Membranes最新文献

Microplasma-Mediated Enhancement of FD-150 Uptake in HL-60 Cells. 微血浆介导增强HL-60细胞对FD-150的摄取。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-18 DOI: 10.3390/membranes15050156

Mahedi Hasan, Jaroslav Kristof, Abubakar Hamza Sadiq, Md Jahangir Alam, Sadia Afrin Rimi, Farhana Begum, Kazuo Shimizu

{"title":"Microplasma-Mediated Enhancement of FD-150 Uptake in HL-60 Cells.","authors":"Mahedi Hasan, Jaroslav Kristof, Abubakar Hamza Sadiq, Md Jahangir Alam, Sadia Afrin Rimi, Farhana Begum, Kazuo Shimizu","doi":"10.3390/membranes15050156","DOIUrl":"10.3390/membranes15050156","url":null,"abstract":"Lipids are the primary components of cell membranes, and their properties can be temporarily modified by microplasma-generated species to enhance drug uptake. The ability of microplasmas to influence membrane dynamics has made them effective tools for facilitating drug uptake into cells. Despite this, the effect of microplasma irradiation on cell membranes is yet to be investigated. We investigated the effects of microplasma irradiation on fluorescein isothiocyanate-dextran 150 (FD-150) uptake in Human Promyelocytic Leukemia (HL-60) cells, with the focus on transmembrane potential and lipid order changes. Plasma was applied to HL-60 cells for five, seven, and ten minutes. Fluorescence intensity measurements showed that an uptake of FD-150 increased with treatment time, before declining at ten minutes of treatment. Following treatment, transmembrane potential analysis indicated transient hyperpolarization followed by gradual depolarization until 60 min, corresponding to increased FD-150 absorption. Analysis of the lipid order showed a more disordered membrane state, with the most pronounced changes observed at ten minutes. The increase in lipid disorder increases membrane permeability while excessive disruption of the lipid order impairs cell viability. These findings demonstrate the potential of plasma-generated reactive species in modulating membrane characteristics for intracellular drug delivery.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113648/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preparation of Tannic Acid-Pectin Coated PVDF Membrane for High-Efficiency Separation of Oil and Water Emulsions. 单宁酸-果胶包覆PVDF膜高效分离油水乳液的制备。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-16 DOI: 10.3390/membranes15050155

Liangku Zhai, Jiuyun Cui, Lei Lu, Hailong Wang, Can Wei, Jirong Luo, Atian Xie

引用次数: 0

Uncovering the Mechanisms of Intracellular Membrane Trafficking by Reconstituted Membrane Systems. 通过重组膜系统揭示细胞膜内运输的机制。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-16 DOI: 10.3390/membranes15050154

Shuhan Chen, Yinghui Liu, Haijia Yu

引用次数: 0

Membrane ATPases and Mitochondrial Proteins in Fetal Cerebellum After Exposure to L-Glutamate During Gestation. 妊娠期暴露于l -谷氨酸后胎儿小脑膜atp酶和线粒体蛋白的变化。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-16 DOI: 10.3390/membranes15050152

Adrián Tejero, David Agustín León-Navarro, Mairena Martín

{"title":"Membrane ATPases and Mitochondrial Proteins in Fetal Cerebellum After Exposure to L-Glutamate During Gestation.","authors":"Adrián Tejero, David Agustín León-Navarro, Mairena Martín","doi":"10.3390/membranes15050152","DOIUrl":"10.3390/membranes15050152","url":null,"abstract":"L-Glutamate (L-Glu) and its salt derivatives are widely used in the food industry as flavor enhancers. Although the consumption of these compounds is generally considered safe, some studies suggest that chronically consuming L-Glu may be associated with various disorders. In this study, Wistar pregnant rats were treated daily with 1 g/L of L-Glu in their drinking water throughout the gestational period. OPA-1, DRP-1, and mitofusin 2-key proteins involved in mitochondrial fusion and fission-were analyzed by Western blot. The results showed that L-Glu exposure significantly decreased DRP-1 levels, while OPA-1 and mitofusin 2 levels were unaffected. This was accompanied by a notable decrease in mitochondrial complexes III and V. The activities of Mg2+-ATPase and Na+/K+-ATPase were also analyzed in fetal cerebellar plasma membranes. Maternal L-Glu intake significantly increased Mg2+-ATPase activity. Regarding Na+/K+-ATPase, the data showed that L-Glu exposure did not modulate the protein level or its activity. However, a positive interaction with glutamate receptors was observed in both activities, although neither AMPA nor NMDA receptors appeared to be involved. These results suggest that chronic maternal L-Glu intake during gestation modulates Mg2+-ATPase activity and protein markers of mitochondrial dynamics in the fetal cerebellum, which could affect neonatal development.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113180/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151058","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fast-Rising Electric Pulses by Reducing Membrane Tension for Efficient Membrane Electroporation. 通过降低膜张力实现膜电穿孔的快速上升电脉冲。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-16 DOI: 10.3390/membranes15050151

