The Journal of Experimental Life Sciences最新文献_第3页

Aquatic Environmental Analysis of the Hematological Profile of Barbonymus altus in the Brantas River, Jombang, East Java 东爪哇省仲邦市布兰塔斯河中阿尔图Barbonymus altus血液学特征的水生环境分析

The Journal of Experimental Life Sciences Pub Date : 2022-10-31 DOI: 10.21776/ub.jels.2022.012.03.07

Aang Setyawan Anjasmara, A. Hertika, U. Yanuhar

{"title":"Aquatic Environmental Analysis of the Hematological Profile of Barbonymus altus in the Brantas River, Jombang, East Java","authors":"Aang Setyawan Anjasmara, A. Hertika, U. Yanuhar","doi":"10.21776/ub.jels.2022.012.03.07","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.07","url":null,"abstract":"The Brantas River is the longest in East Java. One of the areas included in the flow of the Brantas River is the Jombang Regency. The Jombang Brantas River is used by the community to meet their daily needs in agriculture, plantations, industry, and fishing grounds. The input of waste generated from several community activities will result in the survival of fish in the waters. Fish as a biomarker agent is quite significant in estimating the level of heavy metal pollution. Red Bader Fish (Barbonymus altus) is often found and lives around the Jombang Brantas River. This study aims to determine the condition of the aquatic environment by observing the health of the fish through hematological observations of the Red Bader Fish (B. altus). Sampling was carried out at three stations with three replications every two weeks, which was carried out in February – March 2022 in the Jombang Brantas River. Data analysis used the Canonical Correspondence Analysis (CCA) method as an analytical method to determine the correlation between the dependent variable (hematological profile) and the independent variable (water quality conditions). In this study, the results showed that the water quality at Station 1 and Station 2 was classified as normal, while at Station 3, it was classified as polluted. Data analysis showed that erythrocytes, hemoglobin, and hematocrit had a relationship with temperature, pH, DO, BOD, ammonia, TSS, and TDS in low concentrations. Meanwhile, leukocytes and micronuclei showed a relationship with high concentrations of ammonia, BOD, TDS, and TSS.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116669327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring YidR Gene from Klebsiella pneumoniae To Design a Multi-epitope Vaccine Using Immunoinformatics Approach 利用免疫信息学方法探索肺炎克雷伯菌YidR基因设计多表位疫苗

The Journal of Experimental Life Sciences Pub Date : 2022-10-31 DOI: 10.21776/ub.jels.2022.012.03.08

Maulana Wildan Seputra, Hani Susiati, T. Raras

引用次数: 0

Isolation and Characterization of α-Amylase Enzyme on Brown Planthopper (Nilaparvata lugens Stal) On Rice 水稻褐飞虱α-淀粉酶的分离与鉴定

The Journal of Experimental Life Sciences Pub Date : 2022-10-31 DOI: 10.21776/ub.jels.2022.012.03.02

Muhammad Yusuf Abdan Syakuro, N. Haryadi, Wildan Muhlison, Irwanto Sucipto

{"title":"Isolation and Characterization of α-Amylase Enzyme on Brown Planthopper (Nilaparvata lugens Stal) On Rice","authors":"Muhammad Yusuf Abdan Syakuro, N. Haryadi, Wildan Muhlison, Irwanto Sucipto","doi":"10.21776/ub.jels.2022.012.03.02","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.03.02","url":null,"abstract":"Brown leafhopper (Nilaparvata lugens Stal) is an important pest in Indonesia that causes heavy losses. The rice was damaged by N. Lugens sucking plant liquid in the form of carbohydrates in the form of starch. Environmentally friendly control methods are needed to reduce the increasingly high use of inorganic pesticides. Control of N. lugens with biotechnology can be done by inhibiting the metabolic cycle in these insects. This method utilizes the proteins available in rice seeds to stop the performance of enzyme α-amylase in the digestive system of N. lugens. α-amylase is an enzyme that plays a role in the process of starch degradation so that it becomes a simpler form both in microorganisms. The characterization of α-amylase enzymes in insects is an important first step to the determination of appropriate protein inhibitors so that they can be used to produce pest-resistant Genetically Modified Organisms crops. The research was conducted in the Agrotechnology laboratory, University of Jember. The research began with isolation and purification of α enzymes from brown stems and then continued with testing of α-amylase activity. Test parameters include the effect of temperature, pH, and substrate concentration on the activity of the α-amylase enzyme. The results showed that supernatant extracted from brown planthoppers produces a clear zone in the agar medium, which means the activity of enzymes α-amylase in the hydrolysis of starch. The pH value of 6 provides the most optimum conditions for the activity of α enzymes. The α-amylase enzyme is able to work optimally in the temperature range of 30°C - 45°C, and experiences a decrease in activity when the temperature reaches 50°C. The α-amylase enzyme shows the ability to hydrolyze the amylase substrate to a concentration of 0.8 μg.μL-1.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"10 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134320388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Isolation and Identification of Nitrogen-Fixing Rhizobacteria associated with Cocoa plantation (Theobroma cacao L) as Biofertilizer Agent 与可可种植园(Theobroma cacao L)相关的固氮根细菌的分离与鉴定

