Medical Science Monitor Basic Research最新文献_第2页

Study of 60 Adult Patients to Compare Standard Postoperative Clinical Assessment with Train-of-Four Ratio ≥0.9 on Patient Outcomes Using Postoperative Spirometry and Neuromuscular Function Measurements Following Extubation. 60例成人患者拔管后肺量测定和神经肌肉功能测量比较标准术后临床评估与四训练比值≥0.9患者预后的研究。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2023-03-08 DOI: 10.12659/MSMBR.938849

Chunlong Chen, Qingzhen Liu, Hong Fan, Zhiyang Yu, Xueyan Leng, Lidong Zhang, Zhiqiang Zhou

{"title":"Study of 60 Adult Patients to Compare Standard Postoperative Clinical Assessment with Train-of-Four Ratio ≥0.9 on Patient Outcomes Using Postoperative Spirometry and Neuromuscular Function Measurements Following Extubation.","authors":"Chunlong Chen, Qingzhen Liu, Hong Fan, Zhiyang Yu, Xueyan Leng, Lidong Zhang, Zhiqiang Zhou","doi":"10.12659/MSMBR.938849","DOIUrl":"https://doi.org/10.12659/MSMBR.938849","url":null,"abstract":"BACKGROUND Postoperative tracheal extubation requires optimal timing to ensure patient safety and normal muscle function. The train-of-four ratio (TOFR) of the fourth muscle response compared with the first indicates a non-depolarizing neuromuscular block, and a ratio ≥0.9 can be used as an objective measurement of neuromuscular reversal. This study of 60 adult patients who underwent elective surgery with general anesthesia that included the neuromuscular blocking agent cisatracurium aimed to compare standard postoperative clinical assessment with the TOFR ≥0.9 on patient outcomes using postoperative neuromuscular function assessed by grip strength and ability to sit up unaided and spirometry measurements following extubation. MATERIAL AND METHODS The 30 patients extubated postoperatively in the TOF group were required to have a TOFR ≥0.9, while the 30 patients in the clinical assessment group were awake and following simple commands and had a 5-second head lift and spontaneous breathing with acceptable oxygenation. The main outcomes were the incentive spirometry and grip strength and ability to sit up unaided measured at 10, 30, 50 min and 24 h after extubation. RESULTS The groups had no difference in recovery path of incentive spirometry volume (P=0.072) and no difference in postoperative incentive spirometry decrease from baseline except at 10 min after extubation (P=0.005). There was no difference in handgrip strength and independent sitting between groups. CONCLUSIONS The findings showed that using the TOF ratio ≥0.9 before extubation did not improve early postoperative strength quantified by spirometry volume, handgrip strength, and proportion of unaided sitting.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/86/f7/medscimonitbasicres-29-e938849.PMC10010054.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9118093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Prospective Study of 73 Patients to Compare Forward Head Angle, Forward Shoulder Angle, Maximal Inspiratory Pressure, and Self-Reported Breathing-Related Symptoms Before and After Open-Heart Surgery. 一项73例患者的前瞻性研究，比较前头角、前肩角、最大吸气压力和心内直视手术前后自我报告的呼吸相关症状。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2023-02-07 DOI: 10.12659/MSMBR.938802

Bussakorn Thanasarn, Wittawat Pibul, Chitima Kulchanarat, Dusarkorn Piathip, Kornanong Yuenyongchaiwat

{"title":"A Prospective Study of 73 Patients to Compare Forward Head Angle, Forward Shoulder Angle, Maximal Inspiratory Pressure, and Self-Reported Breathing-Related Symptoms Before and After Open-Heart Surgery.","authors":"Bussakorn Thanasarn, Wittawat Pibul, Chitima Kulchanarat, Dusarkorn Piathip, Kornanong Yuenyongchaiwat","doi":"10.12659/MSMBR.938802","DOIUrl":"https://doi.org/10.12659/MSMBR.938802","url":null,"abstract":"BACKGROUND Patients who undergo open-heart surgery often experience widespread musculoskeletal and pulmonary complications. These can interfere with their functioning, resulting in soft tissue changes, worsening postural changes, and poor respiratory performance. Therefore, the prospective study aimed to compare forward head angle (FHA) and forward shoulder angle (FSA), maximal inspiratory pressure (MIP), and self-reported breathing dysfunction before and after open-heart surgery. MATERIAL AND METHODS In a prospective observational study of 106 patients, men and women scheduled for open-heart surgery were enrolled. Prior to surgery and before discharge from the hospital, all patients were required to assess FHA and FSA using 2-dimensional motion analysis software, MIP using a respiratory pressure meter, and breathing dysfunction using the 25-item Self Evaluation of Breathing Questionnaire (SEBQ). Paired t test was used to compare differences between before and after surgery. To evaluate associations, logistic regression analysis was performed. RESULTS Of the 106 patients recruited, 73 completed the study. FHA (-Δ6.55±4.77, P.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/69/53/medscimonitbasicres-29-e938802.PMC9921079.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10804588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence and Associated Factors of Depression Among Frontline Nurses in Wuhan 6 Months After the Outbreak of COVID-19: A Cross-Sectional Study. 新冠肺炎疫情爆发后6个月武汉市一线护士抑郁患病率及相关因素的横断面研究

