Palma-Jacinto José Antonio, Santiago-Roque Isela, A. Elind
{"title":"Hypercaloric Cafeteria Diet-Induces Obesity, Dyslipidemia, Insulin Resistance, Inflammation and Oxidative Stress in Wistar Rats","authors":"Palma-Jacinto José Antonio, Santiago-Roque Isela, A. Elind","doi":"10.21776/ub.jels.2023.013.01.03","DOIUrl":"https://doi.org/10.21776/ub.jels.2023.013.01.03","url":null,"abstract":"","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126511562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Radita Intan Aisyah Pratiwi, S. Widyarti, S. Sumitro
{"title":"Potential of Kesambi Active Compound (Schleichera oleosa) as Antagonist G-Protein Estrogen Receptor 1 (GPER1) by In Silico","authors":"Radita Intan Aisyah Pratiwi, S. Widyarti, S. Sumitro","doi":"10.21776/ub.jels.2023.013.01.07","DOIUrl":"https://doi.org/10.21776/ub.jels.2023.013.01.07","url":null,"abstract":"","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126352335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Relationship between Metallothionein and Mercury (Hg) in the Gill Tissue of the Barbonymus altus in the Brantas River Jombang, East Java","authors":"Aang Setyawan Anjasmara, A. Hertika, U. Yanuhar","doi":"10.21776/ub.jels.2023.013.01.09","DOIUrl":"https://doi.org/10.21776/ub.jels.2023.013.01.09","url":null,"abstract":"","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122523846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Relationship of Chemical Compounds and Crystal Development in Porang Tuber (Amorphophallus muelleri Blume)","authors":"Nurul Chairiyah, N. Harijati, R. Mastuti","doi":"10.21776/ub.jels.2023.013.01.01","DOIUrl":"https://doi.org/10.21776/ub.jels.2023.013.01.01","url":null,"abstract":"","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130478183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ramanda Ahmad Rizal Rifa'i, S. Andayani, M. Fadjar
{"title":"Antibacterial Ability of Rhizophora mucronata Leaf Extract Against Bacterial Infections of Edwardsiella tarda","authors":"Ramanda Ahmad Rizal Rifa'i, S. Andayani, M. Fadjar","doi":"10.21776/ub.jels.2023.013.01.02","DOIUrl":"https://doi.org/10.21776/ub.jels.2023.013.01.02","url":null,"abstract":"","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129913229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Isolation and Screening of Lactic Acid Bacteria From Sumbawa Buffalo Milk (Bubalus bubalis) as Potential Starter Cultures","authors":"Deni Harmoko, T. Ardyati, Y. Jatmiko","doi":"10.21776/ub.jels.2022.012.03.03","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.03","url":null,"abstract":"The Sumbawa buffalo (Bubalus bubalis) is one of the ruminant livestock in Indonesia that not only contributes to fulfilling meat requirements but also milk. Besides containing nutrients that are very beneficial for human health, buffalo milk is also a potential source of lactic acid bacteria (LAB) with technological and functional properties. Lactic acid bacteria have been utilized as starter cultures in various fermented products. This study aimed to isolate LAB from Sumbawa buffalo milk and to identify the potential isolate as a starter culture. The screening of LAB as a starter culture was based on some technological properties, including proteolytic activity, lipolytic activity, exopolysaccharide (EPS) production, antibacterial activity, antibiotic sensitivity, hemolytic activity, and acidification activity Data were analyzed statistically using one-way ANOVA and Tukey’s post hoc test at a 5% significance level. A total of 21 isolates were isolated from fresh buffalo milk, with a LAB total was 4.7x105 CFU.mL-1. All the isolates were characterized as Gram-positive with cocci-shaped. The SA8 isolate was selected as the most potential candidate as a starter culture because it has fulfilled the criteria such as the highest proteolytic activity, the lowest lipolytic activity, producing EPS, potential antagonistic activity against Bacillus cereus, Escherichia coli, and Salmonella Typhi, and sensitivity to cefazolin, intermediate to erythromycin and cinoxacin, non-pathogen, as well as the most rapid acidification activity. The SA8 isolate was identified as Enterococcus lactis with a similarity level of 99.99% towards strain BT159. This indigenous LAB was a potential starter culture of Sumbawa fermented buffalo milk to increase the diversification of products derived from buffalo milk.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116341693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Galang Ayuz Firstian Adjid Adjid, A. Kurniawan, N. Nazriati
{"title":"Textile Industry Waste Pollution in the Konto River: A Comparison of Public Perceptions and Water Quality Data","authors":"Galang Ayuz Firstian Adjid Adjid, A. Kurniawan, N. Nazriati","doi":"10.21776/ub.jels.2022.012.03.05","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.05","url":null,"abstract":"Textile industry production activities in one of the Badas areas can have implications for the occurrence of dye liquid waste pollution in the Konto river flow. This implication leads to the public perception that there has been river pollution from textile dyeing liquid waste. In this case, public perceptions need to be analyzed as a form of river environmental monitoring activities. Therefore, public perceptions of pollution were compared with actual water quality data, especially related to dyes in river water, to link public perceptions with environmental resource management efforts. This study aims to analyze public perceptions of textile dye waste pollution and compare the analysis results with the river water quality analysis. Analysis of river water quality (dye content, BOD, COD) was conducted at five sampling points in Badas, Kuwik, Balungjeruk, and Wonorejo villages. Public perception was measured descriptively through interviews with respondents referring to the Slovin method. The analysis of public perceptions shows that the Public considers that there has been pollution of river water, mainly due to textile industry waste, along the Konto River. The results of the water quality analysis showed that dye concentrations were found at four sampling points except for Wonorejo Village. This result shows that the correlation between public perception and data on dye contamination only occurs in Badas, Kuwik, and Balungjeruk villages. In addition, the BOD/COD ratio indicates that pollution has occurred at all observation points in this study.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128334522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nida Asfi, Y. Christina, D. Dwijayanti, Muhaimin Rifa’i, M. S. Djati