The Journal of Experimental Life Sciences Pub Date : 2022-10-31 DOI: 10.21776/ub.jels.2022.012.03.01

Nurul Afiyatul Jannah, I. Mustafa, Y. Jatmiko

引用次数: 0

Phaleria macrocarpa Leaves Extract Reduce Tumors Growth and Improve Histological Changes of Liver and Kidney on 4T1 Breast Cancer Mice Model 大阳泻叶提取物抑制4T1乳腺癌小鼠肿瘤生长，改善肝、肾组织学变化

The Journal of Experimental Life Sciences Pub Date : 2022-06-30 DOI: 10.21776/ub.jels.2022.012.02.02

Lela Aminullah, Y. Christina, Muhaimin Rifa’i, M. S. Djati

引用次数: 0

Analysis of Microplastics in Water and Biofilm Matrices in Lahor Reservoirs, East Java, Indonesia 印尼东爪哇拉合尔水库水和生物膜基质中的微塑料分析

The Journal of Experimental Life Sciences Pub Date : 2022-06-30 DOI: 10.21776/ub.jels.2022.012.02.03

Putri Rahayu Pertiwi, M. Mahmudi, Zulkisam Pramudia, A. Kurniawan

{"title":"Analysis of Microplastics in Water and Biofilm Matrices in Lahor Reservoirs, East Java, Indonesia","authors":"Putri Rahayu Pertiwi, M. Mahmudi, Zulkisam Pramudia, A. Kurniawan","doi":"10.21776/ub.jels.2022.012.02.03","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.02.03","url":null,"abstract":"One of the aquatic ecosystems that are very susceptible to contamination is the reservoir ecosystem. Reservoirs have different characteristics from other water bodies because they receive continuous water input from the river that flows through them. The river water contains organic and inorganic materials that can cause pollution caused by various kinds of human activities. The pollutant that is currently getting more attention is microplastics. Hence, various ways are developed to monitor the presence of microplastics in environments. The biotic component that may adsorb and accumulate microplastics is microbes that formed biofilm matrices as a predominant habitat. This study analyzes the presence of microplastics in water and biofilm matrices in Lahor Reservoir. The water parameters (pH, dissolved oxygen, temperature, and flow velocity) were also measured. Samplings were carried out at three stations, namely station A (inlet channel), station B (middle), and station C (outlet channel). This study revealed that water quality parameters were still relatively good according to the environment's quality standards. The average value of microplastic abundance in water at station A was 0.0013 particles.mL -1 , station B was 0.00083 particles.mL -1 , and station C was 0.00072 particles.mL -1 . The average abundance of microplastics in the biofilm at station A, station B, and station C was 7.55 particles.g -1 , 7.26 particles.g -1 , and 4.59 particles.gram -1 , respectively. This study indicates that the abundance of microplastics in the biofilm in the Lahor Reservoir was thousands of times higher than in the water. According to the results of this study, the biofilm can be used as a biological agent in monitoring the presence of microplastics in aquatic ecosystems such as the Lahor Reservoir, East Java, Indonesia.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131106788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation and Identification of Rhizosphere Bacteria Associated with Taro (Colocasia esculenta L. Schott) as Plant Growth Promoting Rhizobacteria 促进植物生长的芋头根际细菌的分离与鉴定

The Journal of Experimental Life Sciences Pub Date : 2022-06-30 DOI: 10.21776/ub.jels.2022.012.02.01

