Membranes最新文献_第6页

A Novel Method for Separating Full and Empty Adeno-Associated Viral Capsids Using Ultrafiltration. 利用超滤技术分离完整和空腺病毒外壳的新方法。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-09-12 DOI: 10.3390/membranes14090194

Deepraj Sarmah, Scott M Husson

{"title":"A Novel Method for Separating Full and Empty Adeno-Associated Viral Capsids Using Ultrafiltration.","authors":"Deepraj Sarmah, Scott M Husson","doi":"10.3390/membranes14090194","DOIUrl":"https://doi.org/10.3390/membranes14090194","url":null,"abstract":"Adeno-associated viral vectors (AAVs) are the predominant viral vectors used for gene therapy applications. A significant challenge in obtaining effective doses is removing non-therapeutic empty viral capsids lacking DNA cargo. Current methods for separating full (gene-containing) and empty capsids are challenging to scale, produce low product yields, are slow, and are difficult to operationalize for continuous biomanufacturing. This communication demonstrates the feasibility of separating full and empty capsids by ultrafiltration. Separation performance was quantified by measuring the sieving coefficients for full and empty capsids using ELISA, qPCR, and an infectivity assay based on the live cell imaging of green fluorescent protein expression. We demonstrated that polycarbonate track-etched membranes with a pore size of 30 nm selectively permeated empty capsids to full capsids, with a high recovery yield (89%) for full capsids. The average sieving coefficients of full and empty capsids obtained through ELISA/qPCR were calculated as 0.25 and 0.49, indicating that empty capsids were about twice as permeable as full capsids. Establishing ultrafiltration as a viable unit operation for separating full and empty AAV capsids has implications for developing the scale-free continuous purification of AAVs.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434191/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How Is Bone Regeneration Influenced by Polymer Membranes? Insight into the Histological and Radiological Point of View in the Literature. 聚合物膜如何影响骨再生？从组织学和放射学角度洞察文献。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-09-11 DOI: 10.3390/membranes14090193

Alexandra Papuc, Simion Bran, Marioara Moldovan, Ondine Lucaciu, Gabriel Armencea, Grigore Baciut, Cristian Dinu, Florin Onișor, Winfried Kretschmer, Mihaela Baciut

{"title":"How Is Bone Regeneration Influenced by Polymer Membranes? Insight into the Histological and Radiological Point of View in the Literature.","authors":"Alexandra Papuc, Simion Bran, Marioara Moldovan, Ondine Lucaciu, Gabriel Armencea, Grigore Baciut, Cristian Dinu, Florin Onișor, Winfried Kretschmer, Mihaela Baciut","doi":"10.3390/membranes14090193","DOIUrl":"https://doi.org/10.3390/membranes14090193","url":null,"abstract":"The aim of this study was to analyze published works that investigate the in vivo bone regeneration capacity of polymeric membranes loaded with active substances and growth factors. This scoping review's purpose was to highlight the histological and radiological interpretation of the locally produced effects of the polymer membranes studied so far. For the selection of the articles, a search was made in the PubMed and ScienceDirect databases, according to the PRISMA algorithm, for research/clinical trial type studies. The search strategy was represented by the formula \"((biodegradable scaffolds AND critical bone defect) OR (polymers AND mechanical properties) OR (3Dmaterials AND cytotoxicity) AND bone tissue regeneration)\" for the PubMed database and \"((biodegradable scaffolds AND polymers) OR (polymers AND critical bone defects) OR (biodegradable scaffolds AND mechanical properties) AND bone tissue regeneration)\" for the ScienceDirect database. Ethical approval was not required. Eligibility criteria included eight clinical studies published between 2018 and 2023. Our analysis showed that polymer membranes that met most histopathological criteria also produced the most remarkable results observed radiologically. The top effective scaffolds were those containing active macromolecules released conditionally and staged. The PLGA and polycaprolactone scaffolds were found in this category; they granted a marked increase in bone density and improvement of osteoinduction. But, regardless of the membrane composition, all membranes implanted in created bone defects induced an inflammatory response in the first phase.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142336465","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coupling Low-Frequency Ultrasound to a Crossflow Microfiltration Pilot: Effect of Ultrasonic Pulse Application on Sono-Microfiltration of Jackfruit Juice. 将低频超声波耦合到错流微过滤试验中：超声波脉冲应用对柚子汁超声微滤的影响。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-09-11 DOI: 10.3390/membranes14090192

