Jurnal Sain Veteriner最新文献_第2页

Safety Test for Ethanolic Neem Leaf (Azadirachta indica A. Juss.) Extract on Male Mice (Mus musculus L) Renal Structure 乙醇印楝叶的安全性试验提取液对雄性小鼠肾脏结构的影响

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.46758

Ayu Dwi Lestari, Agung Janika Sitasiwi, Sri Isdadiyanto

{"title":"Safety Test for Ethanolic Neem Leaf (Azadirachta indica A. Juss.) Extract on Male Mice (Mus musculus L) Renal Structure","authors":"Ayu Dwi Lestari, Agung Janika Sitasiwi, Sri Isdadiyanto","doi":"10.22146/jsv.46758","DOIUrl":"https://doi.org/10.22146/jsv.46758","url":null,"abstract":"Neem leaves (Azadirachta indica A. Juss.) are often used as traditional medicine because they contain bioactive compounds such as azadirachtin, nimbidin and nimbolides. Consumption of traditional medicines for long periods can cause side effect on kidney. This study aims to find out the histological structure of glomerular and proximal tubular in the male mice (Mus musculus L.) kidneys after exposure to the ethanolic of neem leaves extract. Completely Randomized Design (CRD) consists of 2 treatment groups with 15 replications was used in this study. The first group is K (only treated with aquadest) and P group (treated by ethanolic neem leaves extrcat at the dosage 14 mg/ kg body weight). The treatment were given orally with a volume of 0.2 mL for 21 days. Feeding and drinking were carried out ad-libitum. At the 22 days, kidney was isolated, weighted and made for histological processed with 5 μm in thickness using paraffin method with Hematoxylin and Eosin staining. The variables observed in this study were kidney weight, water consumption, glomerular diameter, thick of Bowman capsule, diameter of proximal tubule and lumen diameter. Data were analyzed using the t test with a confidence level of 95%. The results of the analysis showed that the treatment has no significant effect (p> 0.05) to kidney weight, water consumption, glomerular diameter, thick of Bowman capsule, diameter of proximal tubules and lumen diameter. The study showed that the use of ethanolic neem leaves extract for 21 days still safe to be use as a traditional medicine.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135005488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Morfologi Pertumbuhan Tulang Toraks Ayam Kampung (Gallus gallus gallus) Pasca-Menetas Sampai Usia Sembilan Minggu 孵化后至九周龄家乡鸡胸骨的形态生长

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.78425

Nur Indah Septriani, Nisrina Salsabila, Ruchianasari Delia Putri, Asifa Bella Ilmy Firdaus, Frida Prasetyo Utami, Hendry Saragih

{"title":"Morfologi Pertumbuhan Tulang Toraks Ayam Kampung (Gallus gallus gallus) Pasca-Menetas Sampai Usia Sembilan Minggu","authors":"Nur Indah Septriani, Nisrina Salsabila, Ruchianasari Delia Putri, Asifa Bella Ilmy Firdaus, Frida Prasetyo Utami, Hendry Saragih","doi":"10.22146/jsv.78425","DOIUrl":"https://doi.org/10.22146/jsv.78425","url":null,"abstract":"Ayam kampung (Gallus gallus gallus) adalah salah satu varietas ayam buras Indonesia. Siklus hidup ayam kampung cepat dengan indikator pertumbuhan dapat dilihat dari pertumbuhan tulangnya. Parameter pertumbuhan ayam salah satunya berasal dari perhitungan hasil pengukuran jarak antar tulang atau sendi. Salah satu bagian yang penting untuk diukur yaitu toraks ayam karena dada adalah salah satu bagian karkas ayam yang mengandung banyak daging. Lingkar, lebar, dan panjang dada pada ayam dapat digunakan untuk menaksir berat daging pada ayam. Oleh karena itu, penelitian ini dilakukan untuk mengetahui panjang tulang dada ayam pada fase pertumbuhan, mulai dari pre-starter sampai ke fase grower. Pengukuran tulang toraks meliputi bagian clavicula, sternum, coracoid, scapula, vertebrae, dan costae menggunakan kaliper dan jangka sorong. Data hasil pengukuran tulang toraks kemudian dianalisis menggunakan metode one-way ANOVA dan Uji Duncan, dengan tingkat kepercayaan 95% (α = 0,05) menggunakan Microsoft Excel dan IBM SPSS Statistics 25. Laju pertumbuhan tulang sternum, coracoid, scapula dan costae mengalami peningkatan pesat sampai minggu ke-5 kemudian mengalami penurunan mulai minggu ke 7. Oleh karena itu pemberian pakan pada minggu awal menetas sampai mingg ke-5 perlu dimaksimalkan dengan memberikan pakan berprotein tinggi untuk memberikan pertumbuhan yang maksimal.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135005495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Profil Hematologi Tikus Putih (Rattus norvegicus) yang Diberi Salep Simplisia Daun Kembang Sepatu (Hibiscus rosa-sinensis L.) Setelah Dipapar Sinar Ultraviolet 小鼠血液学概论(Rattus norvegicus)，采用伞形花丝药膏(芙蓉rosa-sinensis L)。暴露在紫外线下

