Microbiology Indonesia最新文献_第4页

Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated from Different Crop Soils 不同作物土壤中两种本地增磷细菌——污染伯克霍尔德菌PSB3和鲍曼不动杆菌PSB11的拮抗作用

Microbiology Indonesia Pub Date : 2020-08-18 DOI: 10.5454/mi.14.2.1

R. A. Nugroho, V. I. Meitiniarti, Chrisseptina Damayanti

{"title":"Antagonistic Effect of Two Indigenous Phosphate Solubilizing Bacteria, Burkholderia contaminans PSB3 and Acinetobacter baumannii PSB11 Isolated from Different Crop Soils","authors":"R. A. Nugroho, V. I. Meitiniarti, Chrisseptina Damayanti","doi":"10.5454/mi.14.2.1","DOIUrl":"https://doi.org/10.5454/mi.14.2.1","url":null,"abstract":"Phosphorus is the most important key element in the nutrition of plants. Although P is abundant in soils, it is a major limiting factor for plant growth as it is in an unavailable form for roots uptake. Phosphate solubilizing bacteria (PSB) has ability to convert insoluble form of P to an available form. This study was aimed at screening and characterizing phosphate-solubilizing bacteria from manure and different soils and to ascertain a potential benefit to use mixed cultures to improve P solubilization. A total of 12 PSB colonies were isolated on Pikovskaya's agar medium containing tricalcium phosphate. Out of 12 bacterial isolates, 2 isolates showed high phosphate solubilization index (2.17 and 1.83, respectively) were selected for further study. Based on the 16S rRNA gene sequence analysis, PSB3 was closely related to Burkholderia contaminans (99%), and PSB11 was closely related to Acinetobacter baumannii (99%). The mean P dissolved in liquid cultures of PSB3 and PSB11 in a 14-day -1 incubation were 96.7 and 39.3 mg l , respectively. Mixed inoculation of B. contaminans PSB3 and A. baumannii PSB11 could not increase the solubilization activity significantly, suggesting there is antagonistic behavior of one isolate towards another. As the interaction of these two isolates may be antagonistic, co-inoculation of these bacteria for P solubilization is not recommended. However, further study is needed to confirm these results.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85901553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Utilization of Leaves in Mine Reclamation Land as Organic Fertilizer with Effective Bioactivatory of Microorganism 4 (em4) and Molasses 利用矿区复垦地叶片作为有机肥有效活化微生物4 (em4)和糖蜜

Microbiology Indonesia Pub Date : 2020-08-18 DOI: 10.5454/mi.14.2.5

S. D. Nurtjahyani, Dwi Oktafitria, S. Sriwulan, N. Maulidina, I. Cintamulya, E. Purnomo

引用次数: 0

Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia 印尼Aceh Besar温泉中产纤维素酶的嗜热细菌的分离与鉴定

Microbiology Indonesia Pub Date : 2020-08-11 DOI: 10.5454/mi.14.1.4

Ruhul Khalila, L. Fitri, S. Suhartono

{"title":"Isolation and Characterization of Thermophilic Bacteria as Cellulolytic Enzyme Producer from the Hot Spring of Ie Seuum Aceh Besar, Indonesia","authors":"Ruhul Khalila, L. Fitri, S. Suhartono","doi":"10.5454/mi.14.1.4","DOIUrl":"https://doi.org/10.5454/mi.14.1.4","url":null,"abstract":"Cellulase enzymes can be isolated from thermophilic bacteria obtained from the hot spring Ie Seuum, Aceh Besar. This research aimed to recover and characterize the isolates morphologically and biochemically followed by determination of the thermophile bacterial isolates potential as cellulolytic enzyme producers, The sampling o o o method in this research was conducted by a purposive sampling at temperature of 70 C, 60 C, and 50 C. Isolation of thermophilic bacteria was carried out on nutrient agar (NA) media. There were four isolates of thermophilic o o o bacteria isolated recovered at 70 C, five isolates at 60 C, and seven isolates at 50 C. Of the 18 isolates obtained, 15 of them were able to produce cellulase enzymes. Cellulase enzyme production can be determined by the presence of clear zones around bacterial colonies on CMC media after addition of 1% congo red drops and wash with 1 M NaCl. The highest five Cellulolytic Index (CI) values w ere obtained from isolates ISB75; ISB64; ISB52; ISB54; and ISB56 that were 1.23; 2.22; 1.39; 1.59; and 1.10, respectively. Biochemical tests carried out on 5 isolates with the highest cellulolytic index values showed that the bacterial isolate were suspected to be from the genera of Bacillus sp.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88501893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104 利用自诱导型Plyb启动子及培养基优化在枯草芽孢杆菌DB104细胞密度依赖性表达重组木聚糖酶

