International Journal of Medical Laboratory最新文献_第9页

The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process 制备过程中孵育时间对VIII因子活性和稳定性的影响

International Journal of Medical Laboratory Pub Date : 2019-08-10 DOI: 10.18502/ijml.v6i3.1401

K. Kabir, H. Hosseini, Mehdy Jahed Zargar, Zeynab Mandeh, Fatemeh Amrollahi, Navid Farahmandian, E. Bahreini

{"title":"The Effect of Incubation Time on the Activity and Stability of Factor VIII during the Preparation Process","authors":"K. Kabir, H. Hosseini, Mehdy Jahed Zargar, Zeynab Mandeh, Fatemeh Amrollahi, Navid Farahmandian, E. Bahreini","doi":"10.18502/ijml.v6i3.1401","DOIUrl":"https://doi.org/10.18502/ijml.v6i3.1401","url":null,"abstract":"Background and Aims: Hemophilia is a rare autoimmune disorder caused by autoantibodies directed in the majority of the cases against clotting factor VIII (FVIII). FVIII is extracted from human plasma or engineered from mammalian cell cultures using recombinant DNA technology. In Iran, most of the used FVIII is prepared from human plasma in Iranian Blood Transfusion Organization. It was seen important to estimate its stability and activity from bleeding time until product preparation.Material and Methods: In the analytical study, 60 healthy male donors (20-50 years old), 15 donors from each blood group, were selected after obtaining informed consent. Donors' blood was collected in QUADRI-PACKs and centrifuged after keeping at 24°C for 2 hours. The separated plasma was divided into three groups and incubated in the lab (22°C) for 0, 90, and 180 minutes, respectively. Then, samples were stored at -20°C for one month. Afterward, the plasma was thawed, and FVIII activity was assayed.Results: The activity of FVIII significantly (p<0.05) reduced by delay in freezing; after the time of 0 min: 134.84%±42, after 90 min: 126.88%±38, after 180 min: 120.22%±34. At all incubation times, the highest and the lowest FVIII activity were observed in A and O blood groups, respectively (p<0.05). FVIII activity was increased along with increasing age up to 35-40, but it decreased in subjects of 40-50 years old. These experiments confirmed that the longer the delay in freezing fresh frozen plasma, the greater the decrease in FVIII stability.Conclusions:. According to the results of this study, the best blood donors for FVIII product are those with blood group A in the age range of 40-35 years.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116476551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression Profiles of miR-93 and miR-330 in Iranian Patients with Chronic Lymphocytic Leukemia miR-93和miR-330在伊朗慢性淋巴细胞白血病患者中的表达谱

International Journal of Medical Laboratory Pub Date : 2019-06-01 DOI: 10.18502/IJML.V6I2.1027

Behnaz Nateghi, Elahe Shams, P. Behshood, S. Fathullahzadeh, Mansoor Salehi

{"title":"Expression Profiles of miR-93 and miR-330 in Iranian Patients with Chronic Lymphocytic Leukemia","authors":"Behnaz Nateghi, Elahe Shams, P. Behshood, S. Fathullahzadeh, Mansoor Salehi","doi":"10.18502/IJML.V6I2.1027","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1027","url":null,"abstract":"Background and Aims: Chronic lymphocytic leukemia (CLL) is the most common adult human leukemia. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression. Research has shown that in CLL, microRNAs can have function as oncogenes or tumor suppressors. Some studies demonstrated that the expression of microRNA-93 (miR-93) and microRNA-330 (miR-330) have been changed in several cancers, including lung, prostate, and colon cancer. We aimed to elucidate the changes in miR-93 and miR-330 expression in CLL patients in comparison with controls. \u0000Materials and Methods: In this case-control study, the expression levels of miR-93 and miR-330 was evaluated in 30 CLL patients who had referred to Omid Hospital, Isfahan, Iran, and 30 controls in peripheral blood mononuclear cells using reverse transcription quantitative PCR (RT-qPCR). \u0000Results: The expression of miR-93 and miR-330 were found to significantly increase in CLL patients compared with controls (p<0.0001). \u0000Conclusions: The ﬁndings indicated that miR-93 and miR-330 are probably the novel potential biomarker for early diagnosis of CLL, at least in Iranian patients. However, for a decisive result, further investigations are warranted","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"126 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127069943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Evaluation of Glutamate Dehydrogenase Activity and Insulin Secretion in Mice Exposed to Dexamethasone 地塞米松对小鼠谷氨酸脱氢酶活性和胰岛素分泌的影响

