Microvascular research最新文献

{"title":"Time-course and pressure-dependent changes in microvascular responses during ischemic preconditioning","authors":"Sean M. Lubiak ,&nbsp;Mason A. Howard ,&nbsp;Jeffrey T. Schmidt ,&nbsp;Nihar N. Patel ,&nbsp;Anuj J. Prajapati ,&nbsp;Niriham M. Shah ,&nbsp;Emma K. Herring ,&nbsp;David H. Fukuda ,&nbsp;Jeffrey R. Stout ,&nbsp;Joshua L. Keller ,&nbsp;Ethan C. Hill","doi":"10.1016/j.mvr.2025.104831","DOIUrl":"10.1016/j.mvr.2025.104831","url":null,"abstract":"<div><div>This investigation examined a moderate individualized pressure (i.e., percentage of total arterial occlusion pressure [TAOP]) compared to low and high absolute pressures on muscle tissue oxygenation (StO₂) throughout ischemic preconditioning (IPC). Fifteen males randomly completed three cycles of IPC at a low (20 mmHg [IPC_SHAM]), moderate (80% of TAOP [IPC_80%]), and high (220 mmHg [IPC_220mmHg]) pressure. Each cycle lasted 5 min at the assigned pressure, followed by 5 min of zero pressure on the dominant leg. StO₂ was measured continuously and StO₂ indices (i.e., downslope [StO_2down], minimum [StO_2min], upslope [StO_2up], maximum [StO_2max]) were analyzed with Bayesian models. StO_2down and StO_2min were pressure-dependent (IPC_SHAM &lt; IPC_80% &lt; IPC_220mmHg) as indicated by zero absent from all 95% high-density intervals (95% HDI) and 100% probability of an effect (Prob = 100%). During IPC_220mmHg, StO_2up increased from cycle 1 to cycles 2 and 3 but plateaued from cycle 2 to 3 as indicated by zero absent from all 95% HDI's and Prob ≥87.6%. Additionally, StO_2up was greater for IPC_80% and IPC_220mmHg relative to IPC_SHAM for cycles 1 and 2 but was pressure-dependent during cycle 3 as indicated by zero absent from all 95% HDI's and Prob = 98.8%. Lastly, StO_2max was greater for IPC_220mmHg than IPC_SHAM and IPC_80% as indicated by zero absent from all 95% HDI's and Prob = 100%. Collectively, using moderate individualized pressure elicited similar StO_2up as high absolute pressure, but only for cycles 1 and 2. These findings may be attributed to differences in the hypoxic-insult and/or vascular-related mechano-transduction stimuli.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104831"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144310193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-dimensional choroidal changes in diabetes and diabetic retinopathy: An ultrawide-field swept-source optical coherence tomography angiography study","authors":"Zhaoxia Zheng ,&nbsp;Lei Hu ,&nbsp;Yue Zhang ,&nbsp;Nianen Liu ,&nbsp;Xiaoya Gu ,&nbsp;Shuang Song ,&nbsp;Xiaobing Yu","doi":"10.1016/j.mvr.2025.104832","DOIUrl":"10.1016/j.mvr.2025.104832","url":null,"abstract":"<div><h3>Purpose</h3><div>To investigate choroidal changes in different stages of diabetic retinopathy (DR), and determine the effect of panretinal photocoagulation (PRP) on choroid based on volumetric measurements of ultrawide-field swept-source optical coherence tomography angiography (UWF-SS-OCTA).</div></div><div><h3>Methods</h3><div>This observational study included 56 healthy controls, 192 treatment-naïve DR patients, and 42 PRP-treated DR patients who have undergone UWF-SS-OCTA measurements. Treatment-naïve DR patients were further grouped according to varying severity of DR. Choroidal parameters were analyzed and compared among these groups according to central and peripheral areas. Spearman analysis was performed to determine the association between non-perfusion area (NPA) and choroidal parameters.</div></div><div><h3>Results</h3><div>Compared with healthy controls, choroidal thickness (CT) and volume (CV), including both vascular and stromal volume (CVV/a and CSV/a) were decreased in treatment-naïve DR patients in full range, while choroidal vascularity index (CVI) decreased only in the peripheral area (<em>P</em> = 0.04). In detail, choroid was thinning in no-DR (NDR) and mild NPDR, followed by an increased trend in moderate and late stages of DR. NPA was positively associated with CT, CV, CVV/a, and CSV/a in moderate and late stages of DR. Choroidal parameters decreased in PRP-treated eyes except for an increase of CVI in the central area.