Microbiology Indonesia最新文献_第10页

Application of Response Surface Method in Optimization of Medium Composition for Xylanase Production by Bacillus halodurans CM1 in Submerged Fermentation 响应面法在嗜盐芽孢杆菌CM1深层发酵产木聚糖酶培养基组成优化中的应用

Microbiology Indonesia Pub Date : 2016-09-30 DOI: 10.5454/mi.10.3.5

Sara Gustia Wibowo, I. Helianti, A. Suryani, Budiasih Wahyuntari

引用次数: 2

Molecular Identification of Endospore-Forming Rhizobacteria from Organic Cabbage Farm Potential as Biocontrol against Phytopathogen Xanthomonas campestris 有机白菜场内生芽孢根杆菌的分子鉴定及其防治油菜黄单胞菌的潜力

Microbiology Indonesia Pub Date : 2016-09-30 DOI: 10.5454/MI.10.3.4

Maya Fitriana Ilul Fahmi, E. Kusdiyantini, A. Suprihadi, D. Wulandari, A. Budiharjo

引用次数: 0

Diversity of Culturable Bacterial in Various Parts of Luwak's (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract 猕猴胃肠道不同部位可培养细菌的多样性

Microbiology Indonesia Pub Date : 2016-08-15 DOI: 10.5454/MI.10.2.4

S. Suhandono, Heri Setiadi, T. Kristianti, A. B. Kusuma, Andini, Warih Wedaringtyas, Demi T. Djajadi, I. Aryantha

引用次数: 8

Nitrogen Fixing Potential of Endophytic Bacteria Isolated from Aloe barbadensis Miller and Aloe sp. 芦荟和芦荟内生细菌的固氮潜能研究。

Microbiology Indonesia Pub Date : 2016-08-15 DOI: 10.5454/MI.10.2.2

R. F. W. Putrie, T. Widowati, S. Lekatompessy, H. Sukiman

{"title":"Nitrogen Fixing Potential of Endophytic Bacteria Isolated from Aloe barbadensis Miller and Aloe sp.","authors":"R. F. W. Putrie, T. Widowati, S. Lekatompessy, H. Sukiman","doi":"10.5454/MI.10.2.2","DOIUrl":"https://doi.org/10.5454/MI.10.2.2","url":null,"abstract":"Aloe is a crassulacean acid metabolism (CAM) species that are known to live in extreme enviroment such as drought condition. Nitrogen fixation procces influenced by the ability of plants to adapt in drought condition. Endophytic bacteria from Aloe and their ability for nitrogen fixation were little reported, but potential and its relationship between the ability for nitrogen fixing with resistance to drought conditions have not been reported. This research aimed study the endophytic bacteria from two varieties of aloe, namely Aloe barbadensis Miller and Aloe sp. in their ability on conducting the nitrogen fixing process and its relationship with resistance to drought. Characterization of endophytic bacteria were carried out by morphological observation of colony, Gram staining and molecular identification. Screening of nitrogen fixation was done using nitrogen-free semisolid NFb malate medium. Endophytic bacteria from Aloe sp. more than A. barbadensis in their potency of nitrogen fixation which related with habitat where their planted. A total of 40% of the endophytic bacteria isolates from the leaves of the aloe var. A. barbadensis and 62.5% of isolates from var. Aloe sp. are known to have a better ability to fixing nitrogen than the others. Isolates A barbadensis AB 12 and Aloe sp. AS 8 were the best isolates from each varieties on ability for nitrogen fixation. Based on 16S rRNA gene analysis those two selected isolates belonged to Bacillus methalotropicus strain DA 16-5 and Bacillus aryabhattai strain B8W22.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82611108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Antimicrobial Activity of Lactic Acid Bacteria from Bamboo Shoot Pickles Fermented at 15 oC 15℃发酵笋腌菜乳酸菌抑菌活性研究

Microbiology Indonesia Pub Date : 2016-08-15 DOI: 10.5454/MI.10.2.5

Laksmi Hartayanie, L. Lindayani, Monika Palupi Murniati

引用次数: 2

Cloning, Expression, and Functional Characterization of Autoactivated Human Prethrombin-2 Synthetic Gene by Using Pichia pastoris SMD1168 As a Host 以毕赤酵母SMD1168为宿主合成人凝血酶前2基因的克隆、表达及功能表征