{"title":"Anti-inflammatory Activity of Elephantopus scaber and Sauropus androgynus Combination in Pregnant Mice Infected with Escherichia coli","authors":"Nida Asfi, Y. Christina, D. Dwijayanti, Muhaimin Rifa’i, M. S. Djati","doi":"10.21776/ub.jels.2022.012.03.06","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.06","url":null,"abstract":"The current study aimed to investigate the effectiveness of Elephantopus scaber and Sauropus androgynus as anti-inflammatory agents in pregnant mice infected with Escherichia coli. This study used seven treatments group (n=3): K- (healthy pregnant mice), K+ (pregnant mice infected with E. coli), P1 (pregnant mice infected with E. coli + E. scaber 100%), P2 (pregnant mice infected with E. coli + E. scaber 75% and S. androgynus 25%), P3 (pregnant mice infected with E. coli + E. scaber 50% and S. androgynus 50%), P4 (pregnant mice infected with E. coli + E. scaber 25% and S. androgynus 75%), P5 (pregnant mice infected with E. coli + S. androgynus 100%). Flow cytometry analysis was used to analyze cell populations expressing CD4+TNFα+, CD4+IFNγ+ and regulatory T cells (CD4+CD25+CD62L+). All treatment groups significantly (p<0.05) decreased TNFα and IFNγ levels, while the P2 group was more effective in increasing regulatory T cells at the 1st and 2nd trimesters of the pregnancy. This study showed that E. scaber and S. androgynus combination alleviated inflammation by reducing inflammatory cytokines (TNFα and IFNγ) and increasing T-regulatory cells. Therefore, E. scaber and S. androgynus combination could suppress the inflammation during pregnancy and infection.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115606788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Curcuma mangga Ethanol Extract Improves Sperm Quality of Mice Exposed to Monosodium Glutamate","authors":"S. Aini, A. Marhendra, Sri Rahayu","doi":"10.21776/ub.jels.2022.012.03.04","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.04","url":null,"abstract":"The current study aimed to determine variations effect in the dose of Curcuma mangga ethanol extract on male mice exposed to monosodium glutamate (MSG). The 24 adult male mice with the body weight of 25-30 g and 3-4 months old were used, then divided into six groups (n= four per group). The groups are K- (untreated mice), K+ (induced MSG) for 45 days, P1 (MSG + C. mangga extract 25 mg.g-1 BW), P2 (MSG + C. mangga extract 50 mg.g-1 BW); P3 (MSG + C. mangga extract 75 mg.g-1 BW); and P5 (MSG + C. mangga extract 100 mg.g-1 BW) for 30 days. Animals were dissected on the 46th day. The epididymis was isolated, and sperm quality was evaluated. The results showed that25-75 mg.g-1 BW C. mangga ethanolic extract could increase sperm viability by 24-29%, while at a dose of 25-100 mg.g-1 BW the sperm motility decreased by 58-92%. The administration of 100 mg.g-1 BW C. mangga could decrease it by 58% and concentration at a dose of 100 mg.g-1 BW increased sperm concentration by 96%. The use of MSG can increase sperm abnormalities compared to controls. This study showed that C. mangga effectively improved the sperm quality of mice exposed to MSG.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129392620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
O. Orole, A. Chuku, K. Olawuyi, E. Mantu, Alexander J. Philips, C. Okoli
{"title":"Microbial Agents in Terrorism, Biomarkers, and Public Health Challenges","authors":"O. Orole, A. Chuku, K. Olawuyi, E. Mantu, Alexander J. Philips, C. Okoli","doi":"10.21776/ub.jels.2022.012.03.09","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.09","url":null,"abstract":"Bioterrorism agents are mostly microorganisms with the capacity to deal explosive and lethal harm to humans, animals, and food crops. These microorganisms spread in the form of gases, whole organisms, or products of secondary metabolism of microorganisms. Classification of the agents is into three categories based on the ease of dissemination and end effects on a living population. While most health institutions are equipped to take care of sick people and treat suspected cases of infections, these institutions lack basic means of identifying bioterrorism acts. Special diagnostic equipment to identify causal organisms or agents is not available. Lack of training on what to do when terrorists strike using biological agents can cumulatively increase the lethal effects of such agents. Molecular techniques of identifying microorganisms to species level are as promising as they are time-consuming, while technical expertise and a conducive environment for managing such equipment are mostly not available in the African setting. The governments in Africa as a matter of urgency should provide an atmosphere where the teaming population of people without jobs are employed, while hospitals are adequately equipped, and training of health workers on what to do immediately after cases of terrorism are reported. The review highlights these agents and the diagnostic tools necessary to facilitate early response to bioterrorism.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128943409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}