Marine genomics最新文献

{"title":"Bioactivity of bacteria associated with Red Sea nudibranchs and whole genome sequence of Nocardiopsis dassonvillei RACA-4","authors":"Hossam H. Elfeky ,&nbsp;Amro Hanora ,&nbsp;Samar M. Solyman","doi":"10.1016/j.margen.2022.101004","DOIUrl":"10.1016/j.margen.2022.101004","url":null,"abstract":"<div><p>Microorganisms associated with marine invertebrates consider an important source of bioactive products. This study aimed to screen for antimicrobial and anticancer activity of crude extracts of bacteria associated with Red sea nudibranchs and molecular identification of the bioactive isolates using 16Sr RNA sequencing, in addition to whole-genome sequencing of one of the most bioactive bacteria. This study showed that bacteria associated with Red sea nudibranchs are highly bioactive and 16Sr RNA sequencing results showed that two isolates belonged to Firmicutes, and two isolates belonged to Proteobacteria, and Actinobacteria. The whole genome sequence data of the isolated <strong><em>Nocardiopsis RACA4</em></strong> isolate has an estimated genome length of 6,721,839 bp and the taxonomy showed it belongs to the bacteria <em>Nocardiopsis dassonvillei</em>. The De novo assembly of RACA-4 paired reads using Unicycler v0.4.8 initially yielded 97 contigs with an N50 value of 214,568 bp and L50 value of 10, The resulting assembly was further mapped to the reference genome <em>Nocardiopsis dassonvillei</em> strain NCTC10488 using RagTag software v.2.1.0 and a final genome assembly resulted in 39 contigs and N50 value of 6,726,007 and L50 of 1. Genome mining using anti-smash showed around 9.1% of the genome occupied with genes related to secondary metabolites biosynthesis. A wide variety of secondary metabolites belonging to Polyketides, Terpenes, and nonribosomal peptides were predicted with high degree of similarity to known compounds. Non-characterized clusters were also found which suggest new natural compounds discovered by further studies.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"67 ","pages":"Article 101004"},"PeriodicalIF":1.9,"publicationDate":"2023-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10627920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo assembly and annotation of the transcriptome of the endangered seagrass Zostera capensis: Insights from differential gene expression under thermal stress","authors":"Andrew Ndhlovu,&nbsp;Sophie von der Heyden","doi":"10.1016/j.margen.2022.100984","DOIUrl":"10.1016/j.margen.2022.100984","url":null,"abstract":"<div><p><span>Seagrasses are important marine ecosystem engineers but anthropogenic impacts and climate change have led to numerous population declines globally. In South Africa, </span><span><em>Zostera</em><em> capensis</em></span><span> is endangered due to fragmented populations and heavy anthropogenic pressures on estuarine ecosystems that house the core of the populations. Addressing questions of how pressures such as climate change affect foundational species, including </span><em>Z. capensis</em><span> are crucial to supporting their conservation and underpin restoration efforts. Here we use ecological transcriptomics to study key functional responses of </span><em>Z. capensis</em><span> through quantification of gene expression after thermal stress and present the first reference transcriptome of </span><em>Z. capensis</em>. Four <em>de novo</em><span> reference assemblies (Trinity, IDBA-tran, RNAspades, SOAPdenovo) filtered through the EvidentialGene pipeline resulted in 153,755 transcripts with a BUSCO score of 66.1% for completeness. Differential expression analysis between heat stressed (32 °C for three days) and pre-warming plants identified genes involved in photosynthesis<span>, oxidative stress, translation, metabolic and biosynthetic processes in the </span></span><em>Z. capensis</em> thermal stress response. This reference transcriptome is a significant contribution to the limited available genomic resources for <em>Z. capensis</em> and represents a vital tool for addressing questions around the species restoration and potential functional responses to warming marine environments.