Journal of Zhejiang University SCIENCE B最新文献

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Improving RGB image recognition in the YOLO11n algorithm for accurate detection of tea plant diseases. 改进YOLO11n算法中的RGB图像识别,实现茶树病害的准确检测。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-05-15 DOI: 10.1631/jzus.B2500693
Jinxian Tao, Xiaoli Li, Jingfei Zhang, Muhammad Shoaib, Muhammad Adnan Islam, Ibrar Ahmad, Yong He, Sitan Ye, Yujie Wang, Binhui Liao, Mostafa Gouda
{"title":"Improving RGB image recognition in the YOLO11n algorithm for accurate detection of tea plant diseases.","authors":"Jinxian Tao, Xiaoli Li, Jingfei Zhang, Muhammad Shoaib, Muhammad Adnan Islam, Ibrar Ahmad, Yong He, Sitan Ye, Yujie Wang, Binhui Liao, Mostafa Gouda","doi":"10.1631/jzus.B2500693","DOIUrl":"10.1631/jzus.B2500693","url":null,"abstract":"<p><p>Tea diseases, including brown and gray blight, result in significant yield and quality losses, especially in Longjing tea production. Traditional detection methods are prone to errors, while existing deep learning models often struggle to be robust under natural field conditions. To address these challenges, an improved lightweight detection model, asymmetric multi-level (AML) mechanism, dynamic snake convolution (DSC), and scalable intersection over union (SIoU) loss function-You Only Look Once (YOLO) (ADS-YOLO), was developed and validated. In the method, a dataset comprising 5694 smartphone-captured images of tea leaves was established under natural lighting. Enhancements were implemented in the YOLO11n baseline algorithm through incorporation of the SIoU loss function for better bounding box regression, DSC, which realizes adaptive feature extraction based on the dynamic spatial context, and an AML mechanism, which achieves lightweight feature fusion via adaptive multi-scale design. The results showed that ADS-YOLO achieved a precision of 0.935 and a recall of 0.870, compared to 0.894 and 0.818, respectively, when the baseline YOLO11n was used. Importantly, ADS-YOLO demonstrated a real-time performance of 137.1 frames per second (FPS), coupled with reduced computational costs. ADS-YOLO improved the mean average precision (mAP) at intersection over union threshold of 0.5 (mAP@0.5) by 6.4% compared with YOLOv5n and achieved up to 44.6% higher accuracy than YOLOv7t. In conclusion, ADS-YOLO achieved high accuracy, providing a scalable solution for real-time crop health monitoring and sustainable precision agriculture for tea production.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 5","pages":"482-498"},"PeriodicalIF":5.7,"publicationDate":"2026-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13183483/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147973310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model. 施耐德膜源间充质干细胞在窦底抬高模型和颅骨缺损模型中的再生潜力。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-05-08 DOI: 10.1631/jzus.B2400611
Yuxin Zhao, Jia Wang, Dongqi You, Yifan Lu, Mengfei Yu, Misi Si
{"title":"Regenerative potential of Schneiderian membrane-derived mesenchymal stem cells in sinus floor elevation model and calvarial defect model.","authors":"Yuxin Zhao, Jia Wang, Dongqi You, Yifan Lu, Mengfei Yu, Misi Si","doi":"10.1631/jzus.B2400611","DOIUrl":"10.1631/jzus.B2400611","url":null,"abstract":"<p><p><b>OBJECTIVES</b>: Schneiderian membrane-derived mesenchymal stem cells (SMMSCs) have been reported to be osteogenic progenitor cells in vitro. However, there is controversy regarding the intrinsic osteogenic capacity of the Schneiderian membrane, and the bone formation potential of SMMSCs in vivo has never been reported. Therefore, in this study, we aimed to evaluate the contribution of the Schneiderian membrane to sinus floor elevation and to verify the function of SMMSCs in cranial bone defects. <b>MATERIALS AND METHODS</b>: Bilateral sinus floor elevation with chloromethyl-benzamidodialkylcarbocyanine (CM-Dil) labeling was performed in rabbits to assess Schneiderian membrane osteogenesis. Single-cell RNA sequencing was used to characterize human Schneiderian membrane cellular subsets. SMMSCs and bone marrow-derived mesenchymal stem cells (BMSCs) were transplanted into rabbit cranial defects with gelatin methacryloyl (GelMA) scaffolds and analyzed