Journal of Zhejiang University SCIENCE B最新文献

Single-cell transcriptome analysis reveals abnormal angiogenesis and placentation by loss of imprinted glutaminyl-peptide cyclotransferase. 单细胞转录组分析显示，由于谷氨酰胺肽环转移酶印迹的缺失，血管生成和胎盘异常。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-06-15 DOI: 10.1631/jzus.B2400099

Jing Guo, Jihong Zheng, Ruixia Li, Jindong Yao, He Zhang, Xu Wang, Chao Zhang

{"title":"Single-cell transcriptome analysis reveals abnormal angiogenesis and placentation by loss of imprinted glutaminyl-peptide cyclotransferase.","authors":"Jing Guo, Jihong Zheng, Ruixia Li, Jindong Yao, He Zhang, Xu Wang, Chao Zhang","doi":"10.1631/jzus.B2400099","DOIUrl":"10.1631/jzus.B2400099","url":null,"abstract":"Imprinted genes play a key role in regulating mammalian placental and embryonic development. Here, we generated glutaminyl-peptide cyclotransferase-knockout (Qpct-/-) mice utilizing the clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) platform and identified Qpct as a novel anti-angiogenic factor in regulating mouse placentation. Compared with Qpct+/+ mice, placentae and embryos (Qpct-/+ and Qpct-/-) showed significant overgrowth at embryonic Day 12.5 (E12.5), E15.5, and E18.5. Using single-cell transcriptome analysis of 32 309 cells from Qpct+/+ and Qpct-/- mouse placentae, we identified 13 cell clusters via single-nucleus RNA sequencing (snRNA-seq) (8880 Qpct+/+ and 13 577 Qpct-/- cells) and 20 cell clusters via single-cell RNA sequencing (scRNA-seq) (6567 Qpct+/+ and 3285 Qpct-/- cells). Furthermore, we observed a global up-regulation of pro-angiogenic genes in the Qpct-/- background. Immunohistochemistry assays revealed a notable increase in the number of blood vessels in the decidual and labyrinthine layers of E15.5 Qpct-/+ and Qpct-/- mice. Moreover, the elevation of multiple pairs of ligand-receptor interactions was observed in decidual cells, endothelial cells, and macrophages, promoting angiogenesis and inflammatory response. Our findings indicate that loss of maternal Qpct leads to altered phenotypic characteristics of placentae and embryos and promotes angiogenesis in murine placentae.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 6","pages":"589-608"},"PeriodicalIF":4.7,"publicationDate":"2025-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Seeing the macro in the micro: a diffusion model-based approach for style transfer in cellular images. 从微观看宏观：基于扩散模型的细胞图像风格转移方法。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-06-11 DOI: 10.1631/jzus.B2500012

Jiayi Cai, Yong He, Feng Liu, Byung-Ho Kang, Xuping Feng

{"title":"Seeing the macro in the micro: a diffusion model-based approach for style transfer in cellular images.","authors":"Jiayi Cai, Yong He, Feng Liu, Byung-Ho Kang, Xuping Feng","doi":"10.1631/jzus.B2500012","DOIUrl":"10.1631/jzus.B2500012","url":null,"abstract":"The internal structures of cells as the basic units of life are a major wonder of the microscopic world. Cellular images provide an intriguing window to help explore and understand the composition and function of these structures. Scientific imagery combined with artistic expression can further expand the potential of imaging in educational dissemination and interdisciplinary applications. This study presents an innovative diffusion model-based approach for style transfer in cellular images, combining scientific rigor with artistic expression. By leveraging training-free large-scale pre-trained diffusion models, the proposed method integrates the intricate morphological and textural features of cellular images with diverse artistic styles. Key techniques such as the inversion of denoising diffusion implicit models (DDIMs), adaptive instance normalization (AdaIN), self-attention style injection, and attention temperature scaling ensure the preservation of cellular structures while enhancing visual expressiveness. The results showcase the potential of this strategy for interdisciplinary applications, enriching both the visualization and educational dissemination of cellular imagery through compelling storytelling and aesthetic appeal.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 6","pages":"609-612"},"PeriodicalIF":4.7,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219496/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Applications of bioactive peptides in cosmeceuticals: a review. 生物活性肽在药妆中的应用综述。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-06-11 DOI: 10.1631/jzus.B2400029

Tolulope Joshua Ashaolu

引用次数: 0

Fibroblast growth factors and endometrial decidualization: models, mechanisms, and related pathologies. 成纤维细胞生长因子与子宫内膜去个体化：模型、机制和相关病理。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-06-02 DOI: 10.1631/jzus.B2300830

