Kuwait Journal of Science最新文献

{"title":"Stenotrophomonas maltophilia G17: Potential antifungal agent isolated from the gut of black soldier fly larvae against Ganoderma boninense","authors":"Iman Santoso ,&nbsp;Qonita Gina Fadhilah ,&nbsp;Andi Eko Maryanto ,&nbsp;Ratna Yuniati ,&nbsp;Aulia Sekar Putri ,&nbsp;Helmy Tamrela ,&nbsp;Ahmad Sugiyanto ,&nbsp;Ira M. Sigar","doi":"10.1016/j.kjs.2024.100309","DOIUrl":"10.1016/j.kjs.2024.100309","url":null,"abstract":"<div><p>Oil palm (<em>Elaeis guineensis</em> Jacq.) is the primary source of foreign exchange in Indonesia's plantation sector. However, these plants are very vulnerable to attack by <em>Ganoderma</em> spp., which causes basal stem rot (BSR) and upper stem rot (USR) disease, resulting in significant economic losses. In this study, antagonistic assays of the G17 isolate obtained from the gut of black soldier fly (BSF) larvae showed potential biocontrol against <em>G. boninense</em>. Furthermore, antibiosis assays showed that the <em>Stenotrophomonas maltophilia</em> G17 isolate inhibited the growth of <em>G. boninense</em> by up to 85.56%–91.98%. Molecular identification using 16S rRNA, reinforced by biochemical characterization tests, confirmed the G17 isolate as <em>S</em>. <em>maltophilia.</em> Growth test results showed that <em>S. maltophilia</em> G17 cannot grow at a temperature of 37 °C within 24 h. The isolate also exhibited negative hemolytic activity, indicating it is not a pathogenic bacterium. <em>S. maltophilia</em> G17 exhibited lytic enzymes such as proteolytic, lipolytic, and chitinolytic activity. Among them, chitinolytic enzymes might be involved in the biocontrol process.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100309"},"PeriodicalIF":1.2,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001342/pdfft?md5=218c951a3ff103cd4e05e9ad65175137&pid=1-s2.0-S2307410824001342-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141963030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modeling of sediment transport direction affecting Failaka island marina bathymetry, Kuwait","authors":"K. Al-Salem ,&nbsp;M. Al-Sarawi ,&nbsp;A. Al-Rashed","doi":"10.1016/j.kjs.2024.100308","DOIUrl":"10.1016/j.kjs.2024.100308","url":null,"abstract":"<div><p>Failaka Island has a marina, which was constructed in 1979 and managed by the Ministry of Communication (MOC) in collaboration with Touristic Enterprises Company (TEC). The marina is widely used for its ferry landing facility. The coastal waters around Failaka are turbid, and current velocities tend to be as high as ∼0.5 m/s during spring tide. The marina basin, with axial plane view and dimensions of about 285m by 260m, experiences shoaling of naturally flocculated fine (cohesive) sediment and coarse sand at the entrance of the channel and more generally in the southern half of the basin. Particularly noteworthy is its heavy shoaling zone along the inner side of the south breakwater close to the entrance. The Failaka island marina is suffering from siltation and sediment deposition issues. The study was carried out numerically to assess the annual sedimentation, sediment direction, and longshore current from different locations around the marina and recommend solutions to reduce the siltation and sediment deposition based on sediment transport direction at the marina entrance. The study shows that the sediment is mostly transported to the Failaka marina entrance from the southeast due to wave action. The cumulative sediment transport for the period 2016 to 2020 as depicted from locations 1 and 2, which are vulnerable to the southeast waves, is 9,164 m³ and 12,323 m³. The annual sediment transport direction affecting the marina is oriented from south to north due to wave action.