Media Penelitian dan Pengembangan Kesehatan最新文献

{"title":"Pengetahuan, Sikap, dan Praktik Pemilik Kucing yang Berkunjung ke Puskeswan Kota Cimahi terhadap Kejadian Dermatofitosis pada Kucing Peliharaannya","authors":"Dahlia Yulianti, Okta Wismandanu, I. Afriandi","doi":"10.22435/mpk.v31i3.3397","DOIUrl":"https://doi.org/10.22435/mpk.v31i3.3397","url":null,"abstract":"Dermatophytosis is an infection caused by mold and attacks the superficial layer of the cat’s skins. Mold genus which often infects is Microsporum canis, M. gypseum and Trichophyton mentagrophytes. Dermatophytosis can infect cats and spread to humans because it is zoonotic. Pet owners who have a good knowledge and practice of caring for pets can reduce the risk of zoophilic. This study aimed to determine at the relationship of knowledge, attitudes, and practices of cat owners to the incidence of dermatophytosis. This quantitative study used a control case design with 165 cat owners who visited Puskeswan Cimahi in January to February 2020 as the study sample, which consisted of 33 sample cases groups and 132 sample control groups. The results showed that the practice of cat owners variables regarding to release of pet cats, the practice of cleaning the cat’s place to eat and drink, and the practice of washing hands before contacting cats had a relationship with the incidence of dermatophytosis in their pet cats, while the other variables are not related. Promotive about dermatophytosis paints should be increased. That is for increasing the cat owner’s knowledge about dermatophytosis. Good quality of preventive and curative supports is needed to decrease dermatophytosis incidence and also the risk of getting infected to other cats and humans. \u0000Abstrak \u0000Dermatofitosis merupakan salah satu infeksi yang disebabkan oleh kapang dan menyerang lapisan superfisialis pada kulit kucing. Genus kapang yang paling sering menyerang yaitu Microsporum canis, Microsporum gypseum dan Trichophyton mentagrophytes. Dermatofitosis dapat menyerang kucing serta dapat menular kepada manusia karena bersifat zoofilik. Pemilik hewan yang memiliki pengetahuan dan praktik pemeliharaan hewan yang baik dapat mengurangi risiko terjadinya transmisi infeksi ini. Penelitian ini bertujuan untuk menentukan hubungan pengetahuan, sikap, dan praktik pemilik kucing terhadap kejadian dermatofitosis pada kucing peliharaannya. Penelitian kuantitatif ini menggunakan desain kasus kontrol dengan sampel penelitian sebanyak 165 pemilik kucing yang berkunjung ke Puskeswan Kota Cimahi periode bulan Januari-Februari 2020, sampel penelitian tersebut terdiri dari 33 sampel kelompok kasus dan 132 sampel kelompok kontrol. Hasil penelitian menunjukkan bahwa praktik pemilik kucing mengenai pelepasliaran kucing, praktik membersihkan tempat makan dan minum kucing, dan praktik mencuci tangan sebelum berkontak dengan kucing memiliki hubungan terhadap kejadian dermatofitosis pada kucing peliharaannya, sedangkan variabel lainnya tidak berhubungan. Upaya promotif tentang dermatofitosis pada kucing perlu ditingkatkan, hal ini bertujuan agar pengetahuan pemilik kucing mengenai dermatofitosis dapat meningkat. Dukungan preventif dan kuratif yang berkualitas serta berkelanjutan pun diperlukan agar dapat mengurangi kejadian dermatofitosis dan risiko penularannya kepada sesama kucing maupun manusia.","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84159355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Back Matter Media Penelitian dan Pengembangan Kesehatan Vol. 31 No. 2 Tahun 2021","authors":"S. Lestari","doi":"10.22435/mpk.v31i2.5560","DOIUrl":"https://doi.org/10.22435/mpk.v31i2.5560","url":null,"abstract":"","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78902183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Matter