Laboratory Animal Research最新文献

{"title":"Application of exosomes derived from human umbilical cord stem cells in the treatment of ovalbumin-induced allergic asthma in mice.","authors":"Yong Ding, Yanli Zhang, Qi Cao, Yong Yin, Zhenglai Ma, Wenxia Pan, Xinjie Du, Chunling Ma","doi":"10.1186/s42826-026-00280-y","DOIUrl":"10.1186/s42826-026-00280-y","url":null,"abstract":"<p><strong>Background: </strong>This study investigated the therapeutic potential of exosomes derived from human umbilical cord mesenchymal stem cells (hUCMSCs-exos) in an Ovalbumin (OVA) -induced mouse model of allergic asthma, comparing their efficacy to budesonide, a standard asthma treatment. hUCMSCs-exos were characterized by their typical morphology, size, and marker expression (CD9, CD63, and HSP70) using transmission electron microscopy (TEM), Nanosight 300, and Western blot analysis.</p><p><strong>Results: </strong>Treatment with hUCMSCs-exos significantly decreased lung inflammation by detecting plasma IL-4 and IgE levels, as well as white blood cells and eosinophils counts in Bronchoalveolar lavage fluid (BALF), the M1 and M2 type of macrophages, Regulatory T Cells (Tregs) in lung tissues, paralleling the effects of budesonide therapy. Notably, hUCMSCs -exos achieved a greater reduction in the percentage of eosinophils in BALF and a significant increase M2 macrophages and Tregs in lung tissues.</p><p><strong>Conclusions: </strong>These findings suggest hUCMSCs-exos may offer comparable or superior therapeutic benefits to budesonide, warranting further investigation into their molecular mechanisms to support clinical application.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13182008/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sepsis as a confounding factor in assessing liver dysfunction in parenterally fed piglets: a model for human infants with intestinal failure.","authors":"Mahabub Alam, Pamela R Wizzard, Patrick N Nation, Michael Zaugg, Paul W Wales, Justine M Turner","doi":"10.1186/s42826-026-00279-5","DOIUrl":"10.1186/s42826-026-00279-5","url":null,"abstract":"<p><strong>Background: </strong>Sepsis is a common complication of parenteral nutrition therapy for infants with intestinal failure, due to the presence of a central venous catheter and impairment of gut barrier function. Intestinal failure-associated liver disease (IFALD) is another complication, often attributed to the type of intravenous lipid emulsion delivered and this is commonly studied in pre-clinical animal models. Animal studies frequently overlook sepsis as a confounding factor, potentially biasing the results. We retrospectively reviewed 14-day studies of total parenteral nutrition (TPN) in piglets in our laboratory from 2011 to 2025, to determine if sepsis is an independent predictor of key liver outcomes relevant to IFALD research. Sepsis was defined as positive blood culture.</p><p><strong>Results: </strong>A total of 86 piglets (76 male; aged 2-5 days) were eligible for inclusion across four experimental studies. In total, 44 (51%) were suspected to have sepsis on the basis of clinical signs, 4 had missing blood cultures, and of these 28/40 (70%) had a positive blood culture. Septic piglets had higher mortality compared to those not suspected to be septic or having a negative blood culture (43% vs. 9%, p < 0.001). Septic piglets showed significantly lower bile flow (p < 0.001) and higher serum total bilirubin (p < 0.001) compared to non-septic piglets. The risk of developing sepsis was reduced in piglets with older age (OR = 0.42, p = 0.007) and increased when given a pure soy-oil compared to pure fish-oil emulsion (OR = 10.77, p = 0.05). Multivariate analysis showed the independent predictors of bile flow were both sepsis (negative) and higher body weight at study entry (positive) (R² = 0.42, p < 0.001), while sepsis and use of a soy-based lipid emulsion were independent positive predictors of total bilirubin (R² = 0.34, p < 0.001).</p><p><strong>Conclusions: </strong>Sepsis is associated with reduced bile flow, elevated total bilirubin and increased mortality in PN fed neonatal piglets. As sepsis can be a confounding factor in liver outcomes, researchers should both introduce refinements to mitigate sepsis as well as consistently report on the number of animals with sepsis within treatment groups and their outcomes.