Microbiology Indonesia Pub Date : 2016-07-26 DOI: 10.5454/MI.10.2.1

T. Subroto, Wulan Pertiwi, Muhammad Fadhillah, Khomaini Hasan, Ogi Budiantoro, S. Enus, S. Soemitro

{"title":"Cloning, Expression, and Functional Characterization of Autoactivated Human Prethrombin-2 Synthetic Gene by Using Pichia pastoris SMD1168 As a Host","authors":"T. Subroto, Wulan Pertiwi, Muhammad Fadhillah, Khomaini Hasan, Ogi Budiantoro, S. Enus, S. Soemitro","doi":"10.5454/MI.10.2.1","DOIUrl":"https://doi.org/10.5454/MI.10.2.1","url":null,"abstract":"Prethrombin-2 is a thrombin precursor that has important role in blood coagulation. It is the smallest precursor which is activated into thrombin by FXa prior to coagulation process. However, as a commercial theurapetic protein in fibrin sealant component, prethrombin-2 must be activated by ecarin before used. Thus, the production process of this protein needs further purification. In order to eliminate ecarin activation step and to increase production efficiency, we designed, cloned and expressed the recombinant autoactivated human prethrombin-2 in Pichia pastoris SMD1168. The variant was designed with 4 mutations, E40A, D47A, G48P, and E52A, following the result of a previous study. The synthetic variant gene was first optimized to conform with P. pastoris codon preference. The optimized synthetic gene was cloned in pD912 plasmid using X ho I and S ac II restriction enzymes. The transformed P. pastoris was selected on agar plate supplemented with 1,000 µg.mL -1 Zeocin as a selection marker. This study showed that autoactivated prethrombin-2 was succesfully expressed extracellularly by P. pastoris SMD1168. The activity of recombinant autoactivated prethrombin-2 using a chromogenic substrate S-2238 was 0.540 unit/mg. Taken together, these results demonstrated that autoactivated human prethrombin-2 was successfully produced extracellularly in P. pastoris .","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82598095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Comparison of Microbial Pattern Causing Urinary Tract Infection in Female Out- and Hospitalized Patients in Jakarta 雅加达市女性门诊与住院患者尿路感染微生物类型比较

Microbiology Indonesia Pub Date : 2016-07-26 DOI: 10.5454/MI.10.1.5

Yeva Rosana, D. Ocviyanti, A. Karuniawati, S. R. Akhmad

{"title":"Comparison of Microbial Pattern Causing Urinary Tract Infection in Female Out- and Hospitalized Patients in Jakarta","authors":"Yeva Rosana, D. Ocviyanti, A. Karuniawati, S. R. Akhmad","doi":"10.5454/MI.10.1.5","DOIUrl":"https://doi.org/10.5454/MI.10.1.5","url":null,"abstract":"Urinary Tract Infection (UTI) is an infection in any part of the urinary system. Women are 3 times more likely to have UTI than men. The UTI accounts for 15% infection cases in outpatients and 24% cases in hospitalized patients. Although the most common cause of UTI is certain bacteria, but it was not easy to choose the appropriate antimicrobial therapy. Strategy for choosing empiric antimicrobial treatments for UTI in female out- and hospitalized patients should be based on the pattern of the causative organisms. The aim of this study was to understand the microbial pattern causing UTI in female out- and hospitalized patients in Jakarta. The UTI -1 causative microorganisms were obtained from urine culture containing 100,000 cfu/mL . Twenty nine microorganisms were found as the causative agents of UTI in 317 pregnant women who came to six Community Health Centres (Puskesmas) in Jakarta: Makassar; Pulogadung, Cakung, Pasar Rebo, Duren Sawit, and Kramat Jati for antenatal care. Twenty nine microorganisms were isolated from 114 urine samples of female hospitalized patients who were diagnosed of UTI. The samples were obtained from the Microbiology Laboratory Clinic of FKUI-RSCM. The most common microorganisms causing UTI in female out- and hospitalized patients were Gram negative bacteria. In female outpatients, Klebsiella sp was the most common causative bacteria (31%), followed by Escherichia coli (24.1%). In female hospitalized patients, Escherichia coli was the most common causative bacteria (30%), followed by Candida sp (24.1%) and Klebsiella pneumonia (6.8%). There was more variation in the pattern of UTI causative organisms in hospitalized female patients in comparison to that of the outpatients. Candida sp. was only found in hospitalized UTI patients but not in outpatients.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86976651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Primary Tupaia javanica Hepatocytes Cultures As In Vitro Replication System for Ape Hepatitis B Viruses 原代土鼠肝细胞培养作为猿猴乙型肝炎病毒体外复制系统的研究

