The Journal of Experimental Life Sciences最新文献_第10页

The Morphological Characters of The Malayan Pit Viper Calloselasma rhodostoma (Kuhl, 1824): on The Cephalic Scalation and Distribution Status in Indonesia 马来亚坑蝰Calloselasma rhodostoma (Kuhl, 1824)的形态特征:关于印度尼西亚头部鳞片和分布状况

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.10

Ahmad Muammar Kadafi, A. Hamidy, N. Kurniawan

引用次数: 0

Proximate Composition of Some Common Alternative Flour as Fish Feed Ingredient 几种常见替代面粉作为鱼饲料原料的近似组成

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/ub.jels.2018.008.03.12

Arrum Nurjannah Herdiyanti, H. Nursyam, A. Ekawati

{"title":"Proximate Composition of Some Common Alternative Flour as Fish Feed Ingredient","authors":"Arrum Nurjannah Herdiyanti, H. Nursyam, A. Ekawati","doi":"10.21776/ub.jels.2018.008.03.12","DOIUrl":"https://doi.org/10.21776/ub.jels.2018.008.03.12","url":null,"abstract":"Indonesia has very large territorial water, so it supports the development of the economy in the field of fisheries, one of which is through aquaculture. Recently fish meal has become the most expensive protein ingredient in aquaculture feeds. We aim to proximately analysis of some common alternative flour as fish feed ingredient i.e. fish flour, shrimp head flour, soybean flour, and bran flour to get best-recommended flour for type cultured fish. Fish flour, shrimp head flour, soybean flour, and bran flour was purchased from the fish farmer in Malang who use alternative fish feed flour for their aquaculture. Proximate analysis carried out by protein content, Moisture content, Fat and Fiber. Proximate analysis was conducted in the Fishery Products Engineering laboratory, Brawijaya University on April 2018. The results showed that shrimp head flour had higher protein content than others flour that is about 48.71%, fat and carbohydrate content about 7.99%, and 18.34% respectively. Soybean flour contains protein, fat and carbohydrate about 31.29%, 20.75%, and 30.45% respectively. Carnivorous fish culture can use high protein flour as an alternative ingredient for feed such as fish flour and shrimp head flour; balance proximate composition for omnivorous fish cultures such as soy flour and high carbo flour such as bran flour for herbivorous fish culture. Keywords: Bran flour , Shrimp head flour, Soybean flour, Proximate analysis.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129711909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica 大田菁、山六甲和芡粉复合草药的抗高血糖和免疫调节作用

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.09

Ahmad Zamroni, E. Zubaidah, M. Rifa’i, S. Widjanarko

{"title":"Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica","authors":"Ahmad Zamroni, E. Zubaidah, M. Rifa’i, S. Widjanarko","doi":"10.21776/UB.JELS.2018.008.03.09","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.09","url":null,"abstract":"Diabetes has become a serious global public health problem due to its high prevalence and mortality. Unfortunately, current anti-diabetic drugs are having some limitations and adverse effects. Therefore, searching for a new anti-diabetic agent is an urgent challenge. In this research, we examined the effectiveness of a traditional anti-diabetic polyherbal composed of Sesbania grandiflora seeds, Salacca zalacca leaves and Acalypha indica roots (2:1:1). The study was aimed to explore the anti-hyperglycemic effect of the polyherbal in STZ-induced diabetic mice and to investigate the immunomodulatory activity involved in the process of controlling hyperglycemia. Our results showed that the polyherbal water extract (150 mg.kgBW -1 ) could suppress blood glucose elevation and preserve pancreatic islet of diabetic mice. Moreover, the polyherbal treatment could normalize the relative amount of activated CD4 + CD62L - and CD8 + CD62L - T cells. The polyherbal extract also stimulated the production of CD4 + CD25 + FoxP3 + regulatory T cells (Tregs) which is known to play an important role in diabetes control. In addition, polyherbal treatment also increased the relative amount of anti-inflammatory cytokines IL-10 and TGF-β. These results revealed that the polyherbal extract has an anti-hyperglycemic and immunomodulatory activity that may provide beneficial function in diabetes healing. Keywords: Antidiabetes, Immunomodulator, Polyherbal, Regulatory T cell.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125051221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) 印度楝叶乙醇提取物对罗非鱼的免疫刺激作用

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/ub.jels.2018.008.03.08

R. Putri, S. Andayani, A. Yuniarti

{"title":"Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus)","authors":"R. Putri, S. Andayani, A. Yuniarti","doi":"10.21776/ub.jels.2018.008.03.08","DOIUrl":"https://doi.org/10.21776/ub.jels.2018.008.03.08","url":null,"abstract":"Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf ( A zadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus . In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf ( A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves ( A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf ( A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 10 4 cells.mL -1 . Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130228721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer) Hsp70和β-肌动蛋白基因的表达对亚洲海鲈病毒性神经坏死的免疫应答

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.02

Y. Wahyudi, U. Yanuhar, Maftuch Maftuch

{"title":"Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer)","authors":"Y. Wahyudi, U. Yanuhar, Maftuch Maftuch","doi":"10.21776/UB.JELS.2018.008.03.02","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.02","url":null,"abstract":"The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass ( L. calcalifer ) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass ( L. calcalifer ) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123183553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat’s Spermatozoa after Freezing at -80°C 添加桑椹提取物稀释三卵黄对-80℃冷冻山羊精子异常的影响

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.03

Apriani Herni Rophi, Sri Rahayu, G. Ciptadi

{"title":"The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat’s Spermatozoa after Freezing at -80°C","authors":"Apriani Herni Rophi, Sri Rahayu, G. Ciptadi","doi":"10.21776/UB.JELS.2018.008.03.03","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.03","url":null,"abstract":"The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P 0 ), 10% (P 1 ), 20% (P 2 ), and 30% (P 3 ). The result showed that the percentage of sperms abnormality in P 0 , P 1 , P 2 , and P 3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P 2 was the lowest percentage, while P 3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125792903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Isolation and Identification of Antagonistic Bacterium against a Pathogens of Bacterial Tuber Rot of Amorphophallus muelleri 一株魔芋块茎腐病病原菌的分离与鉴定

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.06

Nurfitri Arfani, R. Azrianingsih, S. Suharjono

引用次数: 2

Numerical Simulation of Leslie-Gower Predator-Prey Model with Stage-Structure on Predator 捕食者上具有阶段结构的Leslie-Gower捕食者-食饵模型的数值模拟

The Journal of Experimental Life Sciences Pub Date : 2018-10-30 DOI: 10.21776/UB.JELS.2018.008.03.011

R. Pratiwi, A. Suryanto, T. Trisilowati

引用次数: 0

Comparing Vector-host and SEIR models for Zika Virus Transmission 寨卡病毒传播媒介-宿主和SEIR模型的比较

The Journal of Experimental Life Sciences Pub Date : 2018-10-08 DOI: 10.21776/UB.JELS.2018.008.03.05

Puji Andayani, Rizal Dian Azmi, L. R. Sari

引用次数: 1

Dynamical Analysis of Predator-Prey Model Leslie-Gower with Omnivore 杂食动物捕食-食饵模型的动力学分析

The Journal of Experimental Life Sciences Pub Date : 2018-07-26 DOI: 10.21776/UB.JELS.2018.008.02.10

Rina Exviani, W. M. Kusumawinahyu, Noor Hidayat

引用次数: 0