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.82701

Wayan Gede Ananta Brahmananda, I Gusti Ngurah Sudisma, Anak Agung Sagung Kendran, I Wayan Sudira

{"title":"Profil Hematologi Tikus Putih (Rattus norvegicus) yang Diberi Salep Simplisia Daun Kembang Sepatu (Hibiscus rosa-sinensis L.) Setelah Dipapar Sinar Ultraviolet","authors":"Wayan Gede Ananta Brahmananda, I Gusti Ngurah Sudisma, Anak Agung Sagung Kendran, I Wayan Sudira","doi":"10.22146/jsv.82701","DOIUrl":"https://doi.org/10.22146/jsv.82701","url":null,"abstract":"Sinar ultraviolet (UV) dapat merusak kulit dan mempengaruhi darah sehingga dibutuhkan antioksidan yang terkandung dalam daun kembang sepatu. Tujuan penelitian ini untuk mengetahui pengaruh antioksidan pada salep simplisia daun kembang sepatu (Hibiscus rosa-sinensis L.) terhadap profil hematologi tikus putih (Rattus norvegicus) setelah paparan UV. Rancangan penelitian menggunakan metode rancangan acak lengkap (RAL) dengan sampel 30 ekor tikus putih galur wistar berjenis kelamin betina (100-150 gram) dibagi menjadi 6 perlakuan yang setiap kelompok terdiri dari 5 ekor tikus, yaitu (P0) sampel kontrol negatif tanpa diberi salep dan paparan UV, (P1) sampel kontrol positif tanpa diberi salep dan hanya dipapar UV, dan (P2, P3, P4, dan P5) sampel dengan pemberian salep simplisia daun kembang sepatu masing-masing dengan konsentrasi 10%, 20%, 30%, dan 40% secara topikal pada punggung yang sudah dicukur terlebih dahulu seluas 16 cm2 , kemudian dipapar UV. Spesimen darah diambil satu hari setelah perlakuan melalui vena orbitalis, selanjutnya dilakukan uji hematologi lengkap untuk mengetahui jumlah eritrosit (RBC), nilai hematokrit (HCT), kadar hemoglobin (Hb), dan jumlah leukosit (WBC). Hasil penelitian menunjukkan bahwa pemberian salep simplisia daun kembang sepatu dapat mempertahankan jumlah RBC, nilai HCT, kadar Hb, dan jumlah WBC pada tikus kelompok perlakuan (P2, P3, P4, dan P5) dengan konsentrasi simplisia daun kembang sepatu (10%, 20%, 30%, dan 40%) menunjukkan hasil yang tidak berbeda signifikan (P>0,05) terhadap tikus perlakuan kontrol negatif (P0). Dari hasil penelitian ini dapat disimpulkan, pemberian salep simplisia daun kembang sepatu pada tikus putih setelah dipapar UV dapat mempertahankan jumlah RBC, nilai HCT, kadar Hb, dan jumlah WBC.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135005489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identifikasi Protozoa Gastrointestinal pada Orangutan Sumatera (Pongo abelii) dan Orangutan Kalimantan (Pongo pygmaeus) di Taman Satwa Sumatera Utara 北苏门答腊野生动物园苏门答腊猩猩（Pongo abelii）和婆罗洲猩猩（Pongo pygmaeus）的胃肠道原生动物鉴定

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.74935

Ferina Anggraini, Muhammad Hanafiah, Erdiansyah Rahmi, Farida Athaillah, Teuku Zahrial Helmi, Zainuddin .

