International Journal of Medical Laboratory最新文献_第8页

Biofilm and Extended Spectrum Beta Lactamase Production amongst Uropathogenic Escherichia Coli Isolates at the University of Ilorin Teaching Hospital, Nigeria 尼日利亚伊洛林大学教学医院尿路致病性大肠杆菌分离株的生物膜和广谱β -内酰胺酶生产

International Journal of Medical Laboratory Pub Date : 2019-11-10 DOI: 10.18502/ijml.v6i4.1999

D. Amadu, C. Nwabuisi, Y. Usman, J. Mustapha, I. Abdullahi, A. Popoola

{"title":"Biofilm and Extended Spectrum Beta Lactamase Production amongst Uropathogenic Escherichia Coli Isolates at the University of Ilorin Teaching Hospital, Nigeria","authors":"D. Amadu, C. Nwabuisi, Y. Usman, J. Mustapha, I. Abdullahi, A. Popoola","doi":"10.18502/ijml.v6i4.1999","DOIUrl":"https://doi.org/10.18502/ijml.v6i4.1999","url":null,"abstract":"Background and Aims: Uropathogenic Escherichia coli (UPEC) are considered major reservoir for genes encoding antimicrobial resistance. The mechanism of resistance and persistence of UPEC has been attributed to the production of biofilm and Extended Beta Lactamase (ESBL). This hospital-based prospective study determined how biofilm and ESBL production facilitate antibacterial resistance amongst UPEC isolated from catheter urine of patients attending the University of Ilorin Teaching Hospital, Nigeria. Materials and Methods: Urine samples from 113 catheterized inpatients and outpatients were analysed. Female subjects accounted for 47 (41.6%) of the study population. Standard microbiological methods and Analytical Profile Index (API) 20E were used for the isolation and identification of UPEC. Tissue culture plate technique was used to demonstrate biofilm production potentials and double-disc synergy test was used to determine ESBL production. Results: Catheter associated urinary tract infection in this study was 70.8% of samples analysed. Of this, Escherichia coli, 44 (55.0%) was the most predominant. UPEC, biofilm and ESBL production amounted to 38.9%, 81.8% and 27.2%, respectively. ESBL production was significantly associated with degree of biofilm formation (p<0.005). Both strong and moderate biofilm producers showed the same level of resistance to ceftazidime (31.6%). Moderate biofilm producers were 46.7% resistant to cefriaxone. Resistance to Amoxillin-clauvanate significantly occurred in all grades of biofilm producers (p>0.05). Imipenem, however, was the most sensitive with no resistance by the UPEC. Conclusions: ESBL and biofilm production were associated with antibacterial resistance. The incidence of ESBL production amongst biofilm forming UPEC is of great public health concern.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133306386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Probiotic Bifidobacterium Lactis Bacteria Inhibit the Invasion Phenotype of Shigella Dysenteriae Induced By Invasion Plasmid Antigen C 益生菌乳酸双歧杆菌抑制侵袭质粒抗原C诱导的痢疾志贺氏菌侵袭表型

International Journal of Medical Laboratory Pub Date : 2019-11-10 DOI: 10.18502/ijml.v6i4.2001