International Journal of Medical Laboratory Pub Date : 2019-06-01 DOI: 10.18502/IJML.V6I2.1026

H. Jamshidi, E. Ebrahimi

{"title":"Evaluation of Glutamate Dehydrogenase Activity and Insulin Secretion in Mice Exposed to Dexamethasone","authors":"H. Jamshidi, E. Ebrahimi","doi":"10.18502/IJML.V6I2.1026","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1026","url":null,"abstract":"Background and Aims: Diabetes is one of the most important endocrine disrupters and is associated with various hormones, including those that can lead to diabetes. Glucocorticoid use may lead to insulin resistance. Dexamethasone is one of these glucocorticoid compounds. Glutamate dehydrogenase plays a key role in the production of glutamate in the secretion of insulin. Based on these hormonal interactions, the aim of this study was to determine the activity of glutamate dehydrogenase and insulin secretion in dexamethasone-exposed mice. \u0000Materials and Methods: Twenty eight mice were divided into 4 experimental groups. Group 1 received standard normal saline as a control. Group 2 received standard food and received 1 mg / kg dexamethasone per day. Group 3 received standard diet and dexamethasone 3 mg / kg / day and group 4 with standard diet 5 mg / kg dexamethasone per day. After 21 days, the animals were killed, the pancreas and glutamate dehydrogenase, insulin, and serum glucose levels were determined. \u0000Results: Dexamethasone increased serum glucose levels significantly (P <0.05). Dexamethasone increased the glutamate dehydrogenase activity and insulin levels in dexamethasone treated mice (p<0.05) \u0000Conclusions: These results suggestd that dexamethasone increases glucose which leads to elevating glutamate dehydrogenase activity, and then increasing insulin. However, insulin was not enough to normalize glucose levels and led to hyperglycemia. Therefore, it is suggested to reduce dexamethasone administration.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121057938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antileishmanial Activity of Carum Copticum Essential Oil Against Leishmania Major [MRHO/IR/75/ER]: An In Vitro Study 黄连精油抗利什曼原虫活性的体外研究[MRHO/IR/75/ER

International Journal of Medical Laboratory Pub Date : 2019-06-01 DOI: 10.18502/IJML.V6I2.1031

Ali - Fattahi Bafghi, Moneyreh Modarres Mosadegh, M. Ghaemi, S. Hejazian

{"title":"Antileishmanial Activity of Carum Copticum Essential Oil Against Leishmania Major [MRHO/IR/75/ER]: An In Vitro Study","authors":"Ali - Fattahi Bafghi, Moneyreh Modarres Mosadegh, M. Ghaemi, S. Hejazian","doi":"10.18502/IJML.V6I2.1031","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1031","url":null,"abstract":"Background and Aims: Because of the toxicity and side-effects of synthetic drugs, there is a growing interest in biomedical plants. The aim of this study was to evaluate in vitro antileishmanial activity of Carum copticum essential oil against Leishmania (L) major. \u0000Materials and Methods: Nineteen experimental groups were designed to determine the effect of Carum copticum essential oil against L. major and compare it with Meglumine antimonite. Group 1 was the control group and included 200 µl of RPMI 1640 plus 2×105 cells/ml promastigotes. Groups 2-10 included the aforementioned substances plus 10 µl of 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2 and 3 µg/ml of Carum copticum essential oil respectively. Groups 11-19 were similar to groups 2-10 but Meglumine antimonite (0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1, 2 and 3 µg/ml) was used instead of Carum copticum essential oil. All the experiments were repeated five times. After 8 hours, the antileishmanial activities of studied substances were determined. \u0000Results: Up to concentration of 0.5 µg/ml, no effect was observed with both substances. In comparison to control group, at 1 and 2 µg/ml, Meglumine antimonite had no effect on Leishmaniasis (p>0.05) while Carum copticum essential oil significantly decreased Leishmaniasis viability (p<0.05). Moreover, at 3 µg/ml, both compounds significantly decreased Leishmaniasis viability (p<0.05). However, Carum copticum essential oil had substantially better Antileishmanial activity than the other. \u0000Conclusions: These results suggest that comparable concentrations, in vitro antileishmanial activity of Carum copticum essential oil is better than Meglumine antimonite.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123537425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Effect of Biochanin A as PPAR γ agonist on LDL Particles Diameter and Type 2 Diabetic Dyslipidemia 生物茶素A作为PPAR γ激动剂对LDL颗粒直径和2型糖尿病血脂异常的影响

