Mikrobiyoloji bulteni最新文献

{"title":"[A Case Report of Cutaneous Scedosporium apiospermum Presenting with Cellulitis-like Cutaneous Findings and A Review of the Literature].","authors":"Elif Ayça Şahin, Zeynep Sena Solmaz, Kıvılcım Oğuzülgen, Mahmut Esat Tanribilir, Esra Adişen, Özlem Erdem, Hasan Selçuk Özger, Müge Aydoğdu, Gül Gürsel, Ayşe Kalkanci","doi":"10.5578/mb.20260150","DOIUrl":"10.5578/mb.20260150","url":null,"abstract":"<p><p>In this report a case of cutaneous Scedosporium apiospermum infection in 75-year-old woman with asthma who was receiving long-term therapy with corticosteroids was presented. She was hospitalized in the department of chest diseases for the follow-up of worsening dyspnea, cor pulmonale and stasis dermatitis. Erythema, localized tenderness and swelling developed on her left foot dorsum during her follow-up in the hospital. A few days later, black-colored papules with an erythematous base were observed on her foot dorsum and anterior surface of the tibia. She was diagnosed with cellulitis. Skin biopsy revealed numerous septated fungal hyphae. Preemptive amphotericin B was added to the antibacterial treatment. Within 48 h of incubation, the Scedosporium colony was isolated based on conventional methods. The fungal ribosomal RNA gene internal transcribed spacer sequence analysis revealed S.apiospermum and deposited into GenBank with accession number OM948685. The minimum inhibition concentration was defined as 1 µg/mL for amphotericin B; 16 µg/mL for fluconazole; 0.25 µg/mL voriconazole, 4 µg/mL for caspofungin, 1 µg/mL for itraconazole and 0.125 µg/mL for posaconazole on 48 h of incubation. The patient was transferred to the intensive care unit due to the deterioration of her general condition with the diagnosis of sepsis. Antimicrobial treatment was modified to caspofungin/ voriconazole combination and imipenem administration. Hepatic failure, bone marrow suppression with severe thrombocytopenia and disseminated intravascular coagulation syndrome developed on the third day of intensive care unit stay. The patient died of severe septic shock and multiorgan failure. We also reviewed the literature presenting similar cutaneous scedosporiosis cases and discussed the results.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"60 1","pages":"94-104"},"PeriodicalIF":0.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Changes in Gut Microbiome Profile in Pregnant Women with Hyperemesis Gravidarum: A Comparative Study Based on 16S rRNA Sequencing].","authors":"Özgür Koçak, Parisa Sharafi, Şeyma Gökdemir, Ümit Görkem, Ali Al-Kaleel, Ayşegül Taylan Özkan","doi":"10.5578/mb.2026018","DOIUrl":"10.5578/mb.2026018","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Hyperemesis gravidarum (HEG) is a pregnancy complication characterized by severe nausea, vomiting more than four times a day and dehydration, especially occurring in the first trimester of pregnancy. In recent years it has been shown that the intestinal bacterial microbiome profile may be associated with a wide range of diseases. The aim of this study was to determine whether the intestinal bacterial microbiome profiles differ between pregnant women diagnosed with hyperemesis gravidarum (HEG) and those with healthy pregnancies. Fresh stool samples were collected from 15 pregnant women diagnosed with HEG and 14 healthy pregnant women who did not have any complaints in the first trimester. After DNA isolation from the samples, 16S rRNA gene-based microbial profiling was performed with next-generation sequencing. The 16S rRNA V3-V4 region was sequenced with paired-end reads (2×250 base pair) on the Illumina MiSeq platform. The average sequence number for each sample was similar (HEG= ~2.54 million, control= ~1.48 million; p&gt; 0.05). After quality filtering, reads obtained from all samples were analyzed by rarefaction at equal depth. Alpha diversity measures were found to be significantly higher in the HEG group compared to the control group (Shannon, ACE, and Chao1 indices; p&lt; 0.05 for all). In the beta diversity analysis, it was observed that the gut microbiome compositions of the two groups were separated; In the Principal Coordinates Analysis plot, the groups were clearly clustered and the group difference was found to be statistically significant by PERMANOVA test (p&lt; 0.01). Significant differences were also found in the comparisons at the taxonomic level. At the class level, the relative abundance of Clostridia was significantly higher in the HEG group (p&lt; 0.05), while the class Bacilli was dominant in the control group (p&lt; 0.05). At the family level, the abundances of Lachnospiraceae and Prevotellaceae were found to be significantly higher in the HEG group than in the control group (p&lt; 0.05). In contrast, at the family level, the rates of Enterobacteriaceae and at the genus level, the rates of