</div></div><div><h3>Conclusion</h3><div>Choroid was thinning in treatment-naïve DR eyes. Specifically, choroid decreased in the early stage and further increased with DR progression; increased expression of VEGF may be a key factor in choroidal thickening. PRP treatment could contribute to the redistribution of choroidal blood flow and improve the perfusion of the macula.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104832"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144330829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel in vivo porcine models of chronic ischemic tissue","authors":"Veronika Frelichova ,&nbsp;Robert Bem ,&nbsp;Jaroslav Chlupac ,&nbsp;Michal Dubsky ,&nbsp;Jitka Husakova ,&nbsp;Andrea Nemcova ,&nbsp;Ludek Voska ,&nbsp;Zuzana Simunkova ,&nbsp;Filip Tichanek ,&nbsp;Jiri Fronek","doi":"10.1016/j.mvr.2025.104845","DOIUrl":"10.1016/j.mvr.2025.104845","url":null,"abstract":"<div><div>There is a lack of reliable in vivo models that replicate limb-threatening ischemia in humans. To fill this gap, we developed and validated two novel porcine ischemic models: ischemic limb and dorsal flap models, both with and without streptozotocin-induced hyperglycemia (<em>N</em> = 3 per group, 12 in total). Hind limb ischemia model was induced via different arterial ligations, with two ischemic and three control wounds per animal. In the flap model, four full-thickness flaps were created on the dorsum with silicone sheets to block reperfusion, and excisional wounds were made on the top. One non-ischemic wound served as control. Transcutaneous oxygen pressure (TcPO₂), wound area, and microvascular density were measured, with TcPO₂ and wound area assessed longitudinally. Data analysis focused on detailed visualization and Bayesian hierarchical modelling to account for the small sample size. Developed models exhibited stable ischemia and prolonged wound healing, with TcPO₂ remaining under 30 mmHg over 28 days, and wound healing extending beyond two weeks. The flap model showed slower TcPO₂ recovery and greater chronicity compared to the limb model, without reliable effect of hyperglycemia. Thus, the porcine flap model shows the highest potential as a relevant model for chronic limb-threatening ischemia.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104845"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144682787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of neovascularization in murine osteoarthritis using micro-computed tomography","authors":"Reza Talaie,&nbsp;Pooya Torkian,&nbsp;Anthony Spano,&nbsp;Alexander Clayton,&nbsp;Jafar Golzarian","doi":"10.1016/j.mvr.2025.104844","DOIUrl":"10.1016/j.mvr.2025.104844","url":null,"abstract":"<div><h3>Purpose</h3><div>The study aimed to examine neovascularization in murine osteoarthritis (OA) using micro-computed tomography (μCT).</div></div><div><h3>Materials and methods</h3><div>OA was induced in eighteen mice through intra-articular collagenase injection, designating the left hindlimbs as OA models and the right hindlimbs as controls. Mice were monitored for 4, 8, or 12 weeks post-induction. Hindlimbs underwent overnight tissue fixation and were then subjected to μCT scanning. Quantification of unnamed arterial branches spanned from the femoral artery's terminal branching point to 2.5 mm below the tibial plateau.</div></div><div><h3>Results</h3><div>Baseline characteristics did not differ significantly between control and OA-induced groups (p &gt; 0.05). Collagenase-treated limbs showed a significantly higher number of unnamed arterial branches compared to controls (11.6 vs. 7.5, p &lt; 0.001), reflecting increased neovascularization. This elevation persisted across all post-induction time points, with no significant time-dependent trend (p = 0.09) or interaction between time and treatment group (p = 0.17). Spatial analysis revealed that neovessels were predominantly localized to peri-meniscal (61 %) and subchondral (29 %) regions.</div></div><div><h3>Conclusion</h3><div>Collagenase-induced OA in mice results in sustained and spatially patterned neovascularization, detectable using non-contrast μCT. These findings underscore the utility of μCT for tracking vascular remodeling in OA and highlight potential anatomical targets for angiogenesis-modulating therapies.