Maryamsadat Mirfarsi, S. Mehrabian, E. Torbati

{"title":"Probiotic Bifidobacterium Lactis Bacteria Inhibit the Invasion Phenotype of Shigella Dysenteriae Induced By Invasion Plasmid Antigen C","authors":"Maryamsadat Mirfarsi, S. Mehrabian, E. Torbati","doi":"10.18502/ijml.v6i4.2001","DOIUrl":"https://doi.org/10.18502/ijml.v6i4.2001","url":null,"abstract":"Background and Aims: Shigellosis is an acute gastroenteritis and Invasion plasmid antigen C (IpaC) is the first effector protein for Shigella invasion of intestinal cells. Among lactic acid bacteria, Bifidobacterium lactis (B. lactis) has received increasing attention for protection of a potential host against gastrointestinal infections. The aim of this study was to investigate the inhibitory activity of B. lactis against Shigella dysenteriae (S. dysenteriae) harboring IpaC gene strains from clinical specimens. Materials and Methods: Sixty stool samples of patients with bloody diarrhea were collected from three teaching hospitals in Tehran and subjected to further analysis for the identification of Shigella colonies by biochemical tests. DNA was assessed for IpaC gene by polymeraese chain reaction (PCR). Furthermore, IpaC gene expression analysis by real-time PCR was carried out to investigate whether probiotic B. lactis can inhibit the invasion phenotype of S. dysenteriae induced by IpaC. Results: Analysis of gene expression in S. dysenteriae harboring IpaC gene strains showed the expression of IpaC gene in treated S. dysentery with B. lactis being much lower than that of non-treated group (p<0.000). The results revealed that B. lactis at the concentration of 500 μg/ml bears strong inhibitory activity on the growth of S. dysenteriae by decreasing IpaC expression. Conclusions: Our results revealed the positive role of B. lactis in reducing the expression of the ipaC gene and inhibition of epithelial cell invasion by S. dysenteriae. Therefore, probiotics can be used as a complementary biotherapeutic agent in severe Shigella infection. ","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"2020 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121345174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Protective Effect of Silymarin on LipopolysaccharideInduced Liver Toxicity in Male Wistar Rat 水飞蓟素对脂多糖所致雄性Wistar大鼠肝毒性的保护作用

International Journal of Medical Laboratory Pub Date : 2019-11-10 DOI: 10.18502/ijml.v6i4.2010

F. Nourbakhsh, Samaneh Borooni, E. Tajbakhsh

{"title":"The Protective Effect of Silymarin on LipopolysaccharideInduced Liver Toxicity in Male Wistar Rat","authors":"F. Nourbakhsh, Samaneh Borooni, E. Tajbakhsh","doi":"10.18502/ijml.v6i4.2010","DOIUrl":"https://doi.org/10.18502/ijml.v6i4.2010","url":null,"abstract":"Background and Aims: Lipopolysaccharide (LPS) triggers production of reactive oxygen species and inflammatory cytokines. Nowadays Silybum marianum has been shown to treat liver and gall bladder disorders, especially to protect the liver against poisoning from various toxins. Therefore, we decided to evaluate the protective effect of silymarin on LPS-induced liver toxicity in male Wistar rat. Materials and Methods: Totally, 40 male Wistar rats were divided into 4 groups (n=10 in each): The animals were treated with silymarin for two weeks before the biochemical tests. Apoptosis was assessed by evaluating the amount of proteins in liver tissues by western blotting. Results: LPS induced hepatotoxicity as evidenced by histopathological damages and biochemical abnormalities. Data showed that malondialdehyde level significantly increases in the liver of LPS-treated rats. Destructive effects of LPS on histopathological and biochemical parameters were improved. LPS also increased expression of Bax/Bcl2 ratio and activation of caspase 3, caspase 8 and caspase 9. Western blot analysis showed silymarin treatment inhibiting apoptosis stimulated by LPS in the liver (p<0.001). Conclusions: The results of this research demonstrated that silymarin can exert protective effects against toxic effects of LPS in rat liver. Antiinflammatory drug can play a protective role in attenuating the liver inflammation induced by LPS injection.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128888037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Genotyping of Mycobacterium Tuberculosis Isolated from Suspected Patients in Tehran in 2015-2017 2015-2017年德黑兰地区疑似结核分枝杆菌基因分型分析

International Journal of Medical Laboratory Pub Date : 2019-09-02 DOI: 10.18502/ijml.v6i3.1399