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2023-01-10 DOI: 10.12659/MSMBR.938633

Huan Liu, Zhiqing Zhou, Yan Liu, Xiubin Tao, Yuxin Zhan, Ming Zhang

{"title":"Prevalence and Associated Factors of Depression Among Frontline Nurses in Wuhan 6 Months After the Outbreak of COVID-19: A Cross-Sectional Study.","authors":"Huan Liu, Zhiqing Zhou, Yan Liu, Xiubin Tao, Yuxin Zhan, Ming Zhang","doi":"10.12659/MSMBR.938633","DOIUrl":"https://doi.org/10.12659/MSMBR.938633","url":null,"abstract":"BACKGROUND Due to the COVID-19 pandemic, frontline nurses have experienced psychological problems. However, the depression of frontline nurses in Wuhan 6 months after the outbreak of COVID-19 has not been studied adequately. The purpose of this study was to investigate the depression of frontline nurses in Wuhan 6 months after the COVID-19 outbreak and to analyze possible risk and protective factors. MATERIAL AND METHODS Data were collected through Wenjuanxing from 612 frontline nurses in Wuhan national COVID-19-designated hospitals between July 27, 2020, and August 12, 2020. The levels of depression, family functioning, and psychological resilience were assessed among frontline nurses in Wuhan with a depression scale, family function scale, and 10-item psychological resilience scale, respectively. The factors associated with depressive symptoms were identified using the chi-square test and binary logistic regression analysis. RESULTS A total of 126 respondents were included in the study. The overall prevalence of depression was 25.2%. The need for mental health services was a potential risk factor for depressive symptoms, while family functioning and psychological resilience were potential protective factors. The COVID-19 pandemic poses a major challenge to the depressive symptoms of frontline nursing in Wuhan, highlighting the need for all frontline nurses in Wuhan to be screened for depression regularly for timely intervention. CONCLUSIONS To mitigate the impact of the pandemic on depression, psychological interventions for frontline nurses need to be implemented to preserve their mental health.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2023-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/61/a1/medscimonitbasicres-29-e938633.PMC9840364.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10861023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Estimated Glomerular Filtration Rate and Urine Albumin-to-Creatinine Ratio Based on KDIGO 2012 Guideline in a Thai Community Hospital: Prevalence of Chronic Kidney Disease and its Risk Factors. 基于KDIGO 2012指南的肾小球滤过率和尿白蛋白与肌酐比值在泰国社区医院的应用:慢性肾病的患病率及其危险因素

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-12-08 DOI: 10.12659/MSMBR.938176

Veeravan Lekskulchai

{"title":"Use of Estimated Glomerular Filtration Rate and Urine Albumin-to-Creatinine Ratio Based on KDIGO 2012 Guideline in a Thai Community Hospital: Prevalence of Chronic Kidney Disease and its Risk Factors.","authors":"Veeravan Lekskulchai","doi":"10.12659/MSMBR.938176","DOIUrl":"https://doi.org/10.12659/MSMBR.938176","url":null,"abstract":"BACKGROUND Kidney disease is hard to detect at its early stage; therefore, the Kidney Disease: Improving Global Outcomes (KDIGO) 2012 guideline was developed for improving care and outcomes of patients with kidney disease. This study aimed to determine clinical outcomes from applying this guideline in a community hospital service. MATERIAL AND METHODS The patients' data were extracted from their medical records and analyzed for outcomes of using the estimated glomerular filtration rate (eGFR) and urine albumin-creatinine ratio (UACR) for detecting kidney disease. RESULTS The eGFR was utilized in 36 172 patients aged ≥18 years, and 76.86% of them had normal kidney function. The prevalence of chronic kidney disease (CKD) was 8.20%; most patients (68%) with CKD were in stages 3a and 3b. The most common causes of CKD were diabetes and hypertension. The UACR was mainly used in patients with diabetes. The percentage of patients with UACR ≥3 mg/mmol creatinine alone was significantly higher than that of patients with eGFR.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-12-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f3/f0/medscimonitbasicres-28-e938176.PMC9745955.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10424014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FLT3 Gene Mutations in Acute Myeloid Leukemia Patients in Northeast Thailand. 泰国东北部急性髓性白血病患者FLT3基因突变