via micro-computed tomography (micro-CT) and histology. <b>RESULTS</b>: Spontaneous bone formation adjacent to the Schneiderian membrane was observed. Single-cell analysis identified paired-related homeobox 1 (<i>PRRX1</i>) progenitor clusters driving endosinus osteogenesis. SMMSCs exhibited earlier and superior bone regeneration compared with BMSCs, with higher tissue volume and bone volume/total volume (BV/TV) ratios at four weeks after surgery. <b>CONCLUSIONS</b>: The Schneiderian membrane likely contributes to osteogenesis via <i>PRRX1</i><sup>+</sup> progenitor lineages. SMMSCs promote accelerated early bone regeneration in cranial defects. This study provides the first in vivo validation of the osteogenic capacity of SMMSCs and defines their molecular identity at single-cell resolution.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 6","pages":"628-644"},"PeriodicalIF":5.7,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294289/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
HENMT1: an RNA methyltransferase in biology and disease. HENMT1:生物学和疾病中的RNA甲基转移酶。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-05-04 DOI: 10.1631/jzus.B2500059
Shanghong Jiang, Yongchao Zhao, Danrui Cui
{"title":"HENMT1: an RNA methyltransferase in biology and disease.","authors":"Shanghong Jiang, Yongchao Zhao, Danrui Cui","doi":"10.1631/jzus.B2500059","DOIUrl":"10.1631/jzus.B2500059","url":null,"abstract":"<p><p>HEN methyltransferase 1 (HENMT1) is an RNA methyltransferase that catalyzes 2'-<i>O</i>-methylation of P-element-induced wimpy testis (PIWI)-interacting RNAs (piRNAs) within the small RNA silencing pathway and methylates microRNAs (miRNAs). By stabilizing these RNA molecules, HENMT1 plays a pivotal role in regulating the biological processes that they target, thereby maintaining cellular homeostasis. Mutations in <i>HENMT1</i> homologs across various species have been shown to alter biological traits and impair reproduction. <i>HENMT1</i> mutations have been linked to infertility and tumor development in humans. This comprehensive review first introduces the structure and function of HENMT1 and its homologs, with a focus on elucidating the piRNA methylation process. Next, we examine the aberrant expression of HENMT1 in human cancers and its relationship with immune infiltration by analyzing the Cancer Genome Atlas (TCGA) database and tumor immune infiltration profiling, providing insights into the dysregulation of HENMT1 in diseases such as infertility and cancer. Finally, we discuss current knowledge and future perspectives on HENMT1's function in cancer progression.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 6","pages":"590-602"},"PeriodicalIF":5.7,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294282/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Esculetin attenuates migraine-like pain via CGRP suppression and meningeal mast cell modulation in rat models. 在大鼠模型中,艾斯维素通过抑制CGRP和调节脑膜肥大细胞减轻偏头痛样疼痛。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-05-01 DOI: 10.1631/jzus.B2500147
Ayca Nur Gonul, Ibrahim Ethem Torun, Yasemin Baranoglu Kilinc, Erkan Kilinc
{"title":"Esculetin attenuates migraine-like pain via CGRP suppression and meningeal mast cell modulation in rat models.","authors":"Ayca Nur Gonul, Ibrahim Ethem Torun, Yasemin Baranoglu Kilinc, Erkan Kilinc","doi":"10.1631/jzus.B2500147","DOIUrl":"10.1631/jzus.B2500147","url":null,"abstract":"<p><p>Growing evidence suggests that esculetin, a 5-lipoxygenase inhibitor, has pharmacotherapeutic potential due to its various pharmacological properties, such as potent anti-inflammatory, anti-nociceptive, and γ-aminobutyric acid type A (GABA<sub>A</sub>) receptor partial agonist activities. However, the effects of this promising agent on migraine remain unexplored. This study therefore examined the impact of esculetin on relevant mechanisms in migraine-like conditions in rats. The systemic effects of esculetin at three distinct doses (5, 10, and 20 mg/kg) were tested in a nitroglycerin (NTG)-induced migraine model using in vivo experimental sets. The direct action of esculetin on the release of calcitonin gene-related peptide (CGRP) from critical structures of the trigeminovascular system (trigeminal