JURNAL ILMIAH SAINS最新文献

{"title":"Antioxidant Activity of Dewandaru Leaf (Eugenia Uniflora L.) Ethanol Extract and Determination of Total Flavonoid and Phenolic Content","authors":"Khoirul Anwar, Faridha Maera Lokana, A. Budiarti","doi":"10.35799/jis.v22i2.43913","DOIUrl":"https://doi.org/10.35799/jis.v22i2.43913","url":null,"abstract":"Dewandaru (Eugenia uniflora L.) is a plant that has antioxidant activities. The compounds contained phenolic compounds and flavonoids.  This study aimed to test the antioxidant activity of the ethanol extract of Dewandaru leaves (Eugenia uniflora L.) with ABTS method and determine the content of flavonoid and total phenolic compounds. Ethanol extract of Dewandaru leaves was obtained by maceration using 96% ethanol. The ethanol extract of Dewandaru leaves identified the active compounds by FTIR (Fourier Transform Infra Red) and phytochemical screening. Ethanol extract of Dewandaru leaves was tested for its antioxidant activity using the ABTS method to obtaine the IC50 value and determined total flavonoid and phenolic content using UV/Vis spectrophotometry to obtain the levels (mg/g sample). The identification results show that the ethanol extract of Dewandaru leaves is a flavonoid compound of the dihydroflavonol type which has a bound OH functional group, aliphatic CH, C = O, C = C aromatic, C - O and aliphatic CH, and there are hydroxy groups on the C-3, C atoms. -5, and C-7. The results of phytochemical screening showed that the ethanol extract of Dewandaru leaves contains saponins, tannins, phenolics, and flavonoids. The ethanol extract of Dewandaru leaves has antioxidant activity with an IC50 value of 33.92 ppm and trolox with an IC50 value of 19.24 ppm. Based on the IC50 value, both are categorized very strong antioxidant activity. The total phenolic and total flavonoid levels were 327.52 mgGAE/g and 35.65 mgQE/g.\u0000Keywords: Antioxidant; Eugenia uniflora L.; flavonoid; phenolic\u0000Aktivitas Antioksidan Ekstrak Etanol Daun Dewandaru (Eugenia Uniflora L.) serta Penetapan Flavonoid dan Fenolik Total\u0000ABSTRAK\u0000Dewandaru (Eugenia uniflora L.) termasuk salah satu tanaman yang mempunyai khasiat antioksidan. Senyawa yang terkandung didalamnya antara lain senyawa fenolik dan flavonoid. Penelitian ini bertujuan untuk melakukan uji aktivitas antioksidan ekstrak etanol daun dewandaru (Eugenia uniflora L.) menggunakan metode ABTS dan menetapkan kandungan senyawa flavonoid dan fenolik total. Ekstrak etanol daun dewandaru diperoleh dengan cara maserasi menggunakan etanol 96 %. Ekstrak etanol daun dewandaru diidentifikasi senyawa aktif dengan FTIR (Fourier Transform Infra Red) dan skrinning fitokimia. Ekstrak etanol daun dewandaru diuji aktivitas antioksidan menggunakan metode ABTS sehingga diperoleh nilai IC50. Selanjutnya ekstrak etanol daun dewandaru ditetapkan kandungan flavonoid dan fenolik totalnya secara spektrofometri UV/Vis hingga diperoleh kadarnya (mg/g sampel). Hasil identifikasi menunjukkan ekstrak etanol daun dewandaru mengandung senyawa golongan flavonoid jenis dihidroflavonol yang mempunyai gugus fungsi OH terikat, CH alifatik, C = O, C = C aromatik, C – O dan CH alifatik, serta terdapat gugus hidroksi pada atom C-3, C-5, dan C-7. Hasil skrining fitokimia  menunjukkan bahwa ekstrak etanol daun dewandaru mengandung senyawa saponin, tanin, fenolik, d","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77414699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fortification of Cocogurt Made From Coconut (Cocos nucifera L.) Milk With Yellow Yam (Ipomea batatas L.) Puree","authors":"Cenny Sulastri Br. Pandiangan, M. Sumual, L. Mandey","doi":"10.35799/jis.v22i2.43912","DOIUrl":"https://doi.org/10.35799/jis.v22i2.43912","url":null,"abstract":"This study aims to fortify