Immunology & Cell Biology最新文献

{"title":"Choose your own T-cell fate: creation of a narrative-based, decision-making activity to engage students in immunology","authors":"Helen E Ritchie,&nbsp;Gareth Denyer,&nbsp;Kylie E Webster","doi":"10.1111/imcb.12833","DOIUrl":"10.1111/imcb.12833","url":null,"abstract":"<p>Undergraduate courses in immunology are content-heavy and combined with a new, complex vocabulary, can be an overwhelming subject for students. In-class active learning approaches have been found to improve understanding of difficult concepts in science, technology, engineering and mathematics (STEM) disciplines; however, many undergraduate courses maintain a high dependence on lecture-style teaching because of time constraints, content demands and student resistance. We designed an online, out-of-class activity, the “Life and Death of a T cell”, to complement a lecture on a complex immunological concept, T-cell development. Inspired by the “Choose Your Own Adventure” children's books, a fictional narrative was created in which students assume the role of a cell with a dream of becoming a helper T cell. Decision-making scenarios then prompt students to draw on their knowledge from the lecture to successfully navigate the steps of T-cell development. The activity was built on two platforms, Google Forms and H5P (HTML 5 Package), both of which are readily accessible and allow the inclusion of branching logic and the creation of a decision tree–based activity. An anonymous survey revealed that students found this interactive approach enjoyable, and their perceived understanding of the content significantly increased. Students appreciated the inclusion of a novel learning resource, with requests for similar activities to be developed for other immunological concepts. In conclusion, we developed a narrative-based, decision-making activity to complement a lecture on T-cell development. As an out-of-class activity, this style of learning approach can potentially capitalize on the benefits of active learning, while also overcoming barriers of student resistance.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"103 2","pages":"149-160"},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of clonally expanded γδ T-cell populations during CAR-T cell therapy","authors":"Arman Safavi,&nbsp;Jerome Samir,&nbsp;Mandeep Singh,&nbsp;Martina Bonomi,&nbsp;Raymond Yip Louie,&nbsp;Kenneth Micklethwaite,&nbsp;Fabio Luciani","doi":"10.1111/imcb.12834","DOIUrl":"10.1111/imcb.12834","url":null,"abstract":"<p>Anti-CD19 Chimeric Antigen Receptor (CAR)-T cell therapies have shown promise for treating B cell malignancies, but the clinical outcome is influenced by both the CAR-T product and the patient's immune system. The role of γδ T cells in the context of CAR-T cell therapy remains poorly understood. This study investigates the transcriptional heterogeneity, clonal expansion and dynamics of γδ T cells in patients undergoing anti-CD19 CAR-T cell therapy. Longitudinal single cell multi-omics analysis was performed on γδ T cells from four patients receiving anti-CD19 CAR-T cell therapy. Single cell RNA-seq, antibody-based protein profiling (AbSeq) and full-length TCRγδ sequences revealed clonally expanded populations displaying plasticity in T cell differentiation, and temporal dynamics of large clones, suggesting ongoing expansion and differentiation. Clonally expanded γδ T cells had heterogeneous gene expression profiles, occupying seven transcriptionally distinct clusters. Analysis of chemokine markers indicated cluster-specific homing tendencies of circulating γδ T cells to peripheral tissues. We found unexpectedly high frequencies of Vδ1 and Vδ3 cells in the blood with distinct gene and protein expression profiles. This analysis provides insights into the dynamic and heterogeneous nature of γδ T cells following anti-CD19 CAR-T cell therapy, contributing valuable information for optimizing CAR-T cell therapies in B cell malignancies.