ganglion, trigeminal nucleus, and meningeal afferents) was also tested in ex vivo experimental sets. Sumatriptan was used as a positive control in both sets of experiments. The in vivo results showed that esculetin reduced NTG-induced mechanical hyperalgesia and decreased trigeminal CGRP and cellular Fos proto-oncogene (c-Fos) levels. It also decreased degranulation and meningeal mast cell numbers. The ex vivo results revealed that esculetin reduced NTG-stimulated CGRP release from trigeminovascular explants, with the exception of meningeal explants. Sumatriptan reversed the NTG-induced changes in both experimental sets. Our findings suggest that esculetin exhibits anti-nociceptive activities in experimental migraine conditions, alleviating trigeminovascular CGRP concentrations and the degranulation of meningeal mast cells. Esculetin may thus represent a therapeutic option for relieving migraine headaches, although further research is needed to confirm this.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 6","pages":"615-627"},"PeriodicalIF":5.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294283/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of the immune microenvironment in bone regeneration and progress in immune-modulating bone nanobiomaterials. 免疫微环境在骨再生中的作用及免疫调节骨纳米生物材料的研究进展。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-28 DOI: 10.1631/jzus.B2400472
Rong'an Li, Jiakang Yang, Wenjun Duan, Kexin Yang, Katong Lo, Qianming Chen, Baixiang Wang
{"title":"Role of the immune microenvironment in bone regeneration and progress in immune-modulating bone nanobiomaterials.","authors":"Rong'an Li, Jiakang Yang, Wenjun Duan, Kexin Yang, Katong Lo, Qianming Chen, Baixiang Wang","doi":"10.1631/jzus.B2400472","DOIUrl":"https://doi.org/10.1631/jzus.B2400472","url":null,"abstract":"<p><p>Inflammation is a key physiological process in the regeneration of bone tissue following injury. The acute inflammatory response, along with the timely resolution of inflammation, is essential for effective bone tissue repair. Exacerbation of either acute or chronic inflammation can lead to impaired bone regeneration, which is closely associated with interactions between immune cells and bone-related cells, as well as the regulatory roles of various inflammatory cytokines. In this review, we discuss the role of the immune microenvironment in bone regeneration and the negative impact of dysregulated inflammation on bone regeneration, and highlight on the need for timely elimination of inflammation. Additionally, the application of nanobiomaterials with immunomodulatory function in the treatment of inflammatory bone defects is discussed to clarify its current challenges and the future direction of its development.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 8","pages":"817-831"},"PeriodicalIF":5.7,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13474198/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Halotolerant plant growth-promoting bacteria Enterobacter sp. Av16 and Acinetobacter sp. Av23 enhance seed germination and seedling photosynthesis of Apocynum pictum under salt stress. 耐盐植物促生长细菌肠杆菌sp. Av16和不动杆菌sp. Av23在盐胁迫下促进罗布麻种子萌发和幼苗光合作用。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-27 DOI: 10.1631/jzus.B2500049
Xue Wang, Li Jiang, Yao Ge, Yiping Zou, Qingsheng Cai, Yan Xia, Laiqing Lou
{"title":"Halotolerant plant growth-promoting bacteria <i>Enterobacter</i> sp. Av16 and <i>Acinetobacter</i> sp. Av23 enhance seed germination and seedling photosynthesis of <i>Apocynum pictum</i> under salt stress.","authors":"Xue Wang, Li Jiang, Yao Ge, Yiping Zou, Qingsheng Cai, Yan Xia, Laiqing Lou","doi":"10.1631/jzus.B2500049","DOIUrl":"10.1631/jzus.B2500049","url":null,"abstract":"<p><p><i>Apocynum pictum</i> Schrenk, a halophyte, is commonly used as a traditional Chinese medicine, tea, and fiber crop. To improve the growth of <i>A</i>. <i>pictum</i> in saline soil, its responses to halotolerant plant growth-promoting bacteria (PGPB) were investigated at germination and during early growth stages. Inoculation with either <i>Enterobacter</i> sp. Av16 or <i>Acinetobacter</i> sp. Av23 significantly improved seed germination percentage and alleviated the adverse effects of salinity on seedling