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-11-24 DOI: 10.12659/MSMBR.937446

Nang Ei Ei Aung, Supawadee Yamsri, Nattiya Teawtrakul, Piyawan Kamsaen, Supan Fucharoen

{"title":"FLT3 Gene Mutations in Acute Myeloid Leukemia Patients in Northeast Thailand.","authors":"Nang Ei Ei Aung, Supawadee Yamsri, Nattiya Teawtrakul, Piyawan Kamsaen, Supan Fucharoen","doi":"10.12659/MSMBR.937446","DOIUrl":"https://doi.org/10.12659/MSMBR.937446","url":null,"abstract":"BACKGROUND Mutations in the FLT3 gene are associated with acute myeloid leukemia (AML). FLT3 mutations have been identified in approximately 30% of de novo AML patients, particularly those with typical karyotype and inferior prognosis. Therefore, we examined the frequencies of an internal tandem duplication (ITD) and missense mutations of the FLT3 gene and their associated clinical characteristics in patients with AML in northeast Thailand. MATERIAL AND METHODS The leftover bone marrow and/or peripheral blood specimens of 65 newly diagnosed AML patients recruited from Srinagarind Hospital, Khon Kaen University, northeast Thailand, between January 2020 and May 2021 were included in this study. FLT3-ITD and FLT3- tyrosine kinase domain (TKD) were amplified using PCR-related techniques. RESULTS The frequencies of FLT3-ITD and TKD were found to be 16.9% (11/65) and 10.8% (7/65), respectively. One patient had ITD and TKD mutations. The white blood cell count and peripheral blast percentage of FLT3-ITD-positive patients were statistically significantly higher than those of the FLT3-wild type patients, while the peripheral blast percentage of FLT3-TKD-positive patients was significantly lower. No other clinical characteristics among FLT3-positive and FLT3-wild-type patients were observed. DNA sequencing identified 4 FLT3-TKD mutations. The c.2504A>T; Asp835Val and c.2503G>C; Asp835His mutations were predicted as pathogenic mutations while the 2 novel mutations, c.2508C>A; Ile836= and c.2508C>G; Ile836Met were predicted as neutral mutations. CONCLUSIONS This study showed for the first time that FLT3-TKD mutation is common among northeast Thai AML patients. The data should prove useful for selecting efficacious targeted treatment plans for the patients.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9e/5d/medscimonitbasicres-28-e937446.PMC9707044.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10424013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Evaluation of Antimicrobial Effectiveness of Dental Cement Materials on Growth of Different Bacterial Strains. 牙骨水泥材料对不同菌种生长的抗菌效果评价。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-11-03 DOI: 10.12659/MSMBR.937893

Zana D Lila-Krasniqi, Rrezarta Bajrami Halili, Valeza Hamza, Sokol Krasniqi

引用次数: 1

Cytotoxicity, Apoptosis, Migration Inhibition, and Autophagy-Induced by Crude Ricin from Ricinus communis Seeds in A549 Lung Cancer Cell Lines. 蓖麻籽粗蓖麻毒素在 A549 肺癌细胞株中诱导的细胞毒性、细胞凋亡、迁移抑制和自噬作用

IF 1.5

Medical Science Monitor Basic Research Pub Date : 2022-07-07 DOI: 10.12659/MSMBR.936683