Escherichia-Shigella were found to be significantly higher in the control group (p&lt; 0.05). Some bacterial taxa detected only in the HEG group samples were also noteworthy: Collinsella, Blautia, and Dialister genera, which are only found in the intestines of patients with HEG, were not detected in the control group. In conclusion, these findings reveal that there are significant differences between the intestinal microbiome profiles of pregnant women with HEG and healthy pregnant women. The high microbial diversity observed in the HEG group and changes in certain bacterial groups suggest that processes related to lipid and carbohydrate metabolism may play a role in the pathogenesis of HEG. In the future, the clinical significance and possible therapeutic targets of these differences can be evaluated with more comprehensive studies aiming at clarifyin","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"60 1","pages":"53-71"},"PeriodicalIF":0.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Gastrointestinal PCR Panel Results and Evaluation of Inflammatory Biomarkers in Acute Gastroenteritis Cases].","authors":"Neslihan Çelik, Ömer Karaşahin, Rukiye Inan Sarikaya, Mehtap Hülya Aslan","doi":"10.5578/mb.202601112","DOIUrl":"10.5578/mb.202601112","url":null,"abstract":"<p><p>Acute gastroenteritis (AGE) is an important public health problem that is very common all over the world. Knowing the causative agents is important for understanding the clinical course of the disease, effective treatment and necessary precautions to be taken. In this study, we aimed to determine the pathogen distribution in AGE cases admitted to our center using multiplex real-time polymerase chain reaction (mRt-PCR) and to demonstrate the clinical and laboratory differences between luminal (type I) and mucosal/invasive (type II) infections. We also aimed to define biomarker thresholds to facilitate decision-making in situations where access to advanced diagnostic tests is limited. Eighty-five patients aged ≥ 18 years were retrospectively analyzed between June and September 2023. Clinical complaints, stool macroscopy and laboratory results recorded at admission including; leukocyte count, neutrophil/ lymphocyte ratio (NLR), hemoglobin, platelet count, C-reactive protein (CRP), renal and hepatic function tests and lactate dehydrogenase (LDH) values were recorded. Stool samples were evaluated by microscopic examination, culture/antigen tests and Bio-Speedy Gastroenteritis mRt-PCR (MX-24L). According to PCR results, the cases were categorized as a pathogen-negative group, a mucosal-type infection group and a luminal-type infection group. Appropriate statistical tests were used, ROC analysis was performed for CRP, NLR and platelet count to predict mucosal type. The median age was 54 years (interquartile range= 38-67) and 56.5% were male. At least one pathogen was detected by PCR in 76% (65/85) of the cases; the most common were Campylobacter spp. (17.3%), enteroinvasive Escherichia coli (12.9%) and norovirus (12.9%). 41.2% of the cases were mucosal type, 35.3% were luminal-type and 23.5% were in the group in which no causative agent was detected. Age was higher in the mucosal type group (p= 0.009). Red color/blood in stool and fever were significantly more common in mucosal type group (both p< 0.001); vomiting was more common in mucosal type group (p= 0.016). CRP and NLR values were significantly higher in mucosal type group (both p≤ 0.001), while platelet count was lower (p= 0.021). ROC analysis revealed CRP as the strongest predictor for mucosal type group. mRt-PCR enables high-rate detection of acute gastroenteritis pathogens, supporting accurate and early treatment and contributing to a reduction in unnecessary antibiotic use. By using this test, the most frequently identified acute gastroenteritis pathogens in our region were detected as Campylobacter, norovirus and EIEC. In settings where these tests are not available, CRP levels ≥ 42.5 mg/L and/or NLR ≥ 3 may serve as indicators in favor of mucosal-type pathogens and thrombocytopenia may further support this clinical profile. The use of simple and easily accessible biomarkers in combination with clinical findings may contribute to more effective field management of AGE cases.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"60 1","pages":"28-40"},"PeriodicalIF":0.