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13147880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147839682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cross-talk of gut-bone-muscle: osteosarcopenia in experimental colitis models.","authors":"Shilpa Sharma","doi":"10.1186/s42826-026-00273-x","DOIUrl":"10.1186/s42826-026-00273-x","url":null,"abstract":"","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13077943/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intracerebroventricular streptozotocin-induced animal model of Alzheimer's disease: revealing dose optimization, administration regimen, and molecular pathways.","authors":"Sagar A More, Radhika N Mundke, Yogeeta O Agrawal, Sanjay N Awathale, Sameer N Goyal, Kartik T Nakhate, Sumit S Rathod","doi":"10.1186/s42826-026-00278-6","DOIUrl":"10.1186/s42826-026-00278-6","url":null,"abstract":"<p><p>Streptozotocin (STZ) is a commonly administered chemical via the intracerebroventricular (ICV) route in rodents to induce Alzheimer’s disease (AD)-like symptoms. Unfortunately, available research articles from different laboratories suggest inconsistency in doses, administration schedules, and target brain regions. For instance, ICV dose varies from 1.5 to 5 mg/kg either unilaterally or bilaterally, for 7 days to 21 days, as a single or multiple injections. Therefore, it is challenging for novice investigators to select the optimal doses, treatment durations, and targeted molecular pathways while employing STZ to induce AD-like conditions in experimental animals. In this background, the information related to the STZ-induced animal model of AD should be available on a single platform to aid researchers in selecting the suitable doses and regimens that suit their research objectives. The literature search was carried out by employing search engines such as PubMed and Google Scholar with keywords such as streptozotocin, intracerebroventricular, dosage optimization, Alzheimer’s disease, neuroinflammation, and oxidative stress. The present review highlights the articles published up to May 2025, with predefined inclusion (ICV-STZ model, cognitive/biochemical outcomes) and exclusion criteria (non-peer-reviewed papers, studies lacking behavioral or molecular endpoints, and studies older than 20 years). We have attempted to present the optimal dose, administration regimen, and biological process for inducing ICV-STZ AD models based on their advantages and limitations. According to the comparative quantitative evaluation of preclinical investigations, bilateral ICV administration of STZ at a cumulative dose of 3 mg/kg (1.5 mg/kg on days 1 and 3) displays the most reliable and physiologically relevant regimen. Studies also demonstrated that between 14 and 21 days after injection, this regimen reliably causes oxidative stress, neuroinflammation, cholinergic dysfunction, and progressive cognitive impairments, offering a balanced picture of physiological, histological, and behavioral alterations. However, unlike transgenic models, the ICV-STZ paradigm generally fails to develop extensive amyloid-beta plaque deposition or well-formed neurofibrillary tangles. Hence, more quantitative investigations are necessary to validate this optimization.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term effect of ovary removal on the joints of aged mice.","authors":"Sofia Wüstenhagen, Lindsay Zentveld, Francesco Longo, Loise Råberg, Alexandra Stubelius, Riddhi Vyas, Anders Nguyen, Julia M Scheffler, Mattias N D Svensson, Carmen Corciulo","doi":"10.1186/s42826-026-00271-z","DOIUrl":"10.1186/s42826-026-00271-z","url":null,"abstract":"<p><p>BACKGROUND: Age-related diseases are medical conditions whose incidence or severity increases with advancing age. Among them, musculoskeletal diseases, like osteoarthritis, are associated with postmenopausal hormonal shifts. Ovariectomy (OVX) is a widely used animal model to mimic the decline of ovarian hormones after menopause. Most preclinical studies rely on ovariectomized young animals, questioning their relevance to faithfully replicate the complexities of the human conditions. This study examines the long-term effects of OVX on motor performance, nociceptive response, and joint morphology in adult mice, all of which are osteoarthritis-related characteristics. Seven-month-old female mice underwent OVX or sham surgery and