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"66 ","pages":"Article 100984"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40363071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of piezotolerant Stutzerimonas kunmingensis 7850S isolated from the sediment of the Mariana Trench","authors":"Jia-Wei Wang ,&nbsp;Kun Shang ,&nbsp;Sheng-Yi Wu ,&nbsp;Bo-Yu Zhu ,&nbsp;Mei-Gui Han ,&nbsp;Yuan Zhao ,&nbsp;Yi-Qiu Chen ,&nbsp;Jia Xie ,&nbsp;Bo Huang ,&nbsp;Zhuang Han","doi":"10.1016/j.margen.2022.100996","DOIUrl":"10.1016/j.margen.2022.100996","url":null,"abstract":"<div><p><em>Stutzerimonas kunmingensis</em> 7850S is a piezotolerant bacterium isolated from the sediment of the Mariana trench. Here, we described the complete genome of strain 7850S, which contains a single circular chromosome of 4,775,870 base pairs with 62.66% G + C content, and harbors 4494 protein-coding genes, 65 transfer RNA genes, and 12 ribosomal RNA genes. The experimental results showed that strain 7850S could grow under hydrostatic pressure of 0.1–70 MPa. Genomic analyses led to identifications of numbers of high hydrostatic pressure-associated genes, such as the ones associated with unsaturated fatty acids, betaine, and ectoine. A complete set of denitrification genes and some heavy metal detoxification genes were also found in this strain. The presence of these genes suggests metabolic characteristics for adaption to hadal environments and provides insights to further understand adaption strategies and ecological roles of <em>Stutzerimonas</em> in hadal environments.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"66 ","pages":"Article 100996"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40696158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence of the denitrifying Pseudomonas sp. strain DNDY-54 isolated from deep-sea sediment of ninety east ridge","authors":"Luying Zhao ,&nbsp;Yuanyuan Gui ,&nbsp;Ao Zhang ,&nbsp;Qian Zhang ,&nbsp;Liping Fu ,&nbsp;Jiang Li","doi":"10.1016/j.margen.2022.100995","DOIUrl":"10.1016/j.margen.2022.100995","url":null,"abstract":"<div><p><em>Pseudomonas</em> sp. strain DNDY-54, a denitrifying bacterium, was isolated from a deep-sea sediment sample from Ninety East Ridge in the Indian Ocean. Here, we show that the complete genome of DNDY-54 has one circular chromosome of 4,412,895 bp with mean 60.57% GC content. The complete genome contains 4111 predicted protein-coding genes, 59 tRNAs, and 4 rRNA operons as 16S–23S-5S rRNA. On the basis of the annotation results, we identified genes that encode 27 proteins related to nitrogen metabolism, including enzymes that make up a complete denitrifying pathway. This work will improve the understanding of nitrogen cycling in the deep biosphere and provides a new candidate for protection of the environment and applications in waste water disposal.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"66 ","pages":"Article 100995"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40712864","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial diversity associated with the excavating sponge Thoosa mismalolli from East Pacific Coral reefs","authors":"Adrián González-Castillo ,&nbsp;José Luis Carballo ,&nbsp;Eric Bautista-Guerrero","doi":"10.1016/j.margen.2022.100994","DOIUrl":"10.1016/j.margen.2022.100994","url":null,"abstract":"<div><p><em>Thoosa mismalolli</em><span><span> is one of the most abundant and common excavating sponges along Mexican and Central America shallow reefs. This sponge harbors a </span>microbiome<span> unknown so far. In the present study, the metagenome of </span></span><em>T. mismalolli</em><span> was sequenced, and total reads obtained were classified, and contigs<span> were assembled to analyze the microbiota. The results showed that the highest number of contigs was assigned to the phylum ‘</span></span><em>Candidatus</em><span> Poribacteria’ (8848), followed by the phylum Proteobacteria (6415), and Chloroflexi (3972). 