Xueni Zhang, Yidi Mo, Chunbin Lu, Zhijian Su, Xiaokun Li

{"title":"Fibroblast growth factors and endometrial decidualization: models, mechanisms, and related pathologies.","authors":"Xueni Zhang, Yidi Mo, Chunbin Lu, Zhijian Su, Xiaokun Li","doi":"10.1631/jzus.B2300830","DOIUrl":"10.1631/jzus.B2300830","url":null,"abstract":"The onset of pregnancy is marked by the formation of a zygote, while the culmination of gestation is manifested by the delivery of a fetus. Meanwhile, a successful pregnancy entails a meticulously coordinated sequence of events from embryo implantation to sustained decidualization of the uterus to placental development and childbirth. The decidual reaction, a pivotal process occurring within the endometrium during pregnancy, is finely regulated by sex steroids and cytokines. Notably, fibroblast growth factors (FGFs), particularly FGF2, play a critical role in this physiological cascade. Dysregulated FGF expression may trigger inadequate decidualization, precipitating a spectrum of adverse pregnancy outcomes, including preeclampsia, recurrent implantation failure, and miscarriage. Furthermore, the human decidua, distinct from most mammalian species and similar to great apes, undergoes regular cycles of formation and shedding, independent of the presence of the embryo in the endometrium. This process is also tightly controlled by various FGFs. In this review, we comprehensively compare diverse research decidualization models, delineate the trend of endometrial FGFs during the menstrual cycle, and provide a synopsis of endometrial diseases triggered by FGF dysregulation.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 6","pages":"573-588"},"PeriodicalIF":4.7,"publicationDate":"2025-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219500/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mechanisms of ribosomopathy and phase separation-related ribosomopathy. 核糖体病和相分离相关核糖体病的机制。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-06-02 DOI: 10.1631/jzus.B2300904

Zhiyuan Pan, Guofen Lin, Hao Liu, Guozhi Li, Xiaoyi Zhang, Jiewen Dai

引用次数: 0

Metagenomics reveals an increased proportion of an Escherichia coli-dominated enterotype in elderly Chinese people. 宏基因组学显示，中国老年人大肠杆菌为主的肠道型比例增加。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-05-28 DOI: 10.1631/jzus.B2400341

Jinyou Li, Yue Wu, Yichen Yang, Lufang Chen, Caihong He, Shixian Zhou, Shunmei Huang, Xia Zhang, Yuming Wang, Qifeng Gui, Haifeng Lu, Qin Zhang, Yunmei Yang

{"title":"Metagenomics reveals an increased proportion of an Escherichia coli-dominated enterotype in elderly Chinese people.","authors":"Jinyou Li, Yue Wu, Yichen Yang, Lufang Chen, Caihong He, Shixian Zhou, Shunmei Huang, Xia Zhang, Yuming Wang, Qifeng Gui, Haifeng Lu, Qin Zhang, Yunmei Yang","doi":"10.1631/jzus.B2400341","DOIUrl":"10.1631/jzus.B2400341","url":null,"abstract":"Gut microbial communities are likely remodeled in tandem with accumulated physiological decline during aging, yet there is limited understanding of gut microbiome variation in advanced age. Here, we performed a metagenomics-based enterotype analysis in a geographically homogeneous cohort of 367 enrolled Chinese individuals between the ages of 60 and 94 years, with the goal of characterizing the gut microbiome of elderly individuals and identifying factors linked to enterotype variations. In addition to two adult-like enterotypes dominated by Bacteroides (ET-Bacteroides) and Prevotella (ET-Prevotella), we identified a novel enterotype dominated by Escherichia (ET-Escherichia), whose prevalence increased in advanced age. Our data demonstrated that age explained more of the variance in the gut microbiome than previously identified factors such as type 2 diabetes mellitus (T2DM) or diet. We characterized the distinct taxonomic and functional profiles of ET-Escherichia, and found the strongest cohesion and highest robustness of the microbial co-occurrence network in this enterotype, as well as the lowest species diversity. In addition, we carried out a series of correlation analyses and co-abundance network analyses, which showed that several factors were likely linked to the overabundance of Escherichia members, including advanced age, vegetable intake, and fruit intake. Overall, our data revealed an enterotype variation characterized by Escherichia enrichment in the elderly population. Considering the different age distribution of each enterotype, these findings provide new insights into the changes that occur in the gut microbiome with age and highlight the importance of microbiome-based stratification of elderly individuals.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 5","pages":"477-492"},"PeriodicalIF":4.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in antibody optimization based on deep learning methods. 基于深度学习方法的抗体优化研究进展。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-05-28 DOI: 10.1631/jzus.B2400387

Ruofan Jin, Ruhong Zhou, Dong Zhang

引用次数: 0

Unmet needs of patients with intravascular large B-cell lymphoma: three case reports and a literature review. 血管内大b细胞淋巴瘤患者未满足的需求：三例报告和一篇文献综述。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-05-28 DOI: 10.1631/jzus.B2300941