were monitored longitudinally until 18 months of age. Motor ability was assessed using rotarod and open field tests, pain sensitivity was evaluated via Von Frey testing, and bone integrity was analyzed through micro-computed tomography (µCT). Histological evaluation of articular cartilage was performed using Safranin-O staining. RESULTS: A transient increase in mechanical pain sensitivity was observed between 10 and 13 months post-OVX. Despite this change, OVX did not exacerbate the age-related decline in motor function. µCT revealed reduced bone mineral density in the subchondral cortical and vertebral trabecular bone in OVX mice, without significant changes in trabecular volume. Articular cartilage degeneration was similar in both experimental groups. CONCLUSIONS: These findings suggest that hormonal depletion alone may not be sufficient to drive the full osteoarthritic phenotype in aging mice. Importantly, longitudinal studies allow the capture of subtle differences in the aging process. </p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13072458/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147674751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Susceptibility and transcriptional characteristics of inbred C57BL/6J and outbred ICR mice in CDAHFD-induced MASH models.","authors":"Jing Zhou, Bingyi Li, Ziyi Guo, Bing Zhou, Yan Lu, Lian Zhu, Jin Lu, Jian Wang, E Wang, Yao Li","doi":"10.1186/s42826-026-00272-y","DOIUrl":"10.1186/s42826-026-00272-y","url":null,"abstract":"<p><p>BACKGROUND: Metabolic dysfunction-associated steatohepatitis (MASH) is a progressive fibrotic liver disease, the underlying mechanisms of which have not been fully elucidated. Most MASH research relies on diet-induced models, particularly inbred mouse strains such as C57BL/6J. Although inbred strains are commonly used, outbred mice more accurately reflect the genetic diversity of human populations. It was reported that ICR mice were resistant to developing MASH after high-fat diet feeding. However, the effects of choline-deficient, L-amino acid-defined, high-fat diet (CDAHFD) on ICR mice remain unexplored. The CDAHFD is a widely adopted diet-induced model for MASH. In this study, C57BL/6J and ICR mice were fed either a normal diet (ND) or a CDAHFD for 10 weeks. Metabolism-related phenotypes and liver histology assessments were conducted to establish MASH models. RNA transcriptome sequencing of liver samples was performed to identify differentially expressed genes, which were then aligned to the human MASH transcriptome. RESULTS: We successfully established MASH models via CDAHFD in both C57BL/6J and ICR mouse strains. ICR mice presented transcriptional profiles comparable to those of the C57BL/6J strain and effectively replicated the inflammatory and fibrotic features observed in patients with MASH. CONCLUSIONS: This study revealed that ICR mice are as suitable as inbred C57BL/6J mice for CDAHFD-induced MASH models. </p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059155/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639352","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel blood collection technique from the rat thoracic aorta: employing a diaphragm sac as a reservoir.","authors":"Purvaj G Barote, Abdulla Sherikar, Sameer N Goyal, Sanjay N Awathale","doi":"10.1186/s42826-026-00277-7","DOIUrl":"10.1186/s42826-026-00277-7","url":null,"abstract":"<p><p>BACKGROUND: Several blood withdrawal techniques are employed for biochemical research. However, these techniques fail to fulfil the desired requirement of blood volume. We invented the alternative method of blood collection through the thoracic aorta in an anesthetized rat. Under anesthesia, the rat was placed supine, and a transverse abdominal incision was made. The diaphragm was carefully cut, and a vertical thoracic incision was performed. The rat was held upright, creating a diaphragm sac, as a natural reservoir for blood collection. The thoracic aorta was identified and cut, allowing a large volume of blood to accumulate and be collected in sample tubes. The purity of the blood samples was determined using the plasma protein concentration, hemolysis index and lactate dehydrogenase (LDH) activity. RESULTS: A maximum blood volume of approximately 10–15 mL was successfully collected from the thoracic aorta using the diaphragm sac in a single