Microbiology Indonesia Pub Date : 2020-07-01 DOI: 10.5454/mi.14.1.2

Haniyya Haniyya, Dini Achnafani, M. Ulfah, N. Nurhayati, I. Helianti

{"title":"The utilization of auto-inducible Plyb promoter and media optimation for cell density-dependent expression of recombinant xylanase in Bacillus subtilis DB104","authors":"Haniyya Haniyya, Dini Achnafani, M. Ulfah, N. Nurhayati, I. Helianti","doi":"10.5454/mi.14.1.2","DOIUrl":"https://doi.org/10.5454/mi.14.1.2","url":null,"abstract":"Strong promoters are one of the fundamental aspects to increase the level of gene expression, and one of approach to improve the recombinant enzyme productivity so that the efficiency of production cost for enzyme production in industrial scale can be reached. Here we assessed the application of a cell density-dependent promoter and media optimation to promote cell growth and protein expression of Bacillus subtilis without excess usage of inducers. An auto-inducible Pylb promoter that is potential to provide inducer-free enzyme production was cloned and introduced into xylanase recombinant system in B. subtilis DB104 by PCR cloning and protoplast transformation. A 200 bp target gene was successfully inserted in between xynCM1 ORF -coding for B. halodurans CM1 xylanase- and its native promoter sequence at the upstream region. The disruption of the native promoter was intended to replace the native promoter with Pylb. Recombinant xylanase gene under Pylb was successfully expressed in B. subtilis DB104 and the enzyme was produced at stationary phase. Different media with various concentrations of glucose and nitrogen were used to optimize recombinant xylanase expression. It achieved a higher level of xylanase expression compared to wild-type and recombinant xylanase with native promoter B. subtilis in media containing a 2-fold recipe of LB media thus leads to increase cell density and xylanase expression (81.461 U mL-1).","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74921313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial Potential of Star Anise (Illicium verum Hook. f.) Against Food Pathogen Bacteria 八角茴香的抑菌潜力。f。)预防食物致病菌

Microbiology Indonesia Pub Date : 2020-07-01 DOI: 10.5454/mi.14.1.3

Eveline Eveline, Agustin Novita

{"title":"Antibacterial Potential of Star Anise (Illicium verum Hook. f.) Against Food Pathogen Bacteria","authors":"Eveline Eveline, Agustin Novita","doi":"10.5454/mi.14.1.3","DOIUrl":"https://doi.org/10.5454/mi.14.1.3","url":null,"abstract":"Star anise (Illicium verum Hook. f.) is commonly used as spice and flavor enhancer in food. Previous research revealed the presence of active compound which could inhibit bacterial growth. Thus, in order to apply star anise as natural antibacterial agent in food product, a further research concerning antibacterial activity and stability of star anise was conducted. Crude extract of star anise was obtained using ethanol and acetone with maceration method for 3 days, then diluted to 10, 20, 30, 40, and 50% (w/v). Well diffusion was conducted against three food spoilage bacteria (Staphylococcus aureus, Escherichia coli, and Bacillus cereus). Extract from ethanol with 30% concentration was selected as the best extract in which inhibit more than 6 mm inhibition zone with MIC and MBC value: 1.59% and 6.36% (S. aureus), 1.04% and 4.18% (E. coli), and 0.59% and 2.39% (B. cereus). This selected extract was used to test the extract stability against 4 levels of heating temperature (60, 70, 80, and 90°C) for 2 levels of heating time (15 and 30 minutes), and 4 levels of pH (4, 5, 6, and 7). Based on our results, different heating treatment and pH caused extract instability. Star anise extract was more stable at 60°C for 15 minutes heating treatment and pH 4, which resulting the lowest inhibition zone reduction compared to control extract. Star anise extract was categorized as low toxic compound (LC50 = 212.09 ppm). Terpenoids (anethole, 2,6-dimethyl-6-(4-methyl-3-pentenyl)-2-norpinene, β-caryophyllene, β-bisabolene) was founded as major antibacterial compound in star anise extract; fatty acid (6-octadecenoic acid, hexadecanoic acid, stearic acid) and benzaldehyde (4-anisaldehyde, p-allylanisole) were also founded as minor compound.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85837134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Influence of indigenous mixotrophic bacteria on pyrite surface chemistry: Implications for bioflotation 本地混合营养细菌对黄铁矿表面化学的影响:对生物浮选的影响