S. Zaker, Hanie Bagherifard

{"title":"Genotyping of Mycobacterium Tuberculosis Isolated from Suspected Patients in Tehran in 2015-2017","authors":"S. Zaker, Hanie Bagherifard","doi":"10.18502/ijml.v6i3.1399","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1399","url":null,"abstract":"Background and Aims: Unlike many global efforts to eradicate tuberculosis caused by Mycobacterium, it remains as a life-threatening infection with a worldwide incidence of 1.5 million cases each year. However, due to the lack of information about Mycobacterium tuberculosis characterization, more studies are required to evaluate strain diversity and epidemiology of tuberculosis to improve the therapeutic approaches. This study aimed to genotype the Mycobacterium tuberculosis isolated from suspected patients in Tehran, Iran through 2015-2017.Materials and Methods: In the current study, 30 isolates (sputum, broncho-alveolar lavage and biopsy) were collected from different tuberculosis patients at Massoud Clinical Lab of Tehran from 2015 to 2017. To find the single nucleotide polymorphisms and mutated regions, polymerase chain reaction (PCR) was performed on all the isolates to amplify the katG and gyrA genes. Then, PCR products were sequenced and analyzed.Results: The majority of isolates were assigned to PGG2 (90%), followed by PGG3 (10%) but no isolate belonging to PGG1 was found.Conclusions: Our findings demonstrate a remarkable epidemiological pattern of tuberculosis in Tehran. In group 2, isolates showed a considerably higher frequency compared to isolates in group 3, which is consistent with other findings reported in Iran. However, in contrast to other Iranian studies, no isolated strains were categorized in principal PGG1.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116872373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Detection of Adenoviruses in the Sinus Tissues of Patient by Nested-PCR in Shiraz, Southwest Iran 巢式pcr检测伊朗西南部设拉子地区患者鼻窦组织腺病毒

International Journal of Medical Laboratory Pub Date : 2019-09-02 DOI: 10.18502/ijml.v6i3.1400

Shahab Mahmoudvand, M. Kord, N. Pirbonyeh, A. Moattari, Somayeh Shokri, K. Zomorodian

{"title":"Molecular Detection of Adenoviruses in the Sinus Tissues of Patient by Nested-PCR in Shiraz, Southwest Iran","authors":"Shahab Mahmoudvand, M. Kord, N. Pirbonyeh, A. Moattari, Somayeh Shokri, K. Zomorodian","doi":"10.18502/ijml.v6i3.1400","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1400","url":null,"abstract":"Background and Aims: Rhinosinusitis is an inflammation of the mucous membrane that may be caused by infectious agents such as bacteria, fungi and viruses. Few studies have been carried out on the role of viruses in Rhinosinusitis patients .The aim of this study was the molecular detection of Adenoviruses in sinus tissues by nested polymerase chain reaction (PCR) in Shiraz.Materials and Methods: In the present study, 103 paraffin-embedded biopsy specimens of sinus tissues were subjected to DNA extraction and tested for adenovirus DNA using Nested PCR. The amplification of a β-globin gene by PCR-based method was used to confirm the quality of extracted DNA.Results: A total of 103 samples of sinus tissues were examined. Of these patients, 50 (48.54%) were male and the rest were female (51.46%). The patients’ age ranged between 2 and 82 years and the mean age was 42.15±1.56 years. The adenovirus DNA was detected in 13 of 103 (12.6%) samples.Conclusions: The results of this study showed that Adenoviruses have high prevalence in rhinosinusitis patients. As a results, it is an important to investigate clinical significance of viral infections especially Adeno viruses in these patients.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"90 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114099182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effects of Aqueous and Ethanolic Extracts of Myrtus Communis Leaves on Trophozoites and Cysts of Acanthamoeba: An In Vitro Study 桃金娘叶水提液和醇提液对棘阿米巴滋养体和囊泡影响的体外研究

International Journal of Medical Laboratory Pub Date : 2019-09-02 DOI: 10.18502/ijml.v6i3.1404

Tooran Nayeri, Fatemeh Ghaffarifa, F. Khoshzaban, Abdolhosein D Dalimi Asl, H. Mirzaian, F. Jameie