Media Penelitian dan Pengembangan Kesehatan Vol. 31 No. 2 Tahun 2021","authors":"S. Lestari","doi":"10.22435/mpk.v31i2.5559","DOIUrl":"https://doi.org/10.22435/mpk.v31i2.5559","url":null,"abstract":"","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73770648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Keluhan Low Back Pain Selama Pembelajaran Jarak Jauh pada Mahasiswa UIN Syarif Hidayatullah Jakarta Tahun 2020","authors":"Tri Aulia Fitriani, Qonita Nur Salamah, Hoirun Nisa","doi":"10.22435/MPK.V31I2.4180","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.4180","url":null,"abstract":"Low back pain can be one of the complaints for students during distance learning because it allows students to do activities that tend to be in monotonous and periodic positions and are in the wrong sitting position. The purpose of this study is to find out the factors that affect low back pain complaints during distance learning in UIN Syarif Hidayatullah Jakarta students in 2020. This study used a cross-sectional design, with 394 active students as respondents of the study. Data were collected using a questionnaire distributed using the internet. Multivariate analysis was performed by a logistic regression test. LBP complaints occurred in 70.6% students. Multivariate analysis indicated that students with duration of lecture time more than 5 hours per day (OR=1.81; 95% CI=1.07-3.06)and students with non-ergonomic position during lecture time (OR=2.35; 95% CI=1.45-3.81) were associated with LBP complaints. Meanwhile, duration of gadget using (OR=1.29; 95% CI=0.81 - 2.07) was not associated with LBP complaints. The conclusion in this study is that duration of lecture and body position when attending lecture were related to LBP complaints in students during distance learning. The results of this study provide recommendations to the lecturers of UIN Syarif Hidayatullah Jakarta to encourage students to sit in an ergonomic position during online-class to prevent LBP complaints. \u0000Abstrak \u0000  \u0000Low back pain (LBP) dapat menjadi salah satu keluhan bagi mahasiswa selama pembelajaran jarak jauh dikarenakan mahasiswa melakukan aktivitas yang cenderung berada pada posisi monoton dan berkala serta berada pada posisi duduk yang salah. Tujuan penelitian ini adalah untuk mengetahui faktor-faktor yang berhubungan dengan keluhanLBP selama pembelajaran jarak jauh pada mahasiswa UIN Syarif Hidayatullah Jakarta tahun 2020. Desain studi penelitian ini menggunakan desain studi cross-sectional, dengan jumlah total responden yaitu 394 mahasiswa aktif. Pengumpulan data dilakukan dengan menggunakan kuesioner yang dibagikan melalui internet. Analisis multivariat dilakukan dengan menggunakan uji regresi logistik. Hasil penelitian menunjukkan sebanyak 278 (70,6%) mahasiswa mengalami keluhan LBP. Adapun hasil analisis multivariat menunjukkan bahwa mahasiswa dengan lama waktu kuliah >5 jam/hari (OR=1,81; 95% CI=1,07-3,06) dan mahasiswa dengan posisi tubuh tidak ergonomis ketika perkuliahan (OR=2,35; 95% CI=1,45-3,81) berhubungan dengan keluhan LBP selama pembelajaran jarak jauh. Sementara itu, lama menggunakan gadget (OR=1,29; 95% CI=0,81-2,07)) tidak berhubungan dengan kejadian keluhan LBP. Simpulan dalam penelitian ini adalah faktor lama waktu kuliah dan posisi tubuh ketika perkuliahan berhubungan dengan keluhan LBP pada mahasiswa selama pembelajaran jarak jauh. Hasil penelitian ini memberikan rekomendasi kepada para pengajar UIN Syarif Hidayatullah Jakarta untuk menghimbau mahasiswa duduk dengan posisi ergonomis ketika kuliah daring untuk mencegah keluhan LBP.","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86506699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Faktor Risiko Obesitas, Jenis