Laboratory animal science最新文献_第3页

Rescue of (NZB x NZW) F1 mice from oxygen-derived free radical injury by use of phosphatidylcholine-modified superoxide dismutase. 磷脂酰胆碱修饰的超氧化物歧化酶对(NZB x NZW) F1小鼠氧源性自由基损伤的修复作用。

Laboratory animal science Pub Date : 1999-10-01

M Nishikawa, R Igarashi, T Nakazawa, E Aikawa

引用次数: 0

Enteric lesions in SCID mice infected with "Helicobacter typhlonicus," a novel urease-negative Helicobacter species. 感染“伤寒螺杆菌”的SCID小鼠肠道病变，这是一种新的脲酶阴性螺杆菌。

Laboratory animal science Pub Date : 1999-10-01

C L Franklin, L K Riley, R S Livingston, C S Beckwith, R R Hook, C L Besch-Williford, R Hunziker, P L Gorelick

{"title":"Enteric lesions in SCID mice infected with \"Helicobacter typhlonicus,\" a novel urease-negative Helicobacter species.","authors":"C L Franklin, L K Riley, R S Livingston, C S Beckwith, R R Hook, C L Besch-Williford, R Hunziker, P L Gorelick","doi":"","DOIUrl":"","url":null,"abstract":"Background and purpose: Several rodent helicobacters have been associated with chronic active hepatitis or inflammatory bowel disease. Severe combined immunodeficient (SCID) mice appear to be inherently susceptible to disease attributable to these emerging pathogens. With the advent of polymerase chain reaction (PCR) analysis, it has become clear that several as yet unidentified Helicobacter species may also colonize rodents, but their capacity to cause disease is unknown.Methods: A Helicobacter species isolated from feces of a BALB/c mouse and provisionally named \"H. typhlonicus\" was used to inoculate helicobacter-free 4-week-old SCID mice (n = 11 males and 11 females). At various weeks after inoculation, mice were sacrificed and liver and intestinal specimens were collected for histologic examination and PCR analyses.Results: The C.B-17 scid/scid mice inoculated with \"H. typhlonicus\" developed moderate to severe proliferative typhlocolitis, similar to that seen in SCID mice infected with H. hepaticus or H. bilis. However, in contrast to mice infected with H. hepaticus or H. bilis, lesions of chronic active hepatitis were not detected in mice inoculated with \"H. typhlonicus.\" A similar disease syndrome developed in SCID mice cohabitated with B6D2F1 mice naturally infected with a novel Helicobacter species that was genetically identical to \"H. typhlonicus.\"Conclusion: \"Helicobacter typhlonicus\" joins a growing list of helicobacters that are capable of inducing enteric disease in immunodeficient mice.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"496-505"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reverse transcriptase polymerase chain reaction-based diagnosis and molecular characterization of a new rat coronavirus strain. 基于逆转录酶聚合酶链反应的大鼠冠状病毒新毒株诊断及分子特征分析。

Laboratory animal science Pub Date : 1999-10-01

S R Compton, B E Vivas-Gonzalez, J D Macy

{"title":"Reverse transcriptase polymerase chain reaction-based diagnosis and molecular characterization of a new rat coronavirus strain.","authors":"S R Compton, B E Vivas-Gonzalez, J D Macy","doi":"","DOIUrl":"","url":null,"abstract":"Background and purpose: Rat coronaviruses (RCV) are highly infectious and spread rapidly through laboratory rat colonies, causing sneezing, nasal and ocular discharges, photophobia, and cervical swelling. Current diagnostic methods include serologic testing and histologic examination. During a recent rat coronavirus outbreak, we tested a rapid, noninvasive method of RCV diagnosis that involved use of reverse transcriptase-polymerase chain reaction (RT-PCR) analysis to detect RCV RNA on cages housing infected rats.Methods: The RT-PCR was used to detect RCV RNA in tissues from infected rats and on cages housing infected rats and to amplify portions of the RCV N, M, and S genes for molecular characterization.Results: The RT-PCR detected RCV RNA on cages and in tissues from infected rats. The RCV-NJ N gene is most closely related to the MHV-Y N gene. The M proteins of RCV-NJ and RCV-SDA are 99% homologous, and the six RCV S protein fragments are 97 to 100% homologous.Conclusions: Use of RT-PCR with cage-swab specimens was capable of diagnosing RCV infection in and viral excretion from rats. Additionally, molecular characterization of the N, M, and S genes of RCV-NJ provided baseline information that can be used in performing further epidemiologic studies.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"506-13"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transdiaphragmatic lymphatic transport of intraperitoneally administered marker in hamsters. 仓鼠腹腔内给药标记物的横膈膜淋巴运输。