International Journal of Medical Laboratory Pub Date : 2019-06-01 DOI: 10.18502/IJML.V6I2.1028

D. Ghadimi, M. Goodarzi, M. Bahmani, Z. Khajehahmadi

{"title":"The Effect of Biochanin A as PPAR γ agonist on LDL Particles Diameter and Type 2 Diabetic Dyslipidemia","authors":"D. Ghadimi, M. Goodarzi, M. Bahmani, Z. Khajehahmadi","doi":"10.18502/IJML.V6I2.1028","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1028","url":null,"abstract":"Background and Aims: Small dense low-density lipoproteins (sd-LDL) particles are smaller and heavier than typical LDL ones. They can penetrate into the endothelium of coronary arteries more easily because of their small size. Diabetes mellitus is accompanied by dyslipidemia such as increasing concentration of plasma very low density lipoprotein and sd-LDL. Peroxisome proliferator activated receptor γ (PPARγ ) can decrease the level of sd-LDL in plasma. Biochanin A (BCA), a natural compound, is a PPARγ agonist. The present study was designed to investigate the effect of BCA on sd-LDL-Clolesterol level in diabetic animals. \u0000Materials and Methods: Adult male rats (Wistar strain) were used as the animal models in this study. Animals were made diabetic by single intraperitoneal injection of Streptozotocin- Nicotinamide and then treated by 1 and 5 mg/kg of BCA for 28 days. Body weight and fasting blood glucose were also tested before and at the end of treatment. Furthermore, the size of LDL particles were measured by nondenaturing polyacrylamide gradient gel electrophoresis assay. \u0000Results: Results of the present study indicated that BCA administration at dose of 5mg/kg decreased fasting blood glucose level and increased body weight and diameter of LDL particles in diabetic animals significantly. \u0000Conclusions: BCA seems to be an appropriate agent in diabetes mellitus, because it improves the diabetic dyslipidemia, which is the most important complication in diabetic patients.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"157 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131983993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Molecular Detection of Leptospira Species Serotypes in Iranian Stray Dogs 伊朗流浪犬钩端螺旋体血清型的分子检测

International Journal of Medical Laboratory Pub Date : 2019-05-10 DOI: 10.18502/IJML.V6I2.1032

S. Torkan, H. Momtaz

{"title":"Molecular Detection of Leptospira Species Serotypes in Iranian Stray Dogs","authors":"S. Torkan, H. Momtaz","doi":"10.18502/IJML.V6I2.1032","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1032","url":null,"abstract":"Background and Aims: Leptospirosis is a spirochetal disease with public health importance globally. This disease affects a wide range of domestic and wild animals. Dogs are one of the species most sensitive to Leptospira canicola and Leptospira icterohaemorrhagiae. The present study was concluded to evaluate the prevalence rate of Leptospira species and L. canicola and L. icterohaemorrhagiae serovars in Iranian stray dogs. \u0000Materials and Methods: One-hundred and twenty blood samples were first taken from stray dogs. Then the samples were transferred to the laboratory. Sera were extracted from blood samples and genomic DNA was extracted. DNA samples were subjected to conventional polymerase chain reaction. Positive samples for Leptospira spp. were analyzed for presence of L. canicola and L. icterohaemorrhagiaeserovars using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). \u0000Results: Nine samples out of 120 serum samples (7.5%) were positive for the flagella gene of the Leptospira spp. Prevalence of Leptospira spp. in serum samples of male and female dogs were 5.4% and 10.86%, respectively. Prevalence of L. canicola and L. icterohaemorrhagiae serovars were 55.55% and 33.33%, respectively. We found that 11.11% of samples were positive for both serovars. Two to three and 3-4 year old dogs had the highest prevalence of Leptospira spp. \u0000Conclusions: The considerable prevalence of leptospirta spp. and also their zoonotic serovars among Iranian stray dogs represented an important public health issue regarding the contact of healthy human with these dogs. Identification of infected dogs and their vaccination can inhibit the distribution of Leptospira spp.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128630103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