Kelamin, dan Merokok pada Pasien Artritis Reumatoid terhadap Kejadian Hipertensi","authors":"Shiany Henly Citraminata, Ika Vemilia Warlisti, Andreas Arie Setiawan, Aryu Candra","doi":"10.22435/MPK.V31I2.4006","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.4006","url":null,"abstract":"Cardiovascular disease is one of the comorbid in rheumatoid arthritis (RA), which significantly affects the morbidity and mortality of patients with RA. Hypertension is a manifestation of progressive cardiovascular symptoms, which caused by endothelial dysfunction due to continuous inflammation in RA patients. The risk factors of hypertension in RA are obesity, gender, smoking, and consumption of an antihypertensive and antirheumatic drug (NSAIDs, glucocorticoids, and leflunomide). This research utilized a cross-sectional study to analyze the risk factors of obesity, gender, and smoking on hypertension in RA patients at RSUP Dr. Kariadi Semarang. The weigh, height, and blood pressure  of 24 RA patients (consist of 12 men and 12 women) who had been diagnosed with RA for at least one year were measured using the weight and height scales of GEA SMIC ZT-120 (with an accuracy of 0.1kg and 0.1cm) and mercury sphygmomanometer Riester Nova Ecoline (with an accuracy of 2 mmHg) respectively. The result showed that male RA patients (p = 0.041) were risk factors for hypertension. RA patients with smoking and obesity had a risk factor of 1.4 times (p = 0.043, OR = 1.395) and 1.9 times (p = 0.012, OR = 1.882) on the incidence of hypertension respectively \u0000Abstrak \u0000Penyakit kardiovaskular merupakan salah satu komorbiditas Artritis Reumatoid (AR) yang berpengaruh signifikan terhadap peningkatan morbiditas dan mortalitas pasien AR. Hipertensi merupakan manifestasi dari kumpulan gejala kardiovaskular yang progresif. Hipertensi pada pasien AR disebabkan karena adanya disfungsi endotel akibat proses inflamasi yang berlangsung terus menerus. Beberapa faktor risiko  hipertensi pada pasien AR yaitu, obesitas, jenis kelamin, kebiasaan merokok, konsumsi obat anti hipertensi, dan obat AR (obat antiinflamasi nonsteroid (OAINS), glukokortikoid, dan leflunomide). Penelitian ini merupakan studi belah lintang, bertujuan menganalisis faktor risiko obesitas, jenis kelamin, dan merokok pada pasien AR terhadap kejadian hipertensi di RSUP Dr. Kariadi Semarang. Dua puluh empat (24) pasien AR (terdiri dari 12 pria dan 12 wanita) yang sudah terdiagnosis AR selama minimal 1 tahun dilakukan pengukuran tinggi badan dan berat badan (menggunakan timbangan berat badan dan tinggi badan merk GEA SMIC ZT-120 dengan ketelitian 0,1 kg dan 0,1 cm, dan tekanan darah (menggunakan tensimeter air raksa merk Riester Nova Ecoline dengan ketelitian 2 mmHg). Hasil penelitian menunjukan bahwa pasien AR pria (p  = 0,041) merupakan faktor risiko terjadinya hipertensi. Pasien AR yang merokok dan obesitas secara berurutan berisiko 1,4 kali (p = 0,043, OR = 1,395) dan 1,9 kali (p = 0,012, OR = 1,882) terhadap kejadian hipertensi.","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82615705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studi pada Wali Murid di Kota Magelang Provinsi Jawa Tengah: Faktor yang Berhubungan dengan Penolakan Imunisasi Measles Rubella","authors":"Zulfa Fatmawati Dwi Asdika, Martini Martini, Dwi Sutiningsih, L. Saraswati","doi":"10.22435/mpk.v31i2.3183","DOIUrl":"https://doi.org/10.22435/mpk.v31i2.3183","url":null,"abstract":"Measles rubella (MR) is a highly contagious disease, rubella causes miscarriage or baby-ies born with congenital defects in the first trimester of pregnancy. The Ministry of Health recommends 95% of the population against rubella measles through the Measles Rubella Immunization Program. However, there is still MR immunization coverage below 95%, in the Magelang City. Public Health Center has coverage of MR was 83.56% and Magelang Selatan was 89.14%. The purpose of the study was to analyse the factors associated with rejection of MR immunization. Study design using cross sectional. 