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100308"},"PeriodicalIF":1.2,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001330/pdfft?md5=96611bc8ba2d46ca5d32e7f491dd2d52&pid=1-s2.0-S2307410824001330-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141848305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationships between site index modeling of crimean juniper and habitat factors","authors":"Emre Kuzugudenli ,&nbsp;Kursad Ozkan","doi":"10.1016/j.kjs.2024.100296","DOIUrl":"10.1016/j.kjs.2024.100296","url":null,"abstract":"<div><p>The aim of this study is to examine the relationships between height growth and some site factors of Crimean juniper in southwestern Anatolia. The data was collected from 130 sample plots selected from 10 districts in western Anatolia. The age and height of the Crimean juniper were measured in each sample plot. Surface soil samples collected from each sample plot were analyzed for pH, organic matter, carbonate content, texture, stoniness, available water capacity, wilting point, and field capacity. All site index values were calculated at a standard age of 100 years old. Spearman and Pearson correlation analyses were used to determine the relationships between plus tree and site factors. According to Spearman's correlation analysis, the site index showed a negative relationship with limestone. According to the results of the Pearson correlation analysis, the numerical variables LATTUD, SLOPE, SURSTON, and HINDEX had a 1% relationship with the site index, and RINDEX had a 5% relationship. Once the binary relationships had been determined, the multivariate relationships were modelled using generalized additive models. The best site index model was obtained using a generalized additive model 1 (R = 0.63), including latitude, longitude, altitude, slope, and stoniness.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100296"},"PeriodicalIF":1.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001214/pdfft?md5=b9c8d02d92cfd7b7220087398c29c8d8&pid=1-s2.0-S2307410824001214-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141953050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural modelling of subsurface geologic structures in Anambra and adjoining Bida Basins using aeromagnetic data: Implications for mineral explorations.","authors":"Ayatu Ojonugwa Usman ,&nbsp;Ema Michael Abraham ,&nbsp;Chukwudi Chris Ezeh ,&nbsp;George-Best Azuoko ,&nbsp;Augustine Ifeanyi Chinwuko ,&nbsp;Chima Joshua Chizoba ,&nbsp;Obinna Chigoziem Akakuru","doi":"10.1016/j.kjs.2024.100307","DOIUrl":"10.1016/j.kjs.2024.100307","url":null,"abstract":"<div><p>Aeromagnetic data over parts of the Northern Anambra and Southern Bida basins have been evaluated with emphasis on the subsurface geologic structures controlling mineralization and delineating the geomorphology of the basin. Four aeromagnetic data sheets (246 (Kabba), 247 (Lokoja), 266 (Auchi), and 267 (Idah)) were acquired through purchase, assemblage, analysis, and interpretation using integrated processing techniques of Euler deconvolution, analytical signal (AS), source parameter imaging (SPI), and 3D magnetic inversion model. The data are collected laterally in a series of NW-SE trajectories spaced 2 km apart, with an average flight altitude of approximately 150 m, and tielines occurring at approximately 20 km intervals. Qualitative observation of TMI and RAM shows that the research region is greatly fractured, with structures orienting in the E-W direction. The spectral analysis result shows that the sedimentary infillings range from 0.60 to 4.03 km. 3D model and curie isotherm depth subsurface maps reveal deeper curie depths at Kabba, Adavi, and Itakpe zones (28.0–40.0 km) and shallow curie depths at Auchi, Osara, Idah, and Anyigba areas (18.0–27.0 km). The result from the heat flow model shows an inverse relationship with the Curie isotherm depth. The contact depth sources calculated from Euler depth assessments show that the majority of these contact sources are trending in the E-W directions. The overall structural geomorphology of the research region conforms to the general pattern of structural trends within the research region. Most of the structures are trending NNE-SSW, NNW-SSE, and NE-SW, while the minor structures trend in the E-W direction. 3D inversion results show a discrete subsurface geologic structure that may house minerals within the region.