yogurt from coconut milk (cocogurt) with yellow sweet potato puree and its effect on pH, viscosity, total lactic acid bacteria, and identify sensory properties of preference for taste, aroma, color, and texture. The method used in this research is the manufacture of yogurt starter, coconut milk, and yellow yam puree, the test parameters include acidity test (pH value), viscosity, total lactic acid bacteria and organoleptic test. This study used a completely randomized design (CRD) with 6 additional treatments of yellow yam puree: P0(0%), P1(5%), P2(10%), P3(15%), P4(20%), P5(25%). The results showed that fortification of cocogurt with yellow yam puree produced cocogurt with lower acidity (pH) (3.38-4.71), viscosity (4.46-6.85 cP) and total lactic acid bacteria (2,3 x 105 - 6.5 x 105 cfu/ml) is increasing. This study concluded that the best cocogurt treatment was found in the fortification of 5% yellow yam puree with a pH value of 4.39, viscosity 4.46 cP, total lactic acid bacteria 2.3 x 105 cfu/ml and sensory properties of preference for taste, aroma, color, texture shows the score of the results of the scale (6), namely likes.\u0000Keywords: cocogurt; coconut milk; yellow yam puree\u0000 Fortifikasi Cocogurt Santan Kelapa (Cocos nucifera L.) Dengan Puree Ubi Jalar Kuning (Ipomea batatas L.)\u0000ABSTRAK\u0000Penelitian ini bertujuan untuk melakukan fortifikasi yogurt dari santan kelapa (cocogurt) dengan puree ubi jalar kuning dan pengaruhnya terhadap pH, viskositas, total bakteri asam laktat, dan mengidentifikasi sifat sensoris tingkat kesukaan rasa, aroma, warna, dan tekstur. Metode yang digunakan dalam penelitian ini adalah pembuatan starter yogurt, santan kelapa, dan puree ubi jalar kuning. Parameter uji meliputi uji derajat keasaman (nilai pH), viskositas, total bakteri asam laktat serta uji organoleptik. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan 6 perlakuan penambahan puree ubi jalar kuning : P0(0%), P1(5%), P2(10%), P3(15%), P4(20%), P5(25%). Hasil penelitian menunjukan bahwa fortifikasi cocogurt dengan puree ubi jalar kuning menghasilkan cocogurt dengan derajat keasaman (pH) yang semakin rendah (4,71-3,38), viskositas (4,46-6,85 cP) dan total bakteri asam laktat (2,3 x 105-6,5 x 105 cfu/ml) semakin meningkat. Penelitian ini menyimpulkan perlakuan terbaik cocogurt terdapat pada fortifikasi puree ubi jalar kuning 5% dengan nilai pH 4,39, viskositas 4,46 cP, total bakteri asam laktat 2,3 x 105 cfu/ml dan sifat sensoris tingkat kesukaan rasa, aroma, warna, tekstur menunjukan skor hasil skala (6) yaitu suka.\u0000Kata kunci: cocogurt; puree ubi jalar kuning; santan kelapa","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79054160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of SWAT Model to predict Erosion and Sediment in the catchment area of Lake Tondano","authors":"J. Rombang, J. Kalangi, Meity R. Rantung","doi":"10.35799/jis.v22i2.43814","DOIUrl":"https://doi.org/10.35799/jis.v22i2.43814","url":null,"abstract":"The Use of SWAT Model to Predict Erosion and Sediment in the Catchment Area of Lake Tondano. The objective of this research is to assess the accuracy of SWAT model to predict erosion and sediment in the catchment area of lake Tondano. A comprehensive hydrological model of SWAT is one of several models available to predict erosion dan sediment load. The validation of this model would be tested and it would be acceptable if Nash-Sutcliffe model Efficiency (NSE) coefficient was equal or greater than 0.4. The results in this experiment showed that values of NSE coefficient between sediment load observed and predicted by SWAT were between 0.4 and 0.9. Therefore, the use of SWAT model to predict erosion dan sediment in Tondano lake catchment area is satisfying and very good.