22 MAGs with &lt;8.5% redundancy using MaxBin 2 were detected: ‘</span><em>Candidatus</em><span><span> Poribacteria’ (5), Proteobacteria (5), Chloroflexi (6), Gemmatimonadetes<span> (2), Actinobacteria (2), and </span></span>Thaumarchaeota<span><span> (2). The phylogenetic tree based on the 139 single-copy gene (SCG) suggested a subdivision into two clades of the phylum Proteobacteria. The presence Thaumarchaeota is also interesting to highlight because contains ammonia-oxidizing </span>archaea<span><span><span><span> considered key members of the global nitrogen and carbon biogeochemical cycles. In addition, shotgun </span>metagenomic analysis revealed that </span>protein sequences associated for </span>amino acids<span> (13.0%) and carbohydrate metabolism (12.4%) predominated. In this study, the first microbiome and functional potential of </span></span></span></span><em>T. mismalolli</em> is reported, which also represents the first investigation of a microbiota sponge in the Mexican Pacific reefs.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"66 ","pages":"Article 100994"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40559680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bimodal distribution of seafloor microbiota diversity and function are associated with marine aquaculture","authors":"R. Pettersen ,&nbsp;I. Ormaasen ,&nbsp;I.L. Angell ,&nbsp;N.B. Keeley ,&nbsp;A. Lindseth ,&nbsp;L. Snipen ,&nbsp;K. Rudi","doi":"10.1016/j.margen.2022.100991","DOIUrl":"10.1016/j.margen.2022.100991","url":null,"abstract":"<div><p><span><span><span>The aim of the current work was to investigate the impact of marine aquaculture on seafloor </span>biogeochemistry and diversity from pristine environments in the northern part of Norway. Our analytical approach included analyses of 182 samples from 16 aquaculture sites using 16S and 18S rRNA, shotgun analyses, visual examination of macro-organisms, in addition to chemical measurements. We observed a clear bimodal distribution of the </span>prokaryote<span> composition and richness, determined by analyses of 16S rRNA gene<span> operational taxonomic units<span><span> (OTUs). The high OTU richness cluster was associated with non-perturbed environments and farness from the aquaculture sites, while the low OTU richness cluster was associated with perturbed environments and proximity to the aquaculture sites. Similar patterns were also observed for eukaryotes using 18S rRNA gene analyses and visual examination, but without a bimodal distribution of OTU richness. Shotgun sequencing showed the </span>archaeum </span></span></span></span><em>Nitrosopumilus</em> as dominant for the high OTU richness cluster, and the epsilon protobacterium <em>Sulfurovum</em> as dominant for the low OTU richness cluster. Metabolic reconstruction of <em>Nitrosopumilus</em> indicates nitrification as the main metabolic pathway. S<em>ulfurovum</em><span><span>, on the other hand, was associated with sulfur oxidation and denitrification. Changes in nitrogen and </span>sulfur metabolism<span> is proposed as a potential explanation for the difference between the high and low OTU richness clusters. In conclusion, these findings suggest that pollution from elevated loads of organic waste drives the microbiota towards a complete alteration of respiratory routes and species composition, in addition to a collapse in prokaryote OTU richness.</span></span></p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"66 ","pages":"Article 100991"},"PeriodicalIF":1.9,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40363070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome profiling of the early developmental stages in the giant mussel Choromytilus chorus exposed to delousing drugs","authors":"Gustavo Núñez-Acuña ,&nbsp;Camila Fernandez ,&nbsp;Sandra Sanhueza-Guevara ,&nbsp;Cristian Gallardo-Escárate","doi":"10.1016/j.margen.2022.100970","DOIUrl":"10.1016/j.margen.2022.100970","url":null,"abstract":"<div><p>The giant mussel <em>Choromytilus chorus</em><span> is a marine bivalve commonly collected in central – southern Chile from fishery zones shared with the salmon industry. These economically relevant areas are also affected by the use of pesticides for controlling sea lice infestations in salmon aquaculture. Their main target is the sea louse </span><em>Caligus rogercresseyi.