{"title":"I 型糖尿病大鼠收缩肌肉中的跨毛细血管 PO2 梯度。","authors":"Ren Takamizawa, Kazuki Hotta, Yutaka Fujii, Ryo Ikegami, Naoki Hitosugi, Tatsuro Inoue, Hajime Tamiya, Atsuhiro Tsubaki","doi":"10.1111/micc.12870","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objective</h3>\n \n <p>This study aimed to clarify the effect of Type I diabetes (DIA) on transcapillary <i>P</i>O<sub>2</sub> gradients, which are oxygen-driving factors between the blood and the interstitium, in the contracting muscle of rats.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>Wistar male rats were divided into the diabetic (streptozocin i.p.) and sham groups. Microvascular and interstitial <i>P</i>O<sub>2</sub> were measured in the extensor digitorum longus muscle during electrical stimulation-induced muscle contraction, using the phosphorescence quenching method. Transcapillary <i>P</i>O<sub>2</sub> gradient, Δ<i>P</i>O<sub>2</sub>, was calculated as microvascular minus interstitial <i>P</i>O<sub>2</sub>.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Resting microvascular <i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group (6.3 ± 1.7 vs. 4.7 ± 0.9 mmHg, <i>p</i> < 0.05) and remained for 180 s. Interstitial <i>P</i>O<sub>2</sub> from rest to muscle contraction did not differ between the groups. The Δ<i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group at rest and during muscle contraction (4.03 ± 1.42 vs. 2.46 ± 0.90 mmHg at rest; 3.67 ± 1.51 vs. 2.22 ± 0.65 mmHg during muscle contraction, <i>p</i> < 0.05). Marked muscle atrophy was observed in the diabetic group.</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>DIA increased microvascular and transcapillary <i>P</i>O<sub>2</sub> gradients in the skeletal muscle. The enhanced <i>P</i>O<sub>2</sub> gradients were maintained from rest to muscle contraction in diabetic muscle.</p>\n </section>\n </div>","PeriodicalId":18459,"journal":{"name":"Microcirculation","volume":null,"pages":null},"PeriodicalIF":1.9000,"publicationDate":"2024-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Transcapillary PO2 Gradients in Contracting Muscles of Type I Diabetic Rats\",\"authors\":\"Ren Takamizawa, Kazuki Hotta, Yutaka Fujii, Ryo Ikegami, Naoki Hitosugi, Tatsuro Inoue, Hajime Tamiya, Atsuhiro Tsubaki\",\"doi\":\"10.1111/micc.12870\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div>\\n \\n \\n <section>\\n \\n <h3> Objective</h3>\\n \\n <p>This study aimed to clarify the effect of Type I diabetes (DIA) on transcapillary <i>P</i>O<sub>2</sub> gradients, which are oxygen-driving factors between the blood and the interstitium, in the contracting muscle of rats.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Methods</h3>\\n \\n <p>Wistar male rats were divided into the diabetic (streptozocin i.p.) and sham groups. Microvascular and interstitial <i>P</i>O<sub>2</sub> were measured in the extensor digitorum longus muscle during electrical stimulation-induced muscle contraction, using the phosphorescence quenching method. Transcapillary <i>P</i>O<sub>2</sub> gradient, Δ<i>P</i>O<sub>2</sub>, was calculated as microvascular minus interstitial <i>P</i>O<sub>2</sub>.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Results</h3>\\n \\n <p>Resting microvascular <i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group (6.3 ± 1.7 vs. 4.7 ± 0.9 mmHg, <i>p</i> < 0.05) and remained for 180 s. Interstitial <i>P</i>O<sub>2</sub> from rest to muscle contraction did not differ between the groups. The Δ<i>P</i>O<sub>2</sub> was higher in the diabetic group than in the sham group at rest and during muscle contraction (4.03 ± 1.42 vs. 2.46 ± 0.90 mmHg at rest; 3.67 ± 1.51 vs. 2.22 ± 0.65 mmHg during muscle contraction, <i>p</i> < 0.05). Marked muscle atrophy was observed in the diabetic group.</p>\\n </section>\\n \\n <section>\\n \\n <h3> Conclusion</h3>\\n \\n <p>DIA increased microvascular and transcapillary <i>P</i>O<sub>2</sub> gradients in the skeletal muscle. The enhanced <i>P</i>O<sub>2</sub> gradients were maintained from rest to muscle contraction in diabetic muscle.</p>\\n </section>\\n </div>\",\"PeriodicalId\":18459,\"journal\":{\"name\":\"Microcirculation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.9000,\"publicationDate\":\"2024-05-28\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microcirculation\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1111/micc.12870\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"HEMATOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microcirculation","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/micc.12870","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HEMATOLOGY","Score":null,"Total":0}
Transcapillary PO2 Gradients in Contracting Muscles of Type I Diabetic Rats
Objective
This study aimed to clarify the effect of Type I diabetes (DIA) on transcapillary PO2 gradients, which are oxygen-driving factors between the blood and the interstitium, in the contracting muscle of rats.
Methods
Wistar male rats were divided into the diabetic (streptozocin i.p.) and sham groups. Microvascular and interstitial PO2 were measured in the extensor digitorum longus muscle during electrical stimulation-induced muscle contraction, using the phosphorescence quenching method. Transcapillary PO2 gradient, ΔPO2, was calculated as microvascular minus interstitial PO2.
Results
Resting microvascular PO2 was higher in the diabetic group than in the sham group (6.3 ± 1.7 vs. 4.7 ± 0.9 mmHg, p < 0.05) and remained for 180 s. Interstitial PO2 from rest to muscle contraction did not differ between the groups. The ΔPO2 was higher in the diabetic group than in the sham group at rest and during muscle contraction (4.03 ± 1.42 vs. 2.46 ± 0.90 mmHg at rest; 3.67 ± 1.51 vs. 2.22 ± 0.65 mmHg during muscle contraction, p < 0.05). Marked muscle atrophy was observed in the diabetic group.
Conclusion
DIA increased microvascular and transcapillary PO2 gradients in the skeletal muscle. The enhanced PO2 gradients were maintained from rest to muscle contraction in diabetic muscle.
期刊介绍：
The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation.
Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
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