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100307"},"PeriodicalIF":1.2,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001329/pdfft?md5=69fdedcc4a9b052cdc48298ed244c8f9&pid=1-s2.0-S2307410824001329-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141842512","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties and applications of the Tan Weibull loss distribution","authors":"John Abonongo","doi":"10.1016/j.kjs.2024.100304","DOIUrl":"10.1016/j.kjs.2024.100304","url":null,"abstract":"<div><p>Probability models play crucial role in modeling loss in the finance and actuarial sciences. In this article, a new family of loss distributions known as the Tan F-Loss family of distributions is proposed with the Tan Weibull Loss distribution as a special case. The density exhibits decreasing, right skewed, symmetric, and approximately symmetric shapes. The hazard rate function shows decreasing and modified bathtub shapes. The statistical properties of the Tan Weibull Loss distribution including the quantile function, moments, expansion of the general rate, moment generating function, incomplete moment, and order statistics are studied. The maximum likelihood estimators of the distribution are also studied. Simulations are carried out to examine the behavior of the estimators. The results show that the estimators are consistent. The usefulness of the proposed distribution is demonstrated with two insurance loss datasets. The results show that the proposed distribution gives a better parametric to the two datasets compared with the competing distributions.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100304"},"PeriodicalIF":1.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001299/pdfft?md5=712149dcf4c4643ea4052e6d298f5d30&pid=1-s2.0-S2307410824001299-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chitosan (CTS) induced secondary metabolite production in Canscora decussata Schult. - An endangered medicinal plant","authors":"Loganathan Kousalya ,&nbsp;Parthasarathy Seethapathy ,&nbsp;Deepu Pandita ,&nbsp;Sankaran Packiaraj ,&nbsp;Sakthivel Venkatesh ,&nbsp;Subbiah Sankaralingam ,&nbsp;Malaisamy Arunkumar ,&nbsp;Balasundaram Harinathan ,&nbsp;Anu Pandita ,&nbsp;Ryan Casini ,&nbsp;Eman A. Mahmoud ,&nbsp;Ihab Mohamed Moussa ,&nbsp;Hosam O. Elansary","doi":"10.1016/j.kjs.2024.100306","DOIUrl":"10.1016/j.kjs.2024.100306","url":null,"abstract":"<div><p>Bioengineering has excellent potential in plant-based <em>in vitro</em> cultures for efficiently synthesizing desired plant products. Due to the rapid destruction of natural habitats, <em>in vitro</em> methods might save endangered species. Winged-Stem Canscora is an endangered medicinal herb used in traditional medicine for brain disorders. This study investigates the induction of in-vitro metabolite production using chitosan. Among the various concentrations of auxins tested, 82% of callus was obtained from Murashige and Skoog (MS) medium with 3 mg L⁻¹ Kinetin (KIN) + 1 mg L⁻¹ 1-Naphthaleneacetic acid (NAA). The highest callus content was after the 3rd subculture at 200 mg L⁻¹ chitosan treatment. On the 70th day of culture, levels of total phenolics and flavonoids are increased in the elicited and normal callus. However, antioxidant activity was higher in elicited callus compared to normal callus and wild plants. Higher secondary metabolite concentrations of elicited callus have superior anti-bacterial and anti-fungal activity. Secondary metabolites from elicited callus have a lower inhibitory concentration than those from wild plant and normal callus. High-performance thin layer chromatography (HPTLC) analysis showed that the elicited callus had higher amounts of mangiferin (0.178 μg mL⁻¹) and scopoletin (0.133 μg mL⁻¹). An efficient approach was employed to ensure that sufficient amounts of the widely used plant secondary metabolites from medicinal plants, such as mangiferin and scopoletin, were accessible to meet demand.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100306"},"PeriodicalIF":1.2,"publicationDate":"2024-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001317/pdfft?md5=639cce48092d2996353c308112e27e63&pid=1-s2.0-S2307410824001317-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial, phytochemical, and antioxidant characterization of the leaf extracts of Clematis montana and Clematis grata","authors":"Ismat Nawaz ,&nbsp;Sarfaraz Nawaz ,&nbsp;Syed Tatheer Alam Naqvi ,&nbsp;Aimen Nasir ,&nbsp;Irum Shahzadi ,&nbsp;Raza Ahmed ,&nbsp;Ayesha Baig ,&nbsp;Mohammad Maroof Shah","doi":"10.1016/j.kjs.2024.100305","DOIUrl":"10.1016/j.kjs.2024.100305","url":null,"abstract":"<div><p>The worldwide emergence of multidrug-resistant bacterial infections among the population has impaired the existing effectiveness of antimicrobial treatments, necessitating the hunt for other alternatives. We aimed to find the antibacterial and antioxidant activities of <em>Clematis montana</em> and <em>C</em>. <em>grata</em> leaf extracts (both species have medicinal use among the local