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Leafspot Disease Progression and Components of Resistance Among Selected Peanut Genotypes in Ghana 加纳选定花生基因型的叶斑病进展及其抗性成分
IF 5
Legume Science Pub Date : 2026-02-19 DOI: 10.1002/leg3.70085
Isaac Boatey Akpatsu, Theophilus Kwabla Tengey, Frederick Kankam, Richard Oteng-Frimpong, Mark D. Burow
{"title":"Leafspot Disease Progression and Components of Resistance Among Selected Peanut Genotypes in Ghana","authors":"Isaac Boatey Akpatsu,&nbsp;Theophilus Kwabla Tengey,&nbsp;Frederick Kankam,&nbsp;Richard Oteng-Frimpong,&nbsp;Mark D. Burow","doi":"10.1002/leg3.70085","DOIUrl":"https://doi.org/10.1002/leg3.70085","url":null,"abstract":"<p>Early and late leafspot diseases are major constraints in peanut production, causing up to 70% yield losses in combined occurrences. Phenotyping has focused mainly on disease incidence and severity with little attention on disease progression and component traits that determine overall resistance. This study distinguished resistant and susceptible genotypes based on disease progression and components of resistance. Ten peanut genotypes were evaluated under natural leafspot pressure at Nyankpala, Ghana, over two cropping seasons. The set included six genotypes derived from crosses between BC<sub>3</sub>F<sub>6</sub> interspecific introgression lines and Spanish peanut genotypes and four released varieties. Disease incidence, severity, lesion number and diameter, and percentage of necrotic area were recorded to compute area under disease progress curve (AUDPC) and residence indices. Results revealed that leafspot disease progressed steadily among susceptible peanut genotypes as compared with the resistant genotypes. Susceptible genotypes exhibited higher lesion number, lesion diameter, and percentage necrotic area, which showed strong positive correlations with AUDPC-ELS, AUDPC-LLS, and AUDPC-DI. L076J had the highest resistance based on components of resistance index, whereas Nkatiesari had the highest level of resistance based on the disease progression index (DPI). Integrating both components into an overall resistance index (ORI) classified Nkatiesari and Sarinut-1 as resistant; L076J, L027B, and L010A1 as moderately resistant; L030, L046, and L104B as susceptible; and Sarinut-2 and Chinese as highly susceptible. These resistant genotypes provide valuable sources for leafspot management and peanut improvement in sub-Saharan Africa.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70085","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147320833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Exopolysaccharide-Based Microencapsulation of Plant Growth-Promoting Bacillus Strains Improves Germination, Growth, and Yield of Chilean Common Bean Cultivars 植物促生长芽孢杆菌胞外多糖微胶囊化提高智利普通豆品种的萌发、生长和产量
IF 5
Legume Science Pub Date : 2026-02-18 DOI: 10.1002/leg3.70083
Cynthia Meza, Antonio Fernandez-Barbero, Basilio Carrasco, José Mesquita-Neto, Aparna Banerjee
{"title":"Exopolysaccharide-Based Microencapsulation of Plant Growth-Promoting Bacillus Strains Improves Germination, Growth, and Yield of Chilean Common Bean Cultivars","authors":"Cynthia Meza,&nbsp;Antonio Fernandez-Barbero,&nbsp;Basilio Carrasco,&nbsp;José Mesquita-Neto,&nbsp;Aparna Banerjee","doi":"10.1002/leg3.70083","DOIUrl":"https://doi.org/10.1002/leg3.70083","url":null,"abstract":"<p>Microbial biostimulants offer sustainable alternatives to chemical fertilizers in legume cultivation by enhancing seed germination, nutrient uptake, and stress resilience, while maintaining the yield. However, their practical application is often limited by weak shelf life and reduced viability under environmental conditions. In this study, we develop a composite hydrogel encapsulation system composed of alginate, chitosan, and bacterial exopolysaccharides (EPSs) to improve the viability and functionality of two plant growth-promoting bacteria (PGPB): <i>Bacillus proteolyticus</i> Cyn1 and <i>Bacillus safensis</i> Cyn2. These strains, originally isolated from Chilean common bean