rat. This method provided a higher blood yield compared to traditional techniques, such as retro-orbital bleeding, tail vein sampling, and cardiac puncture, all of which typically yield smaller volumes and require multiple collections. The blood collected using this method demonstrated comparable quality to that obtained via cardiac puncture, with no significant differences observed in plasma protein concentration, hemolysis index and LDH activity, supporting its suitability for high-quality plasma collection. CONCLUSIONS: Existing blood collection methods often fall short of volume requirements, leading to repeated needle and capillary use and causing animal stress. In contrast, the proposed method is simpler, designed for rats sacrificed post-procedure. The new blood collection method through the thoracic aorta efficiently provides large blood volumes, ideal for biochemical investigations. </p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147639282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel hairless highly immunodeficient mice model optimized for in vivo imaging.","authors":"Kouki Matsuda, Ryusho Kariya, Sawako Fujikawa, Kenji Maeda, Seiji Okada","doi":"10.1186/s42826-026-00275-9","DOIUrl":"10.1186/s42826-026-00275-9","url":null,"abstract":"<p><p>BACKGROUND: In vivo imaging is one of the analytical technologies that has been rapidly growing in demand in recent years to non-invasively observe the behavior of molecules such as genes and proteins in vivo and perform quantitative and qualitative analysis. Since the hair blocks and absorbs the fluorescence, Nude and Hairless mice have been used for in vivo imaging. Nude mice have been used for in vivo imaging for a long time; however, more efficient mice are needed for the next generation of imaging. RESULTS: We established a novel Hairless Rag2/Jak3 KO mice (Hairless R/J mice) model that exhibits hairlessness and a lack of T, B, and NK cell phenotypes. Hairless R/J mice exhibit thinner skin than that of Nude R/J mice. These mice also showed superior optical properties compared with Nude R/J mice, as demonstrated by green fluorescent beads and the mCherry-expressing human cholangiocarcinoma cell line M213, following subcutaneous transplantation. Furthermore, we show that Ihara cells, a human malignant melanoma cell line, can be used to facilitate live imaging of the growth of transplanted tumors. CONCLUSIONS: This novel mouse model will be a valuable tool for noninvasive tumor monitoring and evaluation of anticancer therapies. </p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13054980/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633892","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endovascular coil embolization compared to surgical ligation of the uterine artery in a non-human primate model in a model of preeclampsia.","authors":"A Makris, N Roshan, J Iliopoulos, R Waugh, A Al-Hindawi, B Corbett, C C Liu, B Lian, S Heffernan, J Hyett, J Ardui, S Pears, S A Karumanchi, A Hennessy","doi":"10.1186/s42826-026-00276-8","DOIUrl":"10.1186/s42826-026-00276-8","url":null,"abstract":"<p><strong>Background: </strong>Preeclampsia is a multisystem pregnancy disorder that is a major contributor to maternal and neonatal morbidity and mortality worldwide. An animal model facilitates further understanding of the disorder and allows for the investigation of targeted therapies prior to first in-human studies. Although there are several animal models for studying preeclampsia, the utero-placental ischemia (UPI) model is thought to best replicate the placental ischemia that develops as preeclampsia evolves in humans. An established non-human primate (NHP) model of UPI resembles human preeclampsia and has been important in understanding and finding cures for this disorder. To date the ischemia in most animal models has been undertaken by surgical ligation of a uterine artery. There are however alternate means to reduce arterial blood flow via endovascular means. This study aimed to investigate the difference between the surgical reduction in flow (surgical UPI) to the use of percutaneously delivered intra-arterial coils (coils UPI).