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Cecal Tuberculosis Caused by Mycobacterium caprae Simulating Carcinoma: A Case Report and Review of the Literature].","authors":"Ali Mert, Abdurrahman Kaya","doi":"10.5578/mb.20260192","DOIUrl":"10.5578/mb.20260192","url":null,"abstract":"<p><p>Abdominal tuberculosis is a form of extrapulmonary tuberculosis that frequently involves the ileocecal region and often mimics conditions such as colorectal cancer or inflammatory bowel disease due to its nonspecific symptoms. While Mycobacterium tuberculosis remains the most common causative agent, rare zoonotic species like Mycobacterium caprae can also lead to human disease. In this case report, a rare case of cecal tuberculosis caused by M.caprae in a 46-year-old woman with a healthy immune system was presented. The patient presented chronic abdominal pain, diarrhea and weight loss for two years. Radiological imaging revealed ileocecal thickening and colonoscopy showed an ulcerovegetative mass raising suspicion of malignancy. Histopathological examination showed chronic granulomatous inflammation with acid-fast bacilli and molecular testing confirmed the presence of M.caprae. The patient was treated with standard anti-tuberculosis therapy including isoniazid, rifampicin, pyrazinamide and ethambutol. However, due to the development of post-treatment intestinal stenosis, surgical intervention was necessary. Human diseases with M.caprae are exceedingly rare and typically associated with occupational or environmental exposure to infected animals or unpasteurized dairy products. In this case, no such exposure was identified. The case highlights the diagnostic challenges posed by intestinal tuberculosis and underscores the importance of considering mycobacterial infections even in immunocompetent hosts. Early recognition and molecular identification are essential for accurate diagnosis and appropriate treatment. Clinicians should remain alert to potential complications such as fibrotic strictures that may require surgical management. This case represents one of the rare reports in the literature of cecal tuberculosis caused by M.caprae. To the best of our knowledge, reports describing similar cases are exceedingly rare.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"60 1","pages":"81-85"},"PeriodicalIF":0.8,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146086307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[In Vitro Assessment of the Antimicrobial and Antibiofilm Efficacy of Chlorhexidine, EDTA, and NaOCl in Combination with Nisin Against Enterococcus faecalis Isolates].","authors":"İrem Abay Celkan, Fatma Neslihan Özdemir, Nasrine Bekhedda, Fatemeh Mottaghizadeh, Nefise Akçelik","doi":"10.5578/mb.20250419","DOIUrl":"10.5578/mb.20250419","url":null,"abstract":"<p><p>Enterococcus faecalis is among the leading causes of hospital-acquired infections worldwide. This bacterium has both intrinsic and acquired resistance to various antibiotics. The continuous development\u0000of antibiotic resistance by pathogens poses a challenge that economically and technologically restricts\u0000the discovery of next-generation antibiotics. Therefore, many researchers aim to develop new strategies to combat this pathogen. The aim of this study was to evaluate the synergistic antimicrobial and\u0000antibiofilm effects of the combination of antimicrobial agents such as chlorhexidine (CHX), ethylenediaminetetraacetic acid (EDTA) and sodium hypochlorite (NaOCl) with nisin against clinical and food\u0000isolates of E.faecalis. Nisin produced by wild-type and recombinant Lactococcus lactis strains was purified\u0000and their activities were evaluated in comparison with commercial nisin. The minimum inhibitory concentration (MIC) and minimum biofilm eradication concentration (MBEC) of the agents were determined using microplate dilution methods. Antibiofilm activities were visualized using scanning electron\u0000microscopy (SEM). The MIC values for CHX, EDTA, and NaOCl were determined as 0.0002%, 5 mM, and\u00000.50%, respectively. The MIC values of nisin for all tested strains and isolates ranged from 12.5 to 100 IU/\u0000mL. Synergistic interactions of the antimicrobial agents were determined using the checkerboard method, revealing that the most effective combination was the combination of nisin and EDTA. Similarly, the\u0000most effective combination for eradicating 24-hour biofilms was nisin + EDTA (1000 IU/mL + 10 mM).\u0000The concentration of nisin in this combination was lower than its MBEC value (1500 IU/mL). While the\u0000combination of nisin with CHX and NaOCl achieved complete eradication of biofilms, the agents alone\u0000were ineffective in achieving such results. SEM images showed that although E.faecalis biofilms were not\u0000completely eradicated, they were significantly disrupted as a result of the combined application of nisin\u0000with antimicrobial agents. These in vitro findings indicate that the combination of nisin and EDTA may\u0000be effective against biofilm structures formed by clinical and food-derived E.faecalis isolates and demonstrate the potential of this combination for practical applications. Considering the different resistance\u0000profiles of clinical and food isolates, our study emphasizes the need to tailor treatment strategies according to the distinct characteristics of the bacterial strains involved. The results provide a scientific basis for\u0000future combination-based studies aimed at enhancing antibiofilm efficacy against E.faecalis.