Herenia Adilene Miramontes-Escobar, Nicolas Hengl, Manuel Dornier, Efigenia Montalvo-González, Martina Alejandra Chacón-López, Nawel Achir, Fabrice Vaillant, Rosa Isela Ortiz-Basurto

{"title":"Coupling Low-Frequency Ultrasound to a Crossflow Microfiltration Pilot: Effect of Ultrasonic Pulse Application on Sono-Microfiltration of Jackfruit Juice.","authors":"Herenia Adilene Miramontes-Escobar, Nicolas Hengl, Manuel Dornier, Efigenia Montalvo-González, Martina Alejandra Chacón-López, Nawel Achir, Fabrice Vaillant, Rosa Isela Ortiz-Basurto","doi":"10.3390/membranes14090192","DOIUrl":"https://doi.org/10.3390/membranes14090192","url":null,"abstract":"To reduce membrane fouling during the processing of highly pulpy fruit juices into clarified beverages, a crossflow Sono-Microfiltration (SMF) system was employed, strategically equipped with an ultrasonic probe for the direct application of low-frequency ultrasound (LFUS) to the juice just before the entrance to the ceramic membrane. Operating conditions were standardized, and the application of LFUS pulses in both corrective and preventive modes was investigated. The effect of SMF on the physicochemical properties and the total soluble phenol (TSP) content of the clarified juice was also evaluated. The distance of ultrasonic energy irradiation guided the selection of the LFUS probe. Amplitude conditions and ultrasonic pulses were more effective in the preventive mode and did not cause membrane damage, reducing the operation time of jackfruit juice by up to 50% and increasing permeability by up to 81%. The SMF did not alter the physicochemical parameters of the clarified juice, and the measured LFUS energy ranges did not affect the TSP concentration during the process. This study is the first to apply LFUS directly to the feed stream in a pilot-scale crossflow microfiltration system to reduce the fouling of ceramic membranes and maintain bioactive compounds in jackfruit juice.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433797/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Whey Processing: Ultrafiltration, Nanofiltration, and Water Reuse from Diafiltration. 整合乳清加工：超滤、纳滤和渗滤水回用。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-09-06 DOI: 10.3390/membranes14090191

Vandré Barbosa Brião, Juliane Mossmann, Bruna Seguenka, Samarah Graciola, Jeferson Steffanello Piccin

引用次数: 0

Research on Reverse Osmosis (RO)/Nanofiltration (NF) Membranes Based on Thin Film Composite (TFC) Structures: Mechanism, Recent Progress and Application. 基于薄膜复合（TFC）结构的反渗透（RO）/纳滤（NF）膜研究：机理、最新进展和应用。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-09-05 DOI: 10.3390/membranes14090190

Huibin Geng, Weihao Zhang, Xiaoxu Zhao, Wei Shao, Haitao Wang

{"title":"Research on Reverse Osmosis (RO)/Nanofiltration (NF) Membranes Based on Thin Film Composite (TFC) Structures: Mechanism, Recent Progress and Application.","authors":"Huibin Geng, Weihao Zhang, Xiaoxu Zhao, Wei Shao, Haitao Wang","doi":"10.3390/membranes14090190","DOIUrl":"https://doi.org/10.3390/membranes14090190","url":null,"abstract":"The global shortage of clean water is a major problem, even in water-rich regions. To solve this problem, low-cost and energy-efficient water treatment methods are needed. Membrane separation technology (MST), as a separation method with low energy consumption, low cost, and good separation effect, has been widely used to deal with seawater desalination, resource recovery, industrial wastewater treatment, and other fields. With the continuous progress of scientific and technological innovation and the increasing demand for use, NF/RO membranes based on the TFC structure are constantly being upgraded. This paper presents the recent research progress of NF and RO membranes based on TFC structures and their applications in different fields, especially the formation mechanism and regulation of selective layer structures and the modification methods of selective layers. Our summary provides fundamental insights into the understanding of NF and RO membrane processes and hopefully triggers further thinking on the development of membrane filtration process optimization.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434543/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Flow Dynamics through a High Swelling Nanofiber Membrane Processed at Different Relative Humidities: A Study on a FexOy/Polyvinyl Alcohol Composite. 在不同相对湿度下加工的高膨胀纳米纤维膜的流动动力学：对 FexOy/Polyvinyl Alcohol 复合材料的研究。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-08-30 DOI: 10.3390/membranes14090189

