Journal of Cytology / Indian Academy of Cytologists最新文献_第2页

Evaluation of cytomorphometric changes in tobacco users and diagnosed oral squamous cell carcinoma individuals 烟草使用者和确诊口腔鳞状细胞癌个体细胞形态变化的评价

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2016-07-01 DOI: 10.4103/0970-9371.188047

Urmila Udayashankar, V. Guduru, A. Ananthaneni, S. Ramisetty, P. H. Kuberappa, Srilekha Namala

{"title":"Evaluation of cytomorphometric changes in tobacco users and diagnosed oral squamous cell carcinoma individuals","authors":"Urmila Udayashankar, V. Guduru, A. Ananthaneni, S. Ramisetty, P. H. Kuberappa, Srilekha Namala","doi":"10.4103/0970-9371.188047","DOIUrl":"https://doi.org/10.4103/0970-9371.188047","url":null,"abstract":"Aims: To determine the cellular and nuclear area of keratinocytes in smears obtained from the oral mucosa of tobacco users, those with oral squamous cell carcinoma (OSCC), and from normal healthy persons and resolve if any significant difference exists in these three groups. Materials and Methods: The study group comprised 100 subjects 20 controls, (40 OSCC patients-20 from lesional sites and 20 from nonlesional sites, 20 tobacco smokers and 20 tobacco chewers) in the age group of 25-75 years. Oral mucosal smears obtained by using a cytobrush were stained with Papanicolaou (PAP) stain and using 20X objective in trinocular Olympus model BX53 with Jenoptik scientific grade-dedicated microphotographic camera images were taken. With ProgRes version 8.0 image analysis software, 20 cells with defined borders were evaluated from each slide. Finally, one-way analysis of variance (ANOVA) was used to compare the above parameters in the studied groups. Statistical Analysis Used: Minitab and Excel software were used to analyze the data. One-way ANOVA was used to compare the above parameters in the studied groups. Results: The mean value of the cell area for groups I, II, III, IV, and V were 2838 ± 275.2, 2762.1 ± 511.4, 2861.9 ± 512.9, 2643.8 ± 333.3, and 3064.3 ± 362.7, respectively, the nuclear area (NA) was 83.88 ± 9.86, 106.19 ± 13.45, 95.11 ± 14.24, 85.55 ± 21.11, and 80.83 ± 13.45, respectively, and nuclear-to-cellular (N:C) ratio was 0.0297, 0.03924, 0.0337, 0.03257, and 0.02678, respectively. Conclusions: Thus, our study elucidates that cytomorphology gauges the effect of tobacco on the oral mucosa and possibly establishes a link between premalignant and malignant transformations even before a lesion is visibly noted.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"371 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120877125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Cytological grading: An alternative to histological grading in oral squamous cell carcinoma 细胞学分级:口腔鳞状细胞癌的组织学分级的替代方法

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2016-07-01 DOI: 10.4103/0970-9371.188048

Srilekha Namala, V. Guduru, A. Ananthaneni, S. Devi, P. H. Kuberappa, Urmila Udayashankar

{"title":"Cytological grading: An alternative to histological grading in oral squamous cell carcinoma","authors":"Srilekha Namala, V. Guduru, A. Ananthaneni, S. Devi, P. H. Kuberappa, Urmila Udayashankar","doi":"10.4103/0970-9371.188048","DOIUrl":"https://doi.org/10.4103/0970-9371.188048","url":null,"abstract":"Background: Micronuclei (MN) in oral exfoliative cells have been shown to indicate a disparaging change in genetic information of the cell. Recent studies showed correlation between the frequency of MN and severity of this damage. Grading of lesions can be used to determine the austerity of this damage. Aims: The aim of this study is to compare the MN frequency in oral exfoliated cells of normal and oral squamous cell carcinoma (OSCC) individuals and to cytologically grade the frequency of MN in cytological smears and to correlate it with histological grading. The objective is to ascertain whether MN frequency in oral exfoliated cells can be a parameter for grading of OSCC. Settings and Design: The study group comprises of 40 subjects (20 controls and 20 OSCC patients) in the age group of 45-85 years. Materials and Methods: The cytosmear was obtained from each group and stained with Papanicolaou (PAP) stain. Twenty cells from each slide were counted for MN and cytological grade of OSCC was assigned based on the average frequency of MN. Cytological grade was correlated with histological grading and the data were recorded. Student′s t-test and Spearman′s correlation were used for the analysis of the data. Results: Average frequency of MN was 2.5 times higher in OSCC patients when compared to that in controls and the difference was found to be highly significant. Sixty percent correlation was found between cytological grade and histological grade of OSCC and the difference between them was not significant. Conclusions: Cytological grading can be used in grading OSCC, and MN insinuates genotoxic damage occurring in the epithelial cells.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130071594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Destaining of Diff-Quik stained cytologic smears is not necessary for the detection of anaplastic lymphoma kinase gene rearrangement in lung adenocarcinoma by fluorescence in situ hybridization 荧光原位杂交检测肺腺癌间变性淋巴瘤激酶基因重排不需要染色细胞学涂片