120 samples of parents from elementary, junior high school, and SLB were selected in Magelang Utara sub-district with simple random sampling. The variables that had a statistical- relationship with the status of immunization refusal of Measles Rubella (p < 0.05) were type of work, history of measles,level of knowledge, attitudes of student’s parent attitudes (p = 0,0001), perceived vulnerability (p = 0,0001), perceived severity (p = 0,0001), perceived benefits and barriers, environment support, family support, and school support. So that parents with poor knowledge have a 18.355 times greater chance of rejecting Measles rubella Immunization. \u0000Abstrak \u0000Measles rubella (MR) merupakan penyakit yang sangat menular, rubela mengakibatkan keguguran atau bayi lahir dengan cacat bawaan pada kehamilan trimester I. Kementerian Kesehatan menganjurkan kekebalan populasi terhadap penyakit campak rubela sebesar 95% melalui Program Imunisasi Measles Rubella. Cakupan MR di Kota Magelang Provinsi Jawa Tengah masih < 95%.  Puskesmas Magelang Utara mempunyai cakupan MR sebesar 83,56%, dan  Magelang Selatan sebesar 89,14%. Penelitian ini bertujuan untuk menganalisis faktor-faktor yang berhubungan dengan penolakan imunisasi MR. Desain studi menggunakan potong lintang. Terpilih 120 sampel wali murid dari SD, SMP, dan SLB di Kecamatan Magelang Utara dengan simple random sampling. Variabel yang memiliki hubungan secara statistik dengan status penolakan imunisasi Measles Rubella adalah jenis pekerjaan, riwayat campak, tingkat pengetahuan, sikap wali murid (p=0,0001), persepsi kerentanan (p=0,0001), persepsi keparahan (p=0,0001), persepsi manfaat dan hambatan, dukungan lingkungan, dukungan keluarga, dan dukungan sekolah. Wali murid dengan pengetahuan kurang baik memiliki peluang 18,355 kali lebih besar untuk menolak Imunisasi MR. \u0000 ","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83549064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analisis Pelaksanaan Timbang Terima Pasien Antar Perawat di Unit Rawat Inap RSU “X” Tahun 2019","authors":"Hendra Setiawan, Nikmah Fitriasari","doi":"10.22435/MPK.V31I2.3736","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.3736","url":null,"abstract":"Inpatient care is a patient safety centered care. Inter-shift handover is a critical time that needs special attention to maintain and improve patient safety. This study aims to identify problems in nurse shifts handover implementation in inpatient unit and determine solutions to overcome those problems. This research is a social qualitative research using focus group discussion (FGD) method preceded by secondary data identification. The research was conducted from August to October of 2019 in one of the adult inpatient units of class C hospitals in Malang, East Java. The informants involved in this study were twelve executive nurses and three nursing management who were divided into two different discussion groups. The chosen solutios are  determine the needs to make policy draft that define communication methods, effective communication guidelines that include monitoring and evaluation obligations as well as SOPs for implementation of inter-shift handover as well as compiling proposals for socialization, training and workshops. The conclusion of this study shows that inter-shift handover implementation at the hospital “X” has problems related to internal regulation and understanding of human resources for health workers on their professional duties. The process