Journal of virological methods最新文献_第9页

Curcumin inhibits HIV-1 by modulating FOXP3 and suppressing CCR5 via PI3K/AKT and JAK/STAT pathways 姜黄素通过PI3K/AKT和JAK/STAT通路调节FOXP3和抑制CCR5抑制HIV-1

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-06 DOI: 10.1016/j.jviromet.2025.115261

Mengyuan Shi , Qing Li , Wenjin Zheng , Qingya Li , Min Jiang , Jingyi Zhang , Zhe Wang , Lu Qiao , Long Feng

引用次数: 0

Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV) RT-RAA-CRISPR-Cas12a快速、灵敏、视觉检测罗非鱼湖病毒（TiLV）方法的建立

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-18 DOI: 10.1016/j.jviromet.2025.115266

Xiaocong Zheng , Hao Xu , Yun Huang , Xinmei Liu , Songqi Zhu , Hong Liu , Shuai Gao

{"title":"Development of an RT-RAA-CRISPR-Cas12a assay for rapid, sensitive and visual detection of Tilapia Lake Virus (TiLV)","authors":"Xiaocong Zheng , Hao Xu , Yun Huang , Xinmei Liu , Songqi Zhu , Hong Liu , Shuai Gao","doi":"10.1016/j.jviromet.2025.115266","DOIUrl":"10.1016/j.jviromet.2025.115266","url":null,"abstract":"<div><div>In this study，we developed a new, highly efficient, and sequence-specific method for detecting Tilapia Lake Virus (TiLV) based on the clustered regularly interspaced short palindromic repeats (CRISPR) - CRISPR-associated protein 12a (Cas12a) system. TiLV is a highly contagious virus that has caused significant damage to the global aquaculture industry. Specific primers, CRISPR RNA (crRNA), and single-stranded DNA (ssDNA) reporters were designed to detect TiLV genome segment 3, with the ssDNA reporters modified at the 5’ and 3’ ends with fluorophore and quencher groups, respectively. The assay showed no cross-reactivity with other bacterial and viral pathogens in fish. The detection limit was 9.10 copies per reaction for recombinant plasmid standards and 91.82 fg/μL for TiLV RNA, demonstrating high sensitivity. The reverse transcription recombinase aided amplification (RT-RAA) coupled CRISPR/Cas12a method showed 100 % concordance with the standard fluorescence method, indicating its accuracy and suitability for clinical testing. This study innovatively combined the RT-RAA technique with the CRISPR/Cas12a reaction system, offering a new diagnostic method for TiLV that is fast, portable, highly specific, and sensitive. This enables on-site rapid screening for TiLV, ensuring aquaculture safety and the secure circulation of aquatic animal products.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115266"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145103055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples SYBR绿色实时荧光定量PCR检测动物口咽标本SARS-CoV-2的性能评价

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-05 DOI: 10.1016/j.jviromet.2025.115259

María Emilia Bravi , Nadia Analía Fuentealba , Natalia Brasso , Guillermo Hernan Sguazza , Marcelo Ricardo Pecoraro , Carlos Javier Panei

{"title":"Performance evaluation of a SYBR Green-based real-time quantitative PCR for SARS-CoV-2 detection from animal oropharyngeal samples","authors":"María Emilia Bravi , Nadia Analía Fuentealba , Natalia Brasso , Guillermo Hernan Sguazza , Marcelo Ricardo Pecoraro , Carlos Javier Panei","doi":"10.1016/j.jviromet.2025.115259","DOIUrl":"10.1016/j.jviromet.2025.115259","url":null,"abstract":"<div><div>The global emergence of SARS-CoV-2 has highlighted the need for rapid, sensitive, and affordable diagnostic tools, not only for human health but also for animal surveillance within a One Health framework. This study aimed to evaluate the performance of a SYBR Green-based real-time quantitative PCR (qPCR) assay for the detection of SARS-CoV-2 from animal samples, focusing on domestic dogs and cats. A total of 140 oropharyngeal swab samples were collected and analyzed using primers targeting a 139-bp fragment of the <em>N</em> gene of SARS-CoV-2. The assay conditions were optimized through gradient PCR, primer concentration adjustment, and melting curve analysis. Cloning and quantification of the target gene allowed the determination of the limit of detection (LOD), which was estimated at 2.1 × 10<sup>2</sup> copies/µL. Among the samples tested, 13 were positive for SARS-CoV-2, confirmed by a commercial probe-based qPCR. The assay here evaluated demonstrated high specificity, with no cross-reactivity to canine or feline coronaviruses, and had a highly linear standard curve of 0.977 (R² = 0.997) with a value range of quantification cycle (Cq) from 9.25 to 34.89. In addition, it exhibited a 2-log increase in sensitivity compared to conventional PCR. The intra- and inter-assay coefficients of variation were below 1.1 % and 2 %, respectively, confirming high reproducibility. These results support the use of SYBR Green real-time qPCR as a cost-effective and reliable alternative for SARS-CoV-2 detection from animal samples, particularly in resource-limited settings, serving as a tool for epidemiological control of SARS-CoV-2 infection in animal populations.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115259"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145015801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development and optimisation of influenza C and influenza D pseudotyped viruses 流感C型和流感D型假病毒的研制和优化