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"103 1","pages":"60-72"},"PeriodicalIF":3.2,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142581421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The power of networking in science and academia","authors":"Jessica G Borger","doi":"10.1111/imcb.12832","DOIUrl":"10.1111/imcb.12832","url":null,"abstract":"<p>In science and academia, success is often shaped by both knowledge and networking. Reflecting on nearly two decades in academic research, I recount my experience as a postdoctoral immunologist returning to Australia with limited local connections and support. Upon re-establishing myself in Australia, I initially faced barriers that restricted my visibility and collaborations. A turning point came when personal challenges motivated me to actively network, leading to valuable collaborations and career opportunities. By initiating conversations with academic leaders and peers, I expanded my network and established numerous leadership roles, even as a “junior” postdoc through founding a symposium, engaging with an immunology society, volunteering on various academic and advocacy committees, contributing to public outreach and nationally advocating for gender equity in science. These experiences reinforced that networking is about fostering meaningful relationships and creating opportunities to grow professionally. I provide advice on how to increase your networks by volunteering at work, when attending conferences, through contributing to societies and building a social media presence. My journey highlights the importance of being proactive in building networks, which can open doors, amplify one's voice, and drive career advancement in science and academia.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 10","pages":"871-877"},"PeriodicalIF":3.2,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12832","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Altered immunophenotypic expression in the peripheral bladder cancer immune landscape","authors":"Nathan J Mackenzie,&nbsp;Kate Zimmermann,&nbsp;Clarissa Nicholls,&nbsp;Mahasha PJ Perera,&nbsp;Alexander Ngoo,&nbsp;Penny L Jeffery,&nbsp;Ian Vela,&nbsp;Tony J Kenna,&nbsp;Elizabeth D Williams,&nbsp;Patrick B Thomas","doi":"10.1111/imcb.12829","DOIUrl":"10.1111/imcb.12829","url":null,"abstract":"<p>Treatments targeting the immune system only benefit a subset of patients with bladder cancer (BC). Biomarkers predictive of BC progression and response to specific therapeutic interventions are required. We evaluated whether peripheral blood immune subsets and expression of clinically relevant immune checkpoint markers are associated with clinicopathologic features of BC. Peripheral blood mononuclear cells isolated from blood collected from 23 patients with BC and 9 age-matched unaffected-by-cancer control donors were assessed using a 21-parameter flow cytometry panel composed of markers of T, B, natural killer and myeloid populations and immune checkpoint markers. Patients with BC had significantly lower numbers of circulating CD19⁺ B cells and elevated circulating CD4⁺CD8⁺ T cells compared with the control cohort. Immune checkpoint markers programmed cell death protein 1 (PD-1) and T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) were elevated in the total peripheral immune cell population in patients with BC. Within the BC cohort, PD-1 expression in T and myeloid cells was elevated in muscle-invasive compared with non–muscle-invasive disease. In addition, elevated T, B and myeloid PD-1 cell surface expression was significantly associated with tumor stage, suggesting that measures of peripheral immune cell exhaustion may be a predictor of tumor progression in BC. Finally, positive correlations between expression levels of the various immune checkpoints both overall and within key peripheral blood immune subsets collected from patients with BC were observed, highlighting likely coregulation of peripheral immune checkpoint expression. The peripheral blood immunophenotype in patients with BC is altered compared with cancer-free individuals. Understanding this dysregulated immune profile will contribute to the identification of diagnostic and prognostic indicators to guide effective immune-targeted, personalized treatments.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 10","pages":"949-962"},"PeriodicalIF":3.2,"publicationDate":"2024-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542356","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The multiple roles of macrophages in peritoneal adhesion","authors":"Shangwei Yang,&nbsp;Yanhe Zheng,&nbsp;Zhenjun Pu,&nbsp;Hongyu Nian,&nbsp;Junliang Li","doi":"10.1111/imcb.12831","DOIUrl":"10.1111/imcb.12831","url":null,"abstract":"<p>Peritoneal adhesion (PA) refers to the abnormal adhesion of the peritoneum either with the peritoneum itself or with tissues and organs that is caused by abdominopelvic surgery, abdominal infection or peritoneal inflammation. PA is associated with various clinical complications, such as abdominal pain and distension, intestinal obstruction, gastrointestinal disorders and female infertility, and adversely affects the quality of life of patients. Macrophages are essential for PA formation and can undergo polarization into classically