of identifying problems and determining solutions by involving stakeholders will be able to reflect conditions that are closer to reality in daily implementation. \u0000Abstrak \u0000Layanan rawat inap adalah pelayanan kesehatan berpusat pada keselamatan pasien. Timbang terima adalah salah satu waktu kritis yang perlu mendapat perhatian khusus untuk menjaga dan meningkatkan keselamatan pasien. Penelitian ini bertujuan untuk mengidentifikasi masalah dalam pelaksanaan timbang terima antar shift perawat pada unit rawat inap dan menentukan solusi untuk mengatasi masalah tersebut. Penelitian kualitatif sosial ini dilaksanakan menggunakan metode diskusi terarah yang didahului dengan dengan identifikasi data sekunder. Penelitian dilaksanakan pada bulan Agustus hingga Oktober tahun 2019 di salah satu unit rawat inap dewasa rumah sakit umum kelas C di daerah Malang, Jawa Timur. Partisipan yang terlibat dalam penelitian ini berjumlah dua belas orang perawat pelaksana dan tiga orang pihak manajemen bidang keperawatan. Hasil yang diperoleh yaitu belum ada kebijakan yang menetapkan metode komunikasi, belum ada usulan sosialisasi dari Bidang Keperawatan, belum ada kebijakan yang menetapkan metode monitoring dan evaluasi komunikasi timbang terima yang menyebabkan belum adanya metode komunikasi yang dibakukan dan panduan pelaksanaan yang mengatur tentang bagaimana komunikasi efektif tersebut dilaksanakan, serta kurangnya advokasi peran perawat. Pada tahap penentuan solusi didapatkan penyusunan draf kebijakan yang menetapkan metode komunikasi, panduan komunikasi efektif yang menyertakan kewajiban monitoring dan evaluasi serta SOP pelaksanaan timbang terima disertai dengan menyusun usulan sosialisasi, pelatihan, da","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77940912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengetahuan, Sikap, dan Perilaku Pencegahan COVID-19 Mahasiswa","authors":"Hasna Linawati, Salma Nur Helmina, Virliana Aulia Intan, Wanda Septi Oktavia, Hany Fauzia Rahmah, Hoirun Nisa","doi":"10.22435/MPK.V31I2.3456","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.3456","url":null,"abstract":"Corona Virus Disease-19 (COVID-19) is the virus that causes an epidemic of acute respiratory infections that become a global pandemic in 2020. COVID-19 cases continue to increase, so prevention behavior is needed. Knowledge and attitudes become important domains that can influence a person's behavior. This study aims to determine the relationship between knowledge and attitudes related to COVID-19 prevention behaviors in university students. This study used a cross sectional study design which was conducted from April to May 2020. Students from the faculty of religion at UIN Syarif Hidayatullah Jakarta (n = 434) were participated in this study. As many as 51.4% (n = 223) of participants had a positive attitude towards preventing COVID-19. Participants who are well-behaved attitude towards COVID-19 prevention tend to have moderate and high levels of knowledge about COVID-19 and positive attitude (59.7%) towards COVID-19 prevention. The multivariate results showed that levels of knowledge and attitudes were related to COVID-19 prevention behaviors with adjustment for age, sex, and place of residence (p-value < 0.05). We conclude that knowledge and attitudes have an influence on a person's behavior in preventing the transmission of COVID-19. \u0000Abstrak \u0000Corona Virus Disease-19 (COVID-19) adalah virus penyebab wabah infeksi pernapasan akut yang menjadi pandemi global pada tahun 2020. Kasus COVID-19 terus mengalami peningkatan, sehingga diperlukan adanya perilaku pencegahan. Pengetahuan dan sikap menjadi domain penting yang dapat memengaruhi perilaku seseorang. Penelitian ini bertujuan untuk mengetahui