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-08-23 DOI: 10.1016/j.jviromet.2025.115243

Maria Giovanna Marotta , Martin Mayora Neto , Janet Daly , Meshach Maina , Pauline van Diemen , Helen Everett , Maria Stella Lucente , Michele Camero , Emanuele Montomoli , Claudia Maria Trombetta , Kelly da Costa , Nigel J. Temperton

{"title":"Development and optimisation of influenza C and influenza D pseudotyped viruses","authors":"Maria Giovanna Marotta , Martin Mayora Neto , Janet Daly , Meshach Maina , Pauline van Diemen , Helen Everett , Maria Stella Lucente , Michele Camero , Emanuele Montomoli , Claudia Maria Trombetta , Kelly da Costa , Nigel J. Temperton","doi":"10.1016/j.jviromet.2025.115243","DOIUrl":"10.1016/j.jviromet.2025.115243","url":null,"abstract":"<div><div>To facilitate the study of influenza C (ICV) and influenza D (IDV) viruses, we generated lentiviral pseudotyped viruses (PVs) expressing the hemagglutinin-esterase fusion (HEF) glycoprotein from ICV (C/Minnesota/33/2015) and IDV (D/Swine/Italy/199724–3/2015, D/Bovine/France/5920/2014, and D/Bovine/Ibaraki/7768/2016). The production of these PVs was optimised using different amount of human airway trypsin-like (HAT) protease to enhance HEF maturation, and the transduction efficiency was evaluated in multiple cell lines. Using these PVs, we established a pseudovirus-based microneutralisation (pMN) assay to measure neutralising antibody responses and adapted an esterase activity assay to evaluate PV. Specific antisera neutralised PVs but failed to inhibit esterase activity. These findings confirm that ICV and IDV PVs provide a scalable, sensitive, and safe tool for antiviral screening, and sero-epidemiological research.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115243"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144916780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany 德国苹果胶木病毒1号和2号新检测引物的研制及筛选。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-08-23 DOI: 10.1016/j.jviromet.2025.115241

Christine Seinsche, Constanze Berwarth, Marlen Brodbeck, Kerstin Zikeli, Andrej Vondran, Lennard Kleemann, Stefanie Thomas, Wilhelm Jelkmann