Xian Li, Ru Luo, Jiaming Xu, Xueli Jin, Weiqin Wang, Xibin Xiao, Wenbin Qian

引用次数: 0

Pharmacological inhibition of ENaC or NCX can attenuate hepatic ischemia-reperfusion injury exacerbated by hypernatremia. 药物抑制ENaC或NCX可减轻高钠血症加重的肝缺血再灌注损伤。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-05-28 DOI: 10.1631/jzus.B2300825

Yabin Chen, Hao Li, Peihao Wen, Jiakai Zhang, Zhihui Wang, Shengli Cao, Wenzhi Guo

{"title":"Pharmacological inhibition of ENaC or NCX can attenuate hepatic ischemia-reperfusion injury exacerbated by hypernatremia.","authors":"Yabin Chen, Hao Li, Peihao Wen, Jiakai Zhang, Zhihui Wang, Shengli Cao, Wenzhi Guo","doi":"10.1631/jzus.B2300825","DOIUrl":"10.1631/jzus.B2300825","url":null,"abstract":"Donors with a serum sodium concentration of >155 mmol/L are extended criteria donors for liver transplantation (LT). Elevated serum sodium of donors leads to an increased incidence of hepatic dysfunction in the early postoperative period of LT; however, the exact mechanism has not been reported. We constructed a Lewis rat model of 70% hepatic parenchymal area subjected to ischemia-reperfusion (I/R) with hypernatremia and a BRL-3A cell model of hypoxia-reoxygenation (H/R) with high-sodium (HS) culture medium precondition. To determine the degree of injury, biochemical analysis, histological analysis, and oxidative stress and apoptosis detection were performed. We applied specific inhibitors of the epithelial sodium channel (ENaC) and Na+/Ca2+ exchanger (NCX) in vivo and in vitro to verify their roles in injury. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and lactate dehydrogenase (LDH) levels and the area of hepatic necrosis were significantly elevated in the HS+I/R group. Increased reactive oxygen species (ROS) production, myeloperoxidase (MPO)‍-positive cells, and aggravated cellular apoptosis were detected in the HS+I/R group. The HS+H/R group of BRL-3A cells showed significantly increased cellular apoptosis and ROS production compared to the H/R group. The application of amiloride (Amil), a specific inhibitor of ENaC, reduced ischemia-reperfusion injury (IRI) aggravated by HS both in vivo and in vitro, as evidenced by decreased serum transaminases, inflammatory cytokines, apoptosis, and oxidative stress. SN-6, a specific inhibitor of NCX, had a similar effect to Amil. In summary, hypernatremia aggravates hepatic IRI, which can be attenuated by pharmacological inhibition of ENaC or NCX.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 5","pages":"461-476"},"PeriodicalIF":4.7,"publicationDate":"2025-05-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12119183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144174148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential role of FNDC5 in exercise-induced improvement of cognitive function. FNDC5在运动诱导的认知功能改善中的潜在作用。

IF 4.7 3区生物学

Journal of Zhejiang University SCIENCE B Pub Date : 2025-05-23 DOI: 10.1631/jzus.B2400016

Ruobing Zhao, Xuchang Zhou, Dongxue Wang, Haifeng Tang, Guoxin Ni

{"title":"Potential role of FNDC5 in exercise-induced improvement of cognitive function.","authors":"Ruobing Zhao, Xuchang Zhou, Dongxue Wang, Haifeng Tang, Guoxin Ni","doi":"10.1631/jzus.B2400016","DOIUrl":"10.1631/jzus.B2400016","url":null,"abstract":"Cognitive dysfunction often occurs in Alzheimer's disease, Parkinson's disease, cerebrovascular disease, or other neurodegenerative diseases, and can significantly impact the life quality of patients and create serious social, psychological, and economic burdens for individuals and their families. Numerous studies have confirmed that exercise can slow the decline in cognitive function through multiple pathways, in which fibronectin type III domain-containing protein 5 (FNDC5) plays an important role. However, the current research on the modulation of FNDC5 by exercise and its ability to improve hippocampal cognitive function lacks a systematic and comprehensive understanding. Therefore, this review focuses on the latest research progress regarding the role of exercise-induced FNDC5 in cognitive function, systematically reviews the positive effects of FNDC5 on cognitive function impairment caused by various factors, and clarifies the specific mechanisms by which exercise-induced FNDC5 improves cognitive function by inhibiting neuroinflammation and improving hippocampal neurogenesis and hippocampal synaptic plasticity. Based on the existing literature, we also identify the areas that require further research in this field. Overall, this review provides a theoretical basis for exercise-based prevention and improvement of cognitive function impairment.","PeriodicalId":17797,"journal":{"name":"Journal of Zhejiang University SCIENCE B","volume":"26 6","pages":"557-572"},"PeriodicalIF":4.7,"publicationDate":"2025-05-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12219485/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144506064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0