hubungan pengetahuan dan sikap terkait COVID-19 dengan perilaku pencegahan COVID-19 pada mahasiswa. Studi ini menggunakan desain studi cross sectional yang dilakukan pada April–Mei 2020. Mahasiswa dari fakultas keagamaan UIN Syarif Hidayatullah Jakarta (n=434) adalah partisipan dalam penelitian ini. Sebanyak 51,4% (n=223) partisipan berperilaku positif terhadap pencegahan COVID-19. Partisipan yang berperilaku baik terhadap pencegahan COVID-19 cenderung memiliki tingkat pengetahuan yang sedang dan tinggi tentang COVID-19 dan sikap positif (59,7%) terhadap pencegahan COVID-19. Hasil multivariat menunjukkan bahwa tingkat pengetahuan dan sikap berhubungan dengan perilaku pencegahan COVID-19 dengan penyesuaian variabel usia, jenis kelamin, dan tempat tinggal (nilai p-value < 0,05). Kami menyimpulkan bahwa pengetahuan dan sikap memiliki pengaruh pada perilaku seseorang dalam mencegah COVID-19.","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77558839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Konstruksi Plasmid Pengekspresi Antigen Gag dan Protein Penghantar VP22 untuk Pengembangan Vaksin HIV-1","authors":"Melinda Remelia, Budiman Bela, Silvia Tri Widyaningtyas, Fera Ibrahim","doi":"10.22435/MPK.V31I2.3046","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.3046","url":null,"abstract":"The endogenous HIV-1 vaccine based on Gag protein is expected to stimulate the immune response of CD8+ T cells (cytotoxic). The Gag protein that has been produced by the E.coli prokaryote system is an exogenous antigen. The fusion of VP22 protein is expected to deliver Gag antigen into the cytoplasm of cell, observed by eGFP markers. Sequences of VP22 (114 pb), GagHIV-1 (1506 pb), and eGFP (733 pb) were inserted into the pQE80L, respectively. The recombinant protein was expressed in the E.coli system and purified by the Ni-NTA method. Antigen delivery fused with VP22 and eGFP was observed with fluorescence and confocal microscopy. The recombinant plasmid constructs of protein expression eGFP, VP22-eGFP, GagHIV-1-eGFP, VP22-GagHIV-1-eGFP were verified by DNA sequencing according to the reference. The recombinant plasmid constructs of Gag HIV-1-eGFP and VP22-GagHIV-1-eGFP still need to be optimized so they can be expressed in the E.coli system.  The recombinant protein VP22-eGFP (27.02 kDa) was succesfully obtained and fluorescent green (entered) into the cytoplasm and nucleus of vero cells. In addition to the HIV-1 vaccine, this recombinant plasmids pQE80L-eGFP and pQE80L-VP22-eGFP also have the potential to be used as tools in the development of endogenous vaccines for another viruses/microbes. \u0000Abstrak \u0000Vaksin endogen HIV-1 berbasis protein Gag diharapkan dapat menstimulus respons imun sel T CD8+ (sitotoksik). Protein Gag yang telah diproduksi dengan sistem prokariota E.coli merupakan antigen yang bersifat eksogen. Fusi protein VP22 diharapkan mampumenghantarkan antigen Gag masuk ke sitoplasma sel, diamati dengan marker eGFP.  Sekuens VP22 (114 pb), GagHIV1 (1506 pb), dan eGFP (733 pb) telah diinsersikan pada vektor pQE80L. Protein rekombinan diekspresikan pada sistem E.coli dan dipurifikasi dengan metode Ni-NTA. Penghantaran antigen yang difusikan dengan VP22 dan marker eGFP diamati dengan mikroskop fluoresens dan konfokal. Konstruksi plasmid rekombinan pengekspresi protein eGFP, VP22-eGFP, GagHIV1-eGFP, dan VP22-GagHIV1-eGFP telah diverifikasi dengan sekuensing DNA sesuai dengan sekuen referensi. Plasmid rekombinan pengekspresi GagHIV1-eGFP dan VP22-GagHIV1-eGFP masih perlu dioptimasi agar dapat diekspresikan di sistem E.coli. Protein rekombinan VP22-eGFP (27,02 kDa) telah berhasil diperoleh serta berpendar fluoresens hijau  (masuk) ke sitoplasma dan nukleus sel vero. Selain vaksin HIV-1, plasmid rekombinan