas aeruginosa, Bacillus cereus, dan Staphylococcus aureus ) was done. Radish root extraction was made by using ethyl acetate (semi polar) for 3 days. Well diffusion was performed using 4 extract concentration (10, 20, 30, dan 40% (w/v)) againts three fish spoilage bacteria. Based on our results, 30% concentration was the best concentration which inhibit more than 10 mm in inhibition zone with MIC and MBC. The scores were of 0.06% and 0.24% ( P. aeruginosa ), 0.13% and 0.50% ( S. aureus ), and 0.12% and 0.48% ( B. cereus ). Moreover, based on stability test against heating temperature showed that this extract concentration was more stable in 80°C with duration times for 5 minutes and pH 3 which resulting the lowest inhibition zone reduction compares to control extract. Radish extract was categorized as low toxic compound (LC 50 = 839.52 ppm) functioning in antibacterial compound containing major antibacterial compound ( bis(2-ethylhexyl) phthalate , 1,2- benzenedicarboxylic acid , 9,12,15-octadecatrienoic acid ), fatty acid ( n-hexadecanoic acid , butanedioic acid ), carboxylic acid ( isobutyric acid , malic acid , oleic acid ), and minor antibacterial compound ( n-Hydroxymethylacetamide , 2,4-bis(1,1-dimethylethyl) , 2,4-pentanedione , 2-Cyclohexen-1-one , hydrazine , cyclohexene oxide , gamma-sitosterol ).","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89737712","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover and editor information 封面和编辑信息

Microbiology Indonesia Pub Date : 2018-09-17 DOI: 10.5454/mi.12.1.%p

I. Helianti

引用次数: 0

Probiotic Potential of Lactic Acid Bacteria from Yellow Bamboo Shoot Fermentation using 2.5% and 5% Brine at Room Temperature 室温下2.5%和5%盐水发酵黄笋乳酸菌的益生菌潜力

Microbiology Indonesia Pub Date : 2018-08-12 DOI: 10.5454/mi.12.1.5

L. Lindayani, Laksmi Hartajanie, Monika Palupi Murniati

引用次数: 4

Detection of Antibody to Burkholderia pseudomallei in Captive and Wild Macaques 圈养猕猴与野生猕猴假玛利氏伯克氏菌抗体的检测

Microbiology Indonesia Pub Date : 2018-08-08 DOI: 10.5454/MI.1.1.%P

V. A. Testamenti, D. Iskandriati, A. Wahyudi, J. Pamungkas

引用次数: 2

Detection of Antibody to Burkholderia pseudomallei in Captive and Wild Macaquesin West Java and Bali, Indonesia 印度尼西亚西爪哇和巴厘岛圈养猕猴和野生猕猴假马尔样伯克氏菌抗体的检测

Microbiology Indonesia Pub Date : 2018-08-08 DOI: 10.5454/MI.12.1.4

V. A. Testamenti, D. Iskandriati, A. Wahyudi, J. Pamungkas

{"title":"Detection of Antibody to Burkholderia pseudomallei in Captive and Wild Macaquesin West Java and Bali, Indonesia","authors":"V. A. Testamenti, D. Iskandriati, A. Wahyudi, J. Pamungkas","doi":"10.5454/MI.12.1.4","DOIUrl":"https://doi.org/10.5454/MI.12.1.4","url":null,"abstract":"Melioidosis is an emerging zoonotic disease caused by the saprophytic Burkholderia pseudomallei, which infects human and a wide range of animal species. Melioidosis may lead to septicemia and pneumonia in human patients, which can be fatal if the patient is not treated accordingly. The disease is spread in tropical areas and is highly endemic to Southeast Asia and Northern Australia. However, melioidosis is poorly reported in Indonesia, especially in the veterinary field. This research provides serological evidence of antibodies to B. pseudomallei in both captive and wild nonhuman primates. Plasma samples were taken from a total of 390 monkeys in captivities and wild habitats in West Java and Bali, Indonesia. Enzyme-linked immunosorbent assay (ELISA) showed that the seroprevalence was 42.21% for Macaca fascicularis and 43.59% for Macaca nemestrina. Furthermore, the seroprevalence was 53.41% for captive macaques and 17.83% for wild macaques. The findings showed that exposure to B. pseudomallei happened in both captive and wild macaques. Based on this serosurveillance results, further studies such as comprehensive culture and clinical study are required to discover the clinical burden of the disease in nonhuman primates.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":"8 1","pages":"4-4"},"PeriodicalIF":0.0,"publicationDate":"2018-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75435929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3