population). Extracts were assessed against four pathogenic bacterial strains (<em>Escherichia coli, Salmonella typhi</em>, <em>Pseudomonas aeruginosa,</em> and <em>Staphylococcus aureus</em>), through the agar well diffusion method. Total flavonoid content (TFC), total phenolic content (TPC), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity were quantified. Results suggested that all plant extracts, particularly those extracted with methanol, were potentially active in suppressing pathogenic bacterial growth (particularly against <em>P. aeruginosa</em> and <em>E. coli</em>) although their efficiency varied when compared to vancomycin. However, the antibacterial activity of methanolic extracts from both species was not greatly influenced by the method of extraction (maceration/soxhlet apparatus). TFC was significantly higher in the methanolic extracts of both species in comparison to the n-hexane extracts, whereas TPC and DPPH scavenging activity were detected to be highest in the methanolic extract from the leaves of <em>C. grata.</em> Our results suggested that the leaf extracts from both <em>Clematis</em> species, particularly the methanolic-based extract of <em>C. montana,</em> might serve as a promising source of an effective antibacterial agent.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100305"},"PeriodicalIF":1.2,"publicationDate":"2024-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001305/pdfft?md5=566e0ec0cfd1fe9c1d65682548432281&pid=1-s2.0-S2307410824001305-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141854006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An application of textual document classification for Arabic governmental correspondence","authors":"Khaled Alzamel,&nbsp;Manayer Alajmi","doi":"10.1016/j.kjs.2024.100299","DOIUrl":"10.1016/j.kjs.2024.100299","url":null,"abstract":"<div><p>The automation of classifying Arabic documents is becoming increasingly in demand, especially when dealing with an ever-growing amount of linguistic data. Natural language processing (NLP) has recently become one of the most significant fields in artificial intelligence (AI) thanks to recent advances in introducing transformer-based models. Transformers facilitate the use of reusable models by using pre-trained models (PTMs). This study aims to fine-tune monolingual (AraBERT (Antoun et al., 2020)), bilingual (GigaBERT (Lan et al., 2020)), and multilingual (XLM-RoBERTa (Conneau et al., 2020)) transformer-based encoder models to classify official Arabic correspondence in pre-defined classes and compare their predictive performance in terms of accuracy, using a new balanced dataset. The new balanced dataset has 22,741 Arabic texts and is categorized into six categories labeled with the most common ministries’ names. The results in this study show that GigaBERT achieved the highest accuracy rate of 98%. The implemented models may contribute to the domain of information systems (ISs) to facilitate the classification process in ministries without human intervention.</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100299"},"PeriodicalIF":1.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S230741082400124X/pdfft?md5=7773964c72c4b5c247bc08c319486326&pid=1-s2.0-S230741082400124X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141840039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation, path-coefficient and principal component analysis association among quantitative traits in strawberry to unlock potential of vertical farming system","authors":"Pallvi Verma ,&nbsp;Gurpreet Singh ,&nbsp;Shailesh Kumar Singh ,&nbsp;Manish Bakshi ,&nbsp;Anis Ahmad Mirza ,&nbsp;Anmol ,&nbsp;Suhel Mehandi ,&nbsp;Vishal Vijayvargiya","doi":"10.1016/j.kjs.2024.100303","DOIUrl":"10.1016/j.kjs.2024.100303","url":null,"abstract":"<div><p>The study explores the complex relationship between quantitative and qualitative characteristics in strawberries grown using a vertical farming system and artificial full spectrum light (AFSL). The research reveals the interdependencies between traits, identifies direct, and indirect effects on yield and quality, and highlights critical factors influencing overall variation in strawberry characteristics using correlation analysis, path-coefficient analysis, and principal component analysis (PCA). These discoveries open the door for specialized breeding and cultivation techniques