{"title":"Development of novel detection primers and screening for apple rubbery wood virus 1 and 2 in Germany","authors":"Christine Seinsche, Constanze Berwarth, Marlen Brodbeck, Kerstin Zikeli, Andrej Vondran, Lennard Kleemann, Stefanie Thomas, Wilhelm Jelkmann","doi":"10.1016/j.jviromet.2025.115241","DOIUrl":"10.1016/j.jviromet.2025.115241","url":null,"abstract":"<div><div>Apple rubbery wood disease is globally distributed and affects primarily apples and pears. A characteristic symptom of the disease, which is caused by the two viruses Apple rubbery wood virus (ARWV) 1 and 2 respectively, is enhanced branch and twig flexibility leading to reduced fruit yields. In this study, a highly sensitive RT-PCR detection system was established for reliable and specific detection of all ARWV-1 and ARWV-2 genomic segments, S, M and L, and their variants. Phloem was identified as convenient tissue for reliable ARWV detection. The detection system was applied to 1059 apple samples from German commercial orchards, experimental plots, and variety collections to investigate ARWV distribution across Germany. Overall, ARWV-1, ARWV-2, or mixed infections were identified in 14.2 % of the samples. Commercial orchard samples from thirteen federal states were tested. In eleven federal states ARWV-1 infections ranging from 2.1 % to 16.7 % were detected, in five federal states the ARWV-2 infection status varied from 1.6 % to 12.4 % and in one federal state ARWV-1 and -2 mixed infections were observed. Within the commercial orchard sample set, 47 different apple varieties were tested. Several varieties, highly relevant for apple growers, show ARWV infection rates between 5 % and 10 %. This study provides the first comprehensive overview of ARWV occurrence in German apple orchards and underscores the importance of sensitive diagnostic tools for effective disease monitoring, analysis of planting material and future management strategies.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115241"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144959158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP RT-LAMP实时检测瓜绿黄病毒。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-09 DOI: 10.1016/j.jviromet.2025.115262

Ángela Martínez-Fernández , Josemaría Delgado-Martín , Esmeralda Sanahuja-Edo , Ana Alfaro-Fernández , José Manuel Estévez , Isabel Font-San-Ambrosio , Luis Galipienso , Luis Rubio

{"title":"Real-time detection of cucurbit chlorotic yellows virus by RT-LAMP","authors":"Ángela Martínez-Fernández , Josemaría Delgado-Martín , Esmeralda Sanahuja-Edo , Ana Alfaro-Fernández , José Manuel Estévez , Isabel Font-San-Ambrosio , Luis Galipienso , Luis Rubio","doi":"10.1016/j.jviromet.2025.115262","DOIUrl":"10.1016/j.jviromet.2025.115262","url":null,"abstract":"<div><div>Cucurbit chlorotic yellows virus (CCYV) of the genus <em>Crinivirus</em> and family <em>Closteroviridae</em>, is an emerging infectious agent transmitted by whiteflies that mainly infects cucumber, melon and watermelon plants. Here, we developed a procedure based on reverse transcription (RT) followed by loop-mediated isothermal amplification (LAMP) to detect CCYV in real-time by using a fluorescent dye and incubating at 65 °C. This method detected CCYV in RNA extracts at about 10 min and in nylon membrane-filtered crude extracts between 55 and 95 min. The detection was sensitive, ten times higher than RT followed by polymerase chain reaction (PCR) and specifically detecting different isolates of CCYV without cross-reaction with other viruses of the same genus. This procedure enables the simultaneous analyses of multiple samples, allowing for rapid, sensitive and specific detection of CCYV. By using membrane-filtered crude extracts, RNA purification is unnecessary, so the whole process can be performed in the field with a portable heater and fluorometer, facilitating a rapid response for disease control.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115262"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145040560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces 树鼩作为潜在宿主：首次在马来西亚树鼩粪便中检测到血清2型登革热病毒

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-08-31 DOI: 10.1016/j.jviromet.2025.115256

Zhen Yun Siew , Chen Zhe Tang , Siti Nor Asma Musa , Isaac Seow , Nur Alia Johari , Pooi Pooi Leong , Siew Tung Wong , Kenny Voon

