Striatal Blood Flow Changes by Middle Cerebral Artery Occlusion and Its Effect on Neurological Deficits in Mice

IF 1.9 4区 医学 Q3 HEMATOLOGY
Miyuki Unekawa, Naoki Tsukada, Tsubasa Takizawa, Yutaka Tomita, Jin Nakahara, Yoshikane Izawa
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Abstract

Objective

We attempted to record the regional cerebral blood flow (CBF) simultaneously at various regions of the cerebral cortex and the striatum during middle cerebral artery (MCA) occlusion and to evaluate neurological deficits and infarct formation.

Methods

In male C57BL/6J mice, CBF was recorded in three regions including the ipsilateral cerebral cortex and the striatum with laser Doppler flowmeters, and the origin of MCA was occluded with a monofilament suture for 15–90 min. After 48 h, neurological deficits were evaluated, and infarct was examined by triphenyltetrazolium chloride (TTC) staining.

Results

CBF decrease in the striatum was approximately two-thirds of the MCA-dominant region of the cortex during MCA occlusion. The characteristic CBF fluctuation because of spontaneously occurred spreading depolarization observed throughout the cortex was not found in the striatum. Ischemic foci with slight lower staining to TTC were found in the ipsilateral striatum in MCA-occluded mice for longer than 30 min (n = 54). Twenty-nine among 64 MCA-occluded mice exhibited neurological deficits even in the absence of apparent infarct with minimum staining to TTC in the cortex, and the severity of neurological deficits was not correlated with the size of the cortical infarct.

Conclusion

Neurological deficits might be associated with the ischemic striatum rather than with cortical infarction.

Abstract Image

大脑中动脉闭塞导致的纹状体血流变化及其对小鼠神经功能缺损的影响
研究目的我们尝试在大脑中动脉(MCA)闭塞时同时记录大脑皮层和纹状体各区域的脑血流(CBF),并评估神经功能缺损和梗死的形成:雄性C57BL/6J小鼠,用激光多普勒血流计记录同侧大脑皮层和纹状体等三个区域的CBF,用单丝缝线闭塞MCA起源15-90分钟。48 h后评估神经功能缺损,并用三苯基氯化四氮唑(TTC)染色检查梗死情况:结果:MCA闭塞期间,纹状体的CBF下降幅度约为皮层MCA优势区的三分之二。在整个大脑皮层观察到的因自发发生的扩散性去极化而导致的特征性 CBF 波动在纹状体中没有发现。在MCA闭塞超过30分钟的小鼠同侧纹状体中发现了TTC染色略低的缺血灶(n = 54)。在64只MCA闭锁小鼠中,有29只小鼠即使没有明显的梗死,也表现出神经功能缺损,皮质中TTC染色最低,神经功能缺损的严重程度与皮质梗死的大小无关:结论:神经功能缺损可能与缺血性纹状体而非皮质梗死有关。
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来源期刊
Microcirculation
Microcirculation 医学-外周血管病
CiteScore
5.00
自引率
4.20%
发文量
43
审稿时长
6-12 weeks
期刊介绍: The journal features original contributions that are the result of investigations contributing significant new information relating to the vascular and lymphatic microcirculation addressed at the intact animal, organ, cellular, or molecular level. Papers describe applications of the methods of physiology, biophysics, bioengineering, genetics, cell biology, biochemistry, and molecular biology to problems in microcirculation. Microcirculation also publishes state-of-the-art reviews that address frontier areas or new advances in technology in the fields of microcirculatory disease and function. Specific areas of interest include: Angiogenesis, growth and remodeling; Transport and exchange of gasses and solutes; Rheology and biorheology; Endothelial cell biology and metabolism; Interactions between endothelium, smooth muscle, parenchymal cells, leukocytes and platelets; Regulation of vasomotor tone; and Microvascular structures, imaging and morphometry. Papers also describe innovations in experimental techniques and instrumentation for studying all aspects of microcirculatory structure and function.
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