Microcirculation最新文献

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Perivascular Adipose Tissue and Uterine Artery Adaptations to Pregnancy 血管周围脂肪组织和子宫动脉对妊娠的适应。
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-06-03 DOI: 10.1111/micc.12857
Oluwatobiloba Osikoya, Nataliia Hula, Renée de Nazaré Oliveira da Silva, Styliani Goulopoulou
{"title":"Perivascular Adipose Tissue and Uterine Artery Adaptations to Pregnancy","authors":"Oluwatobiloba Osikoya,&nbsp;Nataliia Hula,&nbsp;Renée de Nazaré Oliveira da Silva,&nbsp;Styliani Goulopoulou","doi":"10.1111/micc.12857","DOIUrl":"10.1111/micc.12857","url":null,"abstract":"<p>Pregnancy is characterized by longitudinal maternal, physiological adaptations to support the development of a fetus. One of the cardinal maternal adaptations during a healthy pregnancy is a progressive increase in uterine artery blood flow. This facilitates sufficient blood supply for the development of the placenta and the growing fetus. Regional hemodynamic changes in the uterine circulation, such as a vast reduction in uterine artery resistance, are mainly facilitated by changes in uterine artery reactivity and myogenic tone along with remodeling of the uterine arteries. These regional changes in vascular reactivity have been attributed to pregnancy-induced adaptations of cell-to-cell communication mechanisms, with an emphasis on the interaction between endothelial and vascular smooth muscle cells. Perivascular adipose tissue (PVAT) is considered the fourth layer of the vascular wall and contributes to the regulation of vascular reactivity in most vascular beds and most species. This review focuses on mechanisms of uterine artery reactivity and the role of PVAT in pregnancy-induced maternal vascular adaptations, with an emphasis on the uterine circulation.</p>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/micc.12857","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141200279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Impact of Matrix Gel Variations on Primary Culture of Microvascular Endothelial Cell Function 基质凝胶变化对微血管内皮细胞原代培养功能的影响
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-31 DOI: 10.1111/micc.12859
Pía C. Burboa, Juliana C. Corrêa-Velloso, Cecilia Arriagada, Andrew P. Thomas, Walter N. Durán, Mauricio A. Lillo
{"title":"Impact of Matrix Gel Variations on Primary Culture of Microvascular Endothelial Cell Function","authors":"Pía C. Burboa,&nbsp;Juliana C. Corrêa-Velloso,&nbsp;Cecilia Arriagada,&nbsp;Andrew P. Thomas,&nbsp;Walter N. Durán,&nbsp;Mauricio A. Lillo","doi":"10.1111/micc.12859","DOIUrl":"10.1111/micc.12859","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>The endothelium regulates crucial aspects of vascular function, including hemostasis, vasomotor tone, proliferation, immune cell adhesion, and microvascular permeability. Endothelial cells (ECs), especially in arterioles, are pivotal for flow distribution and peripheral resistance regulation. Investigating vascular endothelium physiology, particularly in microvascular ECs, demands precise isolation and culturing techniques.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Freshly isolated ECs are vital for examining protein expression, ion channel behavior, and calcium dynamics. Establishing primary endothelial cell cultures is crucial for unraveling vascular functions and understanding intact microvessel endothelium roles. Despite the significance, detailed protocols and comparisons with intact vessels are scarce in microvascular research. We developed a reproducible method to isolate microvascular ECs, assessing substrate influence by cultivating cells on fibronectin and gelatin matrix gels. This comparative approach enhances our understanding of microvascular endothelial cell biology.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Microvascular mesenteric ECs expressed key markers (VE-cadherin and eNOS) in both matrix gels, confirming cell culture purity. Under uncoated conditions, ECs were undetected, whereas proteins linked to smooth muscle cells and fibroblasts were evident.</p>\u0000 \u0000 <p>Examining endothelial cell (EC) physiological dynamics on distinct matrix substrates revealed comparable cell length, shape, and Ca<sup>2+</sup> elevations in both male and female ECs on gelatin and fibronectin matrix gels. Gelatin-cultured ECs exhibited analogous membrane potential responses to acetylcholine (ACh) or adenosine triphosphate (ATP), contrasting with their fibronectin-cultured counterparts. In the absence of stimulation, fibronectin-cultured ECs displayed a more depolarized resting membrane potential than gelatin-cultured ECs.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Gelatin-cultured ECs demonstrated electrical behaviors akin to intact endothelium from mouse mesenteric arteries, thus advancing our understanding of endothelial cell behavior within diverse microenvironments.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11227414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141179888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcapillary PO2 Gradients in Contracting Muscles of Type I Diabetic Rats I 型糖尿病大鼠收缩肌肉中的跨毛细血管 PO2 梯度。