{"title":"Treeshrews as a potential reservoir: First detection of dengue virus serotype 2 in Malaysian treeshrew faeces","authors":"Zhen Yun Siew , Chen Zhe Tang , Siti Nor Asma Musa , Isaac Seow , Nur Alia Johari , Pooi Pooi Leong , Siew Tung Wong , Kenny Voon","doi":"10.1016/j.jviromet.2025.115256","DOIUrl":"10.1016/j.jviromet.2025.115256","url":null,"abstract":"<div><div>Arboviruses are transmitted to humans and animals by arthropods and can be fatal. Dengue fever remains a major mosquito-borne disease in tropical regions, primarily spread by <em>Aedes aegypti</em> and <em>Aedes albopictus</em>. Despite vector control and vaccine efforts, dengue virus (DENV) continues to pose serious public health challenges in Malaysia. While non-human primates are known reservoirs in sylvatic cycles, the role of other mammals like treeshrews (<em>Tupaia glis</em>) is poorly understood. This study screened wild treeshrews in suburban Semenyih, Malaysia, for DENV and its serotype. From 2023–2024, fecal and urine samples were collected and pooled for molecular screening. Viral RNA was extracted and tested via RT-PCR targeting the Capsid-Premembrane (C-prM) region. Of 11 samples, three (27.3 %) were positive for DENV-2. Sequence analysis revealed the cosmopolitan genotype II, typically linked to human transmission, rather than sylvatic strains. Virus isolation in Vero and C6/36 cells showed cytopathic effects, though contamination hampered results. These findings suggest treeshrews may serve as incidental reservoirs or amplifying hosts of DENV-2, highlighting the need for wildlife surveillance to better understand dengue transmission and guide public health responses.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115256"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144933145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection 体外模拟人背根神经节神经元对HSV-1感染的感觉反应的gcamp6钙显像

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-11 DOI: 10.1016/j.jviromet.2025.115264

Yu-Chih Chen , Brandon Bullock , Daniel I. Ogbeh , Jinglu Ai , Justin Okoh , Jia Liu , Yuhao Qiang , S. Victor Hsia

{"title":"In vitro modeling of human dorsal root ganglion neurons for GCaMP6-based calcium imaging of sensory responses to HSV-1 infection","authors":"Yu-Chih Chen , Brandon Bullock , Daniel I. Ogbeh , Jinglu Ai , Justin Okoh , Jia Liu , Yuhao Qiang , S. Victor Hsia","doi":"10.1016/j.jviromet.2025.115264","DOIUrl":"10.1016/j.jviromet.2025.115264","url":null,"abstract":"<div><div>Dorsal root ganglion (DRG) neurons play a pivotal role in transmitting sensory information from the periphery to the central nervous system, mediating diverse stimuli such as pain, touch, and temperature. Despite advances, translating findings from rodent models to human applications remains challenging due to species-specific differences, necessitating reliable human DRG neuron models. The immortalized human DRG neuronal cell line HD10.6, derived from embryonic DRG cells and capable of differentiating into functional nociceptive-like neurons, offers a promising in vitro system for studying sensory neuron biology and drug screening. This study explores the utility of GCaMP6s, a genetically encoded calcium indicator, as a molecular tool for imaging sensory activation in HD10.6 cells. To establish HD10.6 as a robust human DRG model, we constructed and characterized adeno-associated virus (AAV9) vectors for efficient GCaMP6s delivery. Differentiated HD10.6 cells were efficiently transduced, and calcium dynamics were validated to assess functional responses to sensory stimuli. The results showed that AAV9 serotype was sufficient to infect HD10.6 and the GCaMP6s was successfully introduced into the cells. The HD10.6-GCaMP6s responded to capsaicin well under the appropriate condition. A series of viral infection studies indicated that herpesvirus HSV-1 triggered robust calcium influx within 5 min after the exposure to the virus. Our findings highlight the potential of GCaMP6s-expressing HD10.6 cells as a high-throughput platform for studying nociception, neuronal signaling, host cell responses to viruses, and therapeutic interventions, bridging the gap between preclinical research and clinical applications.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115264"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145045611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania 利用flongle流式细胞通过纳米孔测序检测和表征新生儿巨细胞病毒：在宾夕法尼亚州费城的初步研究

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-08-24 DOI: 10.1016/j.jviromet.2025.115245

Alvaro Proaño , Joe Chan , Gabrielle C. Galchen , Mian Umair Ahsan , Robert H. Gilman , Kenneth P. Smith , Kai Wang , Dustin D. Flannery