An Effective Method for Detecting Y-chromosome Specific Sequences of Circulating Fetal DNA in Maternal Plasma During the First-trimester 一种检测妊娠早期母体血浆循环胎儿DNA y染色体特异性序列的有效方法

International Journal of Medical Laboratory Pub Date : 2019-05-10 DOI: 10.18502/IJML.V6I2.1025

N. Davoodian, A. Kadivar, Heidar Heidari Khoie, Sima Hematian Khayat, M. H. Nasirabadi

{"title":"An Effective Method for Detecting Y-chromosome Specific Sequences of Circulating Fetal DNA in Maternal Plasma During the First-trimester","authors":"N. Davoodian, A. Kadivar, Heidar Heidari Khoie, Sima Hematian Khayat, M. H. Nasirabadi","doi":"10.18502/IJML.V6I2.1025","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1025","url":null,"abstract":"Background and Aims: New advances in the use of cell-free fetal DNA (cffDNA) in maternal plasma of pregnant women has provided the possibility of applying cffDNA in prenatal diagnosis as a non-invasive method. One of the applications of prenatal diagnosis is fetal gender determination. Early prenatal determination of fetal sex is required for pregnant women at risk of X-linked and some endocrine diseases. The present study was carried out to perform an efficient polymerase chain reaction (PCR) method in order to improve sensitivity, specificity and accuracy of non-invasive fetal gender detection using fetal DNA in maternal plasma during 8th -12th weeks of pregnancy. \u0000Materials and Methods: Thirty-five pregnant women with 8 to 12 weeks of pregnancy were selected for prenatal fetal sex determination. Maternal peripheral blood was collected and cffDNA was extracted from 3-ml of maternal plasma. Two multi copy Y-chromosome-specific region (DYS and DAZ) and a single copy gene (SRY) were amplified by real-time quantitative PCR. Amplification was labeled as positive, negative, or inconclusive according to a stringent algorithm. \u0000Results: Using this method, the sensitivity and specificity of the real-time PCR assay was 100% and 93.8% for prenatal fetal sex detection, respectively. \u0000Conclusions: It is concluded that fetal sex can be determined with a high level of accuracy by our algorithm, after 8 weeks of gestation with cffDNA analysis.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124355246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Prevalence of Common Mutations in Thrombophilic Patients in Iranian Population with Recurrent Miscarriage 伊朗复发性流产人群中嗜血栓患者常见突变的发生率

International Journal of Medical Laboratory Pub Date : 2019-05-10 DOI: 10.18502/IJML.V6I2.1029

Nasrin Pazoki, F. Naseri

{"title":"The Prevalence of Common Mutations in Thrombophilic Patients in Iranian Population with Recurrent Miscarriage","authors":"Nasrin Pazoki, F. Naseri","doi":"10.18502/IJML.V6I2.1029","DOIUrl":"https://doi.org/10.18502/IJML.V6I2.1029","url":null,"abstract":"Background and Aims: To date, several factors have been reported in recurrent miscarriage. Genetic mutations are the most important causative factors in women. Fetal thrombotic vasculopathy is a new described placental alteration with varying degrees of involvement and often associated with adverse prenatal outcomes. The diagnosis is made histologically and so is postnatal, which makes it a challenge in clinical practice. The aim of the present study is investigation of the common mutations in women with recurrent miscarriage. \u0000Materials and Methods: A cross-sectional study was conducted on 100 women with a history of recurrent miscarriage fetus in 2018. In these patients, several genes such as MTHFR, F2, F5 Leiden, PAI1, F13 and FGB were analyzed by sequencing techniques. The most common mutations in these genes were sequenced and analyzed. \u0000Results: According to statistical results obtained, MTHFR gene (C677T, A1298C) has the highest rate (50 %) of common mutations (p=0.001). After that F2 (G20210A) and F5 Leiden (G1691A) have the highest statistical values (each one 20%). In addition to these genes, there are other unknown mutations which have not been studied in terms of pathogenicity. Other genes have a smaller percentage of aborted fetus infrequently. \u0000Conclusions: Common polymorphisms in the thrombophilic system are likely to result in abortion in these subjects, due to impaired coagulation of the mother and the fetus. Investigating the presence of common mutations and examining their association with other mutations in the thrombophilia as a prognostic in patients with recurrent miscarriage is necessary","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133910390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Therapeutic Applications of Monoclonal Antibodies in Multiple Sclerosis 单克隆抗体在多发性硬化治疗中的应用