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2016-07-01 DOI: 10.4103/0970-9371.188061

Weisheng Xu, K. Khurana, Jamie Tull, Charlene Maciak, Shengle Zhang

{"title":"Destaining of Diff-Quik stained cytologic smears is not necessary for the detection of anaplastic lymphoma kinase gene rearrangement in lung adenocarcinoma by fluorescence in situ hybridization","authors":"Weisheng Xu, K. Khurana, Jamie Tull, Charlene Maciak, Shengle Zhang","doi":"10.4103/0970-9371.188061","DOIUrl":"https://doi.org/10.4103/0970-9371.188061","url":null,"abstract":"Background: Anaplastic lymphoma kinase (ALK) gene rearrangement analysis by fluorescence in situ hybridization (FISH) is one of the standard molecular tests for targeted therapy of lung adenocarcinoma. However, insufficient cell block cellularity may impede molecular testing. A recent study showed that Diff-Quik (DQ) stained cytology smear is suitable for ALK by FISH. Aims: The aim of our study was to observe the impact of destaining intervals on the quality of FISH signals and determine if DQ smears without destaining would allow FISH analysis. Materials and Methods: Thirty-five DQ smears from 27 cases of lung adenocarcinoma were analyzed for ALK gene rearrangement by FISH. Twenty three DQ smears were destained for different intervals, including 30 s (13 cases), 1 min (6 cases), or 2 min (4 cases). Twelve DQ smears were not subjected to destaining. For further validation, FISH signals in 8 smears and 6 cell blocks were compared with the paired destained DQ smears. The signal quality was semi-quantified and analyzed with Chi-squared test. Results: Of the total 27 selected cases, three (11%) were positive for ALK gene rearrangement, whereas 24 (89%) were negative. FISH signal was satisfactory in all DQ smears. There was no significant difference in the quality of signal among smears with different destaining intervals (P = 0.55) or between smears with and without destaining (P = 0.41). DQ smears without destaining showed identical FISH results and similar or better signals as compared with paired destained smears and cell blocks in all cases. Conclusions: Duration of destaining intervals does not impact the quality of FISH signal on DQ smears. Destaining of DQ smears is not necessary for ALK by FISH.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"127 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132911517","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Bacterial vaginosis in association with spontaneous abortion and recurrent pregnancy losses 细菌性阴道病与自然流产和反复流产有关

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2016-07-01 DOI: 10.4103/0970-9371.188050

Gözde Işık, Ş. Demirezen, H. Dönmez, M. Beksaç

{"title":"Bacterial vaginosis in association with spontaneous abortion and recurrent pregnancy losses","authors":"Gözde Işık, Ş. Demirezen, H. Dönmez, M. Beksaç","doi":"10.4103/0970-9371.188050","DOIUrl":"https://doi.org/10.4103/0970-9371.188050","url":null,"abstract":"Context: Bacterial vaginosis (BV) is related to the increased risk of miscarriage, preterm labor, and postpartum endometritis. Aims: The aim of this study was to evaluate the association between BV and the history of spontaneous abortion and recurrent pregnancy losses. We also examined periods of gestation, including the first and second trimester miscarriages. Materials and Methods: The study population consisted of 200 fertile women. Sixty one (30.5%) of 200 women had the history of a spontaneous abortion in the last six months (N = 30) and at least three recurrent pregnancy losses (N = 31). BV was diagnosed either by using Papanicolaou staining, Gram staining, or by culturing with BV-associated bacteria, Gardnerella vaginalis. Results: The presence of BV was statistically associated with the history of a spontaneous abortion in the last 6 months (P < 0.05), whereas there was no significant relationship between BV and recurrent pregnancy losses (P > 0.05). These women were also evaluated in view of periods of gestation. Forty-seven (77%) of 61 women had first trimester miscarriage (≤12 weeks) and 14 (23%) of 61 women had second trimester miscarriage (>12 weeks). There was a statistically significant relationship between BV and second trimester miscarriage (P < 0.05). Positive BV findings were not associated with discharge, itching, and pain (P > 0.05). Conclusion: BV may contribute to spontaneous abortion and second trimester miscarriage.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"72 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121955707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 71

Fine needle aspiration cytology of vulvar actinomycosis masquerading clinically as malignancy 临床伪装为恶性外阴放线菌病的细针吸细胞学分析

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171250

Rahul Parasram Wanjari, Suwarna B. Patil, M. Khade, Dilip S. Sarate

引用次数: 1

Custom made filter card for cytospin: A high fidelity economical alternative 定制的细胞自旋滤卡:一个高保真经济的选择