{"title":"Detection and characterization of neonatal cytomegalovirus through nanopore sequencing using flongle flow cells: Pilot study in Philadelphia, Pennsylvania","authors":"Alvaro Proaño , Joe Chan , Gabrielle C. Galchen , Mian Umair Ahsan , Robert H. Gilman , Kenneth P. Smith , Kai Wang , Dustin D. Flannery","doi":"10.1016/j.jviromet.2025.115245","DOIUrl":"10.1016/j.jviromet.2025.115245","url":null,"abstract":"<div><h3>Background</h3><div>Cytomegalovirus (CMV) remains a significant infection in neonates and its early detection can aid with further treatment (antiviral, audiology). However, current diagnostics do not provide genetic information.</div></div><div><h3>Objective</h3><div>We explored the use of the portable and comprehensive sequencing method from Oxford Nanopore Technologies, utilizing low-cost Flongle flow cells to detect and perform sequence-level characterization of neonatal urine samples that tested positive for CMV by PCR.</div></div><div><h3>Study design</h3><div>We performed a pilot study based on a retrospective cohort study of neonates who were positive for CMV by PCR, who were admitted at two birth hospitals in Philadelphia, PA. We leveraged deep and long-read sequencing results to analyze the reads in two forms: by comparing them against a reference-based strain and by reconstructing the genome through de novo assembly with phylogenetic tree analysis.</div></div><div><h3>Results</h3><div>We assayed seven clinical samples, including a positive and negative control sample, from newborns ranging from 23 weeks' gestation to term, with testing performed for microcephaly, hearing test results, small gestational age, and thrombocytopenia. Each sample showed multiple differences compared to the reference strain, and the phylogenetic tree analysis of the de novo assembly depicted the genetic diversity of the samples.</div></div><div><h3>Conclusion</h3><div>This pilot study shows that nanopore sequencing with low-cost Flongle flow cells can detect and characterize CMV strains from clinical neonatal urine samples. This, coupled with current screening and diagnostic criteria, could further our genomic understanding of neonatal CMV, such as viral genome diversity, genotype-phenotype associations, and spread of strains.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115245"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of dead-end hollowfiber ultrafilter options for enumerating somatic and F+ coliphage in ambient waters and wastewater 对环境水和废水中体细胞和F+噬菌体计数的无端中空纤维超滤选择的评价。

IF 1.6 4区医学

Journal of virological methods Pub Date : 2026-01-01 Epub Date: 2025-09-15 DOI: 10.1016/j.jviromet.2025.115267

Brian R. McMinn, Julie Kelleher, Asja Korajkic

{"title":"Evaluation of dead-end hollowfiber ultrafilter options for enumerating somatic and F+ coliphage in ambient waters and wastewater","authors":"Brian R. McMinn, Julie Kelleher, Asja Korajkic","doi":"10.1016/j.jviromet.2025.115267","DOIUrl":"10.1016/j.jviromet.2025.115267","url":null,"abstract":"<div><div>Coliphage are viral indicators of fecal contamination in water while acting as possible proxies for enteric viral pathogens. Depending on contamination levels, coliphage could be present at concentrations necessitating the use of concentrating filters. Hollow-fiber ultrafilters (HFUF) such as Asahi Kasei Rexeed have successfully concentrated coliphage in a dead-end setup (D-HFUF) from environmental waters and are recommended within United States Environmental Protection Agency (USEPA) Method 1642. Asahi Kasei Rexeed are not available within the United States, so replacement filters need to be identified. Additionally, coliphage methods lack recommendations for sample holding times to prevent variability in coliphage concentrations between sample collection and analysis. We compared HFUFs, the Fresenius F160NRE and the Elisio-15H, to the Asahi Kasei Rexeed 15S to determine their efficacy in recovering somatic and F+ coliphage from river, lake, marine, and wastewater. A 2 L volume of each matrix (river, lake, marine, and final effluent [<em>n</em> = 10 each]), were concentrated using D-HFUF for each filter brand with coliphage enumerated using the single agar layer (SAL) assay. There was no significant difference in performance between the three filters regardless of sample matrix (p > 0.05). To establish sample holding times, each water matrix (stored at 4ºC) was analyzed on a weekly basis for endogenous coliphage. In wastewater, significant decay occurred within 48 h of collection (<em>P</em> value range: 0.0175–0.0006), while in other matrices, coliphages were stable ≥ 6 days. In this study, we identified replacement HFUFs and pertinent information regarding sample holding times for coliphage monitoring efforts moving forward.</div></div>","PeriodicalId":17663,"journal":{"name":"Journal of virological methods","volume":"339 ","pages":"Article 115267"},"PeriodicalIF":1.6,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145081215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0