</em> However, other than some physiological impacts, the molecular effects of delousing drugs in non-target species such as <em>C. chorus</em><span><span> remain largely understudied. This study aimed to explore the transcriptome modulation of </span>Trochophore and D larvae stages of </span><em>C. chorus</em><span> after exposure to azamethiphos and deltamethrin<span><span> drugs. Herein, RNA-seq analyses and mRNA-lncRNAs molecular interactions were obtained. The most significant changes were found between different </span>larval development<span><span> stages exposed to delousing drugs. Notably, significant transcriptional variations were correlated with the drug concentrations tested. The biological processes involved in the development, such as cell movement and transcriptional activity, were mainly affected. Long non-coding RNAs (lncRNAs) were also identified in this species, and the transcription activity showed similar patterns with coding mRNAs. Most of the significantly expressed </span>lncRNAs<span> were associated with genes annotated to matrix metalloproteinases<span>, collagenases, and transcription factors. This study suggests that exposure to azamethiphos or deltamethrin drugs can modulate the transcriptome signatures related to the early development of the giant mussel </span></span></span></span></span><em>C. chorus</em>.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"65 ","pages":"Article 100970"},"PeriodicalIF":1.9,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40508453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequence and Carbohydrate Active Enzymes (CAZymes) repertoire of Gilvimarinus sp. DA14 isolated from the South China Sea","authors":"Qun-Jian Yin ,&nbsp;Hong-Zhi Tang ,&nbsp;Fang-Chao Zhu ,&nbsp;Xu-Yang Chen ,&nbsp;De-Wei Cheng ,&nbsp;Li-Chang Tang ,&nbsp;Xiao-Qing Qi ,&nbsp;Xue-Gong Li","doi":"10.1016/j.margen.2022.100982","DOIUrl":"10.1016/j.margen.2022.100982","url":null,"abstract":"<div><p><em>Gilvimarinus</em> sp. DA14, a putative new species isolated from mangrove sediment in the South China Sea (Beihai, Guangxi province), is an aerobic and heterotrophic agar degrading bacterium. Here, we present the complete genome sequence of strain DA14, which comprises 3.96 Mb sequences with 53.39% GC content. In total, 3391 CDSs, 6 rRNA genes and 44 tRNA genes were obtained. Genomic analysis of strain DA14 revealed that 218 CAZymes classes were identified and they were organized in 371 CAZymes in PULs involved in polysaccharides degradation, transport and regulation. Further, we performed the genome comparison among <em>Gilvimarinus</em> strains and analysis the diversity of CAZymes and PULs. Meanwhile, ability of agar and alginate degradation in strain DA14 were analyzed. This study represents a thorough genomic characterization of CAZymes repertoire of <em>Gilvimarinus</em>, provides insight into diversity of polysaccharide degrading enzymes existing in <em>Gilvimarinus</em> sp. DA14 and their biotechnological applications.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"65 ","pages":"Article 100982"},"PeriodicalIF":1.9,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33463307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Complete genome sequencing and analysis revealed the nitrogen utilization strategy of a novel Acuticoccus species isolated from surface water of the Indian Ocean","authors":"Jie Shi ,&nbsp;Yufeng Zeng ,&nbsp;Huan Wang ,&nbsp;Yuanyuan Niu ,&nbsp;Peiqing He ,&nbsp;Hao Chen","doi":"10.1016/j.margen.2022.100971","DOIUrl":"10.1016/j.margen.2022.100971","url":null,"abstract":"<div><p><em>Acuticoccus</em> sp. I52.16.1 was isolated from 100 m water depth from the Indian Ocean, and presented a novel <em>Acuticoccus</em> species belonging to the family Acuticoccaceae, class Alphaproteobacteria. The strain I52.16.1 displayed the activities of nitrate reductase, nitrite reductase, and urease. The genome of <em>Acuticoccus</em> sp. I52.16.1 consisted of a circular chromosome (5, 134, 086<!