rhizosphere, were characterized for growth dynamics and morphological traits using optical density monitoring and scanning electron microscopy. The encapsulated formulations were tested for cell viability under 30-day storage time and evaluated for their growth stimulation efficacy on three Chilean cultivars of <i>Phaseolus vulgaris</i> L. (“Zorzal,” “Sapito,” and “Mantequilla”) under in vitro germination, controlled growth condition, and open-field condition. Results demonstrated composite hydrogel (alginate–chitosan–EPS) matrix significantly enhanced bacterial viability compared to single-layer capsules. Confocal microscopy with live/dead staining confirmed sustained cell viability (~70% after 30 days). Seed germination assays revealed improvements in total plant height, plumule and radicle length, root number, and germination rate in biostimulant-treated groups, with the “Mantequilla” cultivar showing the most noticeable response. The biostimulant enhanced early growth and physiological performance of common bean cultivars in a genotype-dependent manner, increasing plant height, biomass accumulation, leaf number, and chlorophyll a content, with the strongest responses observed in “Zorzal” and “Sapito,” as supported by multivariate analyses. Importantly, these early physiological advantages translated into improved agronomic performance under field conditions with increases in pod and seed number, biomass, and final yield across cultivars. This study highlights the effectiveness of EPS-based composite encapsulation systems in improving stability and field performance, offering a promising strategy for sustainable enhancement of legume crop performance in a changing world.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70083","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147315512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritional Composition, Phenolic Content, and Antioxidant Assessment of Mediterranean Lupinus Seeds 地中海Lupinus种子的营养成分、酚类含量及抗氧化性评价
IF 5
Legume Science Pub Date : 2026-02-18 DOI: 10.1002/leg3.70082
Yosr Ben Mhara, Hela Belhaj Ltaeif, Anis Sakhraoui, Raouia Ben Rhouma, Balkis Bessouda, Slim Rouz
{"title":"Nutritional Composition, Phenolic Content, and Antioxidant Assessment of Mediterranean Lupinus Seeds","authors":"Yosr Ben Mhara,&nbsp;Hela Belhaj Ltaeif,&nbsp;Anis Sakhraoui,&nbsp;Raouia Ben Rhouma,&nbsp;Balkis Bessouda,&nbsp;Slim Rouz","doi":"10.1002/leg3.70082","DOIUrl":"https://doi.org/10.1002/leg3.70082","url":null,"abstract":"<p>This study presents a comprehensive biochemical characterization and antioxidant profiling of four Mediterranean lupin species, namely <i>Lupinus albus</i> L., <i>Lupinus luteus</i> L., <i>Lupinus cosentinii Guss</i>., and <i>Lupinus gussoneanus</i> J. Agardh, to assess their potential as sustainable functional ingredients. The nutritional composition, mineral content, phenolic compounds, and in vitro antioxidant activities (DPPH, ABTS, FRAP) were evaluated. Significant interspecific variations were observed: <i>L. luteus</i> exhibited the highest crude protein content (35.45% DM), while <i>L. cosentinii</i> was particularly rich in crude cellulose (20.77% DM). <i>L. albus</i> showed the highest gross energy value (4487.3 kcal kg<sup>−1</sup> DM). Mineral analysis revealed species-specific accumulations, particularly of phosphorus and magnesium in <i>L. luteus</i>. Furthermore, <i>L. gussoneanus</i> possessed the highest total polyphenol content (8.41 mg GAE g<sup>−1</sup> DM), whereas <i>L. albus</i> had the highest condensed tannins (0.97 mg CE g<sup>−1</sup> DM). Antioxidant activity was highly method-dependent, with ABTS values strongly correlating with total polyphenols (<i>r</i> = 0.973, <i>p</i> &lt; 0.001). Notably, <i>L. gussoneanus</i> showed exceptional ABTS activity (21.93 μmol TE g<sup>−1</sup> DM). The results highlight the influence of phytochemical composition on bioactivity and underscore the need for multi-method antioxidant assessment. These findings support the valorization of Mediterranean lupins as promising components for functional foods and animal feed formulations, contributing to nutritional sustainability and improved health outcomes.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70082","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147320888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cultivar-Specific and Field Site–Specific Protein and Metabolite Patterns of Faba Bean (Vicia faba L.) Seeds 蚕豆(Vicia Faba L.)的品种特异性和田间特异性蛋白质及代谢模式种子