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"437-451"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Artesunate and Severe Malaria: The Importance of Proper Treatment Steps and Laboratory Monitoring].","authors":"Deniz Özer, İbrahim Çavuş, Ahmet Özbilgin","doi":"10.5578/mb.20250436","DOIUrl":"10.5578/mb.20250436","url":null,"abstract":"<p><p>Malaria is a severe infectious disease caused by Plasmodium parasites transmitted by infected female\u0000Anopheles mosquitoes. Plasmodium falciparum is the leading cause of malaria-related deaths worldwide\u0000and can lead to severe complications such as coma, metabolic acidosis and renal failure. In this report a\u0000case of high-parasitemia P.falciparum infection diagnosed and treated with intravenous (IV) artesunate\u0000was presented. The patient was a 52-year-old male with a travel history to Liberia who was referred\u0000to a university hospital due to thrombocytopenia and hematochezia. Subsequently, the patient developed severe malaria symptoms, including metabolic acidosis and acute renal failure. Laboratory tests\u0000revealed a leukocyte count of 9370/mm³, hemoglobin level of 18.3 g/dL, platelet count of 11000/μL,\u0000aspartate aminotransferase of 57 U/L, alanine aminotransferase of 28 U/L, gama-glutamyl transferase\u0000of 160 U/L, lactate dehydrogenase of 629 U/L, total bilirubin of 8 mg/dL, direct bilirubin of 4.64 mg/\u0000dL, creatinine of 1.69 mg/dL, prothrombin time of 14.9 seconds, international normalized ratio of 1.27,\u0000and C-reactive protein level of 247.7 mg/L. Giemsa-stained thin blood smear preparations, the Abbott\u0000Bioline™ Malaria Ag P.f/P.v rapid diagnostic test and real-time quantitative polymerase chain reaction\u0000confirmed P.falciparum infection. Intravenous artesunate and meropenem for secondary infections were\u0000administered, resulting in a rapid response to treatment. Parasite density was regularly monitored during\u0000treatment, and the patient was transitioned to oral therapy. According to the treatment algorithms of\u0000the World Health Organization and the Turkish Public Health Directorate, IV artesunate is the first-line\u0000treatment for severe malaria. In patients who have traveled to endemic regions and present with unexplained fever and flu-like symptoms, malaria should always be considered in the differential diagnosis and\u0000prompt diagnosis and treatment are crucial. Early diagnosis and the rapid action of artesunate significantly contribute to reducing morbidity and mortality rates. In this case, the rapid effect of artesunate\u0000and the significant decrease in parasitemia follow-up were clearly observed. In this case report, it was\u0000evaluated that parasitemia monitoring plays a critical role in directing patient treatment and is an important guide for clinicians and microbiologists.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"542-552"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401271","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[vanA/vanB Genes in Vancomycin-Susceptible Enterococcal Isolates: Molecular Identification and Evaluation of Diagnostic Methods].","authors":"Merve Gürler, Zeynep Ceren Karahan, Ebru Evren, Fazıl Alper Tekeli","doi":"10.5578/mb.20250473","DOIUrl":"10.5578/mb.20250473","url":null,"abstract":"<p><p>Vancomycin-variable enterococci (VVE) are Enterococcus strains that appear phenotypically susceptible\u0000to vancomycin but harbor glycopeptide resistance genes, representing a significant diagnostic and\u0000therapeutic challenge and posing a risk for therapeutic failure. These strains are often undetectable by\u0000conventional susceptibility methods but can be identified by molecular techniques. This study aimed to\u0000investigate the prevalence of vanA and vanB resistance genes in vancomycin- and teicoplanin-susceptible\u0000Enterococcus faecalis and Enterococcus faecium clinical isolates using multiplex and monoplex polymerase\u0000chain reaction (PCR) techniques. A total of 251 clinical Enterococcus isolates phenotypically confirmed\u0000as susceptible to vancomycin by disc diffusion test, broth microdilution and gradient diffusion test\u0000(Etest®), were retrospectively analyzed. Multiplex PCR targeting vanA and vanB genes was performed\u0000on all isolates, followed by monoplex PCR for the confirmation of positive results. Among the 251\u0000isolates screened, only one E.faecalis isolate (0.4%) from a urinary sample of a 91-year-old female patient\u0000detected as positive for the vanA gene in both multiplex and monoplex PCR. In contrast, vanB gene was\u0000detected as positive in 100 isolates (39.8%) by multiplex PCR, but none of these were confirmed by\u0000monoplex