\u0000Keywords: Catchment area; erosion; sedimentation; SWAT; NSE\u0000 Penggunaan Model SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano\u0000 Abstrak\u0000Penggunaan Model SWAT untuk Prediksi Erosi dan Sedimen di Area Tangkapan Air Danau Tondano. Penelitian ini bertujuan menguji keakuratan model SWAT dalam menduga erosi dan sedimen di area tangkapan air danau Tondano. SWAT (Soil Water Assesment Tool) merupakan model hidrologi yang komprehensif karena mempertimbangkan kondisi biofisik, iklim dan manajemen area tangkapan air dalam prediksi erosi dan sedimen. Penggunaan model SWAT untuk pendugaan erosi dan sedimen di area tangkapan air danau Tondano dapat diterima apabila hasil validasi sedimen terukur dan sedimen pendugaan menunjukkan koefisien NSE (Nash-Sutcliffe model Efficiency) lebih besar atau sama dengan 0,4. Penelitian ini menunjukkan bahwa model SWAT bisa digunakan untuk prediksi erosi dan sedimentasi di area tangkapan air danau Tondano karena koefisien NSE yang diperoleh pada 4 sungai yang bermuara di danau Tondano berkisar antara 0,4 sampai 0,9. Dengan demikian, pendugaan erosi dan sedimen di area tangkapan air danau Tondano dengan model SWAT dapat memberikan hasil yang memuaskan sampai sangat baik.\u0000Kata kunci: Area tangkapan air; erosi; sedimentasi; SWAT; NSE","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91131422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial Activity of Secondary Metabolits Isolated from Mangrove Leaves Sonneratia alba","authors":"V. Dotulong, L. Damongilala, D. Wonggo, L. A. Montolalu","doi":"10.35799/jis.v22i2.42332","DOIUrl":"https://doi.org/10.35799/jis.v22i2.42332","url":null,"abstract":"Mangroves Sonneratia alb is a plants that grows in tidal areas with considerable environmental pressure. This condition causes the plant to synthesize secondary metabolites that have certain bioactivity to protect it self from destruction. The purpose of this study was to isolate antibacterial secondary metabolites from the mangrove leaves of  S.alba. The method applied was to isolate secondary metabolites from the ethyl setate fraction using column chromatography and identified the results of isolation with thin layer chromatography until a pure isolate was obtained. Pure isolates were characterized in structure using HNMR, IR and LC-MS spectroscopy. The resulting isolate in the form of methanol-soluble oil had the molecular formula C24H38O4 with the name Di(2-ethylhexyl)phthalate. The antibacterial activity of this compound was the same as the antibacterial activity of amoxicillin as a positive control, namely the diameter of the inhibition zone was 7 mm at a sample concentration of 0,21%.Aktivitas Antibakteri Metabolit Sekunder yang Diisolasi dari Daun Mangrove Sonneratia albaABSTRAKMangrove termasuk Sonneratia alba adalah tumbuhan yang tumbuh pada daerah pasang surut dengan tekanan lingkungan yang cukup besar, keadaan ini menyebabkan tumbuhan mangrove menyintesis metabolit sekunder yang mempunyai bioaktivitas tertentu untuk melindungi diri dari perusakan. Tujuan dari penelitian ini adalah mengisolasi metabolit sekunder antibakteri  dari daun mangrove  S.alba.. Metode yang digunakan adalah mengisolasi metabolit sekunder pada fraksi etil asetat menggunakan kromatografi kolom dan identifikasi hasil isolasi dengan kromatografi lapis tipis hingga didapatkan isolat murni. Isolat murni dikarakterisasi strukturnya menggunakan spektroskopi HNMR, IR dan LC-MS. Isolat yang dihasilkan berupa minyak yang larut dalam metanol mempunyai rumus molekul C24H38O4 dengan nama Di(2-etilhexil)fthalat. Aktivitas antibakteri dari senyawa ini  sama dengan aktivitas antibakteri amoxicilin sebagai kontrol positif yaitu diameter zona hambat sebesar 7 mm pada konsentrasi sampel 0,21%.Kata kunci: Aktivitas