growth of <i>A</i>. <i>pictum</i>. Under salt stress, PGPB increased leaf area and improved photosynthetic pigments, including chlorophyll <i>a</i>+<i>b</i> and carotenoids, as well as intercellular carbon dioxide (<i>C</i><sub>i</sub>) and transpiration rate (<i>T</i><sub>r</sub>). More importantly, PGPB alleviated salt-induced damage to the photosynthetic apparatus by stabilizing the photosystems and optimizing electron transport processes. This was evidenced by increases in the density of reaction centers per cross-section (RC/CS<sub>m</sub>) and the efficiencies of electron transfer to photosystem I (δ<i>R</i><sub>o</sub> and Φ<i>R</i><sub>o</sub>). Consequently, PGPB improved chlorophyll fluorescence and key photosynthetic parameters, including the maximum quantum yield (Φ<i>P</i><sub>o</sub>), performance index on absorption basis (PI<sub>abs</sub>), overall performance index (PI<sub>total</sub>), and net photosynthetic rate (<i>P</i><sub>n</sub>). Furthermore, PGPB activated antioxidant enzymes, such as superoxide dismutase (SOD) and catalase (CAT), reducing the accumulation of reactive oxygen species (ROS) in <i>A</i>. <i>pictum</i>. In summary, PGPB enhanced <i>A</i>. <i>pictum</i> seed germination and photosynthetic capacity by stabilizing photosystems, improving stomatal gas exchange, and mitigating oxidative stress under salt stress. These findings highlight the potential of PGPB inoculation as a sustainable strategy to enhance salt resilience in <i>A</i>. <i>pictum</i>.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 6","pages":"656-670"},"PeriodicalIF":5.7,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13294292/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148317954","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TAF1 aggravates ferroptosis by promoting the ubiquitin-mediated degradation of nuclear GPX4. TAF1通过促进泛素介导的核GPX4降解而加重铁下垂。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-23 DOI: 10.1631/jzus.B2500567
Kehong Ye, Meifu Gan, Liang Sun, Chaoyi Chen, Xuan Lai, Yinjun He, Ming Zhu, Weiqin Jiang, Honghe Zhang
{"title":"TAF1 aggravates ferroptosis by promoting the ubiquitin-mediated degradation of nuclear GPX4.","authors":"Kehong Ye, Meifu Gan, Liang Sun, Chaoyi Chen, Xuan Lai, Yinjun He, Ming Zhu, Weiqin Jiang, Honghe Zhang","doi":"10.1631/jzus.B2500567","DOIUrl":"10.1631/jzus.B2500567","url":null,"abstract":"<p><p>Glutathione peroxidase 4 (GPX4) is a primary inhibitor of ferroptosis, a regulated form of cell death driven by the accumulation of lipid hydroperoxides. GPX4 exists in three isoforms localized in the cytosol, mitochondria, and nucleus; however, the regulatory mechanisms governing nuclear GPX4 (nGPX4) remain largely unclear. Herein, we identified TATA box-binding protein-associated factor 1 (TAF1) as a pivotal regulator of nGPX4. TAF1 phosphorylates nGPX4, leading to its lysine 11 (K11)-linked ubiquitination and proteasomal degradation, thereby promoting ferroptosis in tumor protein p53 (<i>TP53</i>)-mutant cells. Conversely, in <i>TP53</i>-wild-type (WT) cells, TAF1 phosphorylates TP53, facilitating murine double minute 2 (MDM2)-mediated TP53 degradation, which upregulates solute carrier family 7 member 11 (<i>SLC7A11</i>) expression and reduces cellular susceptibility to ferroptosis. Collectively, TAF1 plays dual and context-dependent roles in ferroptosis regulation, acting as both a promoter and an inhibitor depending on the <i>TP53</i> status.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 4","pages":"359-374"},"PeriodicalIF":5.7,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121887/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Infra-slow body-surface potentials show a group-level association with thyroid cancer. 次慢体表电位显示与甲状腺癌有群体水平的关联。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-23 DOI: 10.1631/jzus.B2600040
Bohan Deng, Ruili Zhang, Yuxuan Liu, Jinglao Lin, Shicong Gui, Xihao Wei, Yin Cheng, Li Zheng, Shaohua Hu, Pingping Lyu, Yubo Li, Huafen Wang