{"title":"Identifikasi Protozoa Gastrointestinal pada Orangutan Sumatera (Pongo abelii) dan Orangutan Kalimantan (Pongo pygmaeus) di Taman Satwa Sumatera Utara","authors":"Ferina Anggraini, Muhammad Hanafiah, Erdiansyah Rahmi, Farida Athaillah, Teuku Zahrial Helmi, Zainuddin .","doi":"10.22146/jsv.74935","DOIUrl":"https://doi.org/10.22146/jsv.74935","url":null,"abstract":"Infeksi protozoa merupakan salah satu penyakit parasitik yang umumnya bersifat kronis serta menyebabkan penurunan berat badan. Penelitian ini bertujuan untuk mengetahui protozoa gastrointestinal yang menginfeksi orangutan sumatera (Pongo abelii) dan orangutan kalimantan (Pongo pygmaeus) yang berada di Taman Satwa Sumatera Utara serta protozoa dari genus apa saja yang menginfeksi orangutan tersebut. Sampel pada penelitian ini menggunakan sampel feses yang didapat dari 1 individu orangutan sumatera dan 2 individu orangutan kalimantan. Sampel diambil tiga kali dalam kurun waktu yang berbeda dengan interval 3 minggu. Sampel feses diambil pada pagi hari kemudian ditambahkan dengan formalin 10% dengan perbandingan 1:1. Pemeriksaan sampel dengan menggunakan metode floatasi, metode modified Ziehl-Neelsen, dan metode sedimentasi formol-eter. Hasil dari penelitian ini menunjukkan bahwa 2 dari 3 orangutan di Taman Satwa Sumatera Utara positif terinfeksi protozoa gastrointestinal. Orangutan sumatera bernama Stevee tidak terifeksi protozoa gastrointestinal dan orangutan kalimantan bernama Simba dan Hamidah positif terinfeksi genus protozoa gastrointestinal yaitu, Entamoeba sp., Balantidium sp. dan Cryptosporidium parvum.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135005289","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Penggunaan Al Aiming Reduction Forceps dan Toogling Rode yang Dimodifikasi dalam Penanganan Displasia Coxo Femoral (Hip Displasia) pada Anjing Golden Retriever 在治疗金毛猎犬股骨粗隆发育不良（髋关节发育不良）时使用改良瞄准缩紧钳和趾骨固定器

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.80496

Dhirgo Adji, R. Aria Aji Brameswara

引用次数: 0

Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica) 耐多药菌株exic isolasal Puyuh (Coturnix Coturnix japonica)

Jurnal Sain Veteriner Pub Date : 2023-08-01 DOI: 10.22146/jsv.78592

Wahyu Prihtiyantoro, Agus Purnomo, Sudarisman ., Khusnan Khusnan

{"title":"Multidrug-Resistant Strain ExPEC Isolat Asal Puyuh (Coturnix coturnix japonica)","authors":"Wahyu Prihtiyantoro, Agus Purnomo, Sudarisman ., Khusnan Khusnan","doi":"10.22146/jsv.78592","DOIUrl":"https://doi.org/10.22146/jsv.78592","url":null,"abstract":"AbstractExtraintestinal Escherichia coli (ExPEC) causes colibacillosis in poultry, including quail. ExPEC is a pathogenic E. coli that causes colibacillosis outside the gastrointestinal tract of poultry, in the form of peritonitis, pericarditis, salpingitis, synovitis, osteomyelitis, septicemia, pneumonitis, nephritis, pleurisy, proventriculitis and ventriculitis. In poultry farming antibiotics are used to promote growth and egg production, prevention and treatment of bacterial infections. Antibiotic resistance is a new problem that arises in poultry farming. In this study, 24 isolates of ExPEC strain from quail were used. These isolates did not differentiate sorbitol in the MacConkey (SMAC) sorbitol test assay. Antibiotic resistance was tested based on the inhibition of bacterial growth on Muller Hinnton Agar (MHA) media. 10 types of antibiotics were used to observe their resistance in all isolates. Resistance to Amikacin (87.5%), Ampicillin (87.5%), Ciprofloxacin (100%), Clindamycin (95.83%), Cefoxacin (25.0%), Doxycycline (87.5%), Erythromycin (100%), Gentamicin (95.83%), Penicillin (95.83%) and Tetracycline (83.3%). The isolates had multidrug-resistance between 3 to 10 types of antibiotics. This study showed that ExPEC isolates from quail were resistant to the type of antibiotics tested, so that the use of antibiotics in quail culture should be limited to reduce and prevent the emergence of new antibiotic resistance. Keyword: Escherichia coli, sorbitol-negative, quail, antibiotics resistance","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135005486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Karakteristik Calving Interval pada Sapi Jawa-Brebes di Kabupaten Brebes Jawa Tengah Indonesia

Jurnal Sain Veteriner Pub Date : 2023-04-02 DOI: 10.22146/jsv.77833

Agung Budiyanto, Slamet Hartanto, Imawan Daru Prasetya, Ismu Subroto, Zulfianto Hadratus Asy'ari, Erent Sahat Timotius Sihombing, Yaflet Elifelet Mabel, Annisa Sonia Orintamara, Muhammad Wildan Nasir