antibakteri; metabolit sekunder; Sonneratia alba","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90179520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Screening, Relationship of Total Phenolic with Antioxidant Activity Of Ethanol and Methanol Extracts of Kesambi (Schleichera oleosa (Lour.) Oken) Bark","authors":"Nur Alim, T. Hasan, R. Rusman, Jasmiadi Jasmiadi, Z. Zulfitri","doi":"10.35799/jis.v22i2.40091","DOIUrl":"https://doi.org/10.35799/jis.v22i2.40091","url":null,"abstract":"Phenolic content has a role in antioxidant activity. The higher the phenolic content of a test sample, the higher the antioxidant activity, which is indicated by a smaller IC50 value. Methanol and 70% ethanol are solvents that have been proven to be widely used to extract total plant phenolic compounds. This study aims to determine the phytochemical content and the relationship of total phenolic content with antioxidant activity of ethanol and methanol extract of Schleichera oleosa (Lour.) Oken) bark using UV-Vis spectrophotometry. Schleichera oleosa (Lour.) Oken was extracted by maceration using 70% ethanol and methanol as solvent. Qualitative analysis of phytochemical compounds using specific reagents, analysis of total phenolic content, and antioxidant activity test using the DPPH method using UV-Vis spectrophotometry at 764 nm and 515 nm, respectively. The results of the qualitative test showed that the ethanol and the methanol extract was positive for alkaloids, flavonoids, and phenolics. The results of the analysis of total phenolic content and antioxidant activity showed that the 70% ethanol extract had a total phenolic content of 7.6829 mg gallic acid equivalent/g with IC50 28.5240 ppm and the methanol extract had a total phenolic content of  9.2057 mg gallic acid equivalent/g with IC50 1.6191 ppm. From these results, it was concluded that the higher the total phenolic content, the higher the antioxidant activity.Keywords: Antioxidant; Schlechera oleosa (Lour.) Oken.; total phenolic Skrining Fitokimia dan Hubungan Kadar Fenolik Total dengan Aktivitas Antioksidan Ekstrak Etanol dan Metanol Kulit Batang Kesambi (Schleichera oleosa (Lour.) Oken)ABSTRAKKadar fenolik memiliki peran terhadap aktivitas antioksidan. Semakin tinggi kadar fenolik suatu sampel uji maka aktivitas antioksidannya juga semakin tinggi, yang ditunjukkan dengan nilai IC50 yang lebih kecil. Pelarut metanol dan etanol 70% merupakan pelarut yang telah terbukti banyak digunakan untuk menarik senyawa fenolik total tanaman.  Penelitian ini bertujuan untuk menentukan kandungan fitokimia, hubungan kadar fenolik total dengan aktivitas antioksidan ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) menggunakan spektrofotometri UV-Vis. Kulit batang kesambi (Schleichera oleosa (Lour.) Oken) diekstraksi dengan cara maserasi menggunakan pelarut etanol 70% dan pelarut metanol. Analisis kualitatif senyawa fitokimia menggunakan pereaksi spesifik, analisis kadar fenolik total dan uji aktivitas antioksidan dengan metode DPPH menggunakan spektrofotometri UV-Vis masing-masing pada panjang gelombang 764 nm dan 515 nm. Hasil uji kualitatif menunjukkan bahwa ekstrak etanol dan ekstrak metanol kulit batang kesambi (Schleichera oleosa (Lour.) Oken) positif mengandung alkaloid, flavonoid dan fenolik. Hasil analisis kadar fenolik total dan uji aktivitas antioksidan menunjukkan bahwa ekstrak etanol 70% memiliki kadar fenolik total sebesar 7,6829  mgGAE/g ekstrak d","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78583321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aktivitas Antioksidan Ekstrak dan Fraksi dari Daun Kamboja Putih (Plumeria alba L.) terhadap 1,1-Difenilpikrilhidrazin (DPPH) (Antioxidant Activity of Extracts and Fractions of White Cambodian Leaves (Plumeria alba