that optimize vertical farming procedures and increase the sustainability and productivity of strawberries. This study confirmed the suitability of a four-layered vertical farming system for strawberry cultivation; however, an additional supply of AFSL at lower levels of verticals ensures a higher yield. The number of fruits per plant and average berry weight had a high (p_ij &gt; 0.3) degree of association with yield per plant at genotypic and phenotypic levels. The principal component analysis revealed a close association of T₁ (top level of vertical with natural light only), T₂ (third level from the top of vertical with natural light only), and T₅ (third level from the top of vertical with natural light and AFSL for 2 h) with yield contributing traits (number of bud formation, number of flowers, number of fruits per plant, fruit setting (%), fruit volume, average berry weight, average yield, and estimated yield per hectare).</p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100303"},"PeriodicalIF":1.2,"publicationDate":"2024-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001287/pdfft?md5=addeb1dcb5a026a2e973622752eca7f3&pid=1-s2.0-S2307410824001287-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141851131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of algD and oprL genes in Pseudomonas aeruginosa isolated from multiple environments and different local sources in Iraq","authors":"Afrah Abdullah Jassim ,&nbsp;Wael Mohammed Mahdi ,&nbsp;Omar Raheem Khalaf Al-Obaidi","doi":"10.1016/j.kjs.2024.100302","DOIUrl":"10.1016/j.kjs.2024.100302","url":null,"abstract":"<div><p>The aim of this study was to investigate the spread of the genes <em>alg</em>D and <em>opr</em>L in <em>P. aeruginosa</em> within the genetic structure of environmental sources of isolates and various clinical studies, and to evaluate a successful alternative for diagnosing <em>Pseudomonas aeruginosa (P. aeruginosa)</em> among various sources. In this study, 100 samples were collected from various clinical and environmental sources. Twenty-eight isolates of <em>P. aeruginosa</em> bacteria were isolated. Nine pathological samples were taken from wounds, burns, blood, and ear infections in three governorates: Salah al-Din, Baghdad, and Kirkuk. Five isolates of <em>P. aeruginosa</em> bacteria were collected from contaminated water from the Salah Al-Din and Baghdad governorates. The isolates were diagnosed phenotypically through the colony's shape, colour, odour and interaction with Gram stain. Additionally, the VITEK 2 system was used to confirm the diagnosis of the isolates. The results show that all the isolates belonged to the bacteria <em>P. aeruginosa.</em> The results of the molecular diagnosis using a polymerase chain reaction (PCR) technique for three genes with specialised primers (<em>16SrRNA</em>, <em>alg</em>D, and <em>opr</em>L) show that most of the bacterial isolates from different sources belong to the type <em>P. aeruginosa</em> because they contain the specific gene <em>16SrRNA</em> except for five isolates (M5, M7, M8, M9 and W1). Four of these belong to isolates with a pathogenic source and one was isolated from water. The results of the PCR for the <em>alg</em>D gene show that all isolates contain the gene except for four diseased samples. Samples M5, M7, M8, and M9 were without the gene, while all isolates had the <em>opr</em>L gene. A sequencing analysis of the DNA was then performed for the isolates that were not associated with the specialised primers of the above genes to ensure their genera (M5, M7, M8, and M9), in addition to a sample of 15 isolated from soil (S6) that was associated with all special primers for the same genes for the purpose of comparison. The results show that isolates M5, M7, M8 and M9 were diseased isolates belonging to <em>Escherichia coli</em>, while the 15 isolated from soil (S6) were diagnosed as <em>P. aeruginosa.</em></p></div>","PeriodicalId":17848,"journal":{"name":"Kuwait Journal of Science","volume":"52 1","pages":"Article 100302"},"PeriodicalIF":1.2,"publicationDate":"2024-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2307410824001275/pdfft?md5=ec33c910e00136da15bebaadd9914276&pid=1-s2.0-S2307410824001275-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141853302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"综合性期刊","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}