</p><p><strong>Results: </strong>There was no significant difference between the two methods of inducing UPI for the measured parameters: circulating soluble fms-like tyrosine kinase-1(sFLT-1), blood pressure (BP) and proteinuria (p < 0.05). The results showed a percentage change from baseline in sFLT-1 of 3176 ± 1558% for the embolization and 2040 ± 1645% for the ligation group p = 0.11, three days post-UPI induction. Moreover, there was no difference between the two models in terms of preeclampsia-defining features.</p><p><strong>Conclusions: </strong>No statistically significant difference was found between the two methods, coils for endovascular embolization and surgical UPI. However, since endovascular embolization is a less invasive technique compared to ligation it may be preferred for induction of UPI in the NHP model.</p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13054988/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147633952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic kidney disease induces a distinct lipidomic signature and accelerates atherosclerosis progression in a novel minipig model.","authors":"Marcelino Bermúdez-López, Paula Nogales, Manuel Martí-Antonio, Eva Castro-Boqué, Virtudes M de Lamo, Laia Beà-Menchón, Sergio Luis-Lima, Esteban Porrini, Xavier Sanchez-Salguero, Mariona Jové, Elia Obis, Natàlia Mota-Martorell, Aurora Pérez-Gómez, Alicia Garcia-Carrasco, Milica Bozic, Jesús Guajardo, Carlos J Pérez-Sánchez, Serafí Cambray, Núria Amigó, Reinald Pamplona, Jacob F Bentzon, José M Valdivielso","doi":"10.1186/s42826-026-00274-w","DOIUrl":"10.1186/s42826-026-00274-w","url":null,"abstract":"<p><p>BACKGROUND: Chronic kidney disease (CKD) markedly accelerates atherosclerosis, driving the excess cardiovascular morbidity and mortality in these patients. While rodent models have been indispensable for mechanistic studies, their small vessel size limits the use of non-invasive vascular imaging to monitor disease progression. Therefore, large-animal models are needed to bridge experimental insights with clinical applicability. We aimed to establish a novel minipig model of CKD to characterize the temporal changes of CKD-driven atherosclerosis. Six female Yucatan minipigs overexpressing a human gain-of-function PCSK9 mutant were randomized to CKD (n = 3) or control (n = 3) groups. CKD was induced by selective clamping of the left renal artery branches combined with contralateral nephrectomy using a minimally invasive laparoscopic approach. All animals were fed with a high-fat, high-cholesterol diet and followed for 15 months. Longitudinal assessments included vascular and renal ultrasound, computed tomography, plasma iohexol clearance for glomerular filtration rate (GFR) determination, and biochemical profiling of ions, cytokines, chemokines, and lipids, including advanced lipoprotein, lipidomic and fatty acid analyses. Between-groups differences were evaluated using effect sizes with 95% confidence intervals. RESULTS: CKD pigs exhibited a significant reduction in GFR and increased blood creatinine. They exhibited accelerated atherogenesis, reflected by enhanced progressive adventitial vasa vasorum neovascularization in both carotid and iliac arteries, a higher burden of arterial calcifications in abdominal aorta and iliac arteries and postmortem larger atherosclerotic plaques and calcified areas in coronary arteries. CKD also altered the systemic inflammation profile (elevated IL-1ra, IL-2, IL-4, IL-8, and IL-10), promoted a proatherogenic lipoprotein phenotype with triglyceride-enriched VLDL, LDL, and IDL particles, increased VLDL particle number, and reduced LDL particle size. Lipidomic analyses revealed increased circulating and renal palmitic acid and distinct lesion-specific fatty acid signatures. Fatty streaks were enriched in palmitic acid and 10,16-dihydroxy-palmitic acid, and mature carotid plaques accumulated polyunsaturated fatty acids. CONCLUSIONS: This minimally invasive CKD model in gentically modified minipigs accelerates atherosclerosis and induces a unique lipidomic remodelling, providing a valuable translational platform to study kidney-vascular interactions and to test therapeutic interventions targeting CKD-driven atherosclerosis. </p>","PeriodicalId":17993,"journal":{"name":"Laboratory Animal Research","volume":"42 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2026-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13045143/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147609369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}