L.) against 1,1-Diphenylpicrylhydrazine (DPPH))","authors":"Aloysius Barry Anggoro, Erlien Lusy Wijaya, N. Elisa","doi":"10.35799/jis.v22i2.43434","DOIUrl":"https://doi.org/10.35799/jis.v22i2.43434","url":null,"abstract":"Radikal bebas merupakan senyawa yang berperan dalam peningkatan resiko penyakit degeneratif yang mendominasi angka kematian di Indonesia. Antioksidan sebagai penangkal radikal bebas, salah satunya terdapat dalam daun kamboja. Daun kamboja putih dipilih karena relatif aman, ketersediaan yang melimpah, dan memiliki potensi sebagai antioksidan yang cukup menjanjikan. Penelitian ini bertujuan untuk menentukan aktivitas antioksidan dari ekstrak, partisi dan fraksi dari daun kamboja putih. Metode ekstraksi yang dipakai adalah remaserasi menggunakan pelarut metanol, dilanjutkan dengan partisi menggunakan pelarut n-heksana, etil asetat dan air. Partisi dengan IC50 paling rendah atau memiliki aktivitas antioksidan tertinggi selanjutnya difraksinasi dengan kromatografi kolom. Penentuan aktivitas antioksidan dengan metode DPPH dilakukan pada panjang gelombang 516,3 nm. Hasil penelitian menunjukkan bahwa partisi etil asetat memiliki IC50 terendah sebesar 94,43 ppm dibandingkan dengan partisi lainnya, sedangkan fraksi 2 memiliki daya antioksidan tertinggi diikuti fraksi 3 dan fraksi 1 dengan IC50 berturut-turut sebesar 54,11 ppm; 77,79 ppm dan 84,07 ppm.Kata kunci: Antioksidan; kamboja putih; metode DPPH; profil kromatografiABSTRACTFree radicals are compounds that play a role in increasing the risk of degenerative diseases that dominate mortality rates in Indonesia. Antioxidants as an antidote to free radicals, one of which is found in frangipani leaves. White frangipani leaves were chosen because they are relatively safe, abundantly available, and have promising potential as antioxidants. This study aims to determine the antioxidant effect of extracts, partitions, fractions from white frangipani leaves, and the thin layer chromatographic profile of the partition with the highest antioxidant activity. The extraction method being used was maceration using methanol followed by partioning the crude extract using n-hexane, ethyl acetate and water solvents. The partition with the lowest IC50 or having the highest antioxidant activity is then fractionated using column chromatography. The determination of antioxidant activity using DPPH method was performed at 516.3 nm. The results showed that  the ethyl acetate partition had the lowest IC50 among other partitions (94.43 ppm), whereas fraction 2 had the highest antioxidant effect which was followed by fraction 3 and fraction 1 with IC50 respectively, 54.11 ppm; 77.79 ppm and 84.07 ppm.Keywords: Antioxidant; chromatography profil; DPPH method; white frangipani","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89968209","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inventarisasi Tanaman Puring (Codiaeum variegatum L.) di Pekarangan Rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan, Provinsi Sulawesi Utara (Inventory of Croton Plant (Codiaeum variegatum L.) in the Backyard of Makaaruyen Village, Modoinding District, South Minahasa Regency,","authors":"Christia Novindy Bororing, S. Mambu, S. Nio","doi":"10.35799/jis.v22i2.41364","DOIUrl":"https://doi.org/10.35799/jis.v22i2.41364","url":null,"abstract":"Tanaman puring (Codiaeum variegatum L.) dikenal sebagai tanaman hias karena bentuk dan warna daun yang beragam. Penelitian ini bertujuan untuk menginventarisasi jenis-jenis puring yang dibudidayakan di pekarangan rumah Desa Makaaruyen, Kecamatan Modoinding, Kabupaten Minahasa Selatan. Pengambilan data dilakukan dengan metode survei langsung dengan menjelajah setiap pekarangan yang mempunyai tanaman puring, mengambil dokumentasi berupa foto, mengamati morfologi