IF 5
Legume Science Pub Date : 2026-02-15 DOI: 10.1002/leg3.70084
Julia Brandt, Alexandra Hüsken, Eileen Whitelaw, Georg Langenkämper, Rabea Schweiger
{"title":"Cultivar-Specific and Field Site–Specific Protein and Metabolite Patterns of Faba Bean (Vicia faba L.) Seeds","authors":"Julia Brandt,&nbsp;Alexandra Hüsken,&nbsp;Eileen Whitelaw,&nbsp;Georg Langenkämper,&nbsp;Rabea Schweiger","doi":"10.1002/leg3.70084","DOIUrl":"https://doi.org/10.1002/leg3.70084","url":null,"abstract":"<p>Sustainable agriculture is an important component of current and future food production, particularly in the face of the rising human world population. In this context, faba bean (<i>Vicia faba</i> L.) is highly valuable due to its capability to enrich soils with nitrogen and because its seeds have a high nutritional value. In this study, we compared the seed chemistry of 11 faba bean cultivars grown at 13 field sites in Germany. Nitrogen and sulfur contents were determined with the Dumas method, and protein contents were calculated based on the obtained nitrogen data. SDS-PAGE was used to analyze proteins, whereas metabolic fingerprinting was applied to screen (semi-)polar metabolites, mainly comprising specialized metabolites. The protein content differed predominantly between cultivars but also between field sites. The sulfur content strongly depended on the field site. The patterns of proteins and metabolites were largely cultivar-specific. Additionally, the number of metabolic features varied somewhat between field sites. Our study suggests that breeding led to comprehensive changes in the seed chemistry of <i>V. faba</i> bean, going far beyond protein content and certain metabolites targeted in breeding. The rather high consistency when comparing field sites indicates that breeding has led to more or less stable cultivars. Future studies should place more emphasis on far-reaching effects of breeding on the seed proteome and metabolome of faba bean, as various proteins and metabolites in concert determine the nutritional quality of seeds of this important crop species.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70084","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147320762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
One-Step ex Vitro Hairy Root Transformation of Soybean Using the RUBY Visible Reporter Gene 利用RUBY可见报告基因一步离体转化大豆毛状根
IF 5
Legume Science Pub Date : 2026-02-15 DOI: 10.1002/leg3.70081
Mohsen Niazian, François Belzile, Shaun J. Curtin, Davoud Torkamaneh
{"title":"One-Step ex Vitro Hairy Root Transformation of Soybean Using the RUBY Visible Reporter Gene","authors":"Mohsen Niazian,&nbsp;François Belzile,&nbsp;Shaun J. Curtin,&nbsp;Davoud Torkamaneh","doi":"10.1002/leg3.70081","DOIUrl":"https://doi.org/10.1002/leg3.70081","url":null,"abstract":"<p><i>Rhizobium rhizogenes</i> (formerly <i>Agrobacterium rhizogenes</i>)-mediated hairy root transformation is one of the most efficient genetic engineering methods in soybean. In this study, we explored the feasibility of using the <i>RUBY</i> reporter gene in a one-step <i>ex vitro</i> (hypocotyl cutting) transformation protocol in soybean. The inoculated seedlings were cultivated in growth pouches, facilitating easy monitoring of transformation efficiency and visualization of the resulting red transgenic roots. The <i>R. rhizogenes</i> strains R1000, ARqual, and K599 demonstrated a 100% hairy root induction in four soybean genotypes (20SS01, Bert, Jack, and Williams). The highest transformation efficiency (78.33%) was obtained by inoculation of 7-d-old seedlings of <i>cv</i>. Bert with the R1000 strain. This streamlined method offers a rapid and cost-effective means for real-time, high-throughput phenotyping of transgenic roots. The utilization of the <i>RUBY</i> reporter gene in conjunction with <i>ex vitro</i> hairy root transformation represents a promising avenue to accelerate in-root genetic modifications in soybeans.