{"title":"Infra-slow body-surface potentials show a group-level association with thyroid cancer.","authors":"Bohan Deng, Ruili Zhang, Yuxuan Liu, Jinglao Lin, Shicong Gui, Xihao Wei, Yin Cheng, Li Zheng, Shaohua Hu, Pingping Lyu, Yubo Li, Huafen Wang","doi":"10.1631/jzus.B2600040","DOIUrl":"10.1631/jzus.B2600040","url":null,"abstract":"<p><p>Thyroid cancer is a common malignancy with broad systemic endocrine and metabolic effects (Hammond et al., 2024). Regarding diagnostics, if infra-slow body-surface potential (BSP) features reflect a systemic state, then this type of cancer is a reasonable clinical test case. Prior electrodermal work has focused on faster transients above 0.5 Hz (Boucsein, 2012; Posada-Quintero and Chon, 2020), and task-state skin potential abnormalities have been shown to distinguish mood disorders from healthy controls (Lyu et al., 2024). We concentrated on an infra-slow band from 0.025 to 0.2 Hz, called Band 1 herein. We evaluated whether infra-slow features carry reproducible group-level information when artifacts are explicitly controlled, with one pre-specified primary endpoint. \"Separable\" here means a group-level statistical difference, not individual-level diagnosis. In a cohort of 321 cancer patients, Band 1 energy showed a clear group-level association with thyroid cancer, which persisted after automated quality control (QC) and under stricter artifact-removal settings.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 4","pages":"426-430"},"PeriodicalIF":5.7,"publicationDate":"2026-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121882/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route. 宏基因组测序显示,通过下肠途径将人类供体微生物群移植到无菌小鼠体内具有很高的可重复性。
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-22 DOI: 10.1631/jzus.B2400495
Yapeng Yang, Xiang Tan, Zeyue Zhang, Lifeng Liang, Zhifeng Wu, Jinhui He, Yuqing Wang, Miaomiao Dong, Jixia Zheng, Hang Zhang, Shuaifei Feng, Wei Cheng, Bota Cui, Hong Wei, Qinjin Li
{"title":"Metagenomic sequencing reveals high reproducibility of human donor microbiota transplanted into germ-free mice via lower gut route.","authors":"Yapeng Yang, Xiang Tan, Zeyue Zhang, Lifeng Liang, Zhifeng Wu, Jinhui He, Yuqing Wang, Miaomiao Dong, Jixia Zheng, Hang Zhang, Shuaifei Feng, Wei Cheng, Bota Cui, Hong Wei, Qinjin Li","doi":"10.1631/jzus.B2400495","DOIUrl":"10.1631/jzus.B2400495","url":null,"abstract":"<p><p>Human flora-associated (HFA) mice are often used to simulate the structure of human intestinal microbiota and to study the causal relationships between diseases and gut microbiota. However, several factors affect the colonization efficiency of human microbiota in germ-free (GF) mice, and the differential effects of gavage and lower gut transplantation on colonization are still unclear. In this study, we explored the reproducibility of the recipient-to-donor gut microbiota community structure and function under different transplantation routes and the differences in microbial colonization between recipients via gavage transplantation (GT_mice group) and lower gut transplantation (LGT_mice group). High-throughput sequencing of the metagenome was performed on the feces of each subject, and the composition of microbiome of each group was analyzed. As expected, the introduction of human fecal microbiota into GF mice via lower gut transplantation had a high transfer efficiency, which was evident from the similar species community structure to that of the donor (Adonis <i>R</i><sup>2</sup>=0.713 960 for LGT_mice group‒donor group; Adonis <i>R</i><sup>2</sup>=0.774 095 for GT_mice group‒donor group) and a higher bacterial colonization rate. The findings provide unique insights into improving the accuracy of constructing humanized microbiota transplantation models, aiding our understanding of the relationships between the human gut microbiota and disease.</p>","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"27 4","pages":"375-389"},"PeriodicalIF":5.7,"publicationDate":"2026-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13121883/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147775492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Deep learning-based phenology extraction and crop classification in arid oasis using Sentinel-2 time series. 基于Sentinel-2时间序列的干旱绿洲物候提取与作物分类
IF 5.7 3区 生物学
Journal of Zhejiang University SCIENCE B Pub Date : 2026-04-15 DOI: 10.1631/jzus.B2500403
Chunli Wang, Jianan Chi, Xiao Zhang, Nannan Zhang
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