activated macrophages (M1) and alternatively activated macrophages (M2), which are influenced by the peritoneal microenvironment. By releasing proinflammatory cytokines and reactive oxygen species, M1 macrophages promote peritoneal inflammatory reactions and the resultant formation of adhesion. In contrast, M2 macrophages secrete anti-inflammatory cytokines and growth factors to inhibit PA formation and to promote repair and healing of peritoneal tissues, and thereby play a significant anti-inflammatory role. This review comprehensively explores the function and mechanism of macrophages and their subtypes in PA formation to gain insight into the prevention and treatment of PA based on the modulation of macrophages.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"103 1","pages":"31-44"},"PeriodicalIF":3.2,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12831","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142542357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strive, Thrive & Survive: embracing challenges in pursuit of passion","authors":"Abolaji Samson Olagunju","doi":"10.1111/imcb.12827","DOIUrl":"10.1111/imcb.12827","url":null,"abstract":"<p>Challenges don’t last; embracing them is crucial to growth and success. Knowing and absorbing this is very important for students in any program and at any level in the academic world. I have my bachelor’s and master’s degrees from Ladoke Akintola University of Technology and University of Ibadan, Nigeria, respectively. Currently, I am a doctorate student at the Department of Immunology, University of Sao Paulo, Brazil. This article discusses my adaptation to a new environment, overcoming challenges, and the importance of support systems.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"103 1","pages":"19-21"},"PeriodicalIF":3.2,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12827","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunology & Cell Biology Publication of the Year Awards 2023","authors":"Adrian Liston","doi":"10.1111/imcb.12830","DOIUrl":"10.1111/imcb.12830","url":null,"abstract":"<p>The <i>Immunology &amp; Cell Biology</i> Publication of the Year Awards have been established for outstanding studies submitted by first authors who are financial members of the Australian &amp; New Zealand Society for Immunology Inc. in the year of the article's publication. Articles vying for these awards can come from any of the journal categories including Original Articles, Outstanding Observations, Perspectives or Short Communications. The Journal undertakes rigorous review to identify the most outstanding original research articles based on scientific excellence. The winner of the Chris and Bhama Parish ICB Publication of the Year Award is awarded an AU$1000 scholarship provided by Wiley and the runner-up is awarded an AU$500 scholarship provided by Miltenyi.</p><p>Every year, an outstanding series of papers are submitted for consideration for the prizes and 2023 was no different, with an exceptional standard of science reported in the papers. It is a great pleasure to announce the winners of the awards for 2023 as follows:</p><p>The award-winning papers by Drs Kedzierski and von Borstel highlight the outstanding quality of the work published in <i>Immunology &amp; Cell Biology</i>. My very best congratulations are extended to the awardees on their success. I also thank our sponsor Miltenyi for their support of outstanding science and scientists and the journal. It is hoped that the outstanding quality of these awarded publications will also encourage others to consider <i>Immunology &amp; Cell Biology</i> as a key journal for their cutting-edge research.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 10","pages":"866-867"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12830","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of cellular and molecular immune components of the painted white sea urchin Lytechinus pictus in response to bacterial infection","authors":"Katherine T Nesbit,&nbsp;Alexis Cody Hargadon,&nbsp;Gloria D Renaudin,&nbsp;Nicholas D Kraieski,&nbsp;Katherine M Buckley,&nbsp;Emily Darin,&nbsp;Yoon Lee,&nbsp;Amro Hamdoun,&nbsp;Catherine S Schrankel","doi":"10.1111/imcb.12828","DOIUrl":"10.1111/imcb.12828","url":null,"abstract":"<p>Sea urchins are basal deuterostomes that share key molecular components of innate immunity with vertebrates. They are a powerful model for the study of innate immune system evolution and function, especially during early development. Here we characterize the morphology and associated molecular markers of larval immune cell types in a newly