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-28 DOI: 10.1111/micc.12870
Ren Takamizawa, Kazuki Hotta, Yutaka Fujii, Ryo Ikegami, Naoki Hitosugi, Tatsuro Inoue, Hajime Tamiya, Atsuhiro Tsubaki
{"title":"Transcapillary PO2 Gradients in Contracting Muscles of Type I Diabetic Rats","authors":"Ren Takamizawa,&nbsp;Kazuki Hotta,&nbsp;Yutaka Fujii,&nbsp;Ryo Ikegami,&nbsp;Naoki Hitosugi,&nbsp;Tatsuro Inoue,&nbsp;Hajime Tamiya,&nbsp;Atsuhiro Tsubaki","doi":"10.1111/micc.12870","DOIUrl":"10.1111/micc.12870","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to clarify the effect of Type I diabetes (DIA) on transcapillary <i>P</i>O<sub>2</sub> gradients, which are oxygen-driving factors between the blood and the interstitium, in the contracting muscle of rats.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>Wistar male rats were divided into the diabetic (streptozocin i.p.) and sham groups. Microvascular and interstitial <i>P</i>O<sub>2</sub> were measured in the extensor digitorum longus muscle during electrical stimulation-induced muscle contraction, using the phosphorescence quenching method. Transcapillary <i>P</i>O<sub>2</sub> gradient, Δ<i>P</i>O<sub>2</sub>, was calculated as microvascular minus interstitial <i>P</i>O<sub>2</sub>.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Resting microvascular <i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group (6.3 ± 1.7 vs. 4.7 ± 0.9 mmHg, <i>p</i> &lt; 0.05) and remained for 180 s. Interstitial <i>P</i>O<sub>2</sub> from rest to muscle contraction did not differ between the groups. The Δ<i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group at rest and during muscle contraction (4.03 ± 1.42 vs. 2.46 ± 0.90 mmHg at rest; 3.67 ± 1.51 vs. 2.22 ± 0.65 mmHg during muscle contraction, <i>p</i> &lt; 0.05). Marked muscle atrophy was observed in the diabetic group.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>DIA increased microvascular and transcapillary <i>P</i>O<sub>2</sub> gradients in the skeletal muscle. The enhanced <i>P</i>O<sub>2</sub> gradients were maintained from rest to muscle contraction in diabetic muscle.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 6","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141162284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nicotine Impairs Smooth Muscle cAMP Signaling and Vascular Reactivity 尼古丁会损害平滑肌 cAMP 信号和血管反应性
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-28 DOI: 10.1111/micc.12871
Navid Singhrao, Victor A. Flores-Tamez, Yumna A. Moustafa, Gopireddy R. Reddy, Abby E. Burns, Kent E. Pinkerton, Chao-Yin Chen, Manuel F. Navedo, Madeline Nieves-Cintrón
{"title":"Nicotine Impairs Smooth Muscle cAMP Signaling and Vascular Reactivity","authors":"Navid Singhrao,&nbsp;Victor A. Flores-Tamez,&nbsp;Yumna A. Moustafa,&nbsp;Gopireddy R. Reddy,&nbsp;Abby E. Burns,&nbsp;Kent E. Pinkerton,&nbsp;Chao-Yin Chen,&nbsp;Manuel F. Navedo,&nbsp;Madeline Nieves-Cintrón","doi":"10.1111/micc.12871","DOIUrl":"10.1111/micc.12871","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>This study aimed to determine nicotine's impact on receptor-mediated cyclic adenosine monophosphate (cAMP) synthesis in vascular smooth muscle (VSM). We hypothesize that nicotine impairs β adrenergic–mediated cAMP signaling in VSM, leading to altered vascular reactivity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>The effects of nicotine on cAMP signaling and vascular function were systematically tested in aortic VSM cells and acutely isolated aortas from mice expressing the cAMP sensor <sup>T</sup>Epac<sup>VV</sup> (Camper), specifically in VSM (e.g., Camper<sub>SM</sub>).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>Isoproterenol (ISO)-induced β-adrenergic production of cAMP in VSM was significantly reduced in cells from second-hand smoke (SHS)–exposed mice and cultured wild-type VSM treated with nicotine. The decrease in cAMP synthesis caused by nicotine was verified in freshly isolated arteries from a mouse that had cAMP sensor expression in VSM (e.g., Camper<sub>SM</sub> mouse). Functionally, the changes in cAMP signaling in response to nicotine hindered ISO-induced vasodilation, but this was reversed by immediate PDE3 inhibition.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These results imply that nicotine alters VSM β adrenergic–mediated cAMP signaling and vasodilation, which may contribute to the dysregulation of vascular reactivity and the development of vascular complications for nicotine-containing product users.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 6","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11303104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141162281","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Striatal Blood Flow Changes by Middle Cerebral Artery Occlusion and Its Effect on Neurological Deficits in Mice 大脑中动脉闭塞导致的纹状体血流变化及其对小鼠神经功能缺损的影响