引用次数: 0

Proses Penyembelihan dan Waktu Mati Sempurna Sapi Bali sebagai Hewan Kurban di Kabupaten Manokwari 在马奥克瓦里区，巴厘岛牛作为祭祀动物的完美宰杀和死亡过程

Jurnal Sain Veteriner Pub Date : 2023-04-02 DOI: 10.22146/jsv.76415

Priyo Sambodo, Isti Widayati, Dwi Nurhayati, A. Baaka, P. ., J. Palulungan, Rizki Arizona, Noviyanti ., Noveling Inriani, E. K. Suawa, S. D. Rumetor, M. J. Wajo

{"title":"Proses Penyembelihan dan Waktu Mati Sempurna Sapi Bali sebagai Hewan Kurban di Kabupaten Manokwari","authors":"Priyo Sambodo, Isti Widayati, Dwi Nurhayati, A. Baaka, P. ., J. Palulungan, Rizki Arizona, Noviyanti ., Noveling Inriani, E. K. Suawa, S. D. Rumetor, M. J. Wajo","doi":"10.22146/jsv.76415","DOIUrl":"https://doi.org/10.22146/jsv.76415","url":null,"abstract":"Penelitian ini bertujuan untuk mengetahui aspek kesejahteraan hewan berdasarkan tata cara pemisahan dan handling sapi ketika akan disembelih, berapa lama waktu maksimal sapi Bali yang disembelih mati sempurna dan indikator kematian apa yang paling lama hilang setelah sapi disembelih. Penelitian ini dilakukan pada 57 ekor sapi Bali yang disembelih di 5 masjid di Kabupaten Manokwari yang menyelenggarakan penyembelihan hewan kurban. Pengamatan tata cara pemisahan dan handling sapi dilakukan dengan observasi. Waktu henti darah memancar dihitung sejak awal darah memancar sampai tidak lagi memancar. Indikator kematian lain (refleks pupil, refleks kornea, pernafasan ritmik, tonus rahang, tonus lidah, refleks ekor, refleks anus, dan refleks tracak) dihitung setelah waktu henti darah memancar diperoleh. Data yang diperoleh dianalisis secara deskriptif. Data henti darah memancar dihitung rerata dan simpangan bakunya, sedangkan indikator lain disajikan dengan range tiap menit dalam tabel. Pemisahan antara lokasi penempatan dengan penyembelihan dan penggunaan kandang jepit modifikasi untuk handling telah dilakukan disebagian besar lokasi pengamatan. Waktu henti darah memancar pada penelitian ini adalah 2,93 menit. Refleks kornea dan tonus lidah merupakan indikator tercepat yang hilang, yaitu 4-5 menit setelah darah berhenti memancar, kemudian berturut-turut diikuti dengan pernafasan ritmik dan tonus rahang, yaitu masing-masing 5-6 menit serta refleks ekor dan refleks tracak, yaitu 6-7 menit. Refleks anus merupakan indikator terlama yang hilang, yaitu 7-8 menit setelah darah berhenti memancar. Kesimpulan: sebagain besar masjid telah memperhatikan kesejahteraan hewan berdasarkan lokasi dan tata cara penyembelihan. Sapi Bali sebagai hewan kurban di Kabupaten Manokwari mengalami mati sempurna pada menit ke 13,93 dan indikator kematian terakhir yang hilang adalah refleks anus.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75090898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of Newcastle Disease Virus Isolated from Peacocks in Palembang City, South Sumatra 南苏门答腊岛巨港市孔雀分离的新城疫病毒的鉴定

Jurnal Sain Veteriner Pub Date : 2023-04-02 DOI: 10.22146/jsv.76226

L. Angeliya, Akbar Agus Anshori Mussama, S. Alawiyah, T. Guntoro, E. A. Srihanto, Y. P. Kristianingrum, W. Asmara, M. H. Wibowo