developed model sea urchin, <i>Lytechinus pictus</i>. We then challenge larvae through infection with an established pathogenic <i>Vibrio</i> and characterize phenotypic and molecular responses. We contrast these to the previously described immune responses of the purple sea urchin <i>Strongylocentrotus purpuratus</i>. The results revealed shared cellular morphologies and homologs of known pigment cell immunocyte markers (<i>PKS, srcr142</i>) but a striking absence of subsets of perforin-like <i>macpf</i> genes in blastocoelar cell immunocytes. We also identified novel patterning of cells expressing a scavenger receptor cysteine rich (SRCR) gene in the coelomic pouches of the larva (the embryonic stem cell niche). The SRCR signal becomes further enriched in both pouches in response to bacterial infection. Collectively, these results provide a foundation for the study of immune responses in <i>L. pictus</i>. The characterization of the larval immune system of this rapidly developing and genetically enabled sea urchin species will facilitate more sophisticated studies of innate immunity and the crosstalk between the immune system and development.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"103 1","pages":"45-59"},"PeriodicalIF":3.2,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11688612/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142491655","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"ADAM10 modulates the efficacy of T-cell-mediated therapy in solid tumors","authors":"Ahmed ME Abdalla,&nbsp;Yu Miao,&nbsp;Ning Ming,&nbsp;Chenxi Ouyang","doi":"10.1111/imcb.12826","DOIUrl":"10.1111/imcb.12826","url":null,"abstract":"<p>T-cell-mediated therapeutic strategies are the most potent effectors of cancer immunotherapy. However, an essential barrier to this therapy in solid tumors is disrupting the anti-cancer immune response, cancer-immunity cycle, T-cell priming, trafficking and T-cell cytotoxic capacity. Thus, reinforcing the anti-cancer immune response is needed to improve the effectiveness of T-cell-mediated therapy. Tumor-associated protease ADAM10, endothelial cells (ECs) and cytotoxic CD8⁺ T cells engage in complex communication <i>via</i> adhesion, transmigration and chemotactic mechanisms to facilitate an anti-cancer immune response. The precise impact of ADAM10 on the intricate mechanisms underlying these interactions remains unclear. This paper broadly explores how ADAM10, through different routes, influences the efficacy of T-cell-mediated therapy. ADAM10 cleaves CD8⁺ T-cell-targeting genes and impacts their expression and specificity. In addition, ADAM10 mediates the interactions of adhesion molecules with T cells and influences CD8⁺ T-cell activity and trafficking. Thus, understanding the role of ADAM10 in these events may lead to innovative strategies for advancing T-cell-mediated therapies.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 10","pages":"907-923"},"PeriodicalIF":3.2,"publicationDate":"2024-10-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12826","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune repertoire profiling in myasthenia gravis","authors":"Ting He,&nbsp;Kangzhi Chen,&nbsp;Qian Zhou,&nbsp;Haobing Cai,&nbsp;Huan Yang","doi":"10.1111/imcb.12825","DOIUrl":"10.1111/imcb.12825","url":null,"abstract":"<p>Myasthenia gravis (MG) is the most frequent immune-mediated neurological disorder, characterized by fluctuating muscle weakness. Specific recognition of self-antigens by T-cell receptors (TCRs) and B-cell receptors (BCRs), coupled with T–B cell interactions, activates B cells to produce autoantibodies, which are critical for the initiation and perpetuation of MG. The immune repertoire comprises all functionally diverse T and B cells at a specific time point in an individual, reflecting the essence of immune selectivity. By sequencing the nucleotide sequences of TCRs and BCRs, it is possible to track individual T- and B-cell clones. This review delves into the generation of autoreactive TCRs and BCRs in MG and comprehensively examines the applications of immune repertoire sequencing in understanding disease pathogenesis, developing diagnostic and prognostic markers and informing targeted therapies. We also discuss the current limitations and future potential of this approach.</p>","PeriodicalId":179,"journal":{"name":"Immunology & Cell Biology","volume":"102 10","pages":"891-906"},"PeriodicalIF":3.2,"publicationDate":"2024-10-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/imcb.12825","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142454136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}