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-19 DOI: 10.1111/micc.12861
Miyuki Unekawa, Naoki Tsukada, Tsubasa Takizawa, Yutaka Tomita, Jin Nakahara, Yoshikane Izawa
{"title":"Striatal Blood Flow Changes by Middle Cerebral Artery Occlusion and Its Effect on Neurological Deficits in Mice","authors":"Miyuki Unekawa,&nbsp;Naoki Tsukada,&nbsp;Tsubasa Takizawa,&nbsp;Yutaka Tomita,&nbsp;Jin Nakahara,&nbsp;Yoshikane Izawa","doi":"10.1111/micc.12861","DOIUrl":"10.1111/micc.12861","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>We attempted to record the regional cerebral blood flow (CBF) simultaneously at various regions of the cerebral cortex and the striatum during middle cerebral artery (MCA) occlusion and to evaluate neurological deficits and infarct formation.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In male C57BL/6J mice, CBF was recorded in three regions including the ipsilateral cerebral cortex and the striatum with laser Doppler flowmeters, and the origin of MCA was occluded with a monofilament suture for 15–90 min. After 48 h, neurological deficits were evaluated, and infarct was examined by triphenyltetrazolium chloride (TTC) staining.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>CBF decrease in the striatum was approximately two-thirds of the MCA-dominant region of the cortex during MCA occlusion. The characteristic CBF fluctuation because of spontaneously occurred spreading depolarization observed throughout the cortex was not found in the striatum. Ischemic foci with slight lower staining to TTC were found in the ipsilateral striatum in MCA-occluded mice for longer than 30 min (<i>n</i> = 54). Twenty-nine among 64 MCA-occluded mice exhibited neurological deficits even in the absence of apparent infarct with minimum staining to TTC in the cortex, and the severity of neurological deficits was not correlated with the size of the cortical infarct.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Neurological deficits might be associated with the ischemic striatum rather than with cortical infarction.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 6","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/micc.12861","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141065951","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
In Silico Design of Heterogeneous Microvascular Trees Using Generative Adversarial Networks and Constrained Constructive Optimization 利用生成式对抗网络和受限构造优化技术设计异构微血管树的硅设计
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-01 DOI: 10.1111/micc.12854
Qing Pan, Huanghui Shen, Peilun Li, Biyun Lai, Akang Jiang, Wenjie Huang, Fei Lu, Hong Peng, Luping Fang, Wolfgang M. Kuebler, Axel R. Pries, Gangmin Ning
{"title":"In Silico Design of Heterogeneous Microvascular Trees Using Generative Adversarial Networks and Constrained Constructive Optimization","authors":"Qing Pan,&nbsp;Huanghui Shen,&nbsp;Peilun Li,&nbsp;Biyun Lai,&nbsp;Akang Jiang,&nbsp;Wenjie Huang,&nbsp;Fei Lu,&nbsp;Hong Peng,&nbsp;Luping Fang,&nbsp;Wolfgang M. Kuebler,&nbsp;Axel R. Pries,&nbsp;Gangmin Ning","doi":"10.1111/micc.12854","DOIUrl":"10.1111/micc.12854","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Designing physiologically adequate microvascular trees is of crucial relevance for bioengineering functional tissues and organs. Yet, currently available methods are poorly suited to replicate the morphological and topological heterogeneity of real microvascular trees because the parameters used to control tree generation are too simplistic to mimic results of the complex angiogenetic and structural adaptation processes in vivo.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We propose a method to overcome this limitation by integrating a conditional deep convolutional generative adversarial network (cDCGAN) with a local fractal dimension-oriented constrained constructive optimization (LFDO-CCO) strategy. The cDCGAN learns the patterns of real microvascular bifurcations allowing for their artificial replication. The LFDO-CCO strategy connects the generated bifurcations hierarchically to form microvascular trees with a vessel density corresponding to that observed in healthy tissues.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The generated artificial microvascular trees are consistent with real microvascular trees regarding characteristics such as fractal dimension, vascular density, and coefficient of variation of diameter, length, and tortuosity.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>These results support the adoption of the proposed strategy for the generation of artificial microvascular trees in tissue engineering as well as for computational modeling and simulations of microcirculatory physiology.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Significant Association of Serum Albumin With the Severity of Coronary Microvascular Dysfunction Using Dynamic CZT-SPECT 使用动态 CZT-SPECT 检测血清白蛋白与冠状动脉微血管功能障碍严重程度的关系