Microbiology Indonesia Pub Date : 2020-07-01 DOI: 10.5454/mi.14.1.1

E. Sanwani, Nuslia Bayangkara Lamandhi, Halimatul Husni, S. Chaerun, W. Astuti, F. R. Mufakhir

{"title":"Influence of indigenous mixotrophic bacteria on pyrite surface chemistry: Implications for bioflotation","authors":"E. Sanwani, Nuslia Bayangkara Lamandhi, Halimatul Husni, S. Chaerun, W. Astuti, F. R. Mufakhir","doi":"10.5454/mi.14.1.1","DOIUrl":"https://doi.org/10.5454/mi.14.1.1","url":null,"abstract":"Given the low-cost and eco-friendly method, biotechnology has been widely utilized in industries as an alternative for physical and chemical processes, including in the biomining process (e.g., bioflotation and biobeneficiation). However, the use of biochemical reagent, which is selective for certain minerals, has not been well studied. This research was aimed to investigate the potential use of biosurfactant-producing mixotrophic bacteria as an alternative to chemical reagents during bioflotation and biobeneficiation process. Thirteen bacterial strains were investigated for their ability to produce biosurfactants and their effects on the surface properties of pyrite minerals. Bacteria-pyrite interaction experimental results showed that pyrite surface properties became more hydrophilic in the experimental systems inoculated with bacteria adapted with pyrite for 48 h than that without bacterial adaptation to pyrite, which was evidenced by the decrease in the contact angle of pyrite minerals by up to 50%. This evidence was also confirmed by the highest emulsifying index value (51.6%) attained during the bacteria-pyrite interaction. Hence, these bacteria can potentially be applied to selective flotation as pyrite depressants.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87043095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Effect of Aeration Rate on the Growth of Blue Green Microalgae by Aspergillus oryzae in Buffalo Dung as Alternative Media 曝气速率对米曲霉在牛粪中生长蓝绿微藻的影响

Microbiology Indonesia Pub Date : 2020-06-18 DOI: 10.5454/mi.13.4.4

Edwin Yonathan Gurning Gurning, Amos Imanuel, Nina Juliana Roberta Turnip, A. Manurung