Microbiology Indonesia Pub Date : 2016-07-26 DOI: 10.5454/MI.10.2.3

M. Surya, D. Iskandriati, S. Mariya, U. Saepuloh, Permanawati Permanawati, D. Sajuthi, J. Pamungkas

{"title":"Primary Tupaia javanica Hepatocytes Cultures As In Vitro Replication System for Ape Hepatitis B Viruses","authors":"M. Surya, D. Iskandriati, S. Mariya, U. Saepuloh, Permanawati Permanawati, D. Sajuthi, J. Pamungkas","doi":"10.5454/MI.10.2.3","DOIUrl":"https://doi.org/10.5454/MI.10.2.3","url":null,"abstract":"Hepatitis B virus (HBV) is a DNA virus with liver as primary target organ.This virus caused chronic infection that can progress to cirrhosis, liver cancer and even death. In vitro model system of hepatocyte cultures is important and widely used to study a variety aspects of hepatitis B. Development of small animal Tupaia sp . for the in vitro model system is an alternative to the existinghepatocyte cultures. The specific purpose of the studyis to develop Tupaia javanica hepatocytes culture for HBV replication, and in a broader spectrum to answer the need for in vitro model of hepatocytes. Primary T. j avanica hepatocytes (PTH) culture was successfully maintained for 14 days to reach 80% confluence, and infection of Javan gibbon HBV (GiHBV) and orangutan HBV (OuHBV) onto the culture on day 15 showed viral replication for up to eight days as measured by polymerase chain reaction (PCR). PCR quantification indicated that the highest copy number of DNA virus was detected onday two anddecreased until day 8 after infection. Cell receptor for HBV attachment, known as sodium taurocholate cotransporting polypeptide was expressed on the surface of PTH and shown as green luminenscent when observed by immunofluorescence assay. Sequence of partialS gene from the apes HBVs after the viruses have been infected to the PTH showed amino acid identity to their wildtype as high as 99.29% for GiHBV and 95.71% for OuHBV. This study suggested that the PTH can support the replication of GiHBV and OuHBV.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90251457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterologous Expression of α-Amylase from Saccharomycopsis fibuligera R64 and its Tyr401Trp Mutant in Pichia pastoris 纤维酵母菌R64及其Tyr401Trp突变体α-淀粉酶在毕赤酵母中的异源表达

Microbiology Indonesia Pub Date : 2016-07-18 DOI: 10.5454/MI.10.1.4

Riezki Amalia, W. Ismaya, Fernita Puspasari, Khomaini Hasan, T. Subroto, D. Natalia, S. Soemitro

引用次数: 5

Utilization of the Bacteria Bacillus pumilus and Citrobacter youngae as Flotation Bioreagents in the Microflotation of Chalcopyrite, Pyrite, and Silica 细芽孢杆菌和幼柠檬酸杆菌作为浮选生物反应器在黄铜矿、黄铁矿和二氧化硅微浮选中的应用

Microbiology Indonesia Pub Date : 2016-06-16 DOI: 10.5454/MI.10.1.3

E. Sanwani, D. Hidayati, S. Chaerun

{"title":"Utilization of the Bacteria Bacillus pumilus and Citrobacter youngae as Flotation Bioreagents in the Microflotation of Chalcopyrite, Pyrite, and Silica","authors":"E. Sanwani, D. Hidayati, S. Chaerun","doi":"10.5454/MI.10.1.3","DOIUrl":"https://doi.org/10.5454/MI.10.1.3","url":null,"abstract":"Until presently, relatively toxic and expensive chemical reageants are routinely used in the flotation of sulfide and oxide minerals. To establish a more environmentally friendly flotation process, alternative flotation reagents have been explored extensively by using microbes and their metabolic products such as biosurfactants or EPS (extracellular polymeric substances) as high molecular weight biosurfactants. Hence, the present work focused on the application of the mixotrophic bacteria capable of both producing biosurfactants and oxidizing iron-sulfur (herein Bacillus pumilus strain SKC-2 and Citrobacter youngae strain SKC-4) as flotation bioreagents in the microflotation of chalcopyrite, pyrite and silica. Laboratory microflotation tests using both bacterial strains as bioreagents were evaluated as a function of conditioning time, pH and bacterial cell concentration. Experimental evidence indicated that the chalcopyrite recoveries could be achived using both bacterial strains but its better recovery was obtained with the bacterium Citrobacter youngae as bioreagents. The findings of this study thus suggest the possible application of these bacterial strains as flotation bioreagents in order for establishing a more eco-friendly mineral processing.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74417714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1