Laboratory animal science Pub Date : 1999-10-01

D L Fritz, D M Waag

{"title":"Transdiaphragmatic lymphatic transport of intraperitoneally administered marker in hamsters.","authors":"D L Fritz, D M Waag","doi":"","DOIUrl":"","url":null,"abstract":"Objective: To determine whether transdiaphragmatic transport in hamsters is similar to that described in other animals by examining transport of an intraperitoneally administered marker.Methods: Monastral blue B suspension was administered intraperitoneally to 28 male Syrian hamsters (Mesocricetus auratus). Four hamsters each were euthanized 7, 15, and 30 min, and 1, 2, 3, and 24 h later. Specimens were examined microscopically for presence of marker.Results: Marker was present in intrathoracic lymphatic vessels and cranial and caudal mediastinal lymph nodes by 7 min after its administration. The amount of marker in lymph nodes increased with time. The subcapsular distribution of marker was consistent with lymphatic transport. By 1 h after its administration, marker was present in the liver, spleen, bone marrow, and mesenteric and mandibular lymph nodes. Patterns of marker distribution in these tissues were consistent with hematogenous transport, but the amount of marker was considerably less than that in the intrathoracic lymph nodes at corresponding times.Conclusions: Particulates were most likely translocated from the hamster peritoneal cavity to intrathoracic lymph nodes via transdiaphragmatic lymphatic vessels. A portion of the translocated particulates entered the blood, where they were distributed to a variety of tissues within a short time.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"522-9"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved detection of Coxiella burnetii (Q fever) in livestock tissue through silica matrix DNA extraction and high-sensitivity PCR. 利用二氧化硅基质DNA提取和高灵敏度PCR技术改进了家畜组织中伯纳氏杆菌(Q热)的检测方法。

Laboratory animal science Pub Date : 1999-10-01

R W Ermel

引用次数: 0

Macfarlane Burnet centenary symposium on immunology and virology 3-5 August, 1999, Melbourne, Australia. 麦克法兰·伯内特免疫学与病毒学百年研讨会，1999年8月3-5日，澳大利亚墨尔本。

Laboratory animal science Pub Date : 1999-10-01

A L Smith

引用次数: 0

Characterization of rabbit Pasteurella multocida isolates by use of whole-cell, outer-membrane, and polymerase chain reaction typing. 利用全细胞、外膜和聚合酶链反应分型鉴定兔多杀性巴氏杆菌分离株。

Laboratory animal science Pub Date : 1999-10-01

S M Dabo, A W Confer, M Montelongo, Y S Lu

{"title":"Characterization of rabbit Pasteurella multocida isolates by use of whole-cell, outer-membrane, and polymerase chain reaction typing.","authors":"S M Dabo, A W Confer, M Montelongo, Y S Lu","doi":"","DOIUrl":"","url":null,"abstract":"Purpose: To characterize Pasteurella multocida isolates from laboratory rabbits using serotyping, sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins (WCPs) and outer-membrane proteins (OMPs), and polymerase chain reaction (PCR) fingerprinting.Methods: Fifty isolates were obtained from five sources: ATCC (1), Oklahoma (4), Michigan (9), Minnesota (7), and Texas (29). The PCR fingerprinting was conducted using two minisatellite probes for M13 and a modified M13 core sequence and two microsatellite probes--(GTG)5 and (GACA)4.Results: Forty-five isolates were serogroup A, and five were serogroup D. Ten WCP patterns (W1-W10) with one variation (W1a) and 10 OMP (OM1-OM10) patterns were found. Primers M13 phage, modified M13 phage, (GTG)5, and (GACA)4 generated 7, 9, 5, and 9 fingerprint types, respectively. Combination of WCP, OMP, and PCR fingerprint results yielded 39 groups with a discrimination index of 0.98. The PCR fingerprint results generally indicated clonal association among isolates within geographic locations except for the isolates from Texas, which varied markedly in PCR fingerprint types.Conclusion: Single primer PCR fingerprinting provided a simple and rapid means of typing P. multocida isolates from laboratory rabbits. Combinations of conventional and molecular typing enhanced differentiation among P. multocida isolated from rabbits with pasteurellosis.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"551-9"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of surrogate markers of impending death in the galactosamine-sensitized murine model of bacterial endotoxemia. 半乳糖胺致敏小鼠细菌性内毒素血症模型中濒死替代标志物的评价。