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"161 ","pages":"Article 104844"},"PeriodicalIF":2.9,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144667978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperglycemia-induced blood-brain barrier dysfunction: Mechanisms and therapeutic interventions","authors":"Changsheng Chen ,&nbsp;Xi Xu ,&nbsp;Jiahao Lu ,&nbsp;Yuqing Xiang ,&nbsp;Linsheng Shi ,&nbsp;Dong Liu","doi":"10.1016/j.mvr.2025.104820","DOIUrl":"10.1016/j.mvr.2025.104820","url":null,"abstract":"<div><div>The blood-brain barrier (BBB) serves as a highly selective interface that regulates the transport of molecules between the blood and the brain. Its integrity is essential for maintaining neuronal homeostasis and preventing neuroinflammation. Hyperglycemia, a hallmark of diabetes, is linked to cognitive deficits and central nervous system (CNS) pathologies, including vascular dementia, stroke, and Alzheimer's disease, with BBB damage as a potential contributing factor. As the global prevalence of diabetes rises, understanding the connection between hyperglycemia and BBB dysfunction may facilitate the development of novel treatments that protect or restore BBB integrity, thereby alleviating the neurological complications of diabetes. Furthermore, it may aid in the development of targeted therapies for diabetes-related neurological complications. This literature review examines the emerging insights into the relationship between hyperglycemia and BBB dysfunction. It focuses on the mechanisms underlying BBB dysfunction, the clinical manifestations of this dysfunction in diabetes and cerebrovascular diseases, and potential therapeutic interventions.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104820"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144088816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activated partial thromboplastin time levels and coronary artery lesions in Kawasaki disease: A retrospective cohort study","authors":"Jinhui Zhou ,&nbsp;Chao Ni ,&nbsp;Zhenquan Wang ,&nbsp;Yuhan Xia ,&nbsp;Hongying Shi ,&nbsp;Xiaoshan Zhao ,&nbsp;Yufei Chen ,&nbsp;Chenchen Liu ,&nbsp;Xing Rong ,&nbsp;Rongzhou Wu ,&nbsp;Maoping Chu ,&nbsp;Huixian Qiu","doi":"10.1016/j.mvr.2025.104817","DOIUrl":"10.1016/j.mvr.2025.104817","url":null,"abstract":"<div><h3>Objective</h3><div>Kawasaki disease (KD) is an acute systemic inflammation, that affects medium-sized arteries. Coronary artery lesions (CALs) were the most serious complication or sequelae of KD. The intense inflammatory response leads to platelet activation, further exacerbating inflammation, which plays an important role in the pathogenesis of CALs in KD patients. Plus, coagulation factors are closely related to platelet activation. Therefore, we speculate that the activated partial thromboplastin time (APTT), an indicator of coagulation factor function, may be involved in the occurrence of CALs, but it has not been explored yet. This study aims to investigate the effect of the APTT level on CALs occurrence in the acute phase of KD.</div></div><div><h3>Methods</h3><div>A total of 2303 KD patients during a 10-year period were recruited at the Wenzhou Medical University affiliated Yuying Children's Hospital. A total of 1715 patients who completed the follow-up were enrolled in the final analysis and were divided into the low APTT group and the high APTT group at a 46 s cutoff before receiving intravenous immunoglobulin (IVIG) treatment. Multiple logistic regression analysis and stratified analysis were utilized to evaluate the independent impact of APTT levels on the occurrence of CALs and to determine the impact of APTT levels on the occurrence of CALs in different subgroups, respectively.</div></div><div><h3>Results</h3><div>The incidence of CALs in the low APTT group and the high APTT group was 12.5 % and 17.5 %, respectively (<em>P</em> = 0.005). Patients with high APTT levels had higher CRP levels (<em>P</em> &lt; 0.001). High APTT levels were the independent risk factor on the occurrence of CALs; the adjusted odds ratio (OR) was 1.523 (95 % CI: 1.144, 2.028). Similar results were found in stratification analysis and sensitivity analysis.</div></div><div><h3>Conclusions</h3><div>KD patients with high APTT levels (≥46 s) before IVIG treatment may be more prone to developing CALs in the acute phase of KD.