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147320763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Efficiency of Maize and Common Bean Intercropping Using Economic, Competitive, and Biological Indices for a Sustainable Production 利用经济、竞争和生物指标分析玉米与普通豆间作的可持续生产效率
IF 5
Legume Science Pub Date : 2026-01-29 DOI: 10.1002/leg3.70072
Ebrahim Zolfagari Kotbehsara, Peyman Sharifi, Mohammad Hossein Ansari
{"title":"The Efficiency of Maize and Common Bean Intercropping Using Economic, Competitive, and Biological Indices for a Sustainable Production","authors":"Ebrahim Zolfagari Kotbehsara,&nbsp;Peyman Sharifi,&nbsp;Mohammad Hossein Ansari","doi":"10.1002/leg3.70072","DOIUrl":"https://doi.org/10.1002/leg3.70072","url":null,"abstract":"<p>Intercropping is an effective method for resource utilization that typically enhances crop yields compared with monoculture. This study was conducted using split plot in a randomized complete block design with three replications over the 2022–2023 years. The main plot was designated for inoculation with <i>Rhizobium phaseoli</i> at two levels (inoculation and non-inoculation), while the subplot was assigned to the maize-bean intercropping ratio at five levels: 100% maize, 100% bean, 75% maize +25% bean (M75B25), 50% maize +50% bean (M50B50), and 25% maize +75% bean (M25B75). Total land equivalent ratio (LER) increased with a higher proportion of bean in intercropping, and the highest LER was achieved in the M25B75 ratio in both years. The monetary advantage index (MAI) was greater for M25B75 compared with the other two intercropping ratios. The average tester view of biplot revealed that the M25B75 intercropping ratio under inoculation conditions resulted in the most pronounced expression of the studied traits in both maize and bean. M25B75 was the most effective for enhancing maize and bean traits in both inoculation and non-inoculation conditions. These results indicate that cultivating a combination of bean and maize with <i>rhizobium</i> bacteria inoculation can effectively enhance the yield of both crops.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146139952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Gene Editing and Transformation Strategies for Engineering Drought-Tolerant Legumes 工程抗旱豆科植物的基因编辑与转化策略
IF 5
Legume Science Pub Date : 2026-01-27 DOI: 10.1002/leg3.70080
Andrea Fernandez-Gutierrez, Shaun J. Curtin, Juan J. Gutierrez-Gonzalez
{"title":"Gene Editing and Transformation Strategies for Engineering Drought-Tolerant Legumes","authors":"Andrea Fernandez-Gutierrez,&nbsp;Shaun J. Curtin,&nbsp;Juan J. Gutierrez-Gonzalez","doi":"10.1002/leg3.70080","DOIUrl":"https://doi.org/10.1002/leg3.70080","url":null,"abstract":"<p>Legumes play a vital role in agriculture, nutrition, and the economy, but their production faces significant threats. Among these, drought and its unpredictability will be the most damaging constraint in the coming decades. Enhancing drought tolerance is essential for resilient and sustainable legume cultivation, and genetic engineering through gene modification or editing offers promising solutions. Long-term drought tolerance involves regulating molecular pathways, such as the ABA-dependent or independent mechanisms, which control the expression of stress-related genes, making them ideal targets for genetic optimization. However, many legume crop genotypes are difficult to transform due to low transformation or regeneration efficiency. Recent research has therefore focused on both identifying key genes for modification and improving transformation and regeneration techniques. This review examines recent advancements in legume transformation methods and the genetic modifications aimed at increasing drought resilience in legume crops.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70080","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146155136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Explorative Study of the Effect of Extrusion of Black Bean Germinated-Dehulled Cotyledon on Gut Microbiota 黑豆发芽去皮子叶挤压对肠道菌群影响的探索性研究