Laboratory animal science Pub Date : 1999-10-01

A Krarup, P Chattopadhyay, A K Bhattacharjee, J R Burge, G R Ruble

{"title":"Evaluation of surrogate markers of impending death in the galactosamine-sensitized murine model of bacterial endotoxemia.","authors":"A Krarup, P Chattopadhyay, A K Bhattacharjee, J R Burge, G R Ruble","doi":"","DOIUrl":"","url":null,"abstract":"Background and purpose: When evaluating vaccines for efficacy against gram-negative endotoxemia, the challenge has historically required death of a large percentage of test subjects. We attempted to identify surrogate markers of impending death to allow for early euthanasia without interfering with experimental data collection.Methods: Galactosamine-sensitized mice (n = 140) were inoculated intraperitoneally with various dosages of endotoxin, and development of clinical signs of disease--body temperature, body weight, hunched posture, ruffled coat, inability to ambulate, and loss of consciousness--was evaluated.Results: Wide fluctuations in body temperature (+/- 4 degrees C) were observed in survivors and nonsurvivors. Posture, coat, and body weight were not accurate predictors of death. Only inability to ambulate, with a positive predictive value of 100% (11 of 11), accurately predicted death in the experimental mice of this study.Conclusion: Using this surrogate marker, loss of ability to ambulate, 11 of 13 mice that developed this sign could have been euthanized early, preventing anywhere from 2 to 22 h of potential distress prior to death.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"545-50"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proliferative retinal changes in diabetic rats (WBN/Kob). 糖尿病大鼠视网膜增生性变化(WBN/Kob)。

Laboratory animal science Pub Date : 1999-10-01

T Matsuura, K Horikiri, K Ozaki, I Narama

引用次数: 0

Comparison of adjuvants with Leishmania antigens in a guinea pig model to induce delayed-type hypersensitivity responses. 佐剂与利什曼原虫抗原在豚鼠模型中诱导延迟型超敏反应的比较。

Laboratory animal science Pub Date : 1999-10-01

E J Briand, G R Ruble, J Stiteler, L D Harris, J R Burge, E T Soranaka, G Glenn, F Quance-Fitch, E D Rowton

{"title":"Comparison of adjuvants with Leishmania antigens in a guinea pig model to induce delayed-type hypersensitivity responses.","authors":"E J Briand, G R Ruble, J Stiteler, L D Harris, J R Burge, E T Soranaka, G Glenn, F Quance-Fitch, E D Rowton","doi":"","DOIUrl":"","url":null,"abstract":"Background and purpose: Guinea pigs have been a traditional model for studies of delayed-type hypersensitivity. They are the natural host of Leishmania enriettii and have been experimentally infected with other species of Leishmania. They have been used as a skin-test model to screen potential antigens for use in diagnostic tests for Leishmania. Use of complete Freund's adjuvant (CFA), along with whole promastigote Leishmania antigen, was necessary to sensitize guinea pigs to invoke a sufficient cell-mediated immune response. However, use of CFA has come under scrutiny by Animal Care and Use Committees due to the pathologic changes associated with its use.Methods: Thirty-two specific-pathogen-free male Hartley guinea pigs were inoculated with Leishmania antigens alone or mixed with one of three adjuvants (CFA, TiterMax, and liposomes), and were skin tested 2 weeks later.Results: For the Leishmania antigens tested, guinea pigs that received liposomes as an adjuvant had skin-test responses comparable to those of guinea pigs that received CFA. TiterMax was also tested, but cellular responses at antigen test sites were poor.Conclusions: Liposomes can be used in this model as a safe, effective adjuvant.","PeriodicalId":17937,"journal":{"name":"Laboratory animal science","volume":"49 5","pages":"519-21"},"PeriodicalIF":0.0,"publicationDate":"1999-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"21412902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0