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171258

V. Krishnamurthy, S. M. Doreswamy

引用次数: 1

Optimization of FNAC findings as a preoperative diagnostic aid for odontogenic cysts 优化FNAC表现作为牙源性囊肿术前诊断的辅助手段

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171238

G. Jain, P. Shetty

引用次数: 2

Conjunctival impression cytology versus routine tear function tests for dry eye evaluation in contact lens wearers 结膜印象细胞学与常规泪液功能测试对隐形眼镜佩戴者干眼评估的影响

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171242

Prachi Kumar, R. Bhargava, Y. Arora, Sidharth Kaushal, Manjushri Kumar

{"title":"Conjunctival impression cytology versus routine tear function tests for dry eye evaluation in contact lens wearers","authors":"Prachi Kumar, R. Bhargava, Y. Arora, Sidharth Kaushal, Manjushri Kumar","doi":"10.4103/0970-9371.171242","DOIUrl":"https://doi.org/10.4103/0970-9371.171242","url":null,"abstract":"Aims: Prolonged contact lens wear is often accompanied by dryness of the eyes. The aim of this study was to compare conjunctival impression cytology (CIC) and tear film tests such as tear film break up time (TBUT) and Schirmer test for dry eye evaluation in contact lens wearers and measure their correlation with dry eye symptoms. Setting: A case control study was done at three referral eye centers. Materials and Methods: The eyes of 230 contact lens users were compared to 250 eyes of age- and sex-matched controls. Participants were recruited based on their response to a questionnaire of dry eye symptoms, (Dry Eye Scoring System, DESS©) and measurements of TBUT, Schirmer test, and CIC was done. A correlation analysis between symptom severity and tear film tests was performed. Pearson's coefficient, R2 > 0.5 was considered significant. Results: As compared to controls (r2 = 0.010), Nelson grade correlated significantly with dry eye symptoms (r2 = 0.765), among cases. However, there was moderate correlation between dry eye symptoms, Schirmer test, and TBUT (r2 = 0.557 and 0.530, respectively) among cases and a weak correlation among controls (r2 = 0.130 and 0.054, respectively). The sensitivity of TBUT was 86.4%, specificity was 82.4%, positive likelihood ratio (LR) was 4.50 [95% confidence interval (CI) 3.46-5.85)], and negative LR was 0.09. The sensitivity of the Schirmer test was 48.2%, specificity 88%, LR 2.12 (95% CI 1.48-2.96), and negative LR 0.83. Conclusion: CIC correlates better than Schirmer and TBUT with dry eye symptoms. It may be the most appropriate test for dry-eye evaluation in contact lens wearers.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"66 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122627797","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Lymphangioleiomyomatosis diagnosed by effusion cytology: A case report 积液细胞学诊断淋巴管平滑肌瘤病1例

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171256

G. Rivera, T. Gokaslan, Elizabeth M. Kurian

引用次数: 1

Bilaterally enlarged parotids and sicca symptoms as a presentation of sarcoidosis: Pivotal role of aspiration cytology in diagnosis 双侧腮腺肿大和干燥症状作为结节病的表现:吸痰细胞学在诊断中的关键作用

Journal of Cytology / Indian Academy of Cytologists Pub Date : 2015-10-01 DOI: 10.4103/0970-9371.171254

T. Sharma, D. Joshi, A. Khurana, Vikas Gupta, N. Kapoor

{"title":"Bilaterally enlarged parotids and sicca symptoms as a presentation of sarcoidosis: Pivotal role of aspiration cytology in diagnosis","authors":"T. Sharma, D. Joshi, A. Khurana, Vikas Gupta, N. Kapoor","doi":"10.4103/0970-9371.171254","DOIUrl":"https://doi.org/10.4103/0970-9371.171254","url":null,"abstract":"Sarcoidosis is a chronic multisystem inflammatory disease of unknown etiology characterized by widespread noncaseating granulomas in various organs. The diagnosis of sarcoidosis is based on cytological, clinicolaboratory, and radiological findings, and requires careful exclusion of other granulomatous diseases especially tuberculosis. Involvement of parotid glands is uncommon. Presentation of sarcoidosis with sicca symptoms that include dryness of eyes and mouth is an even rarer phenomenon. We present a case of multisystemic sarcoidosis presenting with dryness of eyes and mouth along with bilateral enlargement of parotid glands. Fine-needle aspiration cytology (FNAC) smears showed epithelioid cell granulomas and multinucleate giant cells. Stain for acid-fast bacilli was negative. Careful cytological examination revealed crystalline structures inside the giant cells, which prompted us to evaluate the patient for sarcoidosis. This case report highlights the cytological features that can be useful in clinching the diagnosis of sarcoidosis in conjunction with clinicoradiological and laboratory findings in a clinically unusual case.","PeriodicalId":182593,"journal":{"name":"Journal of Cytology / Indian Academy of Cytologists","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122359663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7