IF 5
Legume Science Pub Date : 2026-01-20 DOI: 10.1002/leg3.70078
Arlette Santacruz, Carlos Martínez-Vitela, Erick Heredia-Olea, Sebastian Gradilla-Hernández, Marisela González-Ávila, Esther Pérez-Carrillo, Janet A. Gutiérrez-Uribe
{"title":"Explorative Study of the Effect of Extrusion of Black Bean Germinated-Dehulled Cotyledon on Gut Microbiota","authors":"Arlette Santacruz,&nbsp;Carlos Martínez-Vitela,&nbsp;Erick Heredia-Olea,&nbsp;Sebastian Gradilla-Hernández,&nbsp;Marisela González-Ávila,&nbsp;Esther Pérez-Carrillo,&nbsp;Janet A. Gutiérrez-Uribe","doi":"10.1002/leg3.70078","DOIUrl":"https://doi.org/10.1002/leg3.70078","url":null,"abstract":"<p>Sequential processing, germination-dehulling-extrusion, improves technofunctional and chemical properties of black bean cotyledons. This work aims to evaluate the effect of germinated, dehulled and extruded black bean cotyledon on <i>Lactobacillus</i> and <i>Bifidobacteriu</i>m growth using the Automated and Robotic Intestinal System (ARIS). Germinated-dehulled cotyledons were thermoextruded at 4 last barrel temperatures: 95°C (E1), 120°C (E2), 135°C (E3), and 145°C (E4). Proximal composition, soluble and insoluble dietary fiber, and starch content were determined. All flours were evaluated as carbon and nitrogen sources for acid lactic fermentation. All the flours obtained from the extrudates contained higher soluble dietary fiber than cooked beans and that obtained at 120°C (E2) had 12.89% while cooked beans had 2.17%. E2 proved to be the most effective medium made from experimental materials in acid lactic fermentation, achieving the same ending microbial concentration as positive controls (standard MRS broth or its homemade equivalent). E2 flour ARIS evaluation showed bifidogenic properties, increasing content 70% after 8 days of testing. The sequential processing of black bean generated a new food product alternative that has bifidogenic properties and could be consumed as a snack.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70078","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146091205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A Pathway-Based Metabolomics Approach Reveals Metabolic Strategies and Reprogramming in Drought-Tolerant Soybean Genotypes 基于途径的代谢组学方法揭示了耐旱大豆基因型的代谢策略和重编程
IF 5
Legume Science Pub Date : 2026-01-20 DOI: 10.1002/leg3.70079
Namhee Lee, Donna K. Harris, Zenglu Li, Joon Hyuk Suh
{"title":"A Pathway-Based Metabolomics Approach Reveals Metabolic Strategies and Reprogramming in Drought-Tolerant Soybean Genotypes","authors":"Namhee Lee,&nbsp;Donna K. Harris,&nbsp;Zenglu Li,&nbsp;Joon Hyuk Suh","doi":"10.1002/leg3.70079","DOIUrl":"https://doi.org/10.1002/leg3.70079","url":null,"abstract":"<p>Soybean (<i>Glycine max</i>) is a globally important crop, yet its productivity is highly susceptible to drought stress, particularly in rainfed systems. To better understand the biochemical basis of drought tolerance, this study employed pathway-based metabolomics to characterize differentially expressed metabolites between drought-tolerant and susceptible soybean genotypes and to identify underlying mechanisms. Metabolite profiles of leaves collected from both watered and drought-stressed conditions were analyzed using liquid chromatography–mass spectrometry. Discriminant metabolites were identified through multivariate statistical analysis and pathway mapping. Principal component analysis revealed distinct metabolic variation primarily under drought stress, indicating that drought-tolerant genotypes engage in active metabolic reprogramming in response to water deficit. Overall, the metabolic responses of drought-tolerant genotypes were less pronounced than those