tanaman serta mencatat jumlah tanaman untuk setiap jenis tanaman puring yang ditemukan. Bedasarkan hasil penelitian terdapat 25 jenis puring yang terdapat di 13 titik lokasi penelitian, yaitu tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Puring gelatik paling banyak dibudidayakan dibandingkan dengan jenis puring yang lain.Kata kunci: Desa Makaaruyen; gelatik; Puring; Minahasa SelatanABSTRACTCroton (Codiaeum variegatum L.) is known as an ornamental plant because of its various leaf shapes and colors. This study aimed to inventory the types of croton that are cultivated in the home yard of Makaaruyen Village, Modoinding District, South Minahasa Regency. Data collection was conducted by direct survey method by exploring every yard with croton plants, collecting photo documentation, observing plant morphology, and recording the number of each croton plants. Based on the results of the study, there were 25 types of puring found in 13 research locations, namely tokek merah, bor merah, caldium norma, sayap nuri, lele, red gems, gelatik, mentimun, bor emas, patricia, lele kirmizi, walet merah, apel malang, dasi merah, kecapi, lele redline, walet, merah, cabai, kamboja, spageti three color, batang merah, serit, kelabang, dan jet amerika. Gelatik was the most widely cultivated compared to other types of croton.Keywords: Croton; gelatik; Makaaruyen Village; South Minahasa","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90839700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Penerapan Metode SARIMA dalam Model Intervensi Fungsi Step untuk Memprediksi Jumlah Pegunjung Objek Wisata Londa (Application of the SARIMA Method in the Step Function Intervention to Predict the Number of Visitors at Londa Tourism Object)","authors":"Geovani Christie, Djoni Hatidja, Rinancy Tumilaar","doi":"10.35799/jis.v22i2.40961","DOIUrl":"https://doi.org/10.35799/jis.v22i2.40961","url":null,"abstract":"Tujuan dari penelitian ini yaitu menentukan model intervensi pada data jumlah pengunjung di objek wisata Londa dan memprediksi jumlah pengunjung objek wisata Londa pada bulan Desember 2021 sampai Juni 2022 menggunakan model Seasonal Autoregresive Integrated Moving Average (SARIMA). Data yang digunakan adalah data jumlah pengunjung bulanan pada periode bulan Januari 2018 sampai bulan November 2021, yang diperoleh dari pihak pengelolah objek wisata Londa.  Pola data jumlah pengunjung pada objek wisata Londa mengalami fluktuasi yang signifikan, yaitu pada bulan April 2020 (T=28). Model intervensi yang digunakan dalam penelitian ini yaitu fungsi step karena pengaruh yang terjadi berlangsung dalam kurun waktu cukup lama yaitu pada bulan April 2020 sampai November 2021 (T=28 sampai T=47).  Model terbaik yang diperoleh dari hasil analisis data yaitu SARIMA (1,1,0)(1,1,0)[6] dengan orde intervensi b=0, s=5, dan r=2, dengan nilai MAPE dan MAE masing-masing sebesar 4,38% dan 0,397.  Hasil prediksi dari bulan Desember 2021 sampai Juni 2022 secara berturut-turut yaitu 2550, 756, 347, 515, 1585, 1287 dan 2247 orang.  Jumlah pengunjung akan meningkat pada waktu musim liburan yaitu Bulan Desember dan Juni.Kata kunci: Model intervensi; fungsi step; objek wisata Londa,ABSTRACTThe object of this study are to determine the intervention model of the number of visitors at the Londa tourism object and predict the number of visitors at the Londa tourism object for December 2021 to June 2020 using Seasonal Autoregresive Integrated Moving Average (SARIMA) models. The data used is monthly data for the period from January 2018 to November 2021, which was obtained from the manager of the Londa tourist object. The data pattem on the number of visitors at the Londa tourism object experienced significant fluctuations on April 2020 (T=28). The suspected intervention model is a step function because the effect of the intervention that occurred took place over a long period of time, from April 2020 to November 2021 (T=28 to T=47). The best model obtained from the results of data analysis is SARIMA (1,1,0)(1,1,0)[6] with an intervention order of b=0, s=5, and r=2, with MAPE and MAE values are 4,38% and 0,397.  