pQE80L-eGFP dan pQE80L-VP22-eGFP juga berpotensi dapat digunakan sebagai ‘tools’ dalam pengembangan vaksin endogen dari virus atau mikroba lainnya.","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75819898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifikasi Jamur Beracun Clitocybe sp. di Gresik, Indonesia (Studi Kasus)","authors":"Ivan Permana Putra, R. Hermawan","doi":"10.22435/MPK.V31I2.4352","DOIUrl":"https://doi.org/10.22435/MPK.V31I2.4352","url":null,"abstract":"Wild mushrooms are one of the food materials which often sought after by Indonesians when foraging on forests or around their residence. However, due to the similar morphology of edible and poisonous mushrooms, cases of mushroom poisoning are an inevitable risk. A young man consumed wild mushroom collected from his yard in Gresik, East Java. The macrofungi grew on humus soil with several plants around it. The man mistakenly recognized the mushroom as either Termitomyces or Agaricus which could be consumed. After an experienced friend also confirmed the edibility of the mushroom, he immediately cooked and consumed the mushrooms. A few hours later, the victim experienced vomiting, nausea, dizziness, and loss of balance. The victim immediately received coconut water by his family. After 4-5 hours of seven times vomiting, his condition began to improve and start the norma activities. The macrofungi which caused poisoning were then documented and described. The identification result using several macroscopic characters confirmed the mushroom as Clitocybe sp. This mushroom has previously been reported contain the muscarine toxin. This article is one of few publications on mushroom poisoning case which equipped with the complete information on mushroom features and descriptions in Indonesia. This report indicates that the caution and complete observations of the information are needed before consuming an unknown wild mushroom. \u0000Abstrak \u0000 \u0000Jamur liar merupakan salah satu bahan pangan yang sering dicari oleh masyarakat Indonesia ketika merambah di hutan ataupun sekitar tempat tinggalnya. Namun, karena morfologi jamur yang bisa dikonsumsi dan jamur beracun seringkali terlihat mirip satu sama lain, maka kasus keracunan jamur menjadi risiko yang tidak terhindarkan. Seorang pemuda mengonsumsi jamur liar yang dikoleksi dari pekarangan rumahnya di Gresik, Jawa Timur. Jamur tersebut tumbuh pada tanah berhumus dengan beberapa tanaman di sekitarnya. Korban salah mengenali jamur tersebut sebagai Termitomyces ataupun Agaricus yang bisa dikonsumsi. Setelah seorang teman juga mengkonfirmasi edibilitas dari jamur tersebut, korban segera memasak dan mengkonsumsi jamur yang ditemukan. Beberapa jam kemudian korban mengalami muntah-muntah, mual, pusing, dan hilang keseimbangan. Korban segera mendapatkan pertolongan pertama berupa air kelapa oleh keluarganya. Setelah 4-5 jam mengalami muntah sebanyak tujuh kali, kondisi korban mulai membaik dan bisa kembali beraktifitas normal. Jamur yang menyebabkan keracunan kemudian didokumentasikan dan dibuatkan pertelaan karakter basidiomata. Hasil identifikasi dengan pendekatan beberapa karakter makroskopik mengkonfirmasi jamur tersebut sebagai Clitocybe sp. Jamur ini sebelumnya telah dilaporkan memiliki kandungan racun muscarine. Tulisan ini merupakan salah satu dari sedikit publikasi keracunan jamur yang dilengkapi dengan informasi jamur lengkap dan dilengkapi deskripsinya di Indonesia. Studi ini mengindikasikan bahwa diperlukan keh","PeriodicalId":18323,"journal":{"name":"Media Penelitian dan Pengembangan Kesehatan","volume":null,"pages":null},"PeriodicalIF":0.1,"publicationDate":"2021-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88797961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}