{"title":"The Effect of Aeration Rate on the Growth of Blue Green Microalgae by Aspergillus oryzae in Buffalo Dung as Alternative Media","authors":"Edwin Yonathan Gurning Gurning, Amos Imanuel, Nina Juliana Roberta Turnip, A. Manurung","doi":"10.5454/mi.13.4.4","DOIUrl":"https://doi.org/10.5454/mi.13.4.4","url":null,"abstract":"The high demand of Arthrospiraplatensis as a veritable protein source encourages its mass production worldwide. Currently, mass production of Arthrospiraplatensis is hindered by the relatively high price of the growth media. Recently, it is discovered that Arthrospiraplatensis can be cultivated using buffalo dung as an alternative medium. Buffalo dung is an excellent source of nitrogen and phosphorus which are principal macronutrients for the growth of Arthospiraplatensis. In addition to nitrogen and phosphorus, carbon is also a macronutrient that is important to the growth of microalgae. The carbon source used by the microalgae is carbon dioxide, which is consumed through photosynthesis. Carbon dioxide can be derived directly from the atmosphere as atmospheric CO2 existing as much as 0.04%-v/v in air, which can be provided directly using an aeration pump into the growth medium microalgae. During the aeration process, CO2 mass transfer occurs from the gaseous phase into the liquid phase. This research aims to investigate the effect of the aeration rate on the growth of the blue-green microalgae Arthrospiraplatensisusing buffalo dung media as an alternative medium. Arthrospiraplatensis will be cultivated on buffalo dung media using various aeration rates to determine the effect of aeration on the specific growth rate (µ). The air will also be pumped into the growth medium without Arthrospiraplatensis at the specific aeration rates to determine the mass transfer coefficient (kLa) that occurs from the air leading to growth medium. Analysis of mass transfer coefficient (kLa) of carbon dioxide will be conducted using the sulfite method. Variation of aeration that used in this research are 0.2 vvm; 0.4 vvm; 0.6 vvm; 1.2 vvm; 2.4 vvm that has mass transfer coefficient dan specific growth rate are 0.005 min-1 and 0.1987 day-1; 0.009 min-1 and 0.2279 day-1; 0.012 min-1 and 0.2044 day-1; 0.034 min-1 and 0.1918 day-1; 0.035 min-1 and µ in 2.4 vvm can’t determine, respectively.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86186741","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Citric Acid Production from Toba Banana Peel (Musa acuminata Colla) through Submerged Fermentation using Aspergillus niger 黑曲霉深层发酵产柠檬酸的研究

Microbiology Indonesia Pub Date : 2020-05-14 DOI: 10.5454/mi.13.4.2

Meva Gustina E. Sidauruk, Surya Ningsih Hutauruk, M. M. Martgrita, A. Manurung

{"title":"Citric Acid Production from Toba Banana Peel (Musa acuminata Colla) through Submerged Fermentation using Aspergillus niger","authors":"Meva Gustina E. Sidauruk, Surya Ningsih Hutauruk, M. M. Martgrita, A. Manurung","doi":"10.5454/mi.13.4.2","DOIUrl":"https://doi.org/10.5454/mi.13.4.2","url":null,"abstract":"Toba banana peel waste is derived from Toba banana fruit (Musa acuminata Colla) processing. Local people utilized banana peel waste usually as livestock feed. The waste also can make an environmental problem if it is not handling well. Banana peel waste has a high content of carbohydrate that can be fermented to produce a more valuable product, one of which is citric acid. Citric acid is an organic acid that is consumed globally and produced in large quantities. In food and beverages industries, citric acid is used for various purposes due to its high solubility, non-toxic and good taste characteristics. The objective of this research is to determine the optimum conditions of submerged fermentation of banana peel to produce citric acid using Aspergillus niger. The treatments were various banana peel concentrations (5%, 10% and 15% w/v) added with 5% sucrose or 5% glucose (w/v). During the fermentation, pH was measured to determine pH changes indicated the production of citric acid. The results showed that the variation concentration of banana peel substrate and type of sugars affect citric acid production. The optimum condition of submerged fermentation by Aspergillus niger was obtained at 15% substrate concentration by adding 5% sucrose to produce 0.651% (w/v) of citric acid.","PeriodicalId":18546,"journal":{"name":"Microbiology Indonesia","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2020-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74872122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene Cloning of Xylanase Glycoside Hydrolase Family 11 from Bacillus halodurans CM1 in Escherichia coli DH5α 大肠杆菌DH5α中嗜盐芽孢杆菌CM1木聚糖酶糖苷水解酶家族11的基因克隆

Microbiology Indonesia Pub Date : 2020-05-14 DOI: 10.5454/mi.13.4.3

Muhamad Taufiqul Naufal, Agustin Krisna Wardani, I. Helianti

引用次数: 1

Prevalence of Hepatitis B Virus Infection in Blood Donors Based on Titer Hepatitis B Surface Antigen Examination (HBsAg) 基于乙型肝炎表面抗原(HBsAg)检测的献血者乙型肝炎病毒感染率

Microbiology Indonesia Pub Date : 2020-05-02 DOI: 10.5454/mi.13.4.5

S. D. Nurtjahyani, R. Handajani

引用次数: 0