of susceptible ones, suggesting a more selectively managed allocation of metabolic resources in the tolerant genotypes. Pathway analysis indicated that tolerant genotypes selectively enhanced specific primary and secondary metabolic processes, including central carbon metabolism, shikimate pathway–associated metabolites, and specific amino acid pools, while also displaying divergent allocation within phenylpropanoid (secondary metabolism) and branched-chain amino acid pathways (primary metabolism). Both tolerant and susceptible cultivars exhibited shared drought responses, including hormonal activation, lipid remodeling, accumulation of phenylpropanoid intermediates, and osmoprotective amino acids. This study demonstrates that drought adaptation arises from the interplay between conserved biochemical adjustments and genotype-specific reprogramming in primary and secondary metabolism, providing metabolite-level insights that can guide future large-scale field studies aimed at selecting genotypes for drought tolerance using biomarkers.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70079","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146091207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Influence of Dry and Steam Thermal Pretreatments on the Structural Properties and Flowability of Faba Bean Flours 干燥和蒸汽热处理对蚕豆粉结构性能和流动性的影响
IF 5
Legume Science Pub Date : 2026-01-08 DOI: 10.1002/leg3.70075
Abraham Badjona, Robert Bradshaw, Caroline Millman, Martin Howarth, Bipro Dubey
{"title":"Influence of Dry and Steam Thermal Pretreatments on the Structural Properties and Flowability of Faba Bean Flours","authors":"Abraham Badjona,&nbsp;Robert Bradshaw,&nbsp;Caroline Millman,&nbsp;Martin Howarth,&nbsp;Bipro Dubey","doi":"10.1002/leg3.70075","DOIUrl":"https://doi.org/10.1002/leg3.70075","url":null,"abstract":"<p>Faba beans are emerging as a promising alternative protein source for bakeries, snacks, cereals and ready-to-eat foods. The quality and consistency of such products depend strongly on the processing behaviour of their flours, including handling, storage, mixing and extrusion performance, all of which are governed by flour flow and structural characteristics. However, there is currently a lack of information on the structural and flow properties of thermally treated faba bean flours. This study aims to understand the influence of dry and steam-heating temperature (70°C–100°C for 15–45 min) on the physical, structural and flowability properties of faba bean flours. Moisture content consistently decreased with high drying temperatures and longer duration for dry heating and vice versa for steam treatment. Dry-treated samples exhibited reduced particle size (D₅₀ = 14.5–18.9 μm), contributing to enhanced grindability but also increased cohesiveness. Fourier-transform infrared (FTIR) analysis revealed conformational changes in protein and starch domains, confirming that heating mode and moisture jointly influence molecular structure and powder behaviour. Flow indices (<i>ff</i><sub><i>c</i></sub>) classified dry-heated flours as cohesive to very cohesive (<i>ff</i><sub><i>c</i></sub> = 1.61–2.91), whereas steam-treated samples, particularly at 70°C for 15 min, were easy flowing (<i>ff</i><sub><i>c</i></sub> = 4.24). Principal component analysis explained 70.78% of the variance, distinguishing treatments based on moisture and flow attributes. These findings demonstrate that controlled thermal pretreatments can be used to tailor faba bean flour handling properties, facilitating their use in food manufacturing processes such as flour conveying, blending and hydration-based applications where powder flow and stability are critical.</p>","PeriodicalId":17929,"journal":{"name":"Legume Science","volume":"8 1","pages":""},"PeriodicalIF":5.0,"publicationDate":"2026-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/leg3.70075","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145963995","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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