Ayelen C Santos, Alicia Vergara-Rubio, Angel J Mazocca, Silvia Goyanes

{"title":"Flow Dynamics through a High Swelling Nanofiber Membrane Processed at Different Relative Humidities: A Study on a FexOy/Polyvinyl Alcohol Composite.","authors":"Ayelen C Santos, Alicia Vergara-Rubio, Angel J Mazocca, Silvia Goyanes","doi":"10.3390/membranes14090189","DOIUrl":"https://doi.org/10.3390/membranes14090189","url":null,"abstract":"Addressing the global problem of polluted water requires sustainable, efficient, and scalable remediation solutions, such as electrospun polyvinyl alcohol (PVA) membranes incorporating specific nanoadsorbents. The retention of contaminants depends on membrane swelling, morphology, and the adsorbent within the nanofiber. This study investigated the effect of relative humidity (RH) within the electrospinning chamber on the morphology of the resulting mats and how this affected the flow dynamics depending on whether or not the permeating liquid induced swelling in the membranes. An insolubilized PVA membrane was used as a hydrophilic filter model and a PVA membrane filled with iron oxide nanoparticles (IONPs) as a composite model (PVA + IONPs). The presence of IONPs increases the nanofiber diameter, which decreases when prepared under intermediate RH (IRH). Consequently, the nanofiber configuration, which is critical for filtration tortuosity, is influenced by RH. The initial swelling results in over 60% greater water flux through PVA + IONPs compared to PVA at an equivalent RH. This characterization helps to optimize membrane applications, highlighting that PVA + IONPs exhibit lower permeability values at IRH, indicating improved contaminant retention capabilities.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433868/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Carrier Phase Ultrafiltration and Backflow Recovery Technique for Purification of Biological Macromolecules. 用于纯化生物大分子的载体相超滤和回流回收技术。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-08-30 DOI: 10.3390/membranes14090188

Raja Ghosh

{"title":"A Carrier Phase Ultrafiltration and Backflow Recovery Technique for Purification of Biological Macromolecules.","authors":"Raja Ghosh","doi":"10.3390/membranes14090188","DOIUrl":"https://doi.org/10.3390/membranes14090188","url":null,"abstract":"A simple carrier phase based ultrafiltration technique that is akin to liquid chromatography and is suitable for medium-to-large volume sample preparation in the laboratory is discussed in this paper. A membrane module was integrated with a liquid chromatography system in a \"plug and play\" mode for ease of sample handling, and recovery of species retained by the membrane. The sample injector and pump were used for feed injection and for driving ultrafiltration, while the sensors and detectors were used for real-time monitoring of the separation process. The concentration of retained species was enriched by utilizing controlled concentration polarization. The recovery of the retained and enriched species was enhanced by backflow of carrier phase through the membrane using appropriate combination of valves. The backflow of carrier phase also cleaned the membrane and limited the extent of membrane fouling. Proof-of-concept of the proposed technique was provided by conducting different types of protein ultrafiltration experiments. The technique was shown to be suitable for carrying out protein fractionation, desalting, buffer exchange and concentration enrichment. Adoption of this approach is likely to make ultrafiltration easier to use for non-specialized users in biological research laboratories. Other advantages include enhanced product recovery, significant reduction in the number of diavolumes of buffer needed for conducting desalting and buffer exchange, minimal membrane fouling and the potential for repeated use of the same module for multiple separation cycles.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433950/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Practical Osmotic Agent for High-Degree Pharmaceutical Pre-Concentration by Organic Solvent Forward Osmosis. 通过有机溶剂正向渗透进行高度药物预浓缩的实用渗透剂。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-08-29 DOI: 10.3390/membranes14090187