{"title":"Effects of Aqueous and Ethanolic Extracts of Myrtus Communis Leaves on Trophozoites and Cysts of Acanthamoeba: An In Vitro Study","authors":"Tooran Nayeri, Fatemeh Ghaffarifa, F. Khoshzaban, Abdolhosein D Dalimi Asl, H. Mirzaian, F. Jameie","doi":"10.18502/ijml.v6i3.1404","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1404","url":null,"abstract":"Background and Aims: Acanthamoeba is a ubiquitous amphizoic organism which can cause lethal diseases such as granulomatous amoebic encephalitis and unfortunately, the infection has now increased in the world. The aim here was to evaluate in vitro anti-Acanthamoeba properties of crude aqueous and ethanolic extracts of Myrtus communis.Materials and Methods: In this experimental research, a clinical isolate of Acanthamoeba was cultured and genotyped. The aqueous and ethanolic extracts of Myrtus communis were prepared. Then, various concentrations of Myrtus communis extracts (1.25, 2.5, 5, and 10 mg/ml) were tested at three different times (24, 48 and 72 hr) on trophozoites and cysts of Acanthamoeba in vitro. The viability of trophozoites or cysts was tested by trypan blue method. Unstained (viable) and stained (nonviable) parasites were evaluated by counting with a neobar lam.Results: The percentage of viablity of trophozoites and cysts after adding ethanolic extract of Myrtus communis was 0% and 8.62%, respectively. Moreover, at 10 mg/ml concentration of aqueous extract of Myrtus communis, 0% trophozoites and 31.10% cysts lived after 72 h.Conclusions: This extract can be used as a safe anti-Acanthamoeba agent against trophozoites and cysts of Acanthamoeba and further investigations are recommended to show the effects of this plant as an antiparasitic drug in animal models and volunteer infected people.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127213140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Use of Mesenchymal Adult Stem Cell for Cartilage Regeneration by Hydrogel 利用间充质成体干细胞水凝胶再生软骨

International Journal of Medical Laboratory Pub Date : 2019-09-02 DOI: 10.18502/ijml.v6i3.1403

M. Ghiasi, M. Mehdizadeh, A. Sharifi, F. Tafvizi, Sedigheh Safari, Neda Tekiyeh Maroof

{"title":"Use of Mesenchymal Adult Stem Cell for Cartilage Regeneration by Hydrogel","authors":"M. Ghiasi, M. Mehdizadeh, A. Sharifi, F. Tafvizi, Sedigheh Safari, Neda Tekiyeh Maroof","doi":"10.18502/ijml.v6i3.1403","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1403","url":null,"abstract":"Background and Aims: Cartilage is a very specific tissue, which does not have the capacity to heal and renew itself. Although the invention of the method of surgery with autologous chondrocyte transplantation, developed tools to treat the cartilage lesions, it couldn’t gain a great success due to problems such as damage to the area of donation. Using the mesenchymal stem cells derived from adipose and culturing and differentiating them on scaffolds was considered appropriate as a successful research and clinical strategy.Materials and Methods: In the present study, the mesenchymal stem cells were separated from adipose tissue and cultured in two scaffolds of fibrin glue and alginate medium. After 1, 7 and 14 days of cell differentiation, the survival ability of the differentiated cells were analyzed by Chondrogenic MTT. Moreover, type I and II collagen, aggrecan and Sox9 expression were measured via real time-polymerase chain reaction. In addition, cartilage reconstruction on scaffolds was shown by a histological investigation.Results: Our results showed that the expression of CD90 and CD105 as mesenchymal markers is at a high level whereas the expression of CD34 and CD45 reaches a low level. The LSD test demonstrated that there was no remarkable difference among the chondrogenic MTT, scaffolds groups and control in 7 and 14 days after cell differentiation (p<0.05), although, fibrin glue had the highest expression in chondrogenic gens.Conclusions: Finding suggests that in order to utilize a new strategy for tissue regeneration utilization of inherent scaffolds such as fibrin glue can act as a protector for mesenchymal stem cells.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122157505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Anti-Trichomonas Vaginalis Activity of Ethanolic Extracts of Medicago Sativa and Satureja Hortensis, In Vitro Study 紫花苜蓿和荆芥乙醇提取物抗阴道毛滴虫活性的体外研究

International Journal of Medical Laboratory Pub Date : 2019-09-02 DOI: 10.18502/ijml.v6i3.1397