Feybe Makitalentu, I. Mustafa, S. Suharjono

{"title":"Isolation and Identification of Rhizosphere Bacteria Associated with Taro (Colocasia esculenta L. Schott) as Plant Growth Promoting Rhizobacteria","authors":"Feybe Makitalentu, I. Mustafa, S. Suharjono","doi":"10.21776/ub.jels.2022.012.02.01","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.02.01","url":null,"abstract":"Taro is one of the food crops that has the potential to maintain food security. It has a high carbohydrate content and nutrients with a low glycemic index value. Taro is widely cultivated with intercropping techniques, especially in Kedungkandang District, Malang City. However, this technique caused a decrease in talas tubers production with only 710 tons ha-1. This number was lower than the total optimal production with 20.7 tons ha-1 of taro tubers. Plant Growth Promoting Rhizobacteria (PGPR), one of the most potential biofertilizers, can solve this problem with abilities such as phosphate-solubilizing, production of IAA, and nitrogen fixation. This research aimed to analyze the potency and identify the species of taro rhizosphere bacteria that had the best ability as PGPR agents. Taro rhizosphere bacteria were isolated using Pikovskaya medium and TSA (Tryptic Soy Agar) respectively. The PGPR abilities were evaluated on the phosphate-solubilizing, production of IAA, and nitrogen fixation. A total of 12 isolates for phosphate-solubilizing bacteria and ten isolates of IAA-producing bacteria. The highest concentration of phosphate solubilization was P1 isolate, with the concentration of 6.8 μg.mL-1; while I4 isolate had the highest potency for IAA production with the concentration of 23.11 μg.mL-1. Isolates P1 and I4 were selected for the nitrogen fixation ability test. P1 isolate shows the highest ammonia concentration of 2.52 μg.mL-1, and it was identified as Stenotrophomonas rhizophila with a similarity of 99.91% with Stenotrophomonas rhizophila R2A2 67. The potential isolates can be used as PGPR agents or biofertilizers to increase the production of taro plants.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123517032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Active Detergent Ingredients on Successful Fertilization and Embryo Development of Sea urchins Tripneustes gratilla (Linnaeus, 1758) 活性洗涤剂成分对海胆成功受精和胚胎发育的影响(Linnaeus, 1758)

The Journal of Experimental Life Sciences Pub Date : 2022-06-30 DOI: 10.21776/ub.jels.2022.012.02.04

Aprilia Paskarani Molle, A. Marhendra, Sri Rahayu

引用次数: 0

Expression of IL-17 on Breast Cancer Mice Treated by Combination of Phyllanthus Urinaria and Catharanthus roseus Extract 余甘子与花楸提取物联合治疗乳腺癌小鼠IL-17的表达

The Journal of Experimental Life Sciences Pub Date : 2022-06-30 DOI: 10.21776/ub.jels.2022.012.02.05

Aya Shofiyah, M. S. Djati, Muhaimin Rifa’i

引用次数: 0

Evaluating Arrowroot Starch Modification and Application in Wet Noodles 芋粉改性及其在湿面中的应用

The Journal of Experimental Life Sciences Pub Date : 2022-02-28 DOI: 10.21776/ub.jels.2022.012.01.01

Aprilia Bertha Puspitasari, G. J. Manuhara, D. Fibri, D. Muhammad

{"title":"Evaluating Arrowroot Starch Modification and Application in Wet Noodles","authors":"Aprilia Bertha Puspitasari, G. J. Manuhara, D. Fibri, D. Muhammad","doi":"10.21776/ub.jels.2022.012.01.01","DOIUrl":"https://doi.org/10.21776/ub.jels.2022.012.01.01","url":null,"abstract":"Increasing the Resistant Starch (RS) level in food products containing naturally high starch content is important as RS has been acknowledged as a functional food ingredient. The purpose of this study is to determine the effect of steamcooling treatment on the characteristic of arrowroot starch and to investigate the feasibility of arrowroot-based RS application on wet noodles. The study used a Completely Randomized Design (CRD) with one factor which was the steam-cooling cycle. Later, the substitution of 5%, 10%, and 20% of modified arrowroot starches on wet noodles was used, and then the consumer test was carried out. The results showed that the RS content of arrowroot starch remained after steam-cooling treatment. The treatment, however, reduced the water content and the brightness of the starch. Subsequently, arrowroot starch substitution without or with modification also reduced the lightness and tensile strength of wet noodles. Also, wet noodles with modified arrowroot starch substitution were significantly different in color, taste, and aroma parameters but were still acceptable to the panelists at a maximum substitution of 20%. The formulated wet noodles with modified arrowroot starch contained RS of 34.02% (d/b), and therefore they can be categorized as foodstuffs with high RS levels.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130427734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0