Ping Ye, Lulu Huang, Kuiwen Zhao

引用次数: 0

Mesoscopic Structure of Lipid Nanoparticles Studied by Small-Angle X-Ray Scattering: A Spherical Core-Triple Shell Model Analysis. 用小角x射线散射研究脂质纳米颗粒的介观结构：球核-三壳模型分析。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-16 DOI: 10.3390/membranes15050153

Hao Li, Panqi Song, Yiwen Li, Shuyang Tu, Mehwish Mehmood, Liang Chen, Na Li, Qiang Tian

{"title":"Mesoscopic Structure of Lipid Nanoparticles Studied by Small-Angle X-Ray Scattering: A Spherical Core-Triple Shell Model Analysis.","authors":"Hao Li, Panqi Song, Yiwen Li, Shuyang Tu, Mehwish Mehmood, Liang Chen, Na Li, Qiang Tian","doi":"10.3390/membranes15050153","DOIUrl":"10.3390/membranes15050153","url":null,"abstract":"Lipid nanoparticles (LNPs) are widely recognized as effective drug delivery systems for RNA therapeutics because their efficacy is critically dependent on structural organization. The mesoscopic architecture of these multicomponent systems, which is governed by interactions among ionizable lipids, structural lipids, nucleic acids, and stabilizers, dictates encapsulation efficiency, biodistribution, and therapeutic performance. Although small-angle X-Ray scattering (SAXS) enables nanostructure characterization, the absence of suitable analytical models has hindered LNP development. Here, we present a core-triple shell SAXS model that resolves LNP hierarchical organization, including the inner lipid layer, intermediate hydrophilic region, and outer PEG corona. For LNPs encapsulating mRNA, a Gaussian distribution model was implemented to characterize the quasi-periodic structure originating from the self-assembly of mRNA-ionizable lipid complexes. Validation studies employing Comirnaty-based LNPs demonstrated that controlled variation of nitrogen-to-phosphorus (N/P) ratios produced distinguishable structural features that establish quantitative correlations between N/P ratios and LNP mesoscopic assembled structure. The modeling framework provides pharmaceutical researchers with robust analytical tools for systematic stability assessment and precision formulation for the optimization of LNPs. These structural insights are expected to advance the development of next-generation RNA therapeutics by potentially enhancing their delivery efficiency and pharmacokinetic properties.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fabrication of Composite Membrane by Constructing Helical Carbon Nanotubes in Ceramic Support Channels for Efficient Emulsion Separation. 在陶瓷支撑通道中构建螺旋碳纳米管制备高效乳液分离复合膜。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-15 DOI: 10.3390/membranes15050150

Kai Yuan, Rizhi Chen, Yiqing Zeng

{"title":"Fabrication of Composite Membrane by Constructing Helical Carbon Nanotubes in Ceramic Support Channels for Efficient Emulsion Separation.","authors":"Kai Yuan, Rizhi Chen, Yiqing Zeng","doi":"10.3390/membranes15050150","DOIUrl":"10.3390/membranes15050150","url":null,"abstract":"Membrane technology has emerged as an effective solution for the purification of oily wastewater, particularly in the separation of oil-in-water (O/W) emulsions. However, challenges, such as membrane fouling and the development of robust ceramic membranes with superior stability, continue to limit their widespread application. In this work, helical carbon nanotubes (HCNTs) with interlocking structures were grown in ceramic support channels through the airflow-induced chemical vapor deposition (CVD) method to fabricate membrane material with high hydrophilicity and underwater oleophobicity. The influence of CVD parameters on the growth of HCNTs and the membrane separation performance for O/W emulsions were studied systematically. The optimal HCNTs-SiC composite membrane was prepared at 600 °C, featuring a pore size of 0.95 μm and flux of 229.29 L·m-2·h-1. This membrane demonstrated exceptional purification efficiency (99.99%) for a 500 ppm O/W emulsion, along with a stable flux of 32.48 L·m-2·h-1 under a transmembrane pressure (TMP) of 1.5 bar. Furthermore, the unique membrane structure and surface heterogeneity contributed to its long service life and excellent recovery capability. This work provides a novel strategy for designing high-performance ceramic membranes for oil-water separation, offering potential solutions to current limitations in membrane technology.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113641/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151047","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Ammonia Adsorption and Desorption Behavior of Nafion. Nafion对氨的吸附和解吸行为。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-14 DOI: 10.3390/membranes15050149

Dominik Sachse, Andreas Glüsen, Klaus Wippermann, Martin Müller, Uwe Rau, Ralf Peters

