The Journal of Experimental Life Sciences最新文献_第9页

Role of Active Compounds of Bohadschia argus Inhibit Cancer Cell Survival 黑鲈活性成分对肿瘤细胞存活的抑制作用

The Journal of Experimental Life Sciences Pub Date : 2019-03-22 DOI: 10.21776/UB.JELS.2019.009.01.03

Jantje Wiliem Souhaly, Sri Rahayu, W. Widodo

引用次数: 1

Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period 紫穗槐休眠结束时茎尖分生组织的解剖特征

The Journal of Experimental Life Sciences Pub Date : 2019-03-22 DOI: 10.21776/UB.JELS.2019.009.01.04

Muhammad Naufal Afifi, N. Harijati, R. Mastuti

{"title":"Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period","authors":"Muhammad Naufal Afifi, N. Harijati, R. Mastuti","doi":"10.21776/UB.JELS.2019.009.01.04","DOIUrl":"https://doi.org/10.21776/UB.JELS.2019.009.01.04","url":null,"abstract":"Porang is one species member of the genus Amorphophallus . In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium. Keywords : Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125413514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Antifeedant Effect of Moringa oleifera (L.) Leaf and Seed Extract on Growth and Feeding Activity of Spodoptera litura (Fab.) (Lepidoptera: Noctuidae) 辣木的拒食作用叶片和种子提取物对斜纹夜蛾生长和取食活性的影响(鳞翅目:夜蛾科)

The Journal of Experimental Life Sciences Pub Date : 2019-03-22 DOI: 10.21776/UB.JELS.2019.009.01.05

Amelia Tridiptasari, A. Leksono, D. Siswanto

引用次数: 2

Transoceanic Disperse of the White-lipped Island Pit Viper (Cryptelytrops insularis; Kramer,1997) from Sundaland to Lesser Sunda, Indonesia 白唇岛蝮蛇的越洋分布Kramer,1997)从巽他岛到印度尼西亚的小巽他岛

The Journal of Experimental Life Sciences Pub Date : 2019-03-22 DOI: 10.21776/UB.JELS.2019.009.01.02

B. Priambodo, F. G. Liu, N. Kurniawan

{"title":"Transoceanic Disperse of the White-lipped Island Pit Viper (Cryptelytrops insularis; Kramer,1997) from Sundaland to Lesser Sunda, Indonesia","authors":"B. Priambodo, F. G. Liu, N. Kurniawan","doi":"10.21776/UB.JELS.2019.009.01.02","DOIUrl":"https://doi.org/10.21776/UB.JELS.2019.009.01.02","url":null,"abstract":"White-lipped island pit viper ( Cryptelytrops insularis ) is one of the most distributed Viperidae in Indonesia, especially in eastern part of Sundaland and Lesser Sunda. To investigate the evolutionary history and the dispersal pattern of C. insularis, we collected 23 samples from 11 localities. Four simultaneous genes composing two mitochondrial genes (16S rRNA & ND4) and two nuclear genes (7IβFIB & 3ITBP) have been successfully amplified and sequenced. Bayesian inference was performed to reconstruct the phylogeny tree. Furthermore, time divergence and the population demography analyses were estimated. The phylogeny tree of C. insularis exhibits monophyletic group, with four geographically structured lineages. The time divergence estimation indicated that C. insularis evolved at approximately 7 million years ago (mya). Population demography was inferred by Bayesian Skyline Plot analysis, it shows that the population increased constantly from the past to recent time. The evolutionary history of C. insularis can be explained by a pattern of the time divergences estimation that indicating movement from West (Java) to East (Lesser Sunda). We expected that the dispersal factor of C. insularis into many different islands (in Lesser Sunda) is caused by the animal helped and also oceanic rafting which could be the stepping stones to another island.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130678529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume 贮藏地点和时间对魔芋块茎品质的影响

The Journal of Experimental Life Sciences Pub Date : 2019-02-22 DOI: 10.21776/UB.JELS.2019.009.01.06

Putri Rabiah Al Adawiah, R. Azrianingsih, R. Mastuti

{"title":"Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume","authors":"Putri Rabiah Al Adawiah, R. Azrianingsih, R. Mastuti","doi":"10.21776/UB.JELS.2019.009.01.06","DOIUrl":"https://doi.org/10.21776/UB.JELS.2019.009.01.06","url":null,"abstract":"Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System , odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers. Keywords: Physical change, porang tubers, storage time.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126489076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam 基于基因细胞色素氧化酶C亚基I (COI)的松古鲁坝Mystacoleucus属的分子鉴定和遗传特征

The Journal of Experimental Life Sciences Pub Date : 2019-02-22 DOI: 10.21776/UB.JELS.2019.009.01.07