Irma Erika Herawati, Ronny Lesmana, Jutti Levita, Anas Subarnas

{"title":"Cytotoxicity, Apoptosis, Migration Inhibition, and Autophagy-Induced by Crude Ricin from Ricinus communis Seeds in A549 Lung Cancer Cell Lines.","authors":"Irma Erika Herawati, Ronny Lesmana, Jutti Levita, Anas Subarnas","doi":"10.12659/MSMBR.936683","DOIUrl":"10.12659/MSMBR.936683","url":null,"abstract":"BACKGROUND Ricin protein derived from Ricinus communis seeds is known to have a high toxicity to humans and animals. Several studies revealed that ricin, belonging to ribosome inactivating protein-I, has cytotoxic properties against various types of cancer cell lines. MATERIAL AND METHODS Crude ricin (CR) from the seeds of R. communis was investigated for its cytotoxicity on the A549 cancer cell lines using the MTS assay, and the cell death mechanism was explored using flow cytometry and Western blot methods. The cell migration was measured using a scratch/wound-healing method and the autophagy activity was explored using Western blotting. RESULTS CR showed cytotoxicity against A549 cancer cell lines, with an IC₅₀ of 40.94 ppm. CR induced apoptosis and necrosis, but apoptosis occurred more frequently than necrosis. Apoptosis induced by CR was mediated by the activation of caspase-9 and caspase-3. CR inhibited cell migration in a concentration- and time-dependent manner, with the highest effect occurred at the concentration of 1.0 ppm. The autophagic experiment showed that CR inhibited autophagy in A549 lung cancer cells by decreasing Beclin-1 levels while increasing Atg5 levels in a concentration-dependent manner and CR decreased LC3-II level while increasing p62 level. Cisplatin treatment also inhibited autophagy as it exhibited the same effect on those autophagic proteins as CR. CONCLUSIONS Our findings suggest that CR might be a potential candidate for anticancer drugs, but further study is needed to verify its anticancer properties.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":1.5,"publicationDate":"2022-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/af/medscimonitbasicres-28-e936683.PMC9275072.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40614489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Apical Leakage After Root Canal Obturation with Glass Ionomer, Resin, and Zinc Oxide Eugenol Sealers Combined with Thermafil. 玻璃离子、树脂、氧化锌丁香酚密封剂联合Thermafil对根管封闭后根尖渗漏的评价。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-06-15 DOI: 10.12659/MSMBR.936675

Violeta Vula, Miranda Stavileci, Nexhmije Ajeti, Vegim Vula, Astrit Kuçi, Kastriot Meqa

{"title":"Evaluation of Apical Leakage After Root Canal Obturation with Glass Ionomer, Resin, and Zinc Oxide Eugenol Sealers Combined with Thermafil.","authors":"Violeta Vula, Miranda Stavileci, Nexhmije Ajeti, Vegim Vula, Astrit Kuçi, Kastriot Meqa","doi":"10.12659/MSMBR.936675","DOIUrl":"https://doi.org/10.12659/MSMBR.936675","url":null,"abstract":"BACKGROUND A hermetic seal at the apical terminus is required for healthy periradicular tissue. Root canal obturation sealers that are used in endodontics are based on zinc oxide eugenol, calcium hydroxide, resins, glass ionomers, silicone, or bioceramics, but no optimal sealer material has been identified to date. Therefore, the aim of this in vitro study was to evaluate apical leakage after crown-down preparation and root canal obturation with Endomethasone N, glass ionomer cement, and EndoRez sealers. MATERIAL AND METHODS For this in vitro study, we tested 92 extracted human teeth, which were divided into 3 groups after a preparation technique and obturation with Endomethasone N sealer, glass ionomer cement, and EndoRez sealer in combination with Thermafil obturator. Apical leakage was evaluated and compared among the tested groups using a dye leakage method through a stereomicroscope. The values were measured from the apex to the coronal extent of dye penetration. For statistical analysis, the t test was used for comparison of the arithmetic averages of tested groups. RESULTS After preparation with rotary files, tested groups obturated with Thermafil obturator in combination with Endomethasone sealer showed higher average dye penetration than tested groups obturated with EndoRez and glass ionomer sealer. CONCLUSIONS Although all experimental groups showed dye leakage, the glass ionomer sealer in combination with Thermafil showed the least leakage, compared with EndoRez and Endomethason N.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3c/14/medscimonitbasicres-28-e936675.PMC9208302.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40410937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of Sealing Abilities Among Zinc Oxide Eugenol Root-Canal Filling Cement, Antibacterial Bioceramic Paste, and Epoxy Resin, using Enterococcus faecalis as a Microbial Tracer. 用粪肠球菌作为微生物示踪剂对氧化锌丁香酚根管充填水泥、抗菌生物陶瓷膏和环氧树脂封堵性能的比较。

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-06-01 DOI: 10.12659/MSMBR.936319