{"title":"The Ammonia Adsorption and Desorption Behavior of Nafion.","authors":"Dominik Sachse, Andreas Glüsen, Klaus Wippermann, Martin Müller, Uwe Rau, Ralf Peters","doi":"10.3390/membranes15050149","DOIUrl":"10.3390/membranes15050149","url":null,"abstract":"The electrochemical nitrogen reduction reaction (eNRR) for electrochemical ammonia (NH3) synthesis is considered a promising alternative to the energy-intensive and highly CO2-emitting Haber-Bosch process. In numerous experiments, the Nafion membrane has been used as an electrolyte or separator. However, Nafion adsorbs and desorbs NH3, leading to erroneous measurements and making reproducibility extremely difficult. This study systematically investigates the interaction between NH3 and Nafion, underscoring the strength of the interaction between ammonium-ions (NH4+) and protons (H+). We found that minute quantities of synthesized NH3 are prone to persist within the membrane, albeit without affecting the ion conductivity and resistivity of Nafion. Consequently, the removal of NH3 from the membrane can occur under conditions where synthesis is not viable. The objective of this work is to heighten awareness regarding the interaction between NH3 and Nafion and contribute to the attainment of reliable and reproducible outcomes in eNRRs.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12112899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coenzyme Q10 Enhances Resilience of Mitochondrial-like Membranes Against Amyloidogenic Peptides. 辅酶Q10增强线粒体样膜抗淀粉样肽的弹性。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-13 DOI: 10.3390/membranes15050148

Raina Marie Seychell, Adam El Saghir, Gianluca Farrugia, Neville Vassallo

{"title":"Coenzyme Q10 Enhances Resilience of Mitochondrial-like Membranes Against Amyloidogenic Peptides.","authors":"Raina Marie Seychell, Adam El Saghir, Gianluca Farrugia, Neville Vassallo","doi":"10.3390/membranes15050148","DOIUrl":"10.3390/membranes15050148","url":null,"abstract":"Mitochondria possess a double-membrane envelope which is susceptible to insult by pathogenic intracellular aggregates of amyloid-forming peptides, such as the amyloid-beta (1-42) (Aβ42) peptide and the human islet amyloid polypeptide (hIAPP). The molecular composition of membranes plays a pivotal role in regulating peptide aggregation and cytotoxicity. Therefore, we hypothesized that modifying the physicochemical properties of mitochondrial model membranes with a small molecule might act as a countermeasure against the formation of, and damage by, membrane-active amyloid peptides. To investigate this, we inserted the natural ubiquinone Coenzyme Q10 (CoQ10) in model mito-mimetic lipid vesicles, and studied how they interacted with Aβ42 and hIAPP peptide monomers and oligomers. Our results demonstrate that the membrane incorporation of CoQ10 significantly attenuated fibrillization of the peptides, whilst also making the membranes more resilient against peptide-induced permeabilization. Furthermore, these protective effects were linked with the ability of CoQ10 to enhance membrane packing in the inner acyl chain region, which increased the mechanical stability of the vesicle membranes. Based on our collective observations, we propose that mitochondrial resilience against toxic biomolecules implicit in protein misfolding disorders such as Alzheimer's disease and type-2 diabetes, could potentially be enhanced by increasing CoQ10 levels within mitochondria.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113080/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-Situ Measurement of Gas Permeability for Membranes in Water Electrolysis. 电解水膜透气性的原位测量。

IF 3.3 4区工程技术

Membranes Pub Date : 2025-05-13 DOI: 10.3390/membranes15050147

Shuaimin Li, Chuan Song, Li Xu, Yuxin Wang, Wen Zhang

{"title":"In-Situ Measurement of Gas Permeability for Membranes in Water Electrolysis.","authors":"Shuaimin Li, Chuan Song, Li Xu, Yuxin Wang, Wen Zhang","doi":"10.3390/membranes15050147","DOIUrl":"10.3390/membranes15050147","url":null,"abstract":"Water electrolysis (WE) is a green technology for producing hydrogen gas without the emission of carbon dioxide. The ideal membrane materials in WE should be capable of transporting ions quickly and have gas barrier properties in harsh work environments. However, currently, no desirable measurement method has been developed for evaluating the gas barrier behavior of the membranes. Hence, an in-situ electrochemical method is developed to measure the gas permeability of membranes in the actual electrolysis environment, with the supersaturated state of H2 in the electrolyte and H2 bubbles during the electrolysis process. Four membranes, including Zirfon (a state-of-the-art alkaline WE membrane), polyphenylene sulfide fabric (PPS, a commercial alkaline WE membrane), FAA-3-PK-75 (a commercial anion-exchange membrane), and BILP-PE (a home-made composite membrane) were employed as the standard samples to perform the electrochemical measurement under different current densities, temperatures, and electrolyte concentrations. The results show that an increase in electrolytic current density or temperature or a decrease in KOH concentration can increase the H2 permeability of the membrane. The two porous membranes, Zirfon and PPS, are more affected by the current density and KOH concentration, while the dense FAA-3-PK-75 and BILP-PE membranes have a stronger ability to hinder H2 permeation. Under the conditions of 80 °C, 30 wt.% KOH, 101 kPa, and 400 mA·cm-2, the hydrogen permeability (×1010 L·cm·cm-2·s-1) of Zirfon, PPS, FAA, and BILP-PE are 263, 367, 28.3, and 5.32, respectively.","PeriodicalId":18410,"journal":{"name":"Membranes","volume":"15 5","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12113548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144151056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0