F. Mirzaei, V. Raissi, Aref Teimouri, Parisa Mousavi, M. Mohaghegh, Mahdi Dehghan Manshadi, F. Zare, B. Esboei

引用次数: 1

The Effect of Follicular Fluid on the Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells 卵泡液对人骨髓间充质干细胞增殖和成骨分化的影响

International Journal of Medical Laboratory Pub Date : 2019-08-10 DOI: 10.18502/ijml.v6i3.1398

Atefeh Soltani, S. Abroun, M. R. Valojerdi, B. Vahidianfar, Elaheh Sadat Hosseini

{"title":"The Effect of Follicular Fluid on the Proliferation and Osteoblastic Differentiation of Human Bone Marrow Mesenchymal Stem Cells","authors":"Atefeh Soltani, S. Abroun, M. R. Valojerdi, B. Vahidianfar, Elaheh Sadat Hosseini","doi":"10.18502/ijml.v6i3.1398","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1398","url":null,"abstract":"Background and Aims: Bone marrow-derived mesenchymal stem cells (BM-MSCs) are a well-known source of multipotent adult stem cells. Despite using different methodologies of MSCs preparing for clinical applications, the top safest procedure to manipulate these cells, has not yet been determined. Recently, ex-vivo expansion of MSCs for their subsequent implantation, using some biological product, is suggested instead of fetal bovine serum (FBS). Previous studies have shown the effect of follicular fluid (FF) (a dynamic fluid in ovarian follicle) as an additive component in cell culture. Hence, this study aimed to decipher its role on the human BM-MSC proliferation.Materials and Methods: In this study, BM-MSCs at 3rd passage were cultivated in the presence of 20% FF (group I), 10% FF+ FBS 10% (group II) and FBS 20% as control group. The capacity of proliferation as calculating population doubling times and gene expression levels of stem cell factor, stromal cell-derived factor 1, and transforming growth factor beta were analyzed in osteogeneic media to examine the impacts of FF on osteogenesis of MSCs.Results: Our results corroborated an up-regulatory effect of FF on the proliferation of BM-MSCs by shorter population doubling times in the group II of treated cells and an increase in gene expression level of osteocalcin and transforming growth factor beta in the presence of higher concentrations of FF in cell culture FF 20% and 10%, respectively.Conclusions: FF is a potent mitogen for cell proliferation. FF may be an efficient substitution of FBS in ex-vivo cell culture, eliminating zoonotic infections and immunological reactions.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"3 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124367460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DNA Methylation and Its Role in the Development of Leukemia DNA甲基化及其在白血病发生中的作用

International Journal of Medical Laboratory Pub Date : 2019-08-10 DOI: 10.18502/ijml.v6i3.1396

P. Bagheri, Shahin Aghamiri, A. Jafarpour, M. Esmaili, Mohammad Haddadi Aghdam, F. Zaker, Soodeh Namjoo

{"title":"DNA Methylation and Its Role in the Development of Leukemia","authors":"P. Bagheri, Shahin Aghamiri, A. Jafarpour, M. Esmaili, Mohammad Haddadi Aghdam, F. Zaker, Soodeh Namjoo","doi":"10.18502/ijml.v6i3.1396","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1396","url":null,"abstract":"Epigenetic changes play an essential role in cancer pathogenesis. It has been established by next-generation sequencing that more than 50% of the human cancers carry mutations in mechanisms involved in the organization of the chromatin and epigenetic regulations. DNA methylation is among the most common epigenetic changes in leukemia. In contrast to DNA mutations which are passively inherited from DNA replication, epimutations, for example, the hypermethylation and epigenetic silencing of tumor suppressor genes, must be actively maintained because of being reversible. Actually, the reversibility of epimutations by small-molecule inhibitors provides the basis for the use of such inhibitors in new cancer therapy strategies. However, DNA methylation mechanism and its role in leukemia are not fully understood; there are some serious concerns about the use of these drugs. In this study, we will review the mechanisms of DNA methylation and the genes that are methylated in leukemia. Moreover, new interesting findings of the epigenetic changes causeed by adult T-cell leukemia/lymphoma have been fully discussed.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125095172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0