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104817"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Day-to-day variability in cutaneous microcirculation measured with multi-exposure laser speckle contrast imaging and multispectral imaging","authors":"Moa Nilsson ,&nbsp;Martin Hultman ,&nbsp;Freya Richter ,&nbsp;Joakim Henricson ,&nbsp;Marcus Larsson ,&nbsp;Tomas Strömberg ,&nbsp;Ingemar Fredriksson ,&nbsp;Fredrik Iredahl","doi":"10.1016/j.mvr.2025.104819","DOIUrl":"10.1016/j.mvr.2025.104819","url":null,"abstract":"<div><h3>Introduction</h3><div>Dysfunctional microcirculation is associated with cardiovascular risk factors, chronic disease such as diabetes and acute conditions like septic shock. The non-invasive optical techniques laser Doppler flowmetry (LDF) and diffuse reflectance spectroscopy (DRS) are often used to measure perfusion and oxygen saturation, but are limited to single-point measurements making them sensitive to spatial variations. The imaging modalities multi-exposure laser speckle contrast imaging (MELSCI) and multi-spectral imaging (MSI) overcome this limitation by capturing the parameters in a larger skin area.</div></div><div><h3>Aim</h3><div>To assess the day-to-day variability of speed-resolved perfusion and oxygen saturation in the forearm and plantar foot at baseline and peak response following arterial occlusion-release, while also evaluating sex and age influences.</div></div><div><h3>Method</h3><div>MELSCI and MSI were used on 48 participants (12 males and 12 females aged 20–30, and 12 males and 12 females aged 50–60) across two measurements within a week. Each measurement lasted 60 min, with perfusion and oxygen saturation being measured at baseline (10 min), during occlusion (5 min), and post-occlusion (5 min) as spatial averages over the entire imaged tissue area.</div></div><div><h3>Results</h3><div>Older age was associated with higher foot perfusion at peak (<em>p</em> = 0.006). Variability (CV) ranged from 1.4 % to 19 %, with foot low-speed perfusion showing a sex- and age-related difference at peak (<em>p</em> = 0.007).</div></div><div><h3>Conclusion</h3><div>Age and sex influenced microcirculatory parameters, aligning with prior research. MELSCI and MSI demonstrated low day-to-day variability, making them promising techniques for clinical disease monitoring. The variability of MELSCI perfusion was lower than previously reported for laser speckle contrast imaging (LSCI) perfusion.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104819"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interplay between platelet and T lymphocyte after coronary artery bypass grafting (CABG): Evidence for platelet mediated post-CABG immunomodulation","authors":"Fateme Farhid ,&nbsp;Ehteramolsadat Hosseini ,&nbsp;Faranak Kargar ,&nbsp;Mehran Ghasemzadeh","doi":"10.1016/j.mvr.2025.104805","DOIUrl":"10.1016/j.mvr.2025.104805","url":null,"abstract":"<div><h3>Background</h3><div>On-pump coronary artery bypass grafting (CABG) triggers inflammatory responses as a result of surgical stress and extracorporeal circulation, which affect platelet and leukocyte activation while enhancing their intimate crosstalk. Given this, the study presented here aimed to investigate platelet-T cell interaction after CABG focusing on the changes in immunomodulatory subtypes of regulatory T Cells.</div></div><div><h3>Methods</h3><div>Blood samples were obtained from twenty patients undergoing on-pump CABG at 5 different time points of 24 h before, immediately, 2 h, 24 h, and one week after surgery. Total leukocyte and lymphocyte counts were determined using an automatic cell counter. Platelet P-selectin expression, frequencies of CD4⁺ and CD8⁺ T cells, platelet-T cell aggregates (PTCAs), and regulatory T cells derived from CD4⁺ (T4reg) and CD8⁺ (T8reg) cells, were assessed by flow cytometry.</div></div><div><h3>Results</h3><div>A significant increase in total leukocyte count occurred immediately after CABG, whereas, conversely, lymphocyte and CD4⁺ T cells but not CD8⁺ T cells decreased 2 h after surgery. However, all these changes returned to pre-CABG baseline levels within a week. Platelet P-selectin expression increased immediately after surgery, followed by a two-hour delay after PTCA, and both returned to baseline after one week. T4regs and T8regs showed a similar increase and decrease trend, where T8regs but not T4regs returned to baseline one week after surgery.