PCR. The detection of a vanA-positive E.faecalis isolate that was phenotypically susceptible to\u0000vancomycin highlights the risk of missing vancomycin-variable enterococci (VVE) when relying solely on\u0000conventional testing. The high rate of false-positive vanB results obtained by multiplex PCR demonstrates\u0000the limitations of this method and underscores the necessity of confirmatory testing within molecular\u0000workflows. Integrating gene-specific PCR assays into standard diagnostic protocols may facilitate more\u0000effective detection of VVE strains and the development of appropriate antimicrobial management\u0000strategies, particularly in settings with high glycopeptide use or unexplained treatment failures.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"425-436"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145400942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Determination of Class-1 and Class-2 Integron Gen Casettes in Klebsiella pneumoniae Causing Ventilator-associated Pneumonia].","authors":"Besim Çam, Elif Sevim, Lokman Hizmali","doi":"10.5578/mb.20250438","DOIUrl":"10.5578/mb.20250438","url":null,"abstract":"<p><p>Klebsiella pneumoniae is a major pathogen in ventilator-associated pneumonia (VAP) and poses a\u0000significant challenge in healthcare-associated infections due to its multidrug resistance (MDR). Mobile\u0000genetic elements such as integrons are key factors in the spread of resistance genes in pathogens like\u0000K.pneumoniae. This study aimed to detect class 1 and class 2 integron gene cassettes in K.pneumoniae\u0000isolates from VAP patients. A total of 22 isolates were collected between 2022 and 2023 from the intensive care unit of Kırşehir Training and Research Hospital. DNA was extracted using the boiling method\u0000and integrons were amplified via polymerase chain reaction with specific primers. Amplified fragments\u0000were cloned into the pJET1.2/blunt vector and sequenced. Sequences were processed using BioEdit\u0000and compared with the NCBI BLAST GenBank database to identify gene cassette contents. Identified\u0000sequences were submitted to GenBank and assigned accession numbers. Class 1 integrons were found\u0000in five isolates (22.7%) and class 2 integrons in two isolates (9.1%). Class 1 integrons harboured dfrA12\u0000responsible for trimethoprim resistance and aadA which provides resistance to aminoglycoside group antibiotics. In class 2 integrons, dfrA1, sat2 and aadA1 were identified which confer resistance to trimethoprim, streptothricin and streptomycin/spectinomycin, respectively. The results of this study demonstrate\u0000that integrons play a significant role in the spread of hospital-acquired MDR K.pneumoniae isolates.\u0000Strengthening infection control measures and monitoring antibiotic resistance mechanisms are crucial\u0000for controlling antibiotic-resistant isolates seen in hospitals. This study will contribute to understanding\u0000the genetic makeup of VAP-related K.pneumoniae isolates, determining appropriate treatment approaches and controlling hospital-acquired infections.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"508-516"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A Case of Fungal Sinusitis and Preseptal Cellulitis Caused by Lichtheimia corymbifera in a Patient with Acute Myeloid Leukemia].","authors":"Esra Tavukcu, Uğur Hatipoğlu, İpek Mumcuoğlu, Tuba Dal","doi":"10.5578/mb.20250422","DOIUrl":"10.5578/mb.20250422","url":null,"abstract":"<p><p>Lichtheimia corymbifera (formerly known as Absidia corymbifera) is a mold fungus belonging to the\u0000family Mucorales. Mucormycosis caused by L.corymbifera is a newly recognized and rare opportunistic\u0000infection in immunocompromised patients. Rapid diagnosis and treatment are crucial due to the high\u0000mortality rates, especially in patients with hematologic malignancies. Early clinical suspicion, surgical\u0000removal of necrotic tissue and appropriate antifungal chemotherapy constitute the main steps of treatment. Lipid formulations of Amphotericin B are the first-line treatment for mucormycosis. In this report\u0000a case of fungal sinusitis and preseptal cellulitis caused by L.corymbifera in a 23-year-old male patient\u0000who had been followed for eight years with a diagnosis of acute myeloid leukemia (AML) was presented.