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-05-01 DOI: 10.1111/micc.12853
Shih-Chieh Chien, Shan-Ying Wang, Cheng-Ting Tsai, Yu-Chien Shiau, Yen-Wen Wu
{"title":"Significant Association of Serum Albumin With the Severity of Coronary Microvascular Dysfunction Using Dynamic CZT-SPECT","authors":"Shih-Chieh Chien,&nbsp;Shan-Ying Wang,&nbsp;Cheng-Ting Tsai,&nbsp;Yu-Chien Shiau,&nbsp;Yen-Wen Wu","doi":"10.1111/micc.12853","DOIUrl":"10.1111/micc.12853","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Objective</h3>\u0000 \u0000 <p>Both low serum albumin (SA) concentration and coronary microvascular dysfunction (CMD) are risk factors for the development of heart failure (HF). We hypothesized that SA concentration is associated with myocardial flow reserve (MFR) and implicated in pathophysiological mechanism of HF.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>We retrospectively studied 454 patients undergoing dynamic cardiac cadmium-zinc-telluride myocardial perfusion imaging from April 2018 to February 2020. The population was categorized into three groups according to SA level (g/dL): Group 1: &gt;4, Group 2: 3.5–4, and Group 3: &lt;3.5. Myocardial blood flow (MBF) and myocardial flow reserve (MFR, defined as stress/rest MBF ratio) were compared.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>The mean age of the whole cohort was 66.2 years, and 65.2% were men. As SA decreased, stress MBF (mL min<sup>−1</sup> g<sup>−1</sup>) and MFR decreased (MBF: 3.29 ± 1.03, MFR: 3.46 ± 1.33 in Group 1, MBF: 2.95 ± 1.13, MFR: 2.51 ± 0.93 in Group 2, and MBF: 2.64 ± 1.16, MFR: 1.90 ± 0.50 in Group 3), whereas rest MBF (mL min<sup>−1</sup> g<sup>−1</sup>) increased (MBF: 1.05 ± 0.42 in Group 1, 1.27 ± 0.56 in Group 2, and 1.41 ± 0.61 in Group 3). After adjusting for covariates, compared with Group 1, the odds ratios for impaired MFR (defined as MFR &lt; 2.5) were 3.57 (95% CI: 2.32–5.48) for Group 2 and 34.9 (95% CI: 13.23–92.14) for Group 3. The results would be similar if only regional MFR were assessed. The risk prediction for CMD using SA was acceptable, with an AUC of 0.76.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusion</h3>\u0000 \u0000 <p>Low SA concentration was associated with the severity of CMD in both global and regional MFR as well as MBF.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Vacuolar H+-ATPase in Diabetes, Hypertension, and Atherosclerosis 糖尿病、高血压和动脉粥样硬化中的空泡 H+-ATP 酶
IF 1.9 4区 医学
Microcirculation Pub Date : 2024-04-29 DOI: 10.1111/micc.12855
Na Wang, Liwei Ren, A. H. Jan Danser
{"title":"Vacuolar H+-ATPase in Diabetes, Hypertension, and Atherosclerosis","authors":"Na Wang,&nbsp;Liwei Ren,&nbsp;A. H. Jan Danser","doi":"10.1111/micc.12855","DOIUrl":"10.1111/micc.12855","url":null,"abstract":"<p>Vacuolar H<sup>+</sup>-ATPase (V-ATPase) is a multisubunit protein complex which, along with its accessory proteins, resides in almost every eukaryotic cell. It acts as a proton pump and as such is responsible for regulating pH in lysosomes, endosomes, and the extracellular space. Moreover, V-ATPase has been implicated in receptor-mediated signaling. Although numerous studies have explored the role of V-ATPase in cancer, osteoporosis, and neurodegenerative diseases, research on its involvement in vascular disease remains limited. Vascular diseases pose significant challenges to human health. This review aimed to shed light on the role of V-ATPase in hypertension and atherosclerosis. Furthermore, given that vascular complications are major complications of diabetes, this review also discusses the pathways through which V-ATPase may contribute to such complications. Beginning with an overview of the structure and function of V-ATPase in hypertension, atherosclerosis, and diabetes, this review ends by exploring the pharmacological potential of targeting V-ATPase.</p>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 5","pages":""},"PeriodicalIF":1.9,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/micc.12855","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140827965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolated Fragments of Intact Microvessels: Tissue Vascularization, Modeling, and Therapeutics 完整微血管的分离片段:组织血管化、建模和治疗