</div></div><div><h3>Conclusion</h3><div>CABG surgery induces an inflammatory response that activates platelets and enhances P-selectin expression, facilitating PTCA formation. This mechanism is critical for the dynamics and differentiation of T cells, which play an essential role in post-CABG modulation of immune responses.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104805"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glial and blood-brain barrier cell-derived exosomes: Implications in stroke","authors":"Khiany Mathias ,&nbsp;Richard Simon Machado ,&nbsp;Taise Petronilho ,&nbsp;Victor Augusto Rodrigues Sulzbacher ,&nbsp;Victoria Linden de Rezende ,&nbsp;Josiane Somariva Prophiro ,&nbsp;Fabricia Petronilho","doi":"10.1016/j.mvr.2025.104812","DOIUrl":"10.1016/j.mvr.2025.104812","url":null,"abstract":"<div><div>Exosomes are small extracellular vesicles released by cells that play a pivotal role in intercellular communication, significantly influencing both the pathophysiology and potential treatment of ischemic stroke (IS). This review examines exosomes derived from key brain cell types, including microglia, astrocytes, oligodendrocytes, oligodendrocyte precursor cells (NG2+ cells), endothelial cells, and pericytes, emphasizing their molecular cargo and functional impact in IS. Microglia-derived exosomes regulate neuroinflammation, with M2-type exosomes exhibiting neuroprotective effects, while astrocyte-derived exosomes modulate pathways involved in pyroptosis and autophagy, influencing neuronal survival. Oligodendrocyte and NG2+ cell-derived exosomes contribute to remyelination, axonal growth, and inflammatory modulation. Endothelial and pericyte-derived exosomes play critical roles in BBB integrity, neurovascular remodeling, and drug transport across the BBB. This synthesis highlights recent advances in understanding how exosome-mediated communication impacts IS recovery and explores their translational potential for biomarker development and targeted therapies. By manipulating exosomal composition and delivery mechanisms, novel therapeutic strategies may emerge, offering hope for improved IS treatment outcomes.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104812"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143844264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proteomics suggests the role of Cxcl12 secreted by hucMSCs in the treatment of lipopolysaccharide-acute lung injury","authors":"Jinfeng Cui ,&nbsp;Liqing Luo ,&nbsp;Hongmei Geng ,&nbsp;Yunxiu Gao ,&nbsp;Yuanyuan Chen ,&nbsp;Qilin Yu ,&nbsp;Xiao Huang ,&nbsp;Xiaozhi Wang ,&nbsp;Ting Sun","doi":"10.1016/j.mvr.2025.104815","DOIUrl":"10.1016/j.mvr.2025.104815","url":null,"abstract":"<div><div>Acute respiratory distress syndrome (ARDS) is a clinical syndrome characterized by a high mortality rate, and its treatment is relatively straightforward. The application of human umbilical cord mesenchymal stem cells (hucMSCs) for the treatment of ARDS has emerged as a novel therapeutic approach and has been the subject of extensive research. In this study, a mouse model of acute lung injury (ALI) was established, and hucMSCs were administered via tail vein injection to investigate the pathogenesis of ARDS and the protein alterations following hucMSC treatment. Data-independent acquisition (DIA) was employed for the proteomic analysis of lung tissue, which included the identification of differentially expressed proteins (DEPs) and their associated pathways. The relevant DEPs identified in the lung tissues of the three groups of mice included Arid5a, Mrpl4, Cxcl12, and Rnf121 (<em>P</em> &lt;0.05). Silencing the expression of Cxcl12 in hucMSCs could significantly inhibit the therapeutic effect of hucMSCs in reducing the permeability of lung tissue and endothelial cells (<em>P</em> &lt; 0.05). Additionally, the signaling pathways associated with the relevant DEPs were analyzed. The DEPs and the enriched pathways discussed herein provide valuable insights into the pathogenesis of ARDS and the potential applications of hucMSCs.</div></div>","PeriodicalId":18534,"journal":{"name":"Microvascular research","volume":"160 ","pages":"Article 104815"},"PeriodicalIF":2.9,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143898991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}