{"title":"Characterization of Newcastle Disease Virus Isolated from Peacocks in Palembang City, South Sumatra","authors":"L. Angeliya, Akbar Agus Anshori Mussama, S. Alawiyah, T. Guntoro, E. A. Srihanto, Y. P. Kristianingrum, W. Asmara, M. H. Wibowo","doi":"10.22146/jsv.76226","DOIUrl":"https://doi.org/10.22146/jsv.76226","url":null,"abstract":"Introduction: Newcastle Disease (ND) is an infectious disease in various types of poultry caused by the Newcastle Disease Virus (NDV). Cases of ND in Indonesia have been reported in commercial and backyard chickens, pigeons, ducks and geese, even in eagles and peacocks. Peacock is a wild bird protected by Indonesia's Government Regulation No. 7 of 1999. This study aims to identify, isolate and characterize the NDV molecularly in cases diagnosed as ND in peacocks. Method: Samples were obtained from organs (lungs and spleen) of peacocks which showed neurological symptoms, diarrhoea and then died. Real-time RT-PCR ND was used to identify the cause of death of the peacock. Virus isolation and observation of embryonic changes and death were carried out on embryonic chicken eggs. Sequencing was carried out to characterize the F and HN entire genes of the NDV. The nucleotide sequences were analyzed using MEGA-X software, including amino acid prediction, analysis of genetic variation at the amino acid level, homology and construction of the phylogenic tree. Result: The results of the sample identification were positive for the Newcastle disease virus. Observations of chicken embryos are stunted, have few feathers, are haemorrhagic, and die in less than 60 hours. Virus isolation was obtained with a titer of 26. Molecular analysis showed that the RRQKRF cleavage site pattern in the F gene had homology of 95.8-97.6% and was in the same branching area as the previous ND virus in Indonesia. There were no amino acid mutations at the antigenic site, glycolysis and neutralization epitopes in the HN gene. Conclusion: The virus isolated from the peacock is a velogenic strain of NDV, subgenotype VII.2 and has a close genetic range to the NDV that has been previously reported in commercial and domestic poultry. This result shows that ND is also a threat to protected wild birds.","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77160520","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

KARAGENIN SEBAGAI MODEL INFLAMASI PADA KULIT MENCIT: GAMBARAN MAKROSKOPIK DAN HISTOPATOLOGIS 皮肤炎症模型:宏观和组织学图像

Jurnal Sain Veteriner Pub Date : 2023-04-02 DOI: 10.22146/jsv.62689

S. Widyarini, S. ., Yuli Purwandari Kristianingrum, B. Sutrisno

{"title":"KARAGENIN SEBAGAI MODEL INFLAMASI PADA KULIT MENCIT: GAMBARAN MAKROSKOPIK DAN HISTOPATOLOGIS","authors":"S. Widyarini, S. ., Yuli Purwandari Kristianingrum, B. Sutrisno","doi":"10.22146/jsv.62689","DOIUrl":"https://doi.org/10.22146/jsv.62689","url":null,"abstract":"Penelitian ini bertujuan untuk membuat model inflamasi kulit pada punggung mencit betina galur Swiss dengan menggunakan karagenin dengan berbagai konsentrasi. Pada penelitian ini akan digunakan 45 ekor mencit betina galur Swiss umur 2 bulan yang dibagi dalam tiga kelompok secara acak. Kelompok I diberi karagenin 1%. Kelompok II diberi karagenin 2%, dan kelompok III diberi karagenin 4%. Induksi inflamasi dengan karagenin dilakukan melalui injeksi sub kutan karagenin dengan volume 0,1 mL pada kulit punggung mencit. Sebelum dilakukan injeksi karagenin tebal lipat kulit (skin-fold thickness) diukur dengan menggunakan jangka sorong digital. Selanjutnya setiap jam setelah injeksi sampai dengan jam ke- 6 pasca injeksi. Hewan coba selanjutnya dietanasi dengan cara dislokasi servikalis pada akhir jam ke-6. Organ kulit diambil dengan memotong kulit pada area dimana injeksi karagenin dilakukan dan selanjutnya diawetkan dengan formalin 10% untuk pemeriksaan histopatologis dengan pewarnaan hematoxylin eosin. Analisis data tebal lipat kulit dianalisis dengan Two Ways Anova dan diikuti dengan post hoc test Tukey test. Hasil penelitian memperlihatkan ada perbedaan bermakna pada tebal lipat kulit antar kelompok perlakuan (p<0,05). Injeksi subkutaneuos karagenin 1%, 2% dan 4% meningkatkan tebal lipat kulit sebesar 35%, 50% dan 60% pada jam pertama pasca injeksi (p<0,05). Inflamasi mild dan moderate pada bagian dermis ditemukan pasca injeksi karagenin 1% dan 2%. Infamasi severe pada bagian dermis dan panikulus ditemukan pasca injeksi karagenin 4%. Karagenin dapat dipakai sebagai model inflamasi akut pada kulit punggung mencit. ","PeriodicalId":17708,"journal":{"name":"Jurnal Sain Veteriner","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91541252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0