International Journal of Medical Laboratory Pub Date : 2019-02-10 DOI: 10.18502/IJML.V6I1.502

H. Musavi, Hemen Moradi-sardareh, M. Mousavi, S. Aslani, Amirhooman Asadi, M. Abbasi

{"title":"Therapeutic Applications of Monoclonal Antibodies in Multiple Sclerosis","authors":"H. Musavi, Hemen Moradi-sardareh, M. Mousavi, S. Aslani, Amirhooman Asadi, M. Abbasi","doi":"10.18502/IJML.V6I1.502","DOIUrl":"https://doi.org/10.18502/IJML.V6I1.502","url":null,"abstract":"Despite the various therapies available, the use of monoclonal antibodies is a highly specific approach that has only recently been of interest to researchers. The properties of antibodies have led to their use in the treatment of various diseases, including cancer, Alzheimer's disease, diabetes and multiple sclerosis (MS). MS, a chronic inflammatory disease, occurs commonly in young adults. The disease is one of the attractive options for monoclonal antibody therapy because it has no definitive drug for its treatment. Antibodies, by targeting different molecules, have different mechanisms to improve the disease. Treatment with monoclonal antibody has culminated in a clear divergence in paradigm and concentration in MS therapeutics. Application of monoclonal antibody in early inflammatory phases can inhibit or postpone the disability in MS subjects. Ocrelizumab and daclizumab are currently under investigation by late phase III trials, and some other monoclonal antibodies are in the early stages of clinical trials. Monoclonal antibodies are of special structural features (including chimeric, humanized, or fully humanized) as well as specific targets (such as stimulation of signal transduction by binding to receptors, blocking interactions, antibody-dependent cell cytotoxicity, complement-dependent cytotoxicity), thus providing various mechanisms of actions during MS therapy. In the present paper, we reviewed different monoclonal antibodies used in MS, their mechanism of action and theirs target molecules.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127756020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Impact of In vitro Oocyte Maturity on Developmental Potential of Embryos Derived from Controlled Ovarian Stimulation Cycles 体外卵母细胞成熟度对受控卵巢刺激周期胚胎发育潜力的影响

International Journal of Medical Laboratory Pub Date : 2019-02-10 DOI: 10.18502/IJML.V6I1.509

F. Montazeri, A. Foroughmand, S. Kalantar, A. Aflatoonian, Alireza Khalili

{"title":"The Impact of In vitro Oocyte Maturity on Developmental Potential of Embryos Derived from Controlled Ovarian Stimulation Cycles","authors":"F. Montazeri, A. Foroughmand, S. Kalantar, A. Aflatoonian, Alireza Khalili","doi":"10.18502/IJML.V6I1.509","DOIUrl":"https://doi.org/10.18502/IJML.V6I1.509","url":null,"abstract":"Background and Aims: One of the major subjects for improving in vitro fertilization (IVF) outcome is the quantity and quality of retrieved oocytes. In vitro maturation (IVM) provides an opportunity for using immature oocytes routinely discarded in clinics. This study aimed at evaluating the quality of embryos derived from in vivo and rescue in vitro matured oocytes. \u0000Materials and Methods: Totally, 462 immature oocytes as cases and 466 mature (MII) oocytes as controls were included for study of their developmental competence. Oocytes underwent intracytoplasmic sperm injection insemination and then denuded oocytes were microscopically assessed regarding cytoplasmic and nuclear maturity and quality. \u0000Results: The morphological assessments showed fertilization rate of 60.9 and 61.4%, the embryo formation rate of 86.7% and 90.9% and arresting rate of 27.3% and 25.6% for the case and control oocytes, respectively. Evaluating embryo quality in the cleavage stage indicated that 63% of the embryos in the case group and 68% of the embryos in the control group were of good quality. There was no significant difference between fertility rate and arresting rate of oocytes matured in both groups, although the embryo formation rate and the quality of embryos differed significantly. \u0000Conclusions: Our findings suggest that IVM is a valuable and practical option for patients who had to cancel IVF treatment cycles because of severe responses or resistance to routine hormonal therapies or those with low functional ovarian reserve.","PeriodicalId":183358,"journal":{"name":"International Journal of Medical Laboratory","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131808843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0