Forecasting results from December 2021 to June 2022 respectively, are 2550, 756, 347, 515, 1585, 1287 and  2247 people.  The number of visitors will increase during the holiday season, i.e. December and June.Keywords: Intervention model; Londa tourism object; step function","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78396837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pengaruh Konsentrasi Probiotik Terhadap Kepadatan dan Komposisi Infusoria yang Ditumbuhkan pada Substrat Daun Pisang (Musa paradisiaca)","authors":"Laras Puput Insanni, S. Sucahyo, D. Cahyaningrum","doi":"10.35799/jis.v22i2.39785","DOIUrl":"https://doi.org/10.35799/jis.v22i2.39785","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui pengaruh konsentrasi probiotik terhadap kepadatan dan komposisi infusoria yang ditumbuhkan pada subtrat daun pisang (Musa paradisiaca). Penelitian ini merupakan penelitian eksperimental Rancangan Acak Lengkap (RAL) satu faktor dengan lima taraf perlakuan yaitu konsentrasi probiotik 0 ml/l, 0,5 ml/l, 1 ml/l, 2 ml/l,  dan 4 ml/l dengan pengulangan sebanyak 4 kali untuk setiap perlakuan.  Pengamatan dilakukan pada hari ke 2, 4, 6, 8, 10, 12, dan 14 untuk memperoleh data berupa kepadatan infusoria, keanekeragaman spesies infusoria beserta jumlah individu per spesies. Data tersebut digunakan untuk mengetahui kepadatan, indeks keanekaragaman (H’), indeks dominansi, serta komposisi spesies infusoria pada setiap perlakuan. Hasil penelitian menunjukkan bahwa konsentrasi probiotik berpengaruh signifikan terhadap kepadatan insufosia (sig 0,010) berdasarkan analisis One Way ANOVA. Indeks keanekaragaman infusoria termasuk dalam kategori rendah pada semua perlakuan. Sejalan dengan hasil tersebut, indeks dominansi pada semua perlakuan berada dalam kategori tinggi. Hasil penelitian menunjukkan bahwa Paramecium sp mendominasi komposisi infusoria pada semua konsentrasi probiotik. Konsentrasi probiotik sebesar 0,5 ml/l,  1 ml/l memicu pertumbuhan Chlorococcum dan digantikan populasi Trichocerca tenuior pada konsentrasi 2 ml/l dan 4 ml/l. Kata kunci: dominansi, infusoria, keanekaragaman, probiotik Probiotic Concentrations Effect on Density and Compotition of Infusoria Grown on Banana Leaf Substrate ABSTRACTThis study aims to determine the effect of probiotic concentration on the density and composition of infusoria grown on banana leaf substrate (Musa paradisiaca). This research is an experimental study in a one-factor Completely Randomized Design (CRD) with five levels of treatment, namely the probiotic concentration 0 ml/l,  0,5 ml/l, 1 ml/l,  2 ml/l and 4 ml/l with 4 repetitions for each treatment. Observations were made on days 2, 4, 6, 8, 10, 12, and 14 to obtain data in the form of infusoria density, infusoria species diversity and the number of individuals per species. These data were used to determine the density, diversity index (H'), dominance index, and the composition of infusoria species in each treatment. The results showed that the concentration of probiotics had a significant effect on the density of insuffocia (sig 0.010) based on One Way ANOVA analysis. The infusoria diversity index was included in the low category in all treatments. In line with these results, the dominance index in all treatments was in the high category. The results showed that Paramecium sp dominated the composition of the infusoria at all concentrations. The probiotic concentration was 0,5ml/l; 