\u0000The patient, hospitalized due to relapsed AML, development of edema and discharge in the left eye and\u0000ophthalmological evaluation revealed preseptal cellulitis. Despite treatment, no clinical improvement\u0000was observed and contrast-enhanced orbital and paranasal sinus computed tomography revealed sinusitis. Direct microscopic examination of crusted lesions from the left nasal cavity showed the presence\u0000of irregular aseptate hyphae. A panic notification was reported to the relevant clinic with a preliminary\u0000diagnosis of mucormycosis. After the clinic and the patient interview, liposomal Amphotericin B treatment was started since the patient did not accept surgical debridement. The material was cultured on\u0000two Sabouraud’s Dextrose agar; one was incubated at 22 °C (room temperature) and the other at 37 °C\u0000(incubator). On the second day of incubation at 37 °C, colony morphology was observed as light white\u0000woolly surfaces with a dark white, non-pigmented base. In the preparations prepared with lactophenol\u0000cotton blue; fixed stolons ending in a pyriform funnel-shaped apophysis supporting the sporangium\u0000were observed. The agent grown in culture was named to species level with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) (MALDI Biotyper® Sirius System,\u0000Bruker, Germany) and diagnosed as L.corymbifera based on characteristic macroscopic and microscopic\u0000features. Although the patient refused surgical debridement treatment, early presumptive diagnosis allowed for the administration of liposomal Amphotericin B, resulting in successful treatment In conclusion,\u0000rapid preliminary diagnosis is critical to prevent the aggressive clinical course of mucormycosis, improves\u0000prognosis and increases survival rate.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"533-541"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Spinal Epidural Abscess and Spondylodiscitis Following Crimean-Congo Hemorrhagic Fever: A Rare Post-Recovery Complication].","authors":"Ömer Karaşahin, Neslihan Çelik, Nuh Çağrı Karaavci","doi":"10.5578/mb.20250458","DOIUrl":"10.5578/mb.20250458","url":null,"abstract":"<p><p>Crimean-Congo hemorrhagic fever (CCHF) is a rare but severe viral hemorrhagic disease transmitted\u0000to humans through tick bites or contact with the blood, tissues or secretions of infected animals. CCHF\u0000can lead to widespread endothelial damage and immune dysfunction, resulting in severe hemorrhagic\u0000manifestations and various complications. Although the majority of patients recover without permanent\u0000sequelae, unexpected complications such as secondary bacterial infections may rarely occur during the\u0000acute phase or convalescence. In this report, the case of a 42-year-old female patient who developed\u0000spinal epidural abscess and lumbar spondylodiscitis following real-time polymerase chain reaction testing\u0000confirmed CCHF was presented. On the seventh day of illness, the patient admitted to our emergency\u0000department with persistent high fever, nausea, vomiting, anorexia, malaise, diffuse body pain, hematuria and vaginal bleeding. The patient’s past medical history was unremarkable except for a lumbar disc\u0000herniation surgery 10 years ago. Following recovery from the acute viral illness, the patient developed\u0000sudden and severe lower back pain, recurrent high-grade fever and progressive motor weakness in plantar flexion of the right ankle. On the 10th day of hospitalization, spinal magnetic resonance imaging\u0000revealed spondylodiscitis and a spinal epidural abscess at the L5-S1 level. The epidural abscess caused\u0000compression of the S1 nerve roots, correlating with her neurological symptoms. The patient underwent\u0000surgical intervention (abscess drainage and stabilization) by the department of neurosurgery. Growth of\u0000Staphylococcus aureus in the blood culture confirmed that the infection was of bacterial origin but since\u0000the operation was performed while under 10 days of antibiotic treatment, there was no bacterial growth\u0000in the abscess material taken during the surgery. The patient was treated with intravenous cefazolin at a\u0000dose of 1000 mg three times daily for six weeks. Clinical improvement was rapid and complete, with no\u0000residual neurological deficits. This rare complication is thought to be related to immunoparalysis following a cytokine storm induced by CCHF, predisposing the patient to opportunistic bacterial infections.\u0000While leukopenia was observed during the immunosuppressive phase, a subsequent rise in leukocyte\u0000counts during recovery unmasked the signs of infection. Additionally, the patient’s history of prior spinal\u0000surgery may have created a localized microvascular injury or hematoma, forming a favorable niche for\u0000bacterial proliferation. New symptoms in the convalescent phase of CCHF must be carefully evaluated.\u0000In patients presenting with spinal pain and progressive localized motor deficits, prompt imaging and advanced diagnostics are essential to ensure early recognition and treatment of rare but potentially serious\u0000complications.</p>","PeriodicalId":18509,"journal":{"name":"Mikrobiyoloji bulteni","volume":"59 4","pages":"525-532"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145401227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}