Tringa Kelmendi, Ferit Koçani, Arsim Kurti, Blerim Kamberi, Anila Kamberi

{"title":"Comparison of Sealing Abilities Among Zinc Oxide Eugenol Root-Canal Filling Cement, Antibacterial Bioceramic Paste, and Epoxy Resin, using Enterococcus faecalis as a Microbial Tracer.","authors":"Tringa Kelmendi, Ferit Koçani, Arsim Kurti, Blerim Kamberi, Anila Kamberi","doi":"10.12659/MSMBR.936319","DOIUrl":"https://doi.org/10.12659/MSMBR.936319","url":null,"abstract":"BACKGROUND This study aimed to compare sealing abilities among 3 root canal sealers, using Enterococcus faecalis as a microbial tracer. MATERIAL AND METHODS Sixty-six straight single-rooted teeth were subjected to crown removal at the cementoenamel junction. Root canals were instrumented using the crown-down technique. Prepared teeth were randomly divided into 3 groups of 22 teeth each, according to the sealer used: Canason zinc oxide eugenol root-canal filling cement, Well-Root ST antibacterial bioceramic paste, and AH 26 epoxy resin. Root canals were obturated using the lateral condensation technique, then stored at 37°C and 100% humidity for 1 week to allow sealers to set completely. Subsequently, bacterial leakage was tested using a 2-chamber method. Twice weekly, fresh E. faecalis was placed into the coronal portion of the filled root canal. Specimens were monitored at 24-h intervals for 33 days. The number of leaking samples and mean interval until leakage were compared among groups. RESULTS The mean intervals until leakage were 5.8 days in the Canason group, 1.5 days in the Well-Root ST group, and 13.3 days in the AH 26 group. These mean intervals significantly differed between the Canason and Well-Root ST groups (P=0.035) and between the AH 26 and Well-Root ST groups (P=0.012). After 33 days, survival rates were 31.8% in the Canason group, 90.9% in the Well-Root ST group, and 68.2% in the AH 26 group (P.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f2/ee/medscimonitbasicres-28-e936319.PMC9169682.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40411284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16–F10 Cells 骨化三醇抑制B16-F10细胞增殖并可能诱导凋亡

IF 2.8

Medical Science Monitor Basic Research Pub Date : 2022-05-05 DOI: 10.12659/MSMBR.935139

Eva Krishna Sutedja, Daniar Amarassaphira, H. Goenawan, Y. Pratiwi, N. Sylviana, B. Setiabudiawan, O. Suwarsa, R. Judistiani, U. Supratman, R. Lesmana

{"title":"Calcitriol Inhibits Proliferation and Potentially Induces Apoptosis in B16–F10 Cells","authors":"Eva Krishna Sutedja, Daniar Amarassaphira, H. Goenawan, Y. Pratiwi, N. Sylviana, B. Setiabudiawan, O. Suwarsa, R. Judistiani, U. Supratman, R. Lesmana","doi":"10.12659/MSMBR.935139","DOIUrl":"https://doi.org/10.12659/MSMBR.935139","url":null,"abstract":"Background Melanoma is one of the most aggressive types of cancer and it has shown a remarkable surge in incidence during the last 50 years. Melanoma has been projected to be continuously rising in the future. Therapy for advanced-type melanoma is still a challenge due to the low response rate and poor 10-year survival. Interestingly, several epidemiological and preclinical studies had reported that vitamin D deficiency was associated with disease progression in several cancer types. In vivo and in vitro studies revealed anti-proliferative, anti-angiogenic, apoptosis, and differentiation induction effects of calcitriol in various cancers. However, information on the effects of calcitriol (1,25(OH)2D3) on melanoma is still limited, and its mechanism remains unclear. Material/Methods In the present study, by utilizing B16–F10 cells, which is a melanoma cell line, we explored the anti-proliferative effect of calcitriol using cell viability assay, near-infrared imaging, expression of apoptosis-related genes using real-time polymerase chain reactions (PCR), and the expression of apoptosis proteins levels using western blot. In addition, we also assessed calcitriol uptake by B16–F10 cells using high-performance liquid chromatography (HPLC). Results We found that calcitriol inhibits melanoma cell proliferation with an IC50 of 93.88 ppm (0.24 μM), as shown by cell viability assay. Additionally, we showed that B16–F10 cells are capable of calcitriol uptake, with a peak uptake time at 60 min after administration. Calcitriol was also able to induce apoptosis-related proteins such as caspase-3, caspase 8, and caspase-9. These effects of calcitriol reflect its potential utility as a potent adjuvant therapy for melanoma. Conclusions Calcitriol inhibits cell proliferation and induces apoptosis in B16–F10 cells.","PeriodicalId":18491,"journal":{"name":"Medical Science Monitor Basic Research","volume":null,"pages":null},"PeriodicalIF":2.8,"publicationDate":"2022-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83991964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5