1ml/l; stimulated the growth of Chlorococcum and was replaced by Trichocerca tenuior populations at concentrations of 2 ml/l and 4 ml/l.Keywords: dominance, infusoria, diversity, probiotic","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-07-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91376866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Skrining Fitokimia dan Uji Antibakteri Masker Peel-Off Ekstrak Etanol Daun Kalu Burung (Barleria prionitis L.)","authors":"Meriam Gratia Gabriel Wowor, Josua Tampara, Edy Suryanto, L. Momuat","doi":"10.35799/jis.v22i1.38954","DOIUrl":"https://doi.org/10.35799/jis.v22i1.38954","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui kandungan metabolit sekunder yang terdapat pada ekstrak etanol daun kalu burung, membuat masker peel-off berbahan aktif ekstrak etanol daun kalu burung dan menguji aktivitas antibakteri dari ekstrak etanol dan sediaan masker peel-off. Metode yang digunakan adalah preparasi sampel, ekstraksi, uji senyawa fitokimia, pembuatan masker peel-off, uji sifat fisik dan kimia masker meliputi uji organoleptik, stabilitas fisik, lama mengering dan pH, serta uji antibakteri. Hasil penelitian menunjukkan bahwa serbuk daun kalu burung memiliki kadar air sebesar 6.13% dan rendemen ekstrak etanol sebesar 10.34%. Ekstrak etanol daun kalu burung mengandung senyawa metabolit sekunder flavonoid, saponin, tanin, steroid dan alkaloid pada uji Dragendorff. Sediaan masker peel-off memiliki karakteristik fisik dan kimia yang aman digunakan pada kulit. Masker peel-off memiliki kisaran pH antara 5-6 dan lama mengering dengan kisaran antara 23-29 menit. Zona hambat pertumbuhan bakteri untuk ekstrak etanol daun kalu burung sebesar 12.33 mm dan masker peel-off mengandung daun kalu burung sebesar 9.67 mm. Penelitian ini menyimpulkan bahwa daun kalu burung mengandung metabolit sekunder yang memiliki aktivitas antibakteri baik dalam ekstrak etanolnya maupun dalam sediaan masker peel-off ekstrak dan mempunyai sifat fisik dan kimia yang aman.Kata kunci: antibakteri; fitokimia; kalu burung; masker peel-off Phytochemical Screening and Antibacterial Test of Peel-Off Mask with Ethanol Extracts of Kalu Burung Leaves (Barleria prionitis L.).ABSTRACTThis research aims to determine the content of secondary metabolites contained in the ethanol extract of Kalu Burung leaves, to make peel-off masks from ethanol extract of Kalu Burung leaves and to test the antibacterial activity of ethanol extracts and peel-off masks. The methods used were sample preparation, extraction, testing of phytochemical compounds, making peel-off masks, testing the physical and chemical properties of masks such as organoleptic tests, physical stability, drying time and pH, as well as antibacterial tests. The results showed that the Kalu Burung leaf powder had a moisture content of 6.13% and an ethanol extract yield of 10.34%. The ethanol extract of Kalu Burung leaves contains secondary metabolites of flavonoids, saponins, tannins, steroids and alkaloids in the Dragendorff test. The peel-off mask has physical and chemical characteristics which are safe to use. Peel-off masks have a pH range of 5-6 and drying time of 23-29 minutes. The inhibition zone of bacterial growth for ethanol extract was 12.33 mm and the peel-off mask containing Kalu Burung leaves was 9.67 mm. In conclusion, Kalu Burung leaves contain secondary metabolites that have antibacterial activity both in the ethanol extract and in the peel-off extract mask with safe physical and chemical properties.Keywords: antibacterial; kalu burung leaves; peel-off mask; phytochemistry","PeriodicalId":17715,"journal":{"name":"JURNAL ILMIAH SAINS","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86664402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}