F. S. Valen, M. Widodo, Y. Kilawati

{"title":"Molecular Identification and Genetic Characteristics of Genus Mystacoleucus Based on Gene Cytochrome Oxidase C Subunit I (COI) in Sengguruh Dam","authors":"F. S. Valen, M. Widodo, Y. Kilawati","doi":"10.21776/UB.JELS.2019.009.01.07","DOIUrl":"https://doi.org/10.21776/UB.JELS.2019.009.01.07","url":null,"abstract":"In general, the process of naming several species of fish including species from the genus Mystacoleucus is still carried out based on morphological characters, which are often found in almost the same morphological characters in different fish species. Therefore, it is necessary to apply a more accurate identification method, which is a DNA-based identification method called DNA barcoding. The purpose of this study was to identify the species of the genus Mystacoleucus on the Sengguruh Dam molecularly based on the Cytochrome Oxidase C Subunit I (COI) gene. A caudal fin was taken on the test fish and preserved in 95% ethanol solution for molecular identification. The results of the identification showed that the sample belonged to the Mystacoleucus marginatus species with Identity values between 99-100% and E-value 0.0. The data obtained showed that from the calculation of genetic distance presented in the form of data matrix and phylogenetic tree reconstruction, there were 2 species that had a far genetic distance from the M . marginatus sample from the Sengguruh Dam namely M . atridorsalis with the furthest genetic distance of 0.1932-0.2595 and M . lepturus with genetic distance between 0.1117-0.1193. However, there are one species that has the closest distance, M . padangensis with genetic distance between 0.0019-0.0038 and identity values up to 99%. Keywords: DNA barcode, Molecular identification, Mystacoleucus , Phylogenetic COI.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127945078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Phytoremediation of Lead-Contaminated Soil by Using Vetiver Grass (Vetiveria zizanioides L.) 香根草修复铅污染土壤的研究

The Journal of Experimental Life Sciences Pub Date : 2019-02-22 DOI: 10.21776/UB.JELS.2019.009.01.09

R. S. Abdallah, A. Rachmansyah, B. Yanuwiadi

引用次数: 1

The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid 在-80°C冷冻条件下添加Tris稀释液孵育卵黄和羊水对山羊精子的影响

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.01

L. Nisa, Sri Rahayu, G. Ciptadi

{"title":"The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid","authors":"L. Nisa, Sri Rahayu, G. Ciptadi","doi":"10.21776/UB.JELS.2018.008.03.01","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.01","url":null,"abstract":"This study aims to determine the combination of the hatching egg yolk (HEY) and amniotic fluid (AF) in Tris diluent to the type of goat spermatozoa abnormalities after frozen at temperature -80°C. This study used four treatments and 6 replications. The semen is diluted using tris extender and added treatment, there are control group (T 0 ) (Tris egg yolk 20%), T 1 (15% HEY + 5% AF), T 2 (10% HEY + 10% AF), and T3 (5% HEY + 15% AF). After dilution, the semen was equilibrated for 2 hours, then stored using Mr. Frosty and frozen at temperature -80°C in the ultra-freezer for 24 hours. The type of spermatozoa abnormalities type was evaluated on fresh semen and after thawing. The data were analyzed using ANOVA (P<0.05). The results showed that there is no significant difference among treatments. The primary abnormalities in fresh semen, there were macro-cephalic, amorphous, and the secondary spermatozoa abnormalities such as detached head, mid-piece defect, coiled tail, loosehead, shoe-hook tail, and a broken tail. The secondary abnormalities in post-thawing were found both in the control group and treatment, which are detached head, loosehead, coiled tail, shoe-hook tail, and broken tail. The conclusion of this study was the percentage of secondary abnormalities after thawing was increasing both in the control and treatment groups. Keywords: Amniotic Fluid, Hatching Egg Yolk, Mr. Frosty , Type of Spermatozoa Abnormality.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123321372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria Tidore岛丁香促进植物生长的根际细菌的筛选

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.04

I. Ishak, T. Ardyati, L. Q. Aini

{"title":"Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria","authors":"I. Ishak, T. Ardyati, L. Q. Aini","doi":"10.21776/UB.JELS.2018.008.03.04","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.04","url":null,"abstract":"Tidore Island in North Maluku Province is one of the clove ( Syzygium aromaticum ) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 μgL -1 ), phosphate solubilization (8.37 μgL -1 ), and ammonium production (11.71 μgL -1 ), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia , and Stenotrophomonas. Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129676617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District 帕苏鲁区凡纳滨对虾传统养殖池白斑综合征病毒检测

The Journal of Experimental Life Sciences Pub Date : 2018-10-31 DOI: 10.21776/UB.JELS.2018.008.03.07

A. Usman, S. Andayani, E. Y. Herawati

{"title":"White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District","authors":"A. Usman, S. Andayani, E. Y. Herawati","doi":"10.21776/UB.JELS.2018.008.03.07","DOIUrl":"https://doi.org/10.21776/UB.JELS.2018.008.03.07","url":null,"abstract":"Vanamei shrimp ( Litopenaeus vannamei ) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname ( L. vannamei ) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms ( L. vannamei ) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%. Keywords: PCR, Prevalence, Shrimp, Vannamei , WSSV.","PeriodicalId":177289,"journal":{"name":"The Journal of Experimental Life Sciences","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131604891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1