Ryoichi Takada, Ryosuke Takagi, Zhaohuan Mai, Atsushi Matsuoka, Hideto Matsuyama

{"title":"Practical Osmotic Agent for High-Degree Pharmaceutical Pre-Concentration by Organic Solvent Forward Osmosis.","authors":"Ryoichi Takada, Ryosuke Takagi, Zhaohuan Mai, Atsushi Matsuoka, Hideto Matsuyama","doi":"10.3390/membranes14090187","DOIUrl":"https://doi.org/10.3390/membranes14090187","url":null,"abstract":"Pre-concentration can reduce the total production costs in the pharmaceutical industry. Organic solvent forward osmosis (OSFO) is a suitable pre-concentration method because of its nonthermal nature, low capital cost, and potential for achieving high-degree concentrations. In a previous study, we first demonstrated a high-degree OSFO concentration. Sucrose octaacetate (SoA) in MeOH was concentrated to 52 wt% using polyethylene glycol (PEG)-400 as the osmotic agent, but the concentrated solution had a concentration of 17% PEG-400 because of the reverse solute flux. This result does not meet the typical purity standards required for pharmaceutical production, indicating the need to determine a suitable osmotic agent that can be used for practical purposes. This study proposes a practical osmotic agent for OSFO pre-concentration. First, osmotic agents were screened from a practical perspective. Polypropylene glycol (PPG)-400 was selected, owing to its low toxicity, good solubility, and low viscosity. Subsequently, the OSFO concentration was demonstrated using PPG-400 as the osmotic agent. SoA in MeOH was concentrated from 9.4 wt% to 48 wt%. The final feed solution contained only 0.04 wt% PPG-400. This result is the first demonstration of successful pharmaceutical pre-concentration using OSFO that satisfies the typical purity requirement in pharmaceutical production.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11433671/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tuning the Properties of Polyvinylidene Fluoride/Alkali Lignin Membranes to Develop a Biocatalytic Membrane Reactor for an Organophosphorus Pesticide Degradation. 调整聚偏氟乙烯/碱木素膜的特性，开发用于有机磷农药降解的生物催化膜反应器。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-08-28 DOI: 10.3390/membranes14090186

Serena Regina, Giuseppe Vitola, Rosalinda Mazzei, Lidietta Giorno

{"title":"Tuning the Properties of Polyvinylidene Fluoride/Alkali Lignin Membranes to Develop a Biocatalytic Membrane Reactor for an Organophosphorus Pesticide Degradation.","authors":"Serena Regina, Giuseppe Vitola, Rosalinda Mazzei, Lidietta Giorno","doi":"10.3390/membranes14090186","DOIUrl":"https://doi.org/10.3390/membranes14090186","url":null,"abstract":"It has been observed that the immobilization of a phosphotriesterase enzyme (PTE) onto polyvinylidene fluoride (PVDF) membranes significantly decreased the enzyme activity, and this negative effect was attributed to the hydrophobic character of the membrane. The indirect indication of this reason was that the same enzyme immobilized on other membrane materials bearing hydrophilic character showed better performance. In this work, we provide direct evidence of the mechanism by immobilizing a PTE on a PVDF membrane hydrophilized by blending it with alkali lignin (AL). The PTE was immobilized on PVDF membrane by a covalent bond with the same procedure used in earlier studies to attribute changes in enzyme activity solely to the wettability properties (and not to the material chemistry). The activity of the PTE immobilized on the PVDF membrane hydrophilized with AL was 50% higher than that of the enzyme immobilized on the PVDF hydrophobic membrane. Further improvements of the membrane structure tailored for the development of a biocatalytic membrane reactor (BMR) were also promoted. In particular, the performance of the BMR was studied as a function of the thickness of the membrane, which allowed us to modulate the residence time into the enzyme-loaded membrane pores while maintaining the flow rate through the pores at a constant.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"14 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11434455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142349686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of Online Flow Cytometry for Early Biofouling Detection in Reverse Osmosis Membrane Systems. 应用在线流式细胞仪对反渗透膜系统进行早期生物污损检测。

IF 3.3 4区工程技术

Membranes Pub Date : 2024-08-27 DOI: 10.3390/membranes14090185

Laura Pulido Beltran, Johannes S Vrouwenvelder, Nadia Farhat

引用次数: 0