--> <!-->bp) with a G + C content of 69.7 mol%. The predicted number of coding genes was 4935, including 6 rRNA, 47 tRNA, and 2 sRNA. The 16S rDNA sequence displayed the maximum similarity of 97.58% with <em>Acuticoccus yangtzensis</em> JL1095^T, followed by <em>Acuticoccus sediminis</em> PTG4-2^T (97.05%), <em>Acuticoccus kandeliae</em> J103^T (96.52%), and <em>Acuticoccus mangrove</em> B2012^T (95.85%). <em>Acuticoccus</em> sp. I52.16.1 contained clades of genes involved in assimilating ammonium, nitrate, nitrite, and simple organic nitrogen compounds, but lacked the pathway for dissimilatory denitrification. Two distinct types of ureases were also detected, suggesting genetic heterogeneity. This study provided insight into the nitrogen metabolism strategies of heterotrophic bacteria in the oligotrophic ocean surface.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"65 ","pages":"Article 100971"},"PeriodicalIF":1.9,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33463306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptome responses in copepods Calanus finmarchicus, Calanus glacialis and Calanus hyperboreus exposed to phenanthrene and benzo[a]pyrene","authors":"Fekadu Yadetie ,&nbsp;Nadja R. Brun ,&nbsp;Julia Giebichenstein ,&nbsp;Katarzyna Dmoch ,&nbsp;Ketil Hylland ,&nbsp;Katrine Borgå ,&nbsp;Odd André Karlsen ,&nbsp;Anders Goksøyr","doi":"10.1016/j.margen.2022.100981","DOIUrl":"10.1016/j.margen.2022.100981","url":null,"abstract":"<div><p>Arctic and sub-arctic pelagic organisms can be exposed to effluents and spills from offshore petroleum-related activities and thus it is important to understand how they respond to crude oil related contaminants such as polycyclic aromatic hydrocarbons (PAHs). The copepod species <em>Calanus finmarchicus, Calanus glacialis</em> and <em>Calanus hyperboreus</em> represent key links in the arctic marine food web. We performed a transcriptome analysis of the three species exposed to phenanthrene (Phe) and benzo[<em>a</em>]pyrene (BaP) representing low and high molecular weight PAHs, respectively. Differential expression of several genes involved in many cellular pathways was observed after 72 h exposure to Phe (0.1 μM) and BaP (0.1 μM). In <em>C. finmarchicus</em> and <em>C. glacialis</em>, the exposure resulted in up-regulation of genes encoding enzymes in xenobiotic biotransformation, particularly the phase II cytosolic sulfonation system that include 3′-phosphoadenosine 5′-phosphosulfate synthase (PAPSS) and sulfotransferases (SULTs). The sulfonation pathway genes were more strongly induced by BaP than Phe in <em>C. finmarchicus</em> and <em>C. glacialis</em> but were not affected in <em>C. hyperboreus</em>. However, a larger number of genes and pathways were modulated in <em>C. hyperboreus</em> by the PAHs including genes encoding xenobiotic biotransformation and lipid metabolism enzymes, suggesting stronger responses in this species. The results suggest that the cytosolic sulfonation is a major phase II conjugation pathway for PAHs in <em>C. finmarchicus</em> and <em>C. glacialis</em>. Some of the biotransformation systems affected are known to be involved in metabolism of endogenous compounds such as ecdysteroids, which may suggest potential interference with physiological and developmental processes of the copepod species.</p></div>","PeriodicalId":18321,"journal":{"name":"Marine genomics","volume":"65 ","pages":"Article 100981"},"PeriodicalIF":1.9,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S1874778722000599/pdfft?md5=5318cd15fd32225027dedd984991b635&pid=1-s2.0-S1874778722000599-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40616202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}