IF 2.4 4区 医学
Microcirculation Pub Date : 2024-04-15 DOI: 10.1111/micc.12852
Hannah A. Strobel, Sarah M. Moss, James B. Hoying
{"title":"Isolated Fragments of Intact Microvessels: Tissue Vascularization, Modeling, and Therapeutics","authors":"Hannah A. Strobel,&nbsp;Sarah M. Moss,&nbsp;James B. Hoying","doi":"10.1111/micc.12852","DOIUrl":"10.1111/micc.12852","url":null,"abstract":"<div>\u0000 \u0000 <p>The microvasculature is integral to nearly every tissue in the body, providing not only perfusion to and from the tissue, but also homing sites for immune cells, cellular niches for tissue dynamics, and cooperative interactions with other tissue elements. As a microtissue itself, the microvasculature is a composite of multiple cell types exquisitely organized into structures (individual vessel segments and extensive vessel networks) capable of considerable dynamics and plasticity. Consequently, it has been challenging to include a functional microvasculature in assembled or fabricated tissues. Isolated fragments of intact microvessels, which retain the cellular composition and structures of native microvessels, are proving effective in a variety of vascularization applications including tissue in vitro disease modeling, vascular biology, mechanistic discovery, and tissue prevascularization in regenerative therapeutics and grafting. In this review, we will discuss the importance of recapitulating native tissue biology and the successful vascularization applications of isolated microvessels.</p>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 4","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140595034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased Angio-Derived Index of Microcirculatory Resistance Within a Timeframe of 30–60 days After COVID-19 Infection 在 COVID-19 感染后 30-60 天内,血管衍生的微循环阻力指数增加。
IF 2.4 4区 医学
Microcirculation Pub Date : 2024-03-18 DOI: 10.1111/micc.12851
Lei Dong, Ritai Na, Lang Peng, Xinye Xu
{"title":"Increased Angio-Derived Index of Microcirculatory Resistance Within a Timeframe of 30–60 days After COVID-19 Infection","authors":"Lei Dong,&nbsp;Ritai Na,&nbsp;Lang Peng,&nbsp;Xinye Xu","doi":"10.1111/micc.12851","DOIUrl":"10.1111/micc.12851","url":null,"abstract":"<div>\u0000 \u0000 \u0000 <section>\u0000 \u0000 <h3> Background and Objectives</h3>\u0000 \u0000 <p>Chest pain is a relatively long-term symptom that commonly occurs in patients who have contracted COVID-19. The reasons for these symptoms remain unclear, with coronary microvascular dysfunction (CMD) emerging as a potential factor. This study aimed to assess the presence of CMD in these patients by measuring the angio-derived index of microcirculatory resistance (AMR).</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Methods</h3>\u0000 \u0000 <p>In this cross-sectional case–control study, patients who had chest pain and a history of COVID-19 infection within the preceding 30 to 60 days were included. The control subjects were patients without COVID-19. Demographic, clinical, and echocardiographic data were recorded. Angiographic images were collected for AMR analysis through an angioplus quantitative flow ratio measurement system. Propensity score matching (PSM) was performed to match the two groups. Multivariate logistic regression was used to examine the association between COVID-19 incidence and the increase in AMR (AMR &gt; 285 mmHg*s/m) after correction for other confounders.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Results</h3>\u0000 \u0000 <p>After PSM, there were 58 patients in each group (the mean age was 66.3 ± 9.04 years, and 55.2% were men). The average time between the onset of COVID-19 infection and patient presentation at the hospital for coronary angiography was 41 ± 9.5 days. Moreover, there was no significant difference in the quantitative flow ratio between the two groups. Patients with COVID-19 had a greater mean AMR (295 vs. 266, <i>p</i> = 0.002). Multivariate logistic regression analysis revealed that COVID-19 (OR = 3.32, 95% CI = 1.50–7.60, <i>p</i> = 0.004) was significantly associated with an increase in AMR.</p>\u0000 </section>\u0000 \u0000 <section>\u0000 \u0000 <h3> Conclusions</h3>\u0000 \u0000 <p>Long-term COVID-19 patients who experience chest pain without evidence of myocardial ischemia exhibit an increase in AMR, and CMD may be one of the reasons for this increase. COVID-19 is an independent risk factor for an increase in AMR.</p